Pyridostatin (RR82) Trifluoroacetate Salt

Catalog No.S7444

For research use only.

Pyridostatin Trifluoroacetate Salt (RR82) is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.

Pyridostatin (RR82) Trifluoroacetate Salt Chemical Structure

CAS No. 1472611-44-1

Selleck's Pyridostatin (RR82) Trifluoroacetate Salt has been cited by 7 Publications

Purity & Quality Control

Choose Selective G-quadruplex Inhibitors

Biological Activity

Description Pyridostatin Trifluoroacetate Salt (RR82) is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.
Targets
G-quadruplexe [1]
(Cell-free assay)
490 nM(Kd)
In vitro

Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. [2] Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MRC5 MnzpR5l1d3SxeHnjbZR6KGG|c3H5 NVixTJRlPzJiaILz M3TjT2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1TSzViY3XscJMh[XO|ZYPz[YQh[XNiY3XscEBoem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUWuN|jPxE1w M2HmblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{KzOFkyLz5{OUOyN|Q6OTxxYU6=
KB-3-1 NHXCdpJyUFSVIHHzd4F6 MkL0VE1odHmlb4Dyc5RmcW5ic4Xid5Rz[XSnczDp[IVvfGmoaXXkJIlvKEuELUOtNUBi\GWwb3PhdoNqdm:vYTDj[YxtKGyrbnWsJJFJXFNidHjldoFx\XW2aXOgcIljemG{eTDzZ5Jm\W5? NH;lN2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUWxOVI5PCd-M{G1NVUzQDR:L3G+
In vivo

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: MRC-5–SV40 cells and various cancer cell lines
  • Concentrations: 10 μM
  • Incubation Time: 48 h
  • Method:

    Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(140.7 mM)
Water 100 mg/mL
(140.7 mM)
Ethanol ''''30 mg/mL

Chemical Information

Molecular Weight 710.7
Formula

C31H32N8O5·C2HF3O2

CAS No. 1472611-44-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C2C(=C1)C(=CC(=N2)NC(=O)C3=CC(=CC(=N3)C(=O)NC4=NC5=CC=CC=C5C(=C4)OCCN)OCCN)OCCN.C(=O)(C(F)(F)F)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Pyridostatin (RR82) Trifluoroacetate Salt | Pyridostatin (RR82) Trifluoroacetate Salt supplier | purchase Pyridostatin (RR82) Trifluoroacetate Salt | Pyridostatin (RR82) Trifluoroacetate Salt cost | Pyridostatin (RR82) Trifluoroacetate Salt manufacturer | order Pyridostatin (RR82) Trifluoroacetate Salt | Pyridostatin (RR82) Trifluoroacetate Salt distributor