Home Endocrinology & Hormones Estrogen/progestogen Receptor agonist Psoralidin For research use only.

Psoralidin

Psoralidin, a naturally occurring coumestan isolated from the fractions of organic solvents such as ethylacetate, hexane, or n-butanol of the seed extract of Psoralea corylifolia L., has a variety of biological activities such as anticancer, antioxidant, antibacterial, antidepressant, anti-inflammatory activities, and regulation of insulin signaling. It is an agonist for both estrogen receptor (ER)α and ERβ with binding affinities (IC50s) of 1.03 and 24.6 μM, respectively.

Psoralidin Chemical Structure

Psoralidin Chemical Structure

CAS: 18642-23-4

Purity & Quality Control

Batch: S546401 DMSO] 67 mg/mL] false] Ethanol] 1 mg/mL] false] Water] Insoluble] false Purity: 99.96%
99.96

Psoralidin Related Products

Signaling Pathway

Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Psoralidin, a naturally occurring coumestan isolated from the fractions of organic solvents such as ethylacetate, hexane, or n-butanol of the seed extract of Psoralea corylifolia L., has a variety of biological activities such as anticancer, antioxidant, antibacterial, antidepressant, anti-inflammatory activities, and regulation of insulin signaling. It is an agonist for both estrogen receptor (ER)α and ERβ with binding affinities (IC50s) of 1.03 and 24.6 μM, respectively.
Targets
ERα [1]
(Cell-free)		 ERβ [1]
(Cell-free)
1.03 μM 24.6 μM
In vitro
In vitro Psoralidin has been characterized as a full ER agonist, which activates the classical ER-signaling pathway in both ER-positive human breast and endometrial cell lines as well as non-human cultured cells transiently expressing either ERα or ERβ. Psoralidin was also able to induce the endogenous estrogen-responsive gene, pS2, in human breast cancer cells MCF-7. The IC50 values for psoralidin to replace the binding of E2 to both receptors were 1.03 and 24.6 μM, respectively. Psoralidin is able to bind to both receptors within the micromolar range and has preferential affinity for ERα over ERβ[1].
Cell Research Cell lines MCF-7 cells
Concentrations 10 μM
Incubation Time 24 h
Method

MCF-7 cells were cultured in estrogen-free RPMI 1640 media for 4 days before transfection, and plated (4 × 105 cells/well) in triplicate onto a 12-well plate. Cells were transiently transfected with pERE-luciferase plasmid (0.5 μg/well) and with an internal control plasmid pRL-Tk (0.1 μg/well) using Lipofectamine 2000 Reagent. ERE-luciferase plasmid contains three copies of the Xenopus laevis vitellogenin A2 ERE upstream of fire fly luciferase. The pRL-Tk plasmid contains a cDNA encoding Renilla luciferase. At 24 h post-transfection, cells were treated DMSO, various concentrations of E2, psoralidin, ICI, or appropriate combination of two compounds and incubated for another 24 h. The cells were harvested with Passive Lysis buffer. Luciferase activity present in the cell lysates was measured.
In Vivo
In vivo Acute and subchronic administrations of psoralidin produced a significant reduction in immobility time in the mouse forced swimming test. Psoralidin dose-dependently increased swimming and failed to affect climbing[2].
Animal Research Animal Models Male ICR strain of mice
Dosages 20, 40 and 60 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 336.34 Formula

C20H16O5
CAS No. 18642-23-4 SDF --
Smiles CC(=CCC1=CC2=C(C=C1O)OC(=O)C3=C2OC4=C3C=CC(=C4)O)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.2 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Psoralidin | Psoralidin supplier | purchase Psoralidin | Psoralidin cost | Psoralidin manufacturer | order Psoralidin | Psoralidin distributor