Propidium Iodide

Catalog No.S6874 Batch:S687401

Technical Data

Formula

C₂₇H₃₄N₄.₂I

Molecular Weight

668.39

CAS No.

25535-16-4

Solubility (25°C)*

In vitro

DMSO

100 mg/mL (149.61 mM)

Water

10 mg/mL (14.96 mM)

Ethanol

1 mg/mL (1.49 mM)

In vivo (Add solvents to the product individually and in order)

Clear solution	saline	10.000mg/ml	Taking the 1 mL working solution as an example, add 10 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Homogeneous suspension for Oral and Intraperitoneal Injection. Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Propidium iodide (PI) is a red-fluorescent DNA stain which penetrates only damaged cellular membranes. Propidium iodide (PI) is described for use in the determination of cell viability in cell suspension.

Protocol (from reference)

References

Selleck's Propidium Iodide has been cited by 18 publications

Genetic disruption of the circadian gene Bmal1 in the intestinal epithelium reduces colonic inflammation [ EMBO Rep, 2025, 10.1038/s44319-025-00464-y]	PubMed: 40307620
Oxypeucedanin alleviates cisplatin-induced intestinal toxicity through impairing caspase-3/gasdermin E signaling [ J Vet Med Sci, 2025, 10.1292/jvms.24-0488]	PubMed: 40024724
Inhibition of Selenoprotein I promotes ferroptosis and reverses resistance to platinum chemotherapy by impairing Akt phosphorylation in ovarian cancer [ MedComm (2020), 2024, 5(12):e70033]	PubMed: 39669976
HSPA12A promotes c-Myc lactylation-mediated proliferation of tubular epithelial cells to facilitate renal functional recovery from kidney ischemia/reperfusion injury [ Cell Mol Life Sci, 2024, 81(1):404]	PubMed: 39277835
USP13 regulates ferroptosis in chicken follicle granulosa cells by deubiquitinating ATG7 [ Poult Sci, 2024, 103(11):104209]	PubMed: 39214053
Oncolytic Parapoxvirus induces Gasdermin E-mediated pyroptosis and activates antitumor immunity [ Nat Commun, 2023, 14(1):224]	PubMed: 36641456
Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity [ Int J Mol Sci, 2023, 24(10)8651]	PubMed: 37239999
Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity [ Int J Mol Sci, 2023, 24(10)8651]	PubMed: 37239999
Circular RNA circ_0021001 regulates miR-148b-3p/GREM1 axis to modulate proliferation and apoptosis of vascular smooth muscle cells [ Metab Brain Dis, 2022, 37(6):2027-2038]	PubMed: 35689751
Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1 [ Reprod Sci, 2022, 10.1007/s43032-022-00925-y]	PubMed: 35378711

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.