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N-Succinimidyl-S-acetylthioacetate (SATA)

Cat.No.S0057

N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent which introduces thiol-groups into protein molecules. This compound adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form.
N-Succinimidyl-S-acetylthioacetate (SATA) Chemical Structure

Chemical Structure

Molecular Weight: 231.23

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S005701 DMSO]46 mg/mL]false]Ethanol]4 mg/mL]false]Water]Insoluble]false Purity: 99%
99

Chemical Information, Storage & Stability

Molecular Weight 231.23 Formula

C8H9NO5S

 Storage (From the date of receipt) 3 years -20°C powder
CAS No. 76931-93-6 -- Storage of Stock Solutions

Synonyms N/A Smiles CC(=O)SCC(=O)ON1C(=O)CCC1=O

Solubility

In vitro
Batch:

DMSO : 46 mg/mL (198.93 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro

SATA allows the introduction of sulfhydryl groups into proteins by the reaction of its active NHS ester end with amino groups, without affecting the indigenous disulfides of the protein.[2]
In vivo

Due to antibody degradation, and spontaneous precipitation of the SATA conjugated antibody upon storage, this compound is not a suitable bifunctional chelating agent (BCA).[1]
References

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