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N-Succinimidyl-S-acetylthioacetate (SATA)

N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent which introduces thiol-groups into protein molecules. N-Succinimidyl-S-acetylthioacetate (SATA) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form.

N-Succinimidyl-S-acetylthioacetate (SATA) Chemical Structure

N-Succinimidyl-S-acetylthioacetate (SATA) Chemical Structure

CAS: 76931-93-6

Purity & Quality Control

Batch: S005701 DMSO] 46 mg/mL] false] Ethanol] 4 mg/mL] false] Water] Insoluble] false Purity: 99%
99

N-Succinimidyl-S-acetylthioacetate (SATA) Related Products

Biological Activity

Description N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent which introduces thiol-groups into protein molecules. N-Succinimidyl-S-acetylthioacetate (SATA) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form.
In vitro
In vitro

SATA allows the introduction of sulfhydryl groups into proteins by the reaction of its active NHS ester end with amino groups, without affecting the indigenous disulfides of the protein.[2]
Cell Research Cell lines A-431, MRC-5 cell lines
Concentrations --
Incubation Time 4 h
Method

The conjugation procedure is performed through derivatization of nimotuzumab with SATA followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Cells are seeded at 80% confluence in chamber slides. After 24 h of culture, cells are incubated with 40 mL of a dilution 1:50 of liposomes or immunoliposomes (ILs) and then incubated for 4 h at 37 ℃. Unbound liposomes or ILs are removed by washing (3×) with PBS 1×(pH 7.4) and fixed with 3.7% paraformaldehyde. The samples are washed again with PBS 1×and mounted with vectashield mounting medium.
In Vivo
In vivo

Due to antibody degradation, and spontaneous precipitation of the SATA conjugated antibody upon storage, SATA is not a suitable bifunctional chelating agent (BCA).[1]

Chemical Information & Solubility

Molecular Weight 231.23 Formula

C8H9NO5S
CAS No. 76931-93-6 SDF --
Smiles CC(=O)SCC(=O)ON1C(=O)CCC1=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 46 mg/mL ( (198.93 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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