N-Succinimidyl-S-acetylthioacetate (SATA)

Catalog No.S0057 Batch:S005701

Technical Data

Formula	C₈H₉NO₅S
Molecular Weight	231.23	CAS No.	76931-93-6
Solubility (25°C)*	In vitro	DMSO	46 mg/mL (198.93 mM)
		Ethanol	4 mg/mL (17.29 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	N-Succinimidyl-S-acetylthioacetate (SATA) is a protein modification agent which introduces thiol-groups into protein molecules. N-Succinimidyl-S-acetylthioacetate (SATA) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form.
In vitro	SATA allows the introduction of sulfhydryl groups into proteins by the reaction of its active NHS ester end with amino groups, without affecting the indigenous disulfides of the protein.^[2]
In vivo	Due to antibody degradation, and spontaneous precipitation of the SATA conjugated antibody upon storage, SATA is not a suitable bifunctional chelating agent (BCA).^[1]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines A-431, MRC-5 cell lines Concentrations -- Incubation Time 4 h Method The conjugation procedure is performed through derivatization of nimotuzumab with SATA followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Cells are seeded at 80% confluence in chamber slides. After 24 h of culture, cells are incubated with 40 mL of a dilution 1:50 of liposomes or immunoliposomes (ILs) and then incubated for 4 h at 37 ℃. Unbound liposomes or ILs are removed by washing (3×) with PBS 1×(pH 7.4) and fixed with 3.7% paraformaldehyde. The samples are washed again with PBS 1×and mounted with vectashield mounting medium.

Cell Assay:^{^[2]}

Cell lines
A-431, MRC-5 cell lines
Concentrations
--
Incubation Time
4 h
Method
The conjugation procedure is performed through derivatization of nimotuzumab with SATA followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Cells are seeded at 80% confluence in chamber slides. After 24 h of culture, cells are incubated with 40 mL of a dilution 1:50 of liposomes or immunoliposomes (ILs) and then incubated for 4 h at 37 ℃. Unbound liposomes or ILs are removed by washing (3×) with PBS 1×(pH 7.4) and fixed with 3.7% paraformaldehyde. The samples are washed again with PBS 1×and mounted with vectashield mounting medium.

References

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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