Home Autophagy LC3 chemical LC3-mHTT-IN-AN1 For research use only.

LC3-mHTT-IN-AN1

LC3-mHTT-IN-AN1 is a mHTT-LC3 linker compound that interacts with both mutant huntingtin protein (mHTT) and LC3B. LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured Huntington disease (HD) mouse neurons.

LC3-mHTT-IN-AN1 Chemical Structure

LC3-mHTT-IN-AN1 Chemical Structure

CAS: 486443-73-6

Purity & Quality Control

Batch: S077801 DMSO] 82 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.85%
99.85

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Biological Activity

Description LC3-mHTT-IN-AN1 is a mHTT-LC3 linker compound that interacts with both mutant huntingtin protein (mHTT) and LC3B. LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured Huntington disease (HD) mouse neurons.
Targets
LC3 [1] mHTT [1]
In vitro
In vitro

LC3-mHTT-IN-AN1 reduces the levels of mHTT in an allele-selective manner in cultured HD mouse neurons.[1]
Cell Research Cell lines mouse primary cultured neurons and iPS-cell-derived neurons
Concentrations 10, 50, 100, and 300 nM
Incubation Time --
Method

LC3-mHTT-IN-AN1 was diluted in culture medium to 10×concentrations and added to the plated cells: for primary cultured neurons and iPS-cell-derived neurons, LC3-mHTT-IN-AN1 was added 5 days after plating; The cells were then collected 2 days later for measurement of HTT levels. For detection of HTT-LC3 colocalization, the cells were fixed 4 h after compound treatment. For caspase-3 activation detection, the cells were stressed (BDNF removal for iPS-cell-derived neurons) 1 day after compound treatment, and tested at the indicated time points.

Chemical Information & Solubility

Molecular Weight 411.04 Formula

C15H9Br2NO3
CAS No. 486443-73-6 SDF --
Smiles C1=CC2=C(C=C1Br)C(=CC3=CC(=C(C(=C3)Br)O)O)C(=O)N2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 82 mg/mL ( (199.49 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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