Name: KB-R7943 mesylate
Brand: Selleck Chemicals
SKU: S4643
Rating: 5 (1 reviews)

KB-R7943 mesylate is a widely used inhibitor of the reverse Na+/Ca2+ exchanger (NCX(rev)) with IC50 of 5.7 μM. KB-R7943 mesylate promotes prostate cancer cell death by activating the JNK pathway and blocking autophagic flux.

KB-R7943 mesylate Chemical Structure

CAS: 182004-65-5

Purity & Quality Control

Batch: S464301 DMSO] 86 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.86%

99.86

KB-R7943 mesylate Related Products

Related Targets	NCX1 NCX2 NCX3	Click to Expand
Related Compound Libraries	FDA-approved Drug Library Natural Product Library Ion Channel Ligand Library Exosome Secretion Related Compound Library Calcium Channel Blocker Library	Click to Expand

Choose Selective NCX Inhibitors

Biological Activity

Description

KB-R7943 mesylate is a widely used inhibitor of the reverse Na+/Ca2+ exchanger (NCX(rev)) with IC50 of 5.7 μM. KB-R7943 mesylate promotes prostate cancer cell death by activating the JNK pathway and blocking autophagic flux.

Targets

NCX(rev) ^[1]
(Cell-free assay)

5.7 μM

In vitro
In vitro	KB-R7943 inhibits the reverse NCX more potently than its forward mode, thus occludes NCX-mediated Ca2+ extrusion and elevates intracellular Ca²⁺ level of melanoma.^[3]
Cell Research	Cell lines	Flua-4 AM loaded A2058 melanoma cells
	Concentrations	25 or 50 µM
	Incubation Time	5 min
	Method	Cells were plated in 96-well cell culture plates at 6000 per well and cultured overnight. Cells were treated with test reagents for 24–72 h according to different requirements. Cells were cultured in 96-well plates at 6000 per well and loaded with the cell membrane permeable Ca²⁺ dye Fluo-4 AM (5 μM) in HEPES-buffered solution for 40 min. The HEPES solution was supplemented with 1% FBS at pH 7.4.

References

Chemical Information & Solubility

Molecular Weight	427.5	Formula	C₁₇H₂₁N₃O₆S₂
CAS No.	182004-65-5	SDF	--
Smiles	C[S](O)(=O)=O.NC(=N)SCCC1=CC=C(OCC2=CC=C(C=C2)[N+]([O-])=O)C=C1
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 86 mg/mL ( (201.16 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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