Home Cell Cycle eIF inhibitor ISRIB For research use only.

ISRIB

ISRIB is an integrated stress response (ISR) inhibitor that potently reverses the effects of eukaryotic initiation factor 2α (eIF2α) phosphorylation.

ISRIB Chemical Structure

ISRIB Chemical Structure

CAS: 548470-11-7

Selleck's ISRIB has been cited by 16 publications

Purity & Quality Control

Batch: Purity: 98.81%
98.81

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Biological Activity

Description ISRIB is an integrated stress response (ISR) inhibitor that potently reverses the effects of eukaryotic initiation factor 2α (eIF2α) phosphorylation.
Targets
ISR [1] eIF2α [1]
In vitro
In vitro

ISRIB, an integrated stress response (ISR) inhibitor, reverses the effect of thapsigargin (TG) on translation, notably inhibits ER stress-induced cFLIP loss and prevents cFLIP levels down-regulation.[2]
Cell Research Cell lines HCT116 cells
Concentrations 220 nM
Incubation Time 1 h
Method

HCT116 cells are pre-treated with ISRIB (220 nM) for 1 h prior TG treatment for the indicated times. To analyze protein synthesis, puromycin is added in the last 10 min of treatment and puromycin incorporation to the nascent protein chain is assessed by western blotting using an anti-puromycin antibody. Levels of both cFLIP isoforms are determined in whole cell extracts by western blotting and quantified.
In Vivo
In vivo

In an instability-induced intervertebral disc degeneration (IDD) model in mice, ISRIB suppressed p-eIF2α/ATF4/Ihh and prevented IDD via protecting the anti-oxidative enzymes and decreased the NP cell apoptosis.[3]
Animal Research Animal Models C57 background BL/6J mice of intervertebral disc degeneration (IDD) models
Dosages 0.25 mg/kg
Administration s.c.

Chemical Information & Solubility

Molecular Weight 451.34 Formula
C22H24Cl2N2O4
CAS No. 548470-11-7 SDF --
Smiles ClC1=CC=C(OCC(=O)NC2CCC(CC2)NC(=O)COC3=CC=C(Cl)C=C3)C=C1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 10 mg/mL ( (22.15 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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