Salubrinal

For research use only.

Catalog No.S2923

15 publications

Salubrinal Chemical Structure

Molecular Weight(MW): 479.81

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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10mM (1mL in DMSO) USD 280 In stock
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Selleck's Salubrinal has been cited by 15 publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

  • PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell NWfJO2F6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGxRNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkO3NVEzKM7:TR?= MlvPV2FPT0WU
human 697 cell MofrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYTqToxrUW6qaXLpeIlwdiCxZjDoeY1idiB4OUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlk5Pzh6IN88US=> MoDWV2FPT0WU
human ALL-PO cell M2PE[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXqwXY17UW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01NjB6NkW1JO69VQ>? NWDVeoEyW0GQR1XS
human ML-2 cell NF;JRnJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NInPUZRKdmirYnn0bY9vKG:oIHj1cYFvKE2OLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlYyQDN|IN88US=> NGfJeFFUSU6JRWK=
human LB2241-RCC cell M{H3cmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXHJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01Njl6NUeyJO69VQ>? MorPV2FPT0WU
human Becker cell NILEepJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3PnVmlvcGmkaYTpc44hd2ZiaIXtZY4hSmWla3XyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44QTRyMTFOwG0> MmjhV2FPT0WU
human NEC8 cell MnTQS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWHhe49iUW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4xPTN|MTFOwG0> M1PpW3NCVkeHUh?=
human Daudi cell NIX5dmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXT5eGtrUW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvOzN4OUmg{txO Moj0V2FPT0WU
human MHH-PREB-1 cell MnO5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYHveFJPUW6qaXLpeIlwdiCxZjDoeY1idiCPSFitVHJGSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj63PFgyPyEQvF2= NGDGNnRUSU6JRWK=
human NCI-H510A cell MkLtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfIO4pKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IOVExSSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMEO1N|Uh|ryP NVnFXpl2W0GQR1XS
human NCI-H720 cell NXHZd2s6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV2wRpNIUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFczOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwNUeyOlgh|ryP M2\HbXNCVkeHUh?=
human MRK-nu-1 cell M1PSc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEPkOIpKdmirYnn0bY9vKG:oIHj1cYFvKE2USz3ueU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py55NUG3NUDPxE1? MlvZV2FPT0WU
human LB2518-MEL cell M3faNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYnJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjVzOD3NSWwh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04Njl5M{K2JO69VQ>? M4TxTXNCVkeHUh?=
human BE-13 cell NFLMbYpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIHCOVJKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE4zPjh2IN88US=> MXLTRW5ITVJ?
human A3-KAW cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmOzTY5pcWKrdHnvckBw\iCqdX3hckBCOy2NQWegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlQ{Ojh7IN88US=> MWDTRW5ITVJ?
human ST486 cell NEjt[FlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF\oR5FKdmirYnn0bY9vKG:oIHj1cYFvKFOWNEi2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42OzZ6IN88US=> M4fab3NCVkeHUh?=
human ES8 cell M2jaZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHTKVIlKdmirYnn0bY9vKG:oIHj1cYFvKEWVODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuOlQ{OzlizszN M13ubHNCVkeHUh?=
human HT cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWPJcohq[mm2aX;uJI9nKGi3bXHuJGhVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC57NEG4OUDPxE1? MY\TRW5ITVJ?
human HAL-01 cell M4PSbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NG\ib3RKdmirYnn0bY9vKG:oIHj1cYFvKEiDTD2wNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOzF3MEWg{txO MY\TRW5ITVJ?
human EB-3 cell NHLMR29Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2DBPGlvcGmkaYTpc44hd2ZiaIXtZY4hTUJvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuOlI2PzJizszN NFXGdmhUSU6JRWK=
human EW-1 cell MnGwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUXZZmhTUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU44OTJ2IN88US=> NYP5RoIxW0GQR1XS
human BL-41 cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYDjd29VUW6qaXLpeIlwdiCxZjDoeY1idiCETD20NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPzhyNEmg{txO NFzLUINUSU6JRWK=
Ramos-2G6-4C10 cell NVHsS2o3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWHFdINHUW6qaXLpeIlwdiCxZjDoeY1idiCUYX3vd{0zTzZvNFOxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvQTB|MUWg{txO NH\hfoNUSU6JRWK=
human DOHH-2 cell NYC1V2ZQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmnuTY5pcWKrdHnvckBw\iCqdX3hckBFV0iKLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4zOjZ{IN88US=> MVPTRW5ITVJ?
MONO-MAC-6 cell NEjXTmlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4\ZN2lvcGmkaYTpc44hd2ZiaIXtZY4hVU:QTz3NRWMuPiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyLkW0PFMh|ryP MkXzV2FPT0WU
human NKM-1 cell M2fSbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1jDZmlvcGmkaYTpc44hd2ZiaIXtZY4hVkuPLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE43OTl4IN88US=> M3PZWnNCVkeHUh?=
human HEL cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{\qRWlvcGmkaYTpc44hd2ZiaIXtZY4hUEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOlI2OiEQvF2= NX;UNIVyW0GQR1XS
human 8-MG-BA cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnzmTY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvQTl7OTFOwG0> MYfTRW5ITVJ?
human K-562 cell MlfoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUTtPIJEUW6qaXLpeIlwdiCxZjDoeY1idiCNLUW2NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjBzOUGg{txO M3SweHNCVkeHUh?=
human LXF-289 cell M3\idWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWroTFVwUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwMUW0O{DPxE1? NFn6TpJUSU6JRWK=
human HC-1 cell M4W3TGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MorkTY5pcWKrdHnvckBw\iCqdX3hckBJSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNVU1PyEQvF2= NXrmO5BFW0GQR1XS
human MC116 cell M2\2cGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUPJcohq[mm2aX;uJI9nKGi3bXHuJG1EOTF4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlA4OyEQvF2= MWPTRW5ITVJ?
human TE-12 cell MlnlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4HndWlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4{OzV5IN88US=> NUnNV4trW0GQR1XS
human BV-173 cell NF7TRm1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MorOTY5pcWKrdHnvckBw\iCqdX3hckBDXi1zN{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU42ODJzIN88US=> MYrTRW5ITVJ?
human NCCIT cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHjUbI5KdmirYnn0bY9vKG:oIHj1cYFvKE6FQ1nUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjB|OTFOwG0> MVfTRW5ITVJ?
human CESS cell NFvITYtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXfJcohq[mm2aX;uJI9nKGi3bXHuJGNGW1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT62OlYzKM7:TR?= NWjxXmw2W0GQR1XS
human HUTU-80 cell MlfHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{noTmlvcGmkaYTpc44hd2ZiaIXtZY4hUFWWVT24NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjZ5MESg{txO MUTTRW5ITVJ?
human IST-SL2 cell NITiZlRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHTxUm1KdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55N{C0JO69VQ>? NE\0c49USU6JRWK=
human ATN-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYP6OlJRUW6qaXLpeIlwdiCxZjDoeY1idiCDVF6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjB{Mk[g{txO NX;RenhmW0GQR1XS
human HCC1599 cell NIjjeYtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGfnfVBKdmirYnn0bY9vKG:oIHj1cYFvKEiFQ{G1PVkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOi5zMEm0JO69VQ>? NWXQW4p5W0GQR1XS
human NTERA-S-cl-D1 cell M1LJSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{LXW2lvcGmkaYTpc44hd2ZiaIXtZY4hVlSHUlGtV{1kdC2GMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlg5QDhizszN NFWze5ZUSU6JRWK=
human EVSA-T cell NXfJW3pKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVPJcohq[mm2aX;uJI9nKGi3bXHuJGVXW0FvVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlkzODRizszN NHTXSItUSU6JRWK=
human NALM-6 cell MofaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlnyTY5pcWKrdHnvckBw\iCqdX3hckBPSUyPLU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfU4hUUN3ME2xN{4xQTJ7IN88US=> M4q3bXNCVkeHUh?=
human KMOE-2 cell NWHsTlY6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWfubpJUUW6qaXLpeIlwdiCxZjDoeY1idiCNTV;FMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy5zMEmyJO69VQ>? M1LFN3NCVkeHUh?=
human HL-60 cell NH7YVGdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmHzTY5pcWKrdHnvckBw\iCqdX3hckBJVC14MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlM{ODhizszN M3TQdnNCVkeHUh?=
human D-542MG cell NHS3[XlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{fPcGlvcGmkaYTpc44hd2ZiaIXtZY4hTC13NELNS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjR|ME[g{txO M2TCeHNCVkeHUh?=
human L-363 cell NWTudpZzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXrJcohq[mm2aX;uJI9nKGi3bXHuJGwuOzZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOVU5OyEQvF2= NHKwZ4ZUSU6JRWK=
human CMK cell NUK0Uo5sT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXTJcohq[mm2aX;uJI9nKGi3bXHuJGNOUyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF|Lke0N|Yh|ryP M2DycHNCVkeHUh?=
human IMR-5 cell M2nMNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUjhdpJDUW6qaXLpeIlwdiCxZjDoeY1idiCLTWKtOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njd6NEmg{txO MmXwV2FPT0WU
human A101D cell MkO3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3LycmlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{45OjB5IN88US=> MVfTRW5ITVJ?
human MOLT-16 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PjZ3IN88US=> MlfFV2FPT0WU
human LU-139 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYPJcohq[mm2aX;uJI9nKGi3bXHuJGxWNTF|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0MlA1PDRizszN NFzSU3NUSU6JRWK=
human OVCAR-4 cell M1zwZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXfJcohq[mm2aX;uJI9nKGi3bXHuJG9XS0GULUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOE44PjB2IN88US=> MkOyV2FPT0WU
human BC-3 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVfJcohq[mm2aX;uJI9nKGi3bXHuJGJENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zND63PVgzKM7:TR?= MV\TRW5ITVJ?
PC12 cells M{nCemZ2dmO2aX;uJIF{e2G7 Ml63R5l1d3C{b4TlZ5RqfmViYXP0bZZqfHlib36gdoF1KHS3bnnjZY16[2mwIITy[YF1\WRicnH0JJBp\W:laILvcY9kgXSxbXGgZ4VtdCCuaX7lJHBEOTJiZoLvcUBieG:ydH;zbZMhcW6mdXPl[EBjgSCHbnTvdIxie22rYzDy[ZRq[3WudX2gd5Rz\XO|LDDFR|UxRTF3IN88US=> Mm\RNVYxODJ{OEi=
human D-336MG cell NWrEcoJJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2nBZWlvcGmkaYTpc44hd2ZiaIXtZY4hTC1|M{\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE2NjFzM{Sg{txO NGKxWXhUSU6JRWK=
human BC-1 cell NX[5PZlmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkPQTY5pcWKrdHnvckBw\iCqdX3hckBDSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNVk5KM7:TR?= NHrsN3hUSU6JRWK=
human KE-37 cell MoTsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVTIXZFPUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE2NjJ6MkSg{txO MVzTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
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  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A
Smiles ClC(Cl)(Cl)C(NC(=O)\C=C\C1=CC=CC=C1)NC(=S)NC2=CC=CC3=C2N=CC=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID