Salubrinal

Catalog No.S2923

Salubrinal Chemical Structure

Molecular Weight(MW): 479.81

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

Size Price Stock Quantity  
In DMSO USD 280 In stock
USD 147 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 9 Publications

3 Customer Reviews

  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

    PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell MmnHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXTxcWZQUW6qaXLpeIlwdiCxZjDoeY1idiCOUD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4{PzFzMjFOwG0> NX34do1wW0GQR1XS
human 697 cell NF\VS29Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnG4TY5pcWKrdHnvckBw\iCqdX3hckA3QTdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lkm4O|g5KM7:TR?= MUDTRW5ITVJ?
human ALL-PO cell MmHDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGjU[mZKdmirYnn0bY9vKG:oIHj1cYFvKEGOTD3QU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvODh4NUWg{txO NUWxS3FwW0GQR1XS
human ML-2 cell MkfES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUD4OFJvUW6qaXLpeIlwdiCxZjDoeY1idiCPTD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE43OTh|MzFOwG0> MlHCV2FPT0WU
human LB2241-RCC cell NYTSfHFzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn\lTY5pcWKrdHnvckBw\iCqdX3hckBNSjJ{NEGtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC57OEW3NkDPxE1? NFXafYxUSU6JRWK=
human Becker cell M4\vZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4PHTWlvcGmkaYTpc44hd2ZiaIXtZY4hSmWla3XyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU44QTRyMTFOwG0> NEfpToRUSU6JRWK=
human NEC8 cell MmjjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI\2fmxKdmirYnn0bY9vKG:oIHj1cYFvKE6HQ{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2OzNzIN88US=> M3S3[XNCVkeHUh?=
human Daudi cell NWXjO3ROT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGRifWSrIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6zN|Y6QSEQvF2= MWfTRW5ITVJ?
human MHH-PREB-1 cell NFrlcpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHjNXlRKdmirYnn0bY9vKG:oIHj1cYFvKE2KSD3QVmVDNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lke4PFE4KM7:TR?= NIHHXGRUSU6JRWK=
human NCI-H510A cell NIHKfWlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3SwemlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi1NVBCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yM{WzOUDPxE1? MoDrV2FPT0WU
human NCI-H720 cell NHT0N2FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXhTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEeyNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPTd{Nkig{txO MofrV2FPT0WU
human MRK-nu-1 cell NE\zXVJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1r6PWlvcGmkaYTpc44hd2ZiaIXtZY4hVVKNLX71MVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04Njd3MUexJO69VQ>? NYPVbGM5W0GQR1XS
human LB2518-MEL cell NX;l[JB1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4npPWlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{NUG4MW1GVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwOUezNlYh|ryP NGjj[JpUSU6JRWK=
human BE-13 cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV34eGVkUW6qaXLpeIlwdiCxZjDoeY1idiCERT2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjZ6NDFOwG0> MXnTRW5ITVJ?
human A3-KAW cell MnPHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWTJcohq[mm2aX;uJI9nKGi3bXHuJGE{NUuDVzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuOFMzQDlizszN MlnCV2FPT0WU
human ST486 cell NUWxNGxmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXHJcohq[mm2aX;uJI9nKGi3bXHuJHNVPDh4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD61N|Y5KM7:TR?= M{jNeXNCVkeHUh?=
human ES8 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYPoSZFIUW6qaXLpeIlwdiCxZjDoeY1idiCHU{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY1OzN7IN88US=> NXHTS2ZFW0GQR1XS
human HT cell M16zUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1uwXGlvcGmkaYTpc44hd2ZiaIXtZY4hUFRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lkm0NVg2KM7:TR?= NHvVcVZUSU6JRWK=
human HAL-01 cell NUHLUVdRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXizSGdmUW6qaXLpeIlwdiCxZjDoeY1idiCKQVytNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjNzNUC1JO69VQ>? NX7ITFZkW0GQR1XS
human EB-3 cell NHfMSGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkC1TY5pcWKrdHnvckBw\iCqdX3hckBGSi1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT62NlU4OiEQvF2= NVXh[4tqW0GQR1XS
human EW-1 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{e1ZmlvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|EzPCEQvF2= MXjTRW5ITVJ?
human BL-41 cell NUTq[WxMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGHGeYlKdmirYnn0bY9vKG:oIHj1cYFvKEKOLUSxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU44QDB2OTFOwG0> M3zCdXNCVkeHUh?=
Ramos-2G6-4C10 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWPlR2w5UW6qaXLpeIlwdiCxZjDoeY1idiCUYX3vd{0zTzZvNFOxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvQTB|MUWg{txO M2TlbXNCVkeHUh?=
human DOHH-2 cell MnXRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXLJcohq[mm2aX;uJI9nKGi3bXHuJGRQUEhvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlIzPjJizszN NETvZ|VUSU6JRWK=
MONO-MAC-6 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NY\BN4l{UW6qaXLpeIlwdiCxZjDoeY1idiCPT17PMW1CSy14IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOVQ5OyEQvF2= NXrMbJJDW0GQR1XS
human NKM-1 cell MmXLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWj4c5QyUW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjZzOU[g{txO NVTTT|BDW0GQR1XS
human HEL cell MnnUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M13ROmlvcGmkaYTpc44hd2ZiaIXtZY4hUEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOlI2OiEQvF2= NGnES3lUSU6JRWK=
human 8-MG-BA cell MnT3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmXITY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvQTl7OTFOwG0> Ml7rV2FPT0WU
human K-562 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWLJcohq[mm2aX;uJI9nKGi3bXHuJGsuPTZ{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNFE6OSEQvF2= MX\TRW5ITVJ?
human LXF-289 cell NVjNUlZVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3vUOGlvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjF3NEeg{txO NULmfGRMW0GQR1XS
human HC-1 cell NVzpbnJ4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3\mbGlvcGmkaYTpc44hd2ZiaIXtZY4hUENvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlE2PDdizszN M3fzVnNCVkeHUh?=
human MC116 cell NF7H[XRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEHuRm9KdmirYnn0bY9vKG:oIHj1cYFvKE2FMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB5MzFOwG0> NYf6UWlSW0GQR1XS
human TE-12 cell NVvOTFhsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVPCZolPUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjN|NUeg{txO M1m2VnNCVkeHUh?=
human BV-173 cell M37OVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWHMWoc2UW6qaXLpeIlwdiCxZjDoeY1idiCEVj2xO|Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS53MEKxJO69VQ>? MlvEV2FPT0WU
human NCCIT cell MknaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlruTY5pcWKrdHnvckBw\iCqdX3hckBPS0OLVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlYxOzlizszN NGGySlNUSU6JRWK=
human CESS cell NHrHSWFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX7CXVBmUW6qaXLpeIlwdiCxZjDoeY1idiCFRWPTJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjZ4MjFOwG0> NFPhNpdUSU6JRWK=
human HUTU-80 cell NWDxPVZQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{XRWWlvcGmkaYTpc44hd2ZiaIXtZY4hUFWWVT24NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjZ5MESg{txO M4rRb3NCVkeHUh?=
human IST-SL2 cell NGO1UGdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXjJclhIUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wzKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwN{ewOEDPxE1? NVfzN|VbW0GQR1XS
human ATN-1 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NULFNpc5UW6qaXLpeIlwdiCxZjDoeY1idiCDVF6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjB{Mk[g{txO M3r0eXNCVkeHUh?=
human HCC1599 cell MnrtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUjIRYVEUW6qaXLpeIlwdiCxZjDoeY1idiCKQ1OxOVk6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTJwMUC5OEDPxE1? MmPKV2FPT0WU
human NTERA-S-cl-D1 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXT6d3NUUW6qaXLpeIlwdiCxZjDoeY1idiCQVFXSRU1UNWOuLVSxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvQDh6ODFOwG0> MXfTRW5ITVJ?
human EVSA-T cell MonJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2fCOmlvcGmkaYTpc44hd2ZiaIXtZY4hTV[VQT3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvQTJyNDFOwG0> NV3BU4tVW0GQR1XS
human NALM-6 cell NVfHcIdmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4iyXmlvcGmkaYTpc44hd2ZiaIXtZY4hVkGOTT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZkvKEmFNUC9NVMvODl{OTFOwG0> Ml3ZV2FPT0WU
human KMOE-2 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGe5U2JKdmirYnn0bY9vKG:oIHj1cYFvKEuPT1WtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjFyOUKg{txO MoLwV2FPT0WU
human HL-60 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXu2WZZ7UW6qaXLpeIlwdiCxZjDoeY1idiCKTD22NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjN|MEig{txO MlvFV2FPT0WU
human D-542MG cell M3:5SGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWLyVYtyUW6qaXLpeIlwdiCxZjDoeY1idiCGLUW0Nm1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwNEOwOkDPxE1? NHLDeHBUSU6JRWK=
human L-363 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2LJWmlvcGmkaYTpc44hd2ZiaIXtZY4hVC1|NkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{42PTh|IN88US=> Mn7QV2FPT0WU
human CMK cell M1m1N2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWXrNnBYUW6qaXLpeIlwdiCxZjDoeY1idiCFTVugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{44PDN4IN88US=> MnfMV2FPT0WU
human IMR-5 cell NFiyZZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVvGTlRqUW6qaXLpeIlwdiCxZjDoeY1idiCLTWKtOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njd6NEmg{txO Mn;XV2FPT0WU
human A101D cell NYr5[VV6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M13qXmlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{45OjB5IN88US=> MXPTRW5ITVJ?
human MOLT-16 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVXJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PjZ3IN88US=> NXXZeIhbW0GQR1XS
human LU-139 cell M3mwbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{fUVmlvcGmkaYTpc44hd2ZiaIXtZY4hVFVvMUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvODR2NDFOwG0> M1ftSXNCVkeHUh?=
human OVCAR-4 cell NEL5W4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2f6OmlvcGmkaYTpc44hd2ZiaIXtZY4hV1[FQWKtOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE1Njd4MESg{txO MlXLV2FPT0WU
human BC-3 cell NHTLVG5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MULJcohq[mm2aX;uJI9nKGi3bXHuJGJENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zND63PVgzKM7:TR?= NXzXV|FVW0GQR1XS
PC12 cells NGHMRnJHfW6ldHnvckBie3OjeR?= MX3DfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCxbjDyZZQhfHWwaXPhcZlkcW5idILlZZRm\CC{YYSgdIhmd2Oqcn;tc4N6fG:vYTDj[YxtKGyrbnWgVGMyOiCocn;tJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEWwZH;wcIF{dWmlIILleIlkfWy3bTDzeJJme3NuIFXDOVA:OTVizszN NEP3eYMyPjByMkK4PC=>
human D-336MG cell MkSxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml3NTY5pcWKrdHnvckBw\iCqdX3hckBFNTN|Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOTF|NDFOwG0> MojXV2FPT0WU
human BC-1 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWjOUnZqUW6qaXLpeIlwdiCxZjDoeY1idiCEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOTl6IN88US=> Ml3DV2FPT0WU
human KE-37 cell MmG3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2m3PWlvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4zQDJ2IN88US=> MlrxV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
- Collapse
  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
- Collapse
  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Formulation: in DMEM
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Salubrinal | Salubrinal supplier | purchase Salubrinal | Salubrinal cost | Salubrinal manufacturer | order Salubrinal | Salubrinal distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID