Salubrinal

For research use only.

Catalog No.S2923

15 publications

Salubrinal Chemical Structure

CAS No. 405060-95-9

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 280 In stock
USD 147 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Salubrinal has been cited by 15 publications

3 Customer Reviews

  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

  • PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell M1:1WGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEHnT|dKdmirYnn0bY9vKG:oIHj1cYFvKEySLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlM4OTF{IN88US=> MnvIV2FPT0WU
human 697 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3z5VGlvcGmkaYTpc44hd2ZiaIXtZY4hPjl5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mz65PFc5QCEQvF2= M3fvVXNCVkeHUh?=
human ALL-PO cell NVLDRYNLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWWwb4RKUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01NjB6NkW1JO69VQ>? MUDTRW5ITVJ?
human ML-2 cell NGXtcWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWjJcohq[mm2aX;uJI9nKGi3bXHuJG1NNTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lk[xPFM{KM7:TR?= MoDjV2FPT0WU
human LB2241-RCC cell MnPtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2\n[2lvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{MkSxMXJESyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwOUi1O|Ih|ryP MXrTRW5ITVJ?
human Becker cell Mlz1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGJm[2uncjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuO|k1ODFizszN MlLXV2FPT0WU
human NEC8 cell NWXzUJliT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkC1N|MyKM7:TR?= NEnTfHBUSU6JRWK=
human Daudi cell M4LLeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGRifWSrIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6zN|Y6QSEQvF2= MkLsV2FPT0WU
human MHH-PREB-1 cell NVjsW3ZkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkfyTY5pcWKrdHnvckBw\iCqdX3hckBOUEhvUGLFRk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi55OEixO{DPxE1? MkPTV2FPT0WU
human NCI-H510A cell NF\sb4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVTJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNUGwRUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvODN3M{Wg{txO NGjTfWlUSU6JRWK=
human NCI-H720 cell NXTkdJVFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2fEXGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjV5Mk[4JO69VQ>? MoP2V2FPT0WU
human MRK-nu-1 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGLW[INKdmirYnn0bY9vKG:oIHj1cYFvKE2USz3ueU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py55NUG3NUDPxE1? MXzTRW5ITVJ?
human LB2518-MEL cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mmm0TY5pcWKrdHnvckBw\iCqdX3hckBNSjJ3MUitUWVNKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py57N{OyOkDPxE1? NWGyem1RW0GQR1XS
human BE-13 cell NIm1V3FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\TTY5pcWKrdHnvckBw\iCqdX3hckBDTS1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlY5PCEQvF2= MknaV2FPT0WU
human A3-KAW cell MnjHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV6ye4ZTUW6qaXLpeIlwdiCxZjDoeY1idiCDMz3LRXch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjR|Mki5JO69VQ>? MV\TRW5ITVJ?
human ST486 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{DGdGlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlU{PjhizszN NYS3WGVEW0GQR1XS
human ES8 cell MknDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3fVXmlvcGmkaYTpc44hd2ZiaIXtZY4hTVN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD62OFM{QSEQvF2= MYrTRW5ITVJ?
human HT cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWrJcohq[mm2aX;uJI9nKGi3bXHuJGhVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC57NEG4OUDPxE1? NVi4[|hHW0GQR1XS
human HAL-01 cell NV6ySG5pT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGhCVC1yMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuN|E2ODVizszN MlrVV2FPT0WU
human EB-3 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NITVfIFKdmirYnn0bY9vKG:oIHj1cYFvKEWELUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlYzPTd{IN88US=> Moi5V2FPT0WU
human EW-1 cell NH;uTlBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1LRT2lvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|EzPCEQvF2= NV\TXlVCW0GQR1XS
human BL-41 cell NV3zZmJ1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoLxTY5pcWKrdHnvckBw\iCqdX3hckBDVC12MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|gxPDlizszN MYnTRW5ITVJ?
Ramos-2G6-4C10 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHLT[IlKdmirYnn0bY9vKG:oIHj1cYFvKFKjbX;zMVJIPi12Q{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU46ODNzNTFOwG0> M1exVXNCVkeHUh?=
human DOHH-2 cell NHjHO2ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\WTY5pcWKrdHnvckBw\iCqdX3hckBFV0iKLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4zOjZ{IN88US=> MX3TRW5ITVJ?
MONO-MAC-6 cell M3T0VGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXvJcohq[mm2aX;uJI9nKGi3bXHuJG1QVk9vTVHDMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC53NEizJO69VQ>? MlXTV2FPT0WU
human NKM-1 cell MkLVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYjJcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOlE6PiEQvF2= NFz5cplUSU6JRWK=
human HEL cell NHzBfZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGnXO4FKdmirYnn0bY9vKG:oIHj1cYFvKEiHTDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlYzPTJizszN MmPTV2FPT0WU
human 8-MG-BA cell M3\kRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnXZTY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvQTl7OTFOwG0> NYTzc45TW0GQR1XS
human K-562 cell NYHtWnI5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVTJcohq[mm2aX;uJI9nKGi3bXHuJGsuPTZ{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNFE6OSEQvF2= MYXTRW5ITVJ?
human LXF-289 cell NIrxSGxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yPTR5IN88US=> MofXV2FPT0WU
human HC-1 cell MnPxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3fuN2lvcGmkaYTpc44hd2ZiaIXtZY4hUENvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlE2PDdizszN MXPTRW5ITVJ?
human MC116 cell NIO5OZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGm0d29KdmirYnn0bY9vKG:oIHj1cYFvKE2FMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjB5MzFOwG0> MVPTRW5ITVJ?
human TE-12 cell NFHHVpJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYTjcmp5UW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjN|NUeg{txO NIi1cIxUSU6JRWK=
human BV-173 cell M3LkeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEXmS3pKdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjVyMkGg{txO MVzTRW5ITVJ?
human NCCIT cell M3fIUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXXURYJYUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1PJWEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjZyM{mg{txO NF3UWphUSU6JRWK=
human CESS cell NF;pV2VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYLJcohq[mm2aX;uJI9nKGi3bXHuJGNGW1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT62OlYzKM7:TR?= MmPEV2FPT0WU
human HUTU-80 cell NV[2PXhHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWHZPGFsUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwNkewOEDPxE1? M1i0OHNCVkeHUh?=
human IST-SL2 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoTUTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPzdyNDFOwG0> M3rSVHNCVkeHUh?=
human ATN-1 cell NVvrfIt1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUK2e4o{UW6qaXLpeIlwdiCxZjDoeY1idiCDVF6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjB{Mk[g{txO NWXYNlhPW0GQR1XS
human HCC1599 cell NVmzbWxWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3K4cGlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFMUW5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjFyOUSg{txO NWrPXW15W0GQR1XS
human NTERA-S-cl-D1 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn\vTY5pcWKrdHnvckBw\iCqdX3hckBPXEWUQT3TMYNtNURzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUKuPFg5QCEQvF2= MoexV2FPT0WU
human EVSA-T cell MorIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIrMPIVKdmirYnn0bY9vKG:oIHj1cYFvKEWYU1GtWEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjl{MESg{txO MUTTRW5ITVJ?
human NALM-6 cell NF24e4lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MofYTY5pcWKrdHnvckBw\iCqdX3hckBPSUyPLU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfU4hUUN3ME2xN{4xQTJ7IN88US=> NHXhNmVUSU6JRWK=
human KMOE-2 cell NWrZVVZbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmG1TY5pcWKrdHnvckBw\iCqdX3hckBMVU:HLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4yODl{IN88US=> NX3QS2I5W0GQR1XS
human HL-60 cell NUT0SHJJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXTDbZVqUW6qaXLpeIlwdiCxZjDoeY1idiCKTD22NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjN|MEig{txO M4n0XHNCVkeHUh?=
human D-542MG cell NEXIZVJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlToTY5pcWKrdHnvckBw\iCqdX3hckBFNTV2Ml3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPDNyNjFOwG0> NV;EdWVmW0GQR1XS
human L-363 cell MmTFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2rBR2lvcGmkaYTpc44hd2ZiaIXtZY4hVC1|NkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{42PTh|IN88US=> NWntRXNsW0GQR1XS
human CMK cell M1\wT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUDJcohq[mm2aX;uJI9nKGi3bXHuJGNOUyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF|Lke0N|Yh|ryP MmjsV2FPT0WU
human IMR-5 cell M3Hjc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2DZZWlvcGmkaYTpc44hd2ZiaIXtZY4hUU2ULUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{44QDR7IN88US=> MXTTRW5ITVJ?
human A101D cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmjzTY5pcWKrdHnvckBw\iCqdX3hckBCOTBzRDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlgzODdizszN Mnv0V2FPT0WU
human MOLT-16 cell NI\YPG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PjZ3IN88US=> MUPTRW5ITVJ?
human LU-139 cell M2TuVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXfOeottUW6qaXLpeIlwdiCxZjDoeY1idiCOVT2xN|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPC5yNES0JO69VQ>? MnrhV2FPT0WU
human OVCAR-4 cell NXTaSXdyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHnxNHRKdmirYnn0bY9vKG:oIHj1cYFvKE:YQ1HSMVQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPC55NkC0JO69VQ>? NFXkV3ZUSU6JRWK=
human BC-3 cell NH60RoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1;PSmlvcGmkaYTpc44hd2ZiaIXtZY4hSkNvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0Mlc6QDJizszN MWHTRW5ITVJ?
PC12 cells MnXISpVv[3Srb36gZZN{[Xl? M4[yNWN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IH;uJJJifCC2dX7pZ4FugWOrbjD0doVifGWmIILheEBxcGWxY3jyc41w[3m2b33hJINmdGxibHnu[UBRSzF{IH\yc40h[XCxcITvd4l{KGmwZIXj[YQh[nliRX7kc5Bt[XOvaXOgdoV1cWO3bIXtJJN1emW|czygSWM2OD1zNTFOwG0> NV\WfYhvOTZyMEKyPFg>
human D-336MG cell NH\CT4JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NETLNWVKdmirYnn0bY9vKG:oIHj1cYFvKERvM{O2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS5zMUO0JO69VQ>? M3LIcXNCVkeHUh?=
human BC-1 cell NXu5VYs2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFrWd|RKdmirYnn0bY9vKG:oIHj1cYFvKEKFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4yQThizszN NWjHSZVEW0GQR1XS
human KE-37 cell NYfYO3UxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoO3TY5pcWKrdHnvckBw\iCqdX3hckBMTS1|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG1MlI5OjRizszN M2rkW3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
- Collapse
  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
- Collapse
  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C=C1)C=CC(=O)NC(C(Cl)(Cl)Cl)NC(=S)NC2=CC=CC3=C2N=CC=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Salubrinal|Salubrinal ic50|Salubrinal price|Salubrinal cost|Salubrinal solubility dmso|Salubrinal purchase|Salubrinal manufacturer|Salubrinal research buy|Salubrinal order|Salubrinal mouse|Salubrinal chemical structure|Salubrinal mw|Salubrinal molecular weight|Salubrinal datasheet|Salubrinal supplier|Salubrinal in vitro|Salubrinal cell line|Salubrinal concentration|Salubrinal nmr|Salubrinal in vivo|Salubrinal clinical trial|Salubrinal inhibitor|Salubrinal Apoptosis inhibitor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID