Salubrinal

For research use only. Not for use in humans.

Catalog No.S2923

10 publications

Salubrinal Chemical Structure

Molecular Weight(MW): 479.81

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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10mM (1mL in DMSO) USD 280 In stock
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Selleck's Salubrinal has been cited by 10 publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

    PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXm4TmZNUW6qaXLpeIlwdiCxZjDoeY1idiCOUD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4{PzFzMjFOwG0> NFLyNGRUSU6JRWK=
human 697 cell M3TQfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGDGXFBKdmirYnn0bY9vKG:oIHj1cYFvKDZ7NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuPVg4QDhizszN NXrk[GZYW0GQR1XS
human ALL-PO cell NImzZ2pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1jhcmlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4xQDZ3NTFOwG0> NHf1PWVUSU6JRWK=
human ML-2 cell Mn65S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoGwTY5pcWKrdHnvckBw\iCqdX3hckBOVC1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND62NVg{OyEQvF2= MXTTRW5ITVJ?
human LB2241-RCC cell NUHhXJVHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUC0NZl2UW6qaXLpeIlwdiCxZjDoeY1idiCOQkKyOFEuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND65PFU4OiEQvF2= NXf0SGczW0GQR1XS
human Becker cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYHzdmR2UW6qaXLpeIlwdiCxZjDoeY1idiCEZXPr[ZIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd7NECxJO69VQ>? M4rnOHNCVkeHUh?=
human NEC8 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGLCU|dKdmirYnn0bY9vKG:oIHj1cYFvKE6HQ{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2OzNzIN88US=> MXXTRW5ITVJ?
human Daudi cell MnHsS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUjnemw{UW6qaXLpeIlwdiCxZjDoeY1idiCGYYXkbUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvOzN4OUmg{txO NU[3SGtyW0GQR1XS
human MHH-PREB-1 cell NF;IT4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXy3emVDUW6qaXLpeIlwdiCxZjDoeY1idiCPSFitVHJGSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj63PFgyPyEQvF2= NXT5RlQ1W0GQR1XS
human NCI-H510A cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWqwRlVWUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFUyOEFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15LkCzOVM2KM7:TR?= MYTTRW5ITVJ?
human NCI-H720 cell NVHkcVFKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4jHXmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjV5Mk[4JO69VQ>? NIrRUFBUSU6JRWK=
human MRK-nu-1 cell NF\0OphIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmfMTY5pcWKrdHnvckBw\iCqdX3hckBOWktvboWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPzVzN{Gg{txO MWTTRW5ITVJ?
human LB2518-MEL cell NF;KPXRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXnJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjVzOD3NSWwh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04Njl5M{K2JO69VQ>? M4fnPHNCVkeHUh?=
human BE-13 cell NHj4NFNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIPwbWlKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE4zPjh2IN88US=> Mo\KV2FPT0WU
human A3-KAW cell Mn;pS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWLJcohq[mm2aX;uJI9nKGi3bXHuJGE{NUuDVzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuOFMzQDlizszN NG\Iem5USU6JRWK=
human ST486 cell NHuwXJFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYfJcohq[mm2aX;uJI9nKGi3bXHuJHNVPDh4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD61N|Y5KM7:TR?= NVWzSpAyW0GQR1XS
human ES8 cell NIjRTotIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnjITY5pcWKrdHnvckBw\iCqdX3hckBGWzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lk[0N|M6KM7:TR?= NGHjRmhUSU6JRWK=
human HT cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUjJcohq[mm2aX;uJI9nKGi3bXHuJGhVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC57NEG4OUDPxE1? NYHEcHpVW0GQR1XS
human HAL-01 cell MoLHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYrJcohq[mm2aX;uJI9nKGi3bXHuJGhCVC1yMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuN|E2ODVizszN NWn4PFFuW0GQR1XS
human EB-3 cell NHm1eXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M37RPWlvcGmkaYTpc44hd2ZiaIXtZY4hTUJvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuOlI2PzJizszN NFj6[HNUSU6JRWK=
human EW-1 cell NVHSVplVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1jBSGlvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|EzPCEQvF2= NHK5dVZUSU6JRWK=
human BL-41 cell NHGzN2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mk\DTY5pcWKrdHnvckBw\iCqdX3hckBDVC12MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|gxPDlizszN Mli3V2FPT0WU
Ramos-2G6-4C10 cell NYDUNpVPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVHJcohq[mm2aX;uJI9nKGi3bXHuJHJidW:|LULHOk01SzFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT65NFMyPSEQvF2= M1zCO3NCVkeHUh?=
human DOHH-2 cell M1;i[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4PUW2lvcGmkaYTpc44hd2ZiaIXtZY4hTE:KSD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOjJ4MjFOwG0> NUPLZ4J5W0GQR1XS
MONO-MAC-6 cell NUT4NVZjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVrJcohq[mm2aX;uJI9nKGi3bXHuJG1QVk9vTVHDMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC53NEizJO69VQ>? MlrPV2FPT0WU
human NKM-1 cell NX7rXZBRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVjJcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOlE6PiEQvF2= NV;1b3U6W0GQR1XS
human HEL cell Ml;lS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlnHTY5pcWKrdHnvckBw\iCqdX3hckBJTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMD62NlUzKM7:TR?= MVfTRW5ITVJ?
human 8-MG-BA cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUK0NHVFUW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwOUm5PUDPxE1? NXvMOZhNW0GQR1XS
human K-562 cell M3u5[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{HTVmlvcGmkaYTpc44hd2ZiaIXtZY4hUy13NkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4xOTlzIN88US=> NWPnXZRHW0GQR1XS
human LXF-289 cell NXnwZYR6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml3MTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTV2NzFOwG0> MUDTRW5ITVJ?
human HC-1 cell M2PxWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NG\YXGVKdmirYnn0bY9vKG:oIHj1cYFvKEiFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yPTR5IN88US=> NWfBO2hUW0GQR1XS
human MC116 cell Ml;qS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXXJcohq[mm2aX;uJI9nKGi3bXHuJG1EOTF4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlA4OyEQvF2= MYfTRW5ITVJ?
human TE-12 cell NVnWdHVKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYfJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuN|M2PyEQvF2= NYKxXJFWW0GQR1XS
human BV-173 cell M2LXbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGJXNTF5MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlUxOjFizszN NWjU[ng2W0GQR1XS
human NCCIT cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4HNbmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOFSWSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU43ODN7IN88US=> MXvTRW5ITVJ?
human CESS cell NVLhSY5[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGNGW1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT62OlYzKM7:TR?= MX3TRW5ITVJ?
human HUTU-80 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWm1UoZHUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwNkewOEDPxE1? MY\TRW5ITVJ?
human IST-SL2 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXnJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU44PzB2IN88US=> MUDTRW5ITVJ?
human ATN-1 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NELVbItKdmirYnn0bY9vKG:oIHj1cYFvKEGWTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvODJ{NjFOwG0> MX7TRW5ITVJ?
human HCC1599 cell Mnj5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYe4UVlHUW6qaXLpeIlwdiCxZjDoeY1idiCKQ1OxOVk6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTJwMUC5OEDPxE1? NEP2SYdUSU6JRWK=
human NTERA-S-cl-D1 cell NUHtUGE{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2jzRWlvcGmkaYTpc44hd2ZiaIXtZY4hVlSHUlGtV{1kdC2GMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlg5QDhizszN MUXTRW5ITVJ?
human EVSA-T cell M33WeWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{LISWlvcGmkaYTpc44hd2ZiaIXtZY4hTV[VQT3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvQTJyNDFOwG0> NXjne4J5W0GQR1XS
human NALM-6 cell MkHjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJG5CVE1vNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MkBKSzVyPUGzMlA6OjlizszN MmjzV2FPT0WU
human KMOE-2 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKGi3bXHuJGtOV0VvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlExQTJizszN MXvTRW5ITVJ?
human HL-60 cell M4[y[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1Tu[mlvcGmkaYTpc44hd2ZiaIXtZY4hUExvNkCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4{OzB6IN88US=> MVzTRW5ITVJ?
human D-542MG cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTR{TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{41OzB4IN88US=> MXrTRW5ITVJ?
human L-363 cell NXXne|hbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFTWeHhKdmirYnn0bY9vKG:oIHj1cYFvKExvM{[zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPTV6MzFOwG0> M37jWXNCVkeHUh?=
human CMK cell MnjpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4ixXmlvcGmkaYTpc44hd2ZiaIXtZY4hS02NIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuO|Q{PiEQvF2= MVfTRW5ITVJ?
human IMR-5 cell NH;SelRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGHKRY5KdmirYnn0bY9vKG:oIHj1cYFvKEmPUj21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPzh2OTFOwG0> NVXMU2k4W0GQR1XS
human A101D cell MkniS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnywTY5pcWKrdHnvckBw\iCqdX3hckBCOTBzRDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlgzODdizszN Mmi4V2FPT0WU
human MOLT-16 cell NWn6bGg5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYnvUlFEUW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVE3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwOU[2OUDPxE1? NGHsNWFUSU6JRWK=
human LU-139 cell NW\FdFFyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV3MSFRxUW6qaXLpeIlwdiCxZjDoeY1idiCOVT2xN|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPC5yNES0JO69VQ>? M4jEZ3NCVkeHUh?=
human OVCAR-4 cell NWjrPWJkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mk\mTY5pcWKrdHnvckBw\iCqdX3hckBQXkODUj20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvPzZyNDFOwG0> M{\WNXNCVkeHUh?=
human BC-3 cell M3PzTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2rib2lvcGmkaYTpc44hd2ZiaIXtZY4hSkNvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0Mlc6QDJizszN NFjXWFhUSU6JRWK=
PC12 cells MYDGeY5kfGmxbjDhd5NigQ>? M37lR2N6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IH;uJJJifCC2dX7pZ4FugWOrbjD0doVifGWmIILheEBxcGWxY3jyc41w[3m2b33hJINmdGxibHnu[UBRSzF{IH\yc40h[XCxcITvd4l{KGmwZIXj[YQh[nliRX7kc5Bt[XOvaXOgdoV1cWO3bIXtJJN1emW|czygSWM2OD1zNTFOwG0> M2XUUVE3ODB{Mki4
human D-336MG cell NWLLeIFST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUXJcohq[mm2aX;uJI9nKGi3bXHuJGQuOzN4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4yOTN2IN88US=> MVnTRW5ITVJ?
human BC-1 cell Ml3OS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlPITY5pcWKrdHnvckBw\iCqdX3hckBDSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNVk5KM7:TR?= M1zTfnNCVkeHUh?=
human KE-37 cell NXn4OIxCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXu4eYJMUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE2NjJ6MkSg{txO NXT5foZMW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
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  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Formulation: in DMEM
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A
Smiles ClC(Cl)(Cl)C(NC(=O)\C=C\C1=CC=CC=C1)NC(=S)NC2=CC=CC3=C2N=CC=C3

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID