Salubrinal

Catalog No.S2923

Salubrinal Chemical Structure

Molecular Weight(MW): 479.81

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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In DMSO USD 280 In stock
USD 147 In stock
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Cited by 9 Publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

    PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell NITWdGVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUfLfII2UW6qaXLpeIlwdiCxZjDoeY1idiCOUD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{4{PzFzMjFOwG0> MXvTRW5ITVJ?
human 697 cell NUOzXFg2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlHvTY5pcWKrdHnvckBw\iCqdX3hckA3QTdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lkm4O|g5KM7:TR?= M2LMenNCVkeHUh?=
human ALL-PO cell NGTUWYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPkfnlKUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01NjB6NkW1JO69VQ>? NIf5cG5USU6JRWK=
human ML-2 cell M2r2bWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEXpUoVKdmirYnn0bY9vKG:oIHj1cYFvKE2OLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlYyQDN|IN88US=> MlnPV2FPT0WU
human LB2241-RCC cell M1jFdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3;GWWlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{MkSxMXJESyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwOUi1O|Ih|ryP MYnTRW5ITVJ?
human Becker cell NYPuWYNpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIXnco1KdmirYnn0bY9vKG:oIHj1cYFvKEKnY3vldkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzl2MEGg{txO NYf1eFJqW0GQR1XS
human NEC8 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWe3dIxlUW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4xPTN|MTFOwG0> NVPY[ZU5W0GQR1XS
human Daudi cell M2LEVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGrDUHBKdmirYnn0bY9vKG:oIHj1cYFvKESjdXTpJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4{OzZ7OTFOwG0> NILG[pJUSU6JRWK=
human MHH-PREB-1 cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFvje|hKdmirYnn0bY9vKG:oIHj1cYFvKE2KSD3QVmVDNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lke4PFE4KM7:TR?= Mnn0V2FPT0WU
human NCI-H510A cell MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXXWOHN2UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFUyOEFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15LkCzOVM2KM7:TR?= MWLTRW5ITVJ?
human NCI-H720 cell NVi1[GtsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1nlNmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjV5Mk[4JO69VQ>? NFjYbVZUSU6JRWK=
human MRK-nu-1 cell MmO2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGLGR|FKdmirYnn0bY9vKG:oIHj1cYFvKE2USz3ueU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py55NUG3NUDPxE1? NIn2O3hUSU6JRWK=
human LB2518-MEL cell NVL2PXQ5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGxDOjVzOD3NSWwh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04Njl5M{K2JO69VQ>? MYLTRW5ITVJ?
human BE-13 cell M1jWd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXPCbIJUUW6qaXLpeIlwdiCxZjDoeY1idiCERT2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjZ6NDFOwG0> MXHTRW5ITVJ?
human A3-KAW cell M3mwWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGPQbFdKdmirYnn0bY9vKG:oIHj1cYFvKEF|LVvBW{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPDN{OEmg{txO NIrGVFNUSU6JRWK=
human ST486 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEHtWlJKdmirYnn0bY9vKG:oIHj1cYFvKFOWNEi2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE42OzZ6IN88US=> NVPVcXlmW0GQR1XS
human ES8 cell M2Phcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1TZU2lvcGmkaYTpc44hd2ZiaIXtZY4hTVN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD62OFM{QSEQvF2= MWLTRW5ITVJ?
human HT cell M{PnXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWW3eGYxUW6qaXLpeIlwdiCxZjDoeY1idiCKVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuPVQyQDVizszN MoLDV2FPT0WU
human HAL-01 cell NE\yS4JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHrGUW5KdmirYnn0bY9vKG:oIHj1cYFvKEiDTD2wNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOzF3MEWg{txO MlTkV2FPT0WU
human EB-3 cell M{HOVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnrmTY5pcWKrdHnvckBw\iCqdX3hckBGSi1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT62NlU4OiEQvF2= MnXsV2FPT0WU
human EW-1 cell MlLKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVHsN3lHUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU44OTJ2IN88US=> M4nwd3NCVkeHUh?=
human BL-41 cell NEHGcoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVPJcohq[mm2aX;uJI9nKGi3bXHuJGJNNTRzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT63PFA1QSEQvF2= MX\TRW5ITVJ?
Ramos-2G6-4C10 cell M2Hx[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJHJidW:|LULHOk01SzFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT65NFMyPSEQvF2= M{ntcnNCVkeHUh?=
human DOHH-2 cell M1HJTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVrJcohq[mm2aX;uJI9nKGi3bXHuJGRQUEhvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlIzPjJizszN MWXTRW5ITVJ?
MONO-MAC-6 cell M1mzXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFzlUnJKdmirYnn0bY9vKG:oIHj1cYFvKE2RTl:tUWFENTZiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMD61OFg{KM7:TR?= MYnTRW5ITVJ?
human NKM-1 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHTwWlhKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvPjF7NjFOwG0> MUDTRW5ITVJ?
human HEL cell Mmf6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIn1RpBKdmirYnn0bY9vKG:oIHj1cYFvKEiHTDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlYzPTJizszN MmntV2FPT0WU
human 8-MG-BA cell NWHWXZVHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn3lTY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvQTl7OTFOwG0> NFvIXW1USU6JRWK=
human K-562 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3rhOmlvcGmkaYTpc44hd2ZiaIXtZY4hUy13NkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4xOTlzIN88US=> MmftV2FPT0WU
human LXF-289 cell NXO2NZZlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYHpNnlGUW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwMUW0O{DPxE1? NXnhVlVIW0GQR1XS
human HC-1 cell MoDkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mni3TY5pcWKrdHnvckBw\iCqdX3hckBJSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNVU1PyEQvF2= MVXTRW5ITVJ?
human MC116 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWjJcohq[mm2aX;uJI9nKGi3bXHuJG1EOTF4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlA4OyEQvF2= NHvIOXpUSU6JRWK=
human TE-12 cell NGjzVpdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVfJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuN|M2PyEQvF2= MV7TRW5ITVJ?
human BV-173 cell MkfuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHjkPZBKdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjVyMkGg{txO NFH0fY1USU6JRWK=
human NCCIT cell NUX5S5pyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVXJcohq[mm2aX;uJI9nKGi3bXHuJG5ES0mWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuOlA{QSEQvF2= MmixV2FPT0WU
human CESS cell M{\GSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUnzXnpEUW6qaXLpeIlwdiCxZjDoeY1idiCFRWPTJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjZ4MjFOwG0> MoXEV2FPT0WU
human HUTU-80 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlXKTY5pcWKrdHnvckBw\iCqdX3hckBJXVSXLUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjdyNDFOwG0> MlrrV2FPT0WU
human IST-SL2 cell MnLOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mm\QTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPzdyNDFOwG0> M1rEdXNCVkeHUh?=
human ATN-1 cell M2rr[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWXi[GN2UW6qaXLpeIlwdiCxZjDoeY1idiCDVF6tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjB{Mk[g{txO NIf3dmpUSU6JRWK=
human HCC1599 cell NIfJSVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1PLNmlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFMUW5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjFyOUSg{txO NIW4NWdUSU6JRWK=
human NTERA-S-cl-D1 cell M4GwV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4XmW2lvcGmkaYTpc44hd2ZiaIXtZY4hVlSHUlGtV{1kdC2GMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlg5QDhizszN NEDoR4JUSU6JRWK=
human EVSA-T cell NGXKfGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVfJcohq[mm2aX;uJI9nKGi3bXHuJGVXW0FvVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlkzODRizszN MYnTRW5ITVJ?
human NALM-6 cell NX\Td4NVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJG5CVE1vNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MkBKSzVyPUGzMlA6OjlizszN NH;yeZlUSU6JRWK=
human KMOE-2 cell NYi2fmN6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MljtTY5pcWKrdHnvckBw\iCqdX3hckBMVU:HLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4yODl{IN88US=> NY[wU5l6W0GQR1XS
human HL-60 cell NVXXVpZvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnvZTY5pcWKrdHnvckBw\iCqdX3hckBJVC14MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlM{ODhizszN NVftdYhwW0GQR1XS
human D-542MG cell M4\DUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGQuPTR{TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{41OzB4IN88US=> Mk\VV2FPT0WU
human L-363 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVHJcohq[mm2aX;uJI9nKGi3bXHuJGwuOzZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOVU5OyEQvF2= MYTTRW5ITVJ?
human CMK cell NH;WOYNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFj6WHNKdmirYnn0bY9vKG:oIHj1cYFvKEOPSzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlc1OzZizszN NILTNGJUSU6JRWK=
human IMR-5 cell MofVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2P2T2lvcGmkaYTpc44hd2ZiaIXtZY4hUU2ULUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{44QDR7IN88US=> NGTBU5BUSU6JRWK=
human A101D cell M2nRc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEjpWlRKdmirYnn0bY9vKG:oIHj1cYFvKEFzMEHEJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvQDJyNzFOwG0> MnvOV2FPT0WU
human MOLT-16 cell MoryS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUDJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PjZ3IN88US=> NILkOnFUSU6JRWK=
human LU-139 cell NEnwVJpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGxWNTF|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0MlA1PDRizszN NWexPJZsW0GQR1XS
human OVCAR-4 cell Mo\BS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkTOTY5pcWKrdHnvckBw\iCqdX3hckBQXkODUj20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvPzZyNDFOwG0> MVjTRW5ITVJ?
human BC-3 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml3lTY5pcWKrdHnvckBw\iCqdX3hckBDSy1|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUSuO|k5OiEQvF2= MkDKV2FPT0WU
PC12 cells M3fLUGZ2dmO2aX;uJIF{e2G7 NETUSnVEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDvckBz[XRidIXubYNidXmlaX6geJJm[XSnZDDyZZQheGinb3Podo9ud2O7dH;tZUBk\WyuIHzpcoUhWENzMjDmdo9uKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IFXu[I9xdGG|bXnjJJJmfGmldXz1cUB{fHKnc4OsJGVEPTB;MUWg{txO NEHmOIIyPjByMkK4PC=>
human D-336MG cell NF\xcYtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGi5cHNKdmirYnn0bY9vKG:oIHj1cYFvKERvM{O2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS5zMUO0JO69VQ>? MmrZV2FPT0WU
human BC-1 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYrsR|FkUW6qaXLpeIlwdiCxZjDoeY1idiCEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOTl6IN88US=> NGHQSpdUSU6JRWK=
human KE-37 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVXieY05UW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE2NjJ6MkSg{txO MYfTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
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  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Formulation: in DMEM
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
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    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID