Salubrinal

Catalog No.S2923

Salubrinal Chemical Structure

Molecular Weight(MW): 479.81

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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In DMSO USD 280 In stock
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Cited by 8 Publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

    PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell NHTm[JVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NE\WbJhKdmirYnn0bY9vKG:oIHj1cYFvKEySLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlM4OTF{IN88US=> NVn0XXJpW0GQR1XS
human 697 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYLJcohq[mm2aX;uJI9nKGi3bXHuJFY6PyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwOUi3PFgh|ryP Ml\TV2FPT0WU
human ALL-PO cell NFvwfItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mo\XTY5pcWKrdHnvckBw\iCqdX3hckBCVExvUF:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlA5PjV3IN88US=> MmLVV2FPT0WU
human ML-2 cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkLZTY5pcWKrdHnvckBw\iCqdX3hckBOVC1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND62NVg{OyEQvF2= MnPZV2FPT0WU
human LB2241-RCC cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01Njl6NUeyJO69VQ>? MkXuV2FPT0WU
human Becker cell NIHse5JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFTiTmJKdmirYnn0bY9vKG:oIHj1cYFvKEKnY3vldkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzl2MEGg{txO MljoV2FPT0WU
human NEC8 cell MlXwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYnJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkC1N|MyKM7:TR?= NXyzR49GW0GQR1XS
human Daudi cell NVrTO|BPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVrJcohq[mm2aX;uJI9nKGi3bXHuJGRifWSrIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6zN|Y6QSEQvF2= M2K4dXNCVkeHUh?=
human MHH-PREB-1 cell M{X5V2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX;NPVl1UW6qaXLpeIlwdiCxZjDoeY1idiCPSFitVHJGSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj63PFgyPyEQvF2= NHPEW4hUSU6JRWK=
human NCI-H510A cell NYrkfoc2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXTqUFZOUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFUyOEFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15LkCzOVM2KM7:TR?= M3SxXXNCVkeHUh?=
human NCI-H720 cell NILHeIhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXrnNohSUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFczOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwNUeyOlgh|ryP NIrOW3BUSU6JRWK=
human MRK-nu-1 cell NGrK[GtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1jhOGlvcGmkaYTpc44hd2ZiaIXtZY4hVVKNLX71MVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04Njd3MUexJO69VQ>? MV;TRW5ITVJ?
human LB2518-MEL cell M2nn[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFe0clJKdmirYnn0bY9vKG:oIHj1cYFvKEyEMkWxPE1OTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15Lkm3N|I3KM7:TR?= M37TdnNCVkeHUh?=
human BE-13 cell M4jWWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUnJcohq[mm2aX;uJI9nKGi3bXHuJGJGNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6yOlg1KM7:TR?= M3\XVnNCVkeHUh?=
human A3-KAW cell M3L2fWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NULyTZVYUW6qaXLpeIlwdiCxZjDoeY1idiCDMz3LRXch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjR|Mki5JO69VQ>? M3fXeXNCVkeHUh?=
human ST486 cell NISwUGhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkjGTY5pcWKrdHnvckBw\iCqdX3hckBUXDR6NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuOVM3QCEQvF2= NIXFfHhUSU6JRWK=
human ES8 cell NYPrdWZ1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{\HZ2lvcGmkaYTpc44hd2ZiaIXtZY4hTVN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD62OFM{QSEQvF2= MkPaV2FPT0WU
human HT cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYHJcohq[mm2aX;uJI9nKGi3bXHuJGhVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QC57NEG4OUDPxE1? Mof0V2FPT0WU
human HAL-01 cell Mk\xS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2\FUmlvcGmkaYTpc44hd2ZiaIXtZY4hUEGOLUCxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU4{OTVyNTFOwG0> M3LhXnNCVkeHUh?=
human EB-3 cell NIrtV2VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1TOSWlvcGmkaYTpc44hd2ZiaIXtZY4hTUJvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuOlI2PzJizszN NYHP[oVOW0GQR1XS
human EW-1 cell NF\jb2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{T1TGlvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|EzPCEQvF2= MnvYV2FPT0WU
human BL-41 cell M2rCRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3fMS2lvcGmkaYTpc44hd2ZiaIXtZY4hSkxvNEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25Mlc5ODR7IN88US=> M1TRW3NCVkeHUh?=
Ramos-2G6-4C10 cell Ml\KS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYHhdmJWUW6qaXLpeIlwdiCxZjDoeY1idiCUYX3vd{0zTzZvNFOxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvQTB|MUWg{txO M{jNV3NCVkeHUh?=
human DOHH-2 cell NYP5PW5kT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH[ycYpKdmirYnn0bY9vKG:oIHj1cYFvKESRSFitNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjJ{NkKg{txO M33jUnNCVkeHUh?=
MONO-MAC-6 cell NEDKPXhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnP5TY5pcWKrdHnvckBw\iCqdX3hckBOV06RLV3BR{03KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwNUS4N{DPxE1? NH\PO25USU6JRWK=
human NKM-1 cell M2nvO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVnvOVJ[UW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjZzOU[g{txO M4nCWXNCVkeHUh?=
human HEL cell MlezS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXPJcohq[mm2aX;uJI9nKGi3bXHuJGhGVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyLk[yOVIh|ryP M1jPTXNCVkeHUh?=
human 8-MG-BA cell M{ftV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmDPTY5pcWKrdHnvckBw\iCqdX3hckA5NU2JLVLBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvQTl7OTFOwG0> MUDTRW5ITVJ?
human K-562 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml64TY5pcWKrdHnvckBw\iCqdX3hckBMNTV4MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlAyQTFizszN Mn3CV2FPT0WU
human LXF-289 cell MlPuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmLETY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTV2NzFOwG0> M{DONHNCVkeHUh?=
human HC-1 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Moi1TY5pcWKrdHnvckBw\iCqdX3hckBJSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNVU1PyEQvF2= NHL0bVVUSU6JRWK=
human MC116 cell NFPaXG1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXOwRYE3UW6qaXLpeIlwdiCxZjDoeY1idiCPQ{GxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjJyN{Og{txO M1HRfnNCVkeHUh?=
human TE-12 cell NXG4U45iT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXPJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuN|M2PyEQvF2= NFjwVXZUSU6JRWK=
human BV-173 cell Mn3rS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3m3[GlvcGmkaYTpc44hd2ZiaIXtZY4hSlZvMUezJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPTB{MTFOwG0> NYnBfYxuW0GQR1XS
human NCCIT cell M3HlNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUTJcohq[mm2aX;uJI9nKGi3bXHuJG5ES0mWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuOlA{QSEQvF2= NHT2Z2lUSU6JRWK=
human CESS cell NUm5PVI{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1yxZWlvcGmkaYTpc44hd2ZiaIXtZY4hS0WVUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlY3PjJizszN M{\ieHNCVkeHUh?=
human HUTU-80 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUHJcohq[mm2aX;uJI9nKGi3bXHuJGhWXFVvOECgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU43PzB2IN88US=> NYSxWHh4W0GQR1XS
human IST-SL2 cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MorGTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPzdyNDFOwG0> MojKV2FPT0WU
human ATN-1 cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIeySZFKdmirYnn0bY9vKG:oIHj1cYFvKEGWTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvODJ{NjFOwG0> M{Cz[nNCVkeHUh?=
human HCC1599 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1jh[GlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFMUW5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjFyOUSg{txO NWXFfZZlW0GQR1XS
human NTERA-S-cl-D1 cell MmnqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnTQTY5pcWKrdHnvckBw\iCqdX3hckBPXEWUQT3TMYNtNURzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUKuPFg5QCEQvF2= M3TadnNCVkeHUh?=
human EVSA-T cell NHvnU4FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3rnSWlvcGmkaYTpc44hd2ZiaIXtZY4hTV[VQT3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvQTJyNDFOwG0> MV7TRW5ITVJ?
human NALM-6 cell MoLCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M32wU2lvcGmkaYTpc44hd2ZiaIXtZY4hVkGOTT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZkvKEmFNUC9NVMvODl{OTFOwG0> MnT0V2FPT0WU
human KMOE-2 cell MkDLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmLxTY5pcWKrdHnvckBw\iCqdX3hckBMVU:HLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4yODl{IN88US=> NG\scY5USU6JRWK=
human HL-60 cell NW\2d5pmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUH0[IVIUW6qaXLpeIlwdiCxZjDoeY1idiCKTD22NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjN|MEig{txO NF73cmdUSU6JRWK=
human D-542MG cell M1zxOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEf2dZNKdmirYnn0bY9vKG:oIHj1cYFvKERvNUSyUWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy52M{C2JO69VQ>? MWTTRW5ITVJ?
human L-363 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mm\PTY5pcWKrdHnvckBw\iCqdX3hckBNNTN4MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlU2QDNizszN MUHTRW5ITVJ?
human CMK cell MoW5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWW1VI05UW6qaXLpeIlwdiCxZjDoeY1idiCFTVugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{44PDN4IN88US=> MoX1V2FPT0WU
human IMR-5 cell NWO1U49JT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFzUdVdKdmirYnn0bY9vKG:oIHj1cYFvKEmPUj21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPzh2OTFOwG0> NGHKWXhUSU6JRWK=
human A101D cell M{\kTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{nPXmlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{45OjB5IN88US=> NFr0VmZUSU6JRWK=
human MOLT-16 cell NXTDdnZtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWW2XYw3UW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVE3KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwOU[2OUDPxE1? MWnTRW5ITVJ?
human LU-139 cell M4ezdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGxWNTF|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0MlA1PDRizszN MXrTRW5ITVJ?
human OVCAR-4 cell MoPWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmnHTY5pcWKrdHnvckBw\iCqdX3hckBQXkODUj20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvPzZyNDFOwG0> MV3TRW5ITVJ?
human BC-3 cell M3n6TGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYLJcohq[mm2aX;uJI9nKGi3bXHuJGJENTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zND63PVgzKM7:TR?= NVrKb3dPW0GQR1XS
PC12 cells M3\xV2Z2dmO2aX;uJIF{e2G7 NHrmfXdEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDvckBz[XRidIXubYNidXmlaX6geJJm[XSnZDDyZZQheGinb3Podo9ud2O7dH;tZUBk\WyuIHzpcoUhWENzMjDmdo9uKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IFXu[I9xdGG|bXnjJJJmfGmldXz1cUB{fHKnc4OsJGVEPTB;MUWg{txO NHTBb|gyPjByMkK4PC=>
human D-336MG cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHqxVZBKdmirYnn0bY9vKG:oIHj1cYFvKERvM{O2UWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS5zMUO0JO69VQ>? MXnTRW5ITVJ?
human BC-1 cell M1fTdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFHxPHpKdmirYnn0bY9vKG:oIHj1cYFvKEKFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4yQThizszN NYezVFRHW0GQR1XS
human KE-37 cell NIPGXHlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mnn3TY5pcWKrdHnvckBw\iCqdX3hckBMTS1|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG1MlI5OjRizszN NEOxd2hUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Formulation: in DMEM
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID