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Salubrinal

For research use only.

Catalog No.S2923

18 publications

Salubrinal Chemical Structure

CAS No. 405060-95-9

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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Selleck's Salubrinal has been cited by 18 publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

  • PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{f5UWlvcGmkaYTpc44hd2ZiaIXtZY4hVFBvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuN|cyOTJizszN M1XUbHNCVkeHUh?=
human 697 cell NVGyPFZCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkiyTY5pcWKrdHnvckBw\iCqdX3hckA3QTdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lkm4O|g5KM7:TR?= NEfpOIJUSU6JRWK=
human ALL-PO cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIDnd2JKdmirYnn0bY9vKG:oIHj1cYFvKEGOTD3QU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvODh4NUWg{txO NX\j[lc6W0GQR1XS
human ML-2 cell NX7iSnVLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV3yRllMUW6qaXLpeIlwdiCxZjDoeY1idiCPTD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE43OTh|MzFOwG0> NF3ofndUSU6JRWK=
human LB2241-RCC cell NGiyRodIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFrxd5VKdmirYnn0bY9vKG:oIHj1cYFvKEyEMkK0NU1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lkm4OVczKM7:TR?= NH3JUoZUSU6JRWK=
human Becker cell M3jL[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH7MSJhKdmirYnn0bY9vKG:oIHj1cYFvKEKnY3vldkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvPzl2MEGg{txO NXnTZVFLW0GQR1XS
human NEC8 cell NIj0TGpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGO1SGFKdmirYnn0bY9vKG:oIHj1cYFvKE6HQ{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22MlA2OzNzIN88US=> MlnGV2FPT0WU
human Daudi cell NFPvZ5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGRifWSrIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6zN|Y6QSEQvF2= M4nQXHNCVkeHUh?=
human MHH-PREB-1 cell NX\6NJJMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkLYTY5pcWKrdHnvckBw\iCqdX3hckBOUEhvUGLFRk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi55OEixO{DPxE1? NYDXO|VlW0GQR1XS
human NCI-H510A cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1;GPWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi1NVBCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yM{WzOUDPxE1? NF3OVpdUSU6JRWK=
human NCI-H720 cell NXnHfnpOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{jEZ2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjV5Mk[4JO69VQ>? MkjjV2FPT0WU
human MRK-nu-1 cell MkHGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mor0TY5pcWKrdHnvckBw\iCqdX3hckBOWktvboWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPzVzN{Gg{txO MljzV2FPT0WU
human LB2518-MEL cell NHXRfo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2LuWGlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{NUG4MW1GVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwOUezNlYh|ryP NYDXN3hCW0GQR1XS
human BE-13 cell MkDRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYntPJV{UW6qaXLpeIlwdiCxZjDoeY1idiCERT2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjZ6NDFOwG0> M1HERXNCVkeHUh?=
human A3-KAW cell M4P6Nmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3HlTGlvcGmkaYTpc44hd2ZiaIXtZY4hSTNvS1HXJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE41OzJ6OTFOwG0> Ml\oV2FPT0WU
human ST486 cell NWf1[Xg6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{HNcWlvcGmkaYTpc44hd2ZiaIXtZY4hW1R2OE[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlU{PjhizszN NITZXGpUSU6JRWK=
human ES8 cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3XRc2lvcGmkaYTpc44hd2ZiaIXtZY4hTVN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD62OFM{QSEQvF2= NHPDcoZUSU6JRWK=
human HT cell NEXDRnBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4nST2lvcGmkaYTpc44hd2ZiaIXtZY4hUFRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lkm0NVg2KM7:TR?= NGe2O4dUSU6JRWK=
human HAL-01 cell NV\q[pV[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWDJcohq[mm2aX;uJI9nKGi3bXHuJGhCVC1yMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuN|E2ODVizszN NH\tVY9USU6JRWK=
human EB-3 cell Mn\JS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2XpbWlvcGmkaYTpc44hd2ZiaIXtZY4hTUJvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuOlI2PzJizszN NWnGfI1MW0GQR1XS
human EW-1 cell NYnzRm95T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYLsNnZVUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU44OTJ2IN88US=> MVnTRW5ITVJ?
human BL-41 cell NWfScXNKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUPQS|A2UW6qaXLpeIlwdiCxZjDoeY1idiCETD20NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPzhyNEmg{txO MUXTRW5ITVJ?
Ramos-2G6-4C10 cell M1\OdWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1Pp[mlvcGmkaYTpc44hd2ZiaIXtZY4hWmGvb4OtNmc3NTSFMUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlkxOzF3IN88US=> NH\kSG5USU6JRWK=
human DOHH-2 cell M4DVS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkHaTY5pcWKrdHnvckBw\iCqdX3hckBFV0iKLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4zOjZ{IN88US=> M1P6eXNCVkeHUh?=
MONO-MAC-6 cell NFXwRpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEjadFdKdmirYnn0bY9vKG:oIHj1cYFvKE2RTl:tUWFENTZiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMD61OFg{KM7:TR?= M{LKNXNCVkeHUh?=
human NKM-1 cell M2DWS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUPsSW13UW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjZzOU[g{txO MVHTRW5ITVJ?
human HEL cell NGf1TG9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXftfo1mUW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE43OjV{IN88US=> NYi4N2htW0GQR1XS
human 8-MG-BA cell Mle5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGrLPHdKdmirYnn0bY9vKG:oIHj1cYFvKDhvTVetRmEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC57OUm5JO69VQ>? M{\1WXNCVkeHUh?=
human K-562 cell NXuzTWZ3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlLpTY5pcWKrdHnvckBw\iCqdX3hckBMNTV4MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlAyQTFizszN Ml[0V2FPT0WU
human LXF-289 cell M2\zXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYjpcmJ5UW6qaXLpeIlwdiCxZjDoeY1idiCOWF[tNlg6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwMUW0O{DPxE1? MULTRW5ITVJ?
human HC-1 cell MmHNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3PDbWlvcGmkaYTpc44hd2ZiaIXtZY4hUENvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlE2PDdizszN MV;TRW5ITVJ?
human MC116 cell NVPGWYpwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoHHTY5pcWKrdHnvckBw\iCqdX3hckBOSzFzNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlIxPzNizszN MnnzV2FPT0WU
human TE-12 cell NWDzXI5QT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUXJcohq[mm2aX;uJI9nKGi3bXHuJHRGNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuN|M2PyEQvF2= MXTTRW5ITVJ?
human BV-173 cell M{XwXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUfJcohq[mm2aX;uJI9nKGi3bXHuJGJXNTF5MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlUxOjFizszN M4XZSXNCVkeHUh?=
human NCCIT cell MoC5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmHaTY5pcWKrdHnvckBw\iCqdX3hckBPS0OLVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlYxOzlizszN NWC2bGhwW0GQR1XS
human CESS cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2rWNGlvcGmkaYTpc44hd2ZiaIXtZY4hS0WVUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlY3PjJizszN NGLUdIpUSU6JRWK=
human HUTU-80 cell MoXXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmKxTY5pcWKrdHnvckBw\iCqdX3hckBJXVSXLUiwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjdyNDFOwG0> MV\TRW5ITVJ?
human IST-SL2 cell NWjDfHMyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{Lq[mlvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLWPMNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjd5MESg{txO MUPTRW5ITVJ?
human ATN-1 cell NG\uTIhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlqxTY5pcWKrdHnvckBw\iCqdX3hckBCXE5vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlAzOjZizszN MUHTRW5ITVJ?
human HCC1599 cell NEe0UFdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4rS[WlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFMUW5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjFyOUSg{txO MXfTRW5ITVJ?
human NTERA-S-cl-D1 cell NVLXOI46T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnnkTY5pcWKrdHnvckBw\iCqdX3hckBPXEWUQT3TMYNtNURzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUKuPFg5QCEQvF2= NF2wT4JUSU6JRWK=
human EVSA-T cell NYPkTZVzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXTJcohq[mm2aX;uJI9nKGi3bXHuJGVXW0FvVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlkzODRizszN Mo\sV2FPT0WU
human NALM-6 cell M2XyWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXfJcohq[mm2aX;uJI9nKGi3bXHuJG5CVE1vNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MkBKSzVyPUGzMlA6OjlizszN M4fUUXNCVkeHUh?=
human KMOE-2 cell MkLRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NInKNWRKdmirYnn0bY9vKG:oIHj1cYFvKEuPT1WtNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjFyOUKg{txO NVXURXlMW0GQR1XS
human HL-60 cell M4\BVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWDJcohq[mm2aX;uJI9nKGi3bXHuJGhNNTZyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuN|MxQCEQvF2= NHHnTZJUSU6JRWK=
human D-542MG cell NHi5V3RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUXPfWdMUW6qaXLpeIlwdiCxZjDoeY1idiCGLUW0Nm1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwNEOwOkDPxE1? NYTNboZHW0GQR1XS
human L-363 cell NFPYT21Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEH1TWZKdmirYnn0bY9vKG:oIHj1cYFvKExvM{[zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPTV6MzFOwG0> M1zTdnNCVkeHUh?=
human CMK cell MlfDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4PNXWlvcGmkaYTpc44hd2ZiaIXtZY4hS02NIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuO|Q{PiEQvF2= NWX4eJd1W0GQR1XS
human IMR-5 cell MofNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{XDRWlvcGmkaYTpc44hd2ZiaIXtZY4hUU2ULUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{44QDR7IN88US=> Mmf3V2FPT0WU
human A101D cell MmrTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXPp[ZU3UW6qaXLpeIlwdiCxZjDoeY1idiCDMUCxSEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njh{MEeg{txO NVT2bm1zW0GQR1XS
human MOLT-16 cell M4iyU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYHJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvMU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PjZ3IN88US=> MkPVV2FPT0WU
human LU-139 cell NGfS[IJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH30U41KdmirYnn0bY9vKG:oIHj1cYFvKEyXLUGzPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE1NjB2NESg{txO M{C5bHNCVkeHUh?=
human OVCAR-4 cell NFvKbItIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1n2NWlvcGmkaYTpc44hd2ZiaIXtZY4hV1[FQWKtOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE1Njd4MESg{txO MUfTRW5ITVJ?
human BC-3 cell MkXkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYnDbXozUW6qaXLpeIlwdiCxZjDoeY1idiCEQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvPzl6MjFOwG0> NXfiW3h5W0GQR1XS
PC12 cells NHHsUpZHfW6ldHnvckBie3OjeR?= MWrDfZRweHKxdHXjeIl3\SCjY4Tpeol1gSCxbjDyZZQhfHWwaXPhcZlkcW5idILlZZRm\CC{YYSgdIhmd2Oqcn;tc4N6fG:vYTDj[YxtKGyrbnWgVGMyOiCocn;tJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEWwZH;wcIF{dWmlIILleIlkfWy3bTDzeJJme3NuIFXDOVA:OTVizszN M2P6VFE3ODB{Mki4
human D-336MG cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MljzTY5pcWKrdHnvckBw\iCqdX3hckBFNTN|Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOTF|NDFOwG0> NUfHcVZbW0GQR1XS
human BC-1 cell M3jWTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoHGTY5pcWKrdHnvckBw\iCqdX3hckBDSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNVk5KM7:TR?= NYLPTYtrW0GQR1XS
human KE-37 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NISzNlNKdmirYnn0bY9vKG:oIHj1cYFvKEuHLUO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOjh{NDFOwG0> NFrIe3JUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
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  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Water Insoluble
Ethanol '2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C=C1)C=CC(=O)NC(C(Cl)(Cl)Cl)NC(=S)NC2=CC=CC3=C2N=CC=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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    Volume
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID