Salubrinal

Catalog No.S2923

Salubrinal Chemical Structure

Molecular Weight(MW): 479.81

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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In DMSO USD 280 In stock
USD 147 In stock
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Cited by 8 Publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

    PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

Choose Selective PERK Inhibitors

Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell NWS3RYI1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2PJSGlvcGmkaYTpc44hd2ZiaIXtZY4hVFBvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOuN|cyOTJizszN M4HRUHNCVkeHUh?=
human 697 cell NHPiN5RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml\6TY5pcWKrdHnvckBw\iCqdX3hckA3QTdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Lkm4O|g5KM7:TR?= M{C1fHNCVkeHUh?=
human ALL-PO cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1XqSWlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OE4xQDZ3NTFOwG0> Mlv5V2FPT0WU
human ML-2 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJG1NNTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Lk[xPFM{KM7:TR?= MlLDV2FPT0WU
human LB2241-RCC cell NWO2NFh2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MojRTY5pcWKrdHnvckBw\iCqdX3hckBNSjJ{NEGtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC57OEW3NkDPxE1? MWnTRW5ITVJ?
human Becker cell NVnRVpZmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGJm[2uncjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuO|k1ODFizszN NInRcZlUSU6JRWK=
human NEC8 cell NUTZOlBIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVLJcohq[mm2aX;uJI9nKGi3bXHuJG5GSzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkC1N|MyKM7:TR?= NUXMOGp3W0GQR1XS
human Daudi cell MnWwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVnJcohq[mm2aX;uJI9nKGi3bXHuJGRifWSrIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6zN|Y6QSEQvF2= MW\TRW5ITVJ?
human MHH-PREB-1 cell NVjQ[2hrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVT0RWtqUW6qaXLpeIlwdiCxZjDoeY1idiCPSFitVHJGSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj63PFgyPyEQvF2= NXTRNWl[W0GQR1XS
human NCI-H510A cell NUDlSJh1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnSwTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEWxNGEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjB|NUO1JO69VQ>? M1G2PXNCVkeHUh?=
human NCI-H720 cell MnP6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH3leYtKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IO|IxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py53N{K2PEDPxE1? M3XrSHNCVkeHUh?=
human MRK-nu-1 cell MnHRS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHTQbYVKdmirYnn0bY9vKG:oIHj1cYFvKE2USz3ueU0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py55NUG3NUDPxE1? NXXFV|VUW0GQR1XS
human LB2518-MEL cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{WyfWlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{NUG4MW1GVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwOUezNlYh|ryP NUXOfVc5W0GQR1XS
human BE-13 cell Mn\oS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVjJcohq[mm2aX;uJI9nKGi3bXHuJGJGNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6yOlg1KM7:TR?= NH;aVo1USU6JRWK=
human A3-KAW cell M{i1Rmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3\OZWlvcGmkaYTpc44hd2ZiaIXtZY4hSTNvS1HXJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PE41OzJ6OTFOwG0> NYH5WXJlW0GQR1XS
human ST486 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYDUS2w6UW6qaXLpeIlwdiCxZjDoeY1idiCVVES4OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvPTN4ODFOwG0> MUXTRW5ITVJ?
human ES8 cell NUjjZpdFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX3hOW9GUW6qaXLpeIlwdiCxZjDoeY1idiCHU{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY1OzN7IN88US=> MorZV2FPT0WU
human HT cell NW\QNolST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHrMZ5pKdmirYnn0bY9vKG:oIHj1cYFvKEiWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD65OFE5PSEQvF2= MXrTRW5ITVJ?
human HAL-01 cell M{KzR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIX5WItKdmirYnn0bY9vKG:oIHj1cYFvKEiDTD2wNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOzF3MEWg{txO NUj5OplyW0GQR1XS
human EB-3 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4e0UGlvcGmkaYTpc44hd2ZiaIXtZY4hTUJvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuOlI2PzJizszN MnTCV2FPT0WU
human EW-1 cell NInPe3hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFzvb|FKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlcyOjRizszN MmLJV2FPT0WU
human BL-41 cell M4e5eWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4ewPWlvcGmkaYTpc44hd2ZiaIXtZY4hSkxvNEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25Mlc5ODR7IN88US=> NWDS[XFkW0GQR1XS
Ramos-2G6-4C10 cell Ml7CS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWG4XpBKUW6qaXLpeIlwdiCxZjDoeY1idiCUYX3vd{0zTzZvNFOxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvQTB|MUWg{txO NV3yXJk4W0GQR1XS
human DOHH-2 cell Mk\pS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGRQUEhvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlIzPjJizszN NIX6eIlUSU6JRWK=
MONO-MAC-6 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1XBXGlvcGmkaYTpc44hd2ZiaIXtZY4hVU:QTz3NRWMuPiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFyLkW0PFMh|ryP NEPkTGRUSU6JRWK=
human NKM-1 cell MmrkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mo\HTY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlYyQTZizszN NFLlUZJUSU6JRWK=
human HEL cell NH7EOY1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXrGcHoxUW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE43OjV{IN88US=> NWD0XWNLW0GQR1XS
human 8-MG-BA cell M3zhfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYfJcohq[mm2aX;uJI9nKGi3bXHuJFguVUdvQlGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE46QTl7IN88US=> M4fuU3NCVkeHUh?=
human K-562 cell MmHGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGsuPTZ{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNFE6OSEQvF2= MkHoV2FPT0WU
human LXF-289 cell NXnMS405T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoHuTY5pcWKrdHnvckBw\iCqdX3hckBNYEZvMki5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOTV2NzFOwG0> NH;QZ29USU6JRWK=
human HC-1 cell M3\1Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXjJcohq[mm2aX;uJI9nKGi3bXHuJGhENTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6xOVQ4KM7:TR?= MUTTRW5ITVJ?
human MC116 cell NXjsO5E5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoH6TY5pcWKrdHnvckBw\iCqdX3hckBOSzFzNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlIxPzNizszN NX;LNJU1W0GQR1XS
human TE-12 cell MoLtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4PIfmlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4{OzV5IN88US=> MWPTRW5ITVJ?
human BV-173 cell NECzWY5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmD4TY5pcWKrdHnvckBw\iCqdX3hckBDXi1zN{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU42ODJzIN88US=> M{X3ZnNCVkeHUh?=
human NCCIT cell NGnQbHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJG5ES0mWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuOlA{QSEQvF2= NFfmeYpUSU6JRWK=
human CESS cell MoXIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEHoV29KdmirYnn0bY9vKG:oIHj1cYFvKEOHU2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU43PjZ{IN88US=> Mo[0V2FPT0WU
human HUTU-80 cell NWDhVFY3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{[w[2lvcGmkaYTpc44hd2ZiaIXtZY4hUFWWVT24NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjZ5MESg{txO MVjTRW5ITVJ?
human IST-SL2 cell MoH3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfMTFZKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55N{C0JO69VQ>? MkS2V2FPT0WU
human ATN-1 cell M1;sSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHXLOXdKdmirYnn0bY9vKG:oIHj1cYFvKEGWTj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvODJ{NjFOwG0> NY[3OXBUW0GQR1XS
human HCC1599 cell M4H5NGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHHtWpNKdmirYnn0bY9vKG:oIHj1cYFvKEiFQ{G1PVkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOi5zMEm0JO69VQ>? NVq5R5FQW0GQR1XS
human NTERA-S-cl-D1 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnvjTY5pcWKrdHnvckBw\iCqdX3hckBPXEWUQT3TMYNtNURzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUKuPFg5QCEQvF2= Ml\aV2FPT0WU
human EVSA-T cell M1jHNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2DGZ2lvcGmkaYTpc44hd2ZiaIXtZY4hTV[VQT3UJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVIvQTJyNDFOwG0> NFLyOI1USU6JRWK=
human NALM-6 cell M1jOTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH;0WppKdmirYnn0bY9vKG:oIHj1cYFvKE6DTF2tOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NiCLQ{WwQVE{NjB7Mkmg{txO NYjqVW9LW0GQR1XS
human KMOE-2 cell Ml;XS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnHYTY5pcWKrdHnvckBw\iCqdX3hckBMVU:HLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4yODl{IN88US=> M3vW[XNCVkeHUh?=
human HL-60 cell NX3IUI9WT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIC0fIdKdmirYnn0bY9vKG:oIHj1cYFvKEiOLU[wJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvOzNyODFOwG0> M1jFWXNCVkeHUh?=
human D-542MG cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHm3c4VKdmirYnn0bY9vKG:oIHj1cYFvKERvNUSyUWch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy52M{C2JO69VQ>? NEnGUHZUSU6JRWK=
human L-363 cell NEXTZ2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVHJcohq[mm2aX;uJI9nKGi3bXHuJGwuOzZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOVU5OyEQvF2= MmrJV2FPT0WU
human CMK cell NVvhW|ZDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGNOUyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF|Lke0N|Yh|ryP MWfTRW5ITVJ?
human IMR-5 cell Mn[4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3;NWGlvcGmkaYTpc44hd2ZiaIXtZY4hUU2ULUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{44QDR7IN88US=> NFPj[WlUSU6JRWK=
human A101D cell M4HD[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXTtdXJLUW6qaXLpeIlwdiCxZjDoeY1idiCDMUCxSEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njh{MEeg{txO NHLCWINUSU6JRWK=
human MOLT-16 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MljUTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvQTZ4NTFOwG0> NEHYRlJUSU6JRWK=
human LU-139 cell MkLSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmDvTY5pcWKrdHnvckBw\iCqdX3hckBNXS1zM{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOE4xPDR2IN88US=> NY\NR2dNW0GQR1XS
human OVCAR-4 cell NXzDc3BwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPsNHpZUW6qaXLpeIlwdiCxZjDoeY1idiCRVlPBVk01KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTRwN{[wOEDPxE1? Ml7SV2FPT0WU
human BC-3 cell NX;yfGlWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3;tS2lvcGmkaYTpc44hd2ZiaIXtZY4hSkNvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0Mlc6QDJizszN M{jFOXNCVkeHUh?=
PC12 cells M{LncWZ2dmO2aX;uJIF{e2G7 MkG0R5l1d3C{b4TlZ5RqfmViYXP0bZZqfHlib36gdoF1KHS3bnnjZY16[2mwIITy[YF1\WRicnH0JJBp\W:laILvcY9kgXSxbXGgZ4VtdCCuaX7lJHBEOTJiZoLvcUBieG:ydH;zbZMhcW6mdXPl[EBjgSCHbnTvdIxie22rYzDy[ZRq[3WudX2gd5Rz\XO|LDDFR|UxRTF3IN88US=> M2jCbVE3ODB{Mki4
human D-336MG cell NH\6VVlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV74[IFoUW6qaXLpeIlwdiCxZjDoeY1idiCGLUOzOm1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTVwMUGzOEDPxE1? NV\jUGZJW0GQR1XS
human BC-1 cell Mof0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MojiTY5pcWKrdHnvckBw\iCqdX3hckBDSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNVk5KM7:TR?= MmfmV2FPT0WU
human KE-37 cell NVzpVGF1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVfJcohq[mm2aX;uJI9nKGi3bXHuJGtGNTN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuNlgzPCEQvF2= M2GzZ3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
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  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Formulation: in DMEM
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID