Salubrinal

For research use only.

Catalog No.S2923

17 publications

Salubrinal Chemical Structure

CAS No. 405060-95-9

Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.

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Selleck's Salubrinal has been cited by 17 publications

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  • AngII (30 minutes)-induced Akt activation was attenuated by the ER stress inhibitors 4-PBA (5 mM, 2 hours), TUDCA (300 µg/ml, 1 hour), and salubrinal (30 µM, 1 hour) (n=5; *P<0.01 versus con, #P<0.05 versus Ang)

    J Am Soc Nephrol, 2015, 26(8):1839-54. . Salubrinal purchased from Selleck.

  • PC-3 and HCT-116 p53+/+ cells were treated with 3-AWA and/or salubrinal for the indicated time point. The protein-specific antibodies were used to analyze the expression of the given proteins through immunoblotting.

    Oncogenesis, 2017, 6(5):e341. Salubrinal purchased from Selleck.

  • HEK293 cells were transfected with or without Flag-VP1 for 24 h, then treated with d Salubrinal (Sal, 75 μM) for 8 h followed by immunoblot detection. All data in this figure represent the mean ± SEM of three independent experiments. *p<0.05; ***p<0.01.

    Neurotox Res, 2017, 31(2):204-217. Salubrinal purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Salubrinal is a selective inhibitor of eIF2α dephosphorylation and inhibits ER stress-mediated apoptosis with EC50 of ~15 μM in a cell-free assay.
Targets
eIF2α [1]
(Cell-free assay)
In vitro

Salubrinal is a selective inhibitor of cellular complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Salubrinal inhibited ER stress-mediated apoptosis induced by the protein glycosylation inhibitor tunicamycin (Tm) in a dose-dependent manner, with a median effective concentration (EC50) ∼ 15 μM. Salubrinal also suppressed Tm-induced DNA fragmentation the processing of caspase-7, a caspase activated by ER stress. However, Salubrinal is not a general apoptosis inhibitor. Salubrinal induced rapid and robust eIF2α phosphorylation and its downstream effects in PC12 cells, including down-regulation of cyclin D1 and up-regulation of GADD34 and CHOP, two proteins whose expression is induced by eIF2α phosphorylation. Salubrinal inhibits eIF2α dephosphorylation by inhibiting the PP1/GADD34 complex. Salubrinal inhibits HSV replication with IC50 of ~ 3μM by inhibiting eIF2α dephosphorylation. [1] Salubrinal increased non-rapid eye movement (NREM) sleep. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human LP-1 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVrJcohq[mm2aX;uJI9nKGi3bXHuJGxRNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|LkO3NVEzKM7:TR?= MWrTRW5ITVJ?
human 697 cell MkXLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUjDe3pHUW6qaXLpeIlwdiCxZjDoeY1idiB4OUegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zMlk5Pzh6IN88US=> NWTqOGhJW0GQR1XS
human ALL-PO cell NGLFc2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEnBbnFKdmirYnn0bY9vKG:oIHj1cYFvKEGOTD3QU{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQvODh4NUWg{txO NXHrRohtW0GQR1XS
human ML-2 cell NFTRe4ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NELJWpNKdmirYnn0bY9vKG:oIHj1cYFvKE2OLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20MlYyQDN|IN88US=> NWL0VnljW0GQR1XS
human LB2241-RCC cell NHuwXnpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkjWTY5pcWKrdHnvckBw\iCqdX3hckBNSjJ{NEGtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC57OEW3NkDPxE1? M3nrXHNCVkeHUh?=
human Becker cell NYjGZplPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV[0U2VTUW6qaXLpeIlwdiCxZjDoeY1idiCEZXPr[ZIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02Njd7NECxJO69VQ>? MojPV2FPT0WU
human NEC8 cell M4DxN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV3VNpNQUW6qaXLpeIlwdiCxZjDoeY1idiCQRVO4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok4xPTN|MTFOwG0> M1fGN3NCVkeHUh?=
human Daudi cell Ml3hS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYXJcohq[mm2aX;uJI9nKGi3bXHuJGRifWSrIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nj6zN|Y6QSEQvF2= MWDTRW5ITVJ?
human MHH-PREB-1 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGr2bWJKdmirYnn0bY9vKG:oIHj1cYFvKE2KSD3QVmVDNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Lke4PFE4KM7:TR?= M2jPbHNCVkeHUh?=
human NCI-H510A cell M1fvO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3PrSWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi1NVBCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Py5yM{WzOUDPxE1? M4HkVXNCVkeHUh?=
human NCI-H720 cell M{\Eemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M17wcmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF04NjV5Mk[4JO69VQ>? NFH5W3VUSU6JRWK=
human MRK-nu-1 cell NVzmdJBCT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mkj5TY5pcWKrdHnvckBw\iCqdX3hckBOWktvboWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPzVzN{Gg{txO NFjaR2VUSU6JRWK=
human LB2518-MEL cell NGPPcZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3zCPGlvcGmkaYTpc44hd2ZiaIXtZY4hVEJ{NUG4MW1GVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwOUezNlYh|ryP NHLzPG9USU6JRWK=
human BE-13 cell M1raUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkLsTY5pcWKrdHnvckBw\iCqdX3hckBDTS1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlY5PCEQvF2= MnS4V2FPT0WU
human A3-KAW cell NH;sZllIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXTXOlFMUW6qaXLpeIlwdiCxZjDoeY1idiCDMz3LRXch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjR|Mki5JO69VQ>? NYDFU2l4W0GQR1XS
human ST486 cell NGXPcnlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJHNVPDh4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD61N|Y5KM7:TR?= MkTPV2FPT0WU
human ES8 cell NWfa[o86T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXKwSIlKUW6qaXLpeIlwdiCxZjDoeY1idiCHU{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24MlY1OzN7IN88US=> NHO3eGZUSU6JRWK=
human HT cell NFfUPIZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{LkOGlvcGmkaYTpc44hd2ZiaIXtZY4hUFRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16Lkm0NVg2KM7:TR?= M1GwVnNCVkeHUh?=
human HAL-01 cell NHLGZZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXTNOpYxUW6qaXLpeIlwdiCxZjDoeY1idiCKQVytNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjNzNUC1JO69VQ>? NH\MbG9USU6JRWK=
human EB-3 cell M4HaWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV\WelR2UW6qaXLpeIlwdiCxZjDoeY1idiCHQj2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU43OjV5MjFOwG0> M1zJc3NCVkeHUh?=
human EW-1 cell NVyyeYhmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NULSd2JxUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU44OTJ2IN88US=> NEfwXYRUSU6JRWK=
human BL-41 cell NEW3fGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlnsTY5pcWKrdHnvckBw\iCqdX3hckBDVC12MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuO|gxPDlizszN NVPYTlZIW0GQR1XS
Ramos-2G6-4C10 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmXkTY5pcWKrdHnvckBw\iCqdX3hckBT[W2xcz2yS|YuPENzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUmuPVA{OTVizszN NWTzR44{W0GQR1XS
human DOHH-2 cell NX:2dVJHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX\RSo91UW6qaXLpeIlwdiCxZjDoeY1idiCGT1jIMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5{Mk[yJO69VQ>? MUDTRW5ITVJ?
MONO-MAC-6 cell Ml:1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWrD[lg5UW6qaXLpeIlwdiCxZjDoeY1idiCPT17PMW1CSy14IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuOVQ5OyEQvF2= MWXTRW5ITVJ?
human NKM-1 cell M3jOb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoHnTY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlYyQTZizszN M3nTbXNCVkeHUh?=
human HEL cell NIDCZZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVzKOoRNUW6qaXLpeIlwdiCxZjDoeY1idiCKRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE43OjV{IN88US=> MXjTRW5ITVJ?
human 8-MG-BA cell MkDkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYLSRllsUW6qaXLpeIlwdiCxZjDoeY1idiB6LV3HMWJCKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBwOUm5PUDPxE1? MojrV2FPT0WU
human K-562 cell NIXyVGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4DhSGlvcGmkaYTpc44hd2ZiaIXtZY4hUy13NkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4xOTlzIN88US=> MnvQV2FPT0WU
human LXF-289 cell NEO1ZoFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXLJcohq[mm2aX;uJI9nKGi3bXHuJGxZTi1{OEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4yPTR5IN88US=> MV7TRW5ITVJ?
human HC-1 cell NEPMbIpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVvJcohq[mm2aX;uJI9nKGi3bXHuJGhENTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6xOVQ4KM7:TR?= MVvTRW5ITVJ?
human MC116 cell NGrteXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUXUPJMzUW6qaXLpeIlwdiCxZjDoeY1idiCPQ{GxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjJyN{Og{txO NFHZbodUSU6JRWK=
human TE-12 cell Ml20S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUX0Too4UW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjN|NUeg{txO MoXCV2FPT0WU
human BV-173 cell NX24d45iT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlP3TY5pcWKrdHnvckBw\iCqdX3hckBDXi1zN{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU42ODJzIN88US=> NVrScHhPW0GQR1XS
human NCCIT cell NIXYXGNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn3ITY5pcWKrdHnvckBw\iCqdX3hckBPS0OLVDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlYxOzlizszN MmDzV2FPT0WU
human CESS cell NWPoW|V[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUnJcohq[mm2aX;uJI9nKGi3bXHuJGNGW1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT62OlYzKM7:TR?= M{G2Z3NCVkeHUh?=
human HUTU-80 cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXHnU|VrUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTFwNkewOEDPxE1? MU\TRW5ITVJ?
human IST-SL2 cell NIK5[ZlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Moi5TY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPzdyNDFOwG0> NWTJN5pWW0GQR1XS
human ATN-1 cell NVHYdZl3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3XlOWlvcGmkaYTpc44hd2ZiaIXtZY4hSVSQLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNk4xOjJ4IN88US=> MmLkV2FPT0WU
human HCC1599 cell M4rjbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{T1VWlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFMUW5PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjFyOUSg{txO M1zXbnNCVkeHUh?=
human NTERA-S-cl-D1 cell NXTROopYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2XnUmlvcGmkaYTpc44hd2ZiaIXtZY4hVlSHUlGtV{1kdC2GMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGyMlg5QDhizszN NU\aXlhwW0GQR1XS
human EVSA-T cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVzOT4YzUW6qaXLpeIlwdiCxZjDoeY1idiCHVmPBMXQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOi57MkC0JO69VQ>? NHrGZmtUSU6JRWK=
human NALM-6 cell NUHxNpI2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUjhR217UW6qaXLpeIlwdiCxZjDoeY1idiCQQVzNMVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigS5iSVO1NF0yOy5yOUK5JO69VQ>? NH3WN|hUSU6JRWK=
human KMOE-2 cell NUXXOmE3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWXlTVJOUW6qaXLpeIlwdiCxZjDoeY1idiCNTV;FMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy5zMEmyJO69VQ>? MYXTRW5ITVJ?
human HL-60 cell NXn1UJZKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn\5TY5pcWKrdHnvckBw\iCqdX3hckBJVC14MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlM{ODhizszN NVfjUZppW0GQR1XS
human D-542MG cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUXJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTR{TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{41OzB4IN88US=> MV7TRW5ITVJ?
human L-363 cell NYXaPIhJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXrJcohq[mm2aX;uJI9nKGi3bXHuJGwuOzZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOVU5OyEQvF2= NFjNSJJUSU6JRWK=
human CMK cell NWHhSHZwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFK2OlZKdmirYnn0bY9vKG:oIHj1cYFvKEOPSzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlc1OzZizszN M1nTUnNCVkeHUh?=
human IMR-5 cell MlO5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NInhWJJKdmirYnn0bY9vKG:oIHj1cYFvKEmPUj21JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvPzh2OTFOwG0> NUTGcGNHW0GQR1XS
human A101D cell MmGxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4\adGlvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{45OjB5IN88US=> Ml7sV2FPT0WU
human MOLT-16 cell NIj2[o1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGnyOWtKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy57Nk[1JO69VQ>? NV\yUIpKW0GQR1XS
human LU-139 cell NX;YTYw3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4OxfWlvcGmkaYTpc44hd2ZiaIXtZY4hVFVvMUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvODR2NDFOwG0> M{LGRXNCVkeHUh?=
human OVCAR-4 cell NHzxe4VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXzKSmh7UW6qaXLpeIlwdiCxZjDoeY1idiCRVlPBVk01KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTRwN{[wOEDPxE1? NHXXWmxUSU6JRWK=
human BC-3 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{\RU2lvcGmkaYTpc44hd2ZiaIXtZY4hSkNvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0Mlc6QDJizszN NG[wfXJUSU6JRWK=
PC12 cells MlXpSpVv[3Srb36gZZN{[Xl? NYP4S3F{S3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgc44hemG2IIT1colk[W27Y3nuJJRz\WG2ZXSgdoF1KHCqZX;jbJJwdW:leYTvcYEh[2WubDDsbY5mKFCFMUKg[pJwdSCjcH;weI9{cXNiaX7keYNm\CCkeTDFcoRweGyjc33pZ{Bz\XSrY4XseY0he3S{ZYPzMEBGSzVyPUG1JO69VQ>? Mlm2NVYxODJ{OEi=
human D-336MG cell NX3KVHRbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKGi3bXHuJGQuOzN4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4yOTN2IN88US=> MnzKV2FPT0WU
human BC-1 cell NWrpdZhpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NITGNIRKdmirYnn0bY9vKG:oIHj1cYFvKEKFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU4yQThizszN MkXpV2FPT0WU
human KE-37 cell MkHuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV7Rc|JFUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE2NjJ6MkSg{txO NIDtcoZUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 ; 

PubMed: 27729194     


OVCAR10 cells were incubated with sub-lethal dose of Salubrinal [20 μM] for 1, 3, 6, 12, 24 and 48 h. Whole cell lysates were subjected to Western blot analysis and probed with the above antibodies.

HSF1 / p-HSF1 / ATF3 / c-fos / NFATc1 / RelA ; 

PubMed: 27189267     


The accumulation of cellular transcription factors in nucleus was analyzed after treatment with salubrinal for 4 hours.

GRP78 / p-eIF2α / CHOP ; 

PubMed: 24525736     


HepG2 cells were treated with salubrinal at the indicated dosages for 24 h. Total proteins were harvested and subjected to western blotting analysis of GRP78, P-eIF2α, CHOP and β-actin. 

27729194 27189267 24525736
In vivo Salubrinal inhibits HSV replication in a mouse cornea infection model. Compared to vehicle control, topical Salubrinal treatment significantly reduced the viral titer recovered from eye swabs of infected animals. [1] I.C.V. administration of Salubrinal significantly modified the homeostatic sleep response. [3]

Protocol

Cell Research:[1]
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  • Cell lines: PC12
  • Concentrations: 0-100 μM
  • Incubation Time: 48 hours
  • Method: PC12 cells are plated in 384-well plates at 5000 cells per well in 40μL phenol red-freemedium containing 3μg/ml Tm to induce ER stress. 100 nL of the DiverSet E (5 mg/mlin DMSO) or National Cancer Institute’s (NCI) Structural Diversity set and Open Collections (10 mM in DMSO) (NCI) are added to the wells by robotic pin transfer. After 48 hours, cell viability is assessed using a luminescence-based ATP assay. DMSO- and zVAD.fmk-treated wells on each plate served as negative and positive controls for rescue from ER stress-induced ATP loss, respectively.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Eight-week-old male CD-1 outbred mice
  • Dosages: 75μM
  • Administration: On cornea
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (197.99 mM)
Ethanol 2 mg/mL (4.16 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 479.81
Formula

C21H17Cl3N4OS

CAS No. 405060-95-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C=C1)C=CC(=O)NC(C(Cl)(Cl)Cl)NC(=S)NC2=CC=CC3=C2N=CC=C3

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID