research use only

GPNA hydrochloride Amino acid transporter inhibitor

Name: GPNA hydrochloride
Brand: Selleck Chemicals
SKU: S6670
Availability: InStock
Rating: 5 (7 reviews)

Cat.No.S6670

GPNA hydrochloride (L-γ-glutamyl-p-nitroanilide hydrochloride) is a substrate of γ-glutamyltransferase (GGT) and an inhibitor of the glutamine transporter ASCT2 with an IC50 of ~250 μM in A549 cells. It also inhibits Na+-dependent carriers SNAT1, -2, -4, -5 and Na+-independent leucine transporters LAT1/2. It can be widely used in research on pancreatic cancer, breast cancer, glioblastoma multiforme, acute myelogenous leukemia, and non-small-cell lung cancer.

Chemical Structure

Molecular Weight: 303.7

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.9%

99.9

Related Targets	CD markers AChR Calcium Channel Sodium Channel Potassium Channel GABA Receptor TRP Channel ATPase GluR NMDAR P2 Receptor Proton Pump P-gp CFTR SGLT Chloride Channel MCT GLUT CRM1 VDAC BCRP NKCC OCT NCX OAT VMAT Aquaporin EAAT GlyT GlyR
Related Products	Nanvuranlat (JPH203) V-9302 BCH xCT/SLC7A11 Antibody [E20L8] ASCT2 Antibody [H8L8] SLC1A5/ASCT2 Antibody [F12D14] 4F2hc/CD98 Antibody [J3D15] SLC7A5/LAT1 Antibody [N14A22] xCT/SLC7A11 Antibody [H13H3] EAAT2 Antibody [C5P1] VGLUT2 Antibody [J20C3]

Chemical Information, Storage & Stability

Molecular Weight	303.7	Formula	C₁₁H₁₄ClN₃O₅	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	67953-08-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

DMSO : 5 mg/mL (16.46 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (0.82mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (0.82mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	ASCT2 ^[1] (Cell-free assay) 55 μM(Ki)
In vitro	GPNA leads to a significant decrease in endometrial cancer cell growth and spheroid cross-sectional area.^[1]
In vivo	EGFRAS-GPNA (EGFRAS-specific GPNA oligomers) is taken-up by several tissues including the xenograft tumor. Systemic administration of EGFRAS-GPNA induces antitumor effects in HNSCC xenografts, with similar efficacies as the FDA-approved EGFR inhibitors: cetuximab and erlotinib. EGFRAS-GPNA effectively againsts the constitutively active EGFR vIII mutant implicated in cetuximab resistance.
References	[1] https://pubmed.ncbi.nlm.nih.gov/30696905/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):