Glucosamine sulfate

Catalog No.S3850 Synonyms: D-Glucosaminesulfate, D-Glucosamine sulphate

For research use only.

Glucosamine sulfate (D-Glucosaminesulfate, D-Glucosamine sulphate) is a dietary supplement commonly used in the treatment of osteoarthritis. It is a type of glycosaminoglycan, which is a naturally occurring major component of joint cartilage and joint fluid.

Glucosamine sulfate Chemical Structure

CAS No. 29031-19-4

Purity & Quality Control

Related Other Products

Biological Activity

Description Glucosamine sulfate (D-Glucosaminesulfate, D-Glucosamine sulphate) is a dietary supplement commonly used in the treatment of osteoarthritis. It is a type of glycosaminoglycan, which is a naturally occurring major component of joint cartilage and joint fluid.
In vitro

Glucosamine sulfate exerts a pronounced suppressive effect on MMPs in osteosarcoma cell lines in vitro[1]. Glucosamine suppresses the activation of T-lymphoblasts and dendritic cells in vitro as well as allogeneic mixed leukocyte reactivity in a dose-dependent manner. Glucosamine has been shown to inhibit nucleic acid and protein biosynthesis and irreversible damage to organelles in tumor cells, but not in normal cells[3].

In vivo Glucosamine sulfate attenuates DSS-induced colitis symptoms, possibly by suppressing the NF-κB-mediated TNF-α and IL-1β production and neutrophil activation that contribute to intestinal barrier disruption in colitic mice[2]. Glucosamine administration prolongs allogeneic cardiac allograft survival in vivo[3].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: MG-63 and SaOS-2 cells
  • Concentrations: 0, 10, 50 and 100 μg/ml
  • Incubation Time: 48 h
  • Method: Cells are exposed to 0, 10, 50 and 100 μg/ml glucosamine sulfate for 48 h and treatment toxicity is determined through measurement of cell viability and proliferation. Relative gene expression of matrix metalloproteinase (MMP)-2, -3 and -9 is quantified by real-time polymerase chain reaction. Protein levels of MMP-2 and -9 are assessed by ELISA.
Animal Research:[2]
  • Animal Models: C57BL/6 mice
  • Dosages: 0, 0.05, and 0.10% (w/w)
  • Administration: oral

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 277.25
Formula

C6H13NO5.H2SO4

CAS No. 29031-19-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(C(C(C(C(C=O)N)O)O)O)O.OS(=O)(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03827161 Completed Dietary Supplement: Glucosamine|Other: Placebo Healthy Subject|Overweight Fred Hutchinson Cancer Center August 1 2017 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Glucosamine sulfate | Glucosamine sulfate supplier | purchase Glucosamine sulfate | Glucosamine sulfate cost | Glucosamine sulfate manufacturer | order Glucosamine sulfate | Glucosamine sulfate distributor