Gamma-Oryzanol

Catalog No.S3957 Batch:S395701

Technical Data

Formula	C₄₀H₅₈O₄
Molecular Weight	602.89	CAS No.	11042-64-1
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (165.86 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Gamma-oryzanol (GO), a nutriactive phytochemical naturally occurring in crude rice bran oil, is an antioxidant compound used to treat hyperlipidemia, disorders of menopause and to increase the muscle mass. γ-Oryzanol is a potent DNA methyltransferases (DNMTs) inhibitor in the striatum of mice. γ-Oryzanol significantly inhibits the activities of DNMT1 (IC50=3.2 μM), DNMT3a (IC50=22.3 μM).
In vitro	From the proliferation assay using MTT reagent, the effect of gamma-oryzanol on DU145 and PC3 are slightly different with an IC50 after 7 days of exposure at 0.52±0.06 and 0.40±0.08 mg/ml, respectively. For in vitro condition in proliferation assay, IC50 values seem to be high in the scale of mg/ml. gamma-oryzanol may affect to prostate cancer cells through the down regulation of some antioxidant genes, CAT and GPX^[1].
In vivo	In tumor-bearing mice, gamma-oryzanol can reduce tumor mass associated with pro-angiogenic biomarkers^[1]. Gamma-oryzanol is not carcinogenic and are safe for animals. In vivo trials proved gamma-oryzanol to be powerful antioxidant and also appears to be a very promising anticancerogenic compound. Supplementation of gamma-oryzanol in diabetic patients may improve metabolic dysfunctions such as hyperglycaemia, hypercholesterolaemia, hypertriglyceridaemia, insulin resistance and may alleviate diabetic complications resulting from lipid peroxidation and free radicals^[3].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines human DU145 and PC3 prostate cancer cells Concentrations 0.1-2.0mg/ml Incubation Time 24 h Method The prostate cancer cells at a density of 3.5×10⁴ cells/well in serum-containing medium are seeded in 24-well microplates and grown to 80-90% confluency. Then, they are treated with gamma-oryzanol at various concentrations in a humidified atmosphere of 5%CO2 at 37°C for 24h. After the incubation, cells are washed twice and 150μl of culture medium containing 1mg/ml of MTT dye is added to each well and further incubated for 4h. The medium containing MTT dye is then replaced with 150μl of DMSO. The blue color of the oxidized MTT (formazan) as determined by measuring the absorbance at 570 nm using ELISA microplate reader.

Cell Assay:^{^[1]}

Cell lines
human DU145 and PC3 prostate cancer cells
Concentrations
0.1-2.0mg/ml
Incubation Time
24 h
Method
The prostate cancer cells at a density of 3.5×10⁴ cells/well in serum-containing medium are seeded in 24-well microplates and grown to 80-90% confluency. Then, they are treated with gamma-oryzanol at various concentrations in a humidified atmosphere of 5%CO2 at 37°C for 24h. After the incubation, cells are washed twice and 150μl of culture medium containing 1mg/ml of MTT dye is added to each well and further incubated for 4h. The medium containing MTT dye is then replaced with 150μl of DMSO. The blue color of the oxidized MTT (formazan) as determined by measuring the absorbance at 570 nm using ELISA microplate reader.

References

Selleck's Gamma-Oryzanol has been cited by 3 publications

Rice Bran Extract Suppresses High-Fat Diet-Induced Hyperlipidemia and Hepatosteatosis through Targeting AMPK and STAT3 Signaling [ Nutrients, 2023, 15(16)3630]	PubMed: 37630819
Rice Bran Extract Suppresses High-Fat Diet-Induced Hyperlipidemia and Hepatosteatosis through Targeting AMPK and STAT3 Signaling [ Nutrients, 2023, 15(16)3630]	PubMed: 37630819
SChLAP1 promotes prostate cancer development through interacting with EZH2 to mediate promoter methylation modification of multiple miRNAs of chromosome 5 with a DNMT3a-feedback loop [ Cell Death Dis, 2021, 12(2):188]	PubMed: 33589600

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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