For research use only.

Catalog No.S5488 Synonyms: Solvent Yellow 94, Resorcinolphthalein, Yellow fluorescein

Fluorescein Chemical Structure

Molecular Weight(MW): 332.31

Fluorescein is a manufactured organic compound and dye.

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USD 97 In stock
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Biological Activity

Description Fluorescein is a manufactured organic compound and dye.



Solubility (25°C)

In vitro DMSO 67 mg/mL (201.61 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 332.31


CAS No. 2321-07-5
Storage powder
in solvent
Synonyms Solvent Yellow 94, Resorcinolphthalein, Yellow fluorescein
Smiles OC1=CC2=C(C=C1)C3(OC(=O)C4=C3C=CC=C4)C5=C(O2)C=C(O)C=C5

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03953703 Not yet recruiting Drug: Levocarnitine|Drug: Placebo Sjogren''s Syndrome|Keratoconjunctivitis Sicca Vanderbilt University Medical Center July 1 2020 Phase 2
NCT04354545 Not yet recruiting Drug: Xiidra (Lifitegrast ophthalmic solution) 5% Glaucoma University of California Los Angeles|Novartis June 1 2020 --
NCT04139798 Not yet recruiting Drug: NOV03|Drug: Placebo Dry Eye Disease (DED) Bausch Health Americas Inc. May 2020 Phase 3
NCT04339764 Recruiting Combination Product: iPSC-derived RPE/PLGA transplantation Age-Related Macular Degeneration National Eye Institute (NEI)|National Institutes of Health Clinical Center (CC) May 19 2020 Phase 1|Phase 2
NCT04357795 Not yet recruiting Drug: CequaTM (Cyclosporine 0.09%) ophthalmic solution Dry Eye Disease Sun Pharma Global FZE May 2020 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID