Home DNA Damage/DNA Repair Pax inhibitor EG1 For research use only.

EG1

EG1 is a specific Paired box 2 (Pax2) inhibitor that targets the DNA binding domain and inhibits embryonic kidney development.

EG1 Chemical Structure

EG1 Chemical Structure

CAS: 693241-54-2

Purity & Quality Control

Batch: Purity: 98.35%
98.35

EG1 Related Products

Choose Selective Pax Inhibitors

Biological Activity

Description EG1 is a specific Paired box 2 (Pax2) inhibitor that targets the DNA binding domain and inhibits embryonic kidney development.
Targets
Pax2 [1]
In vitro
In vitro

EG1, a specific and efficacious Pax2 inhibitor that phenocopies a genetic loss of function, is capable of inhibiting Pax2 mediated expression of EGFP without significant effects on Pax2 protein levels.[1]
Cell Research Cell lines HEK293 cells containing PRS-EGFP reporter
Concentrations 0.1, 0.5, 1, 5, 10, 50, 100 and 500 µM
Incubation Time --
Method

PRS4-EGFP cells transfected with CMV-Pax2b or CMV-Pax2bC63Y (Mut) and treated with increasing concentrations of EG1. The media were removed from test plates, and cells were lysed in 2×SDS buffer. Total protein was separated on 4-12% Bis-Tris gels, transferred to PVDF membranes, and immunoblotted with anti-Pax2 antibody,20 anti-EGFP antibody, antiactin, anti-P-Histone H3, anti-Histone H3, or anti-Actin.

Chemical Information & Solubility

Molecular Weight 390.39 Formula

C22H18N2O5
CAS No. 693241-54-2 SDF --
Smiles COC1=CC=CC=C1C(=O)NC2=CC=C(C=C2)C(=O)NC3=CC=CC=C3C(O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 78 mg/mL ( (199.8 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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