EG1

Catalog No.S0858 Batch:S085802

Technical Data

Formula	C₂₂H₁₈N₂O₅
Molecular Weight	390.39	CAS No.	693241-54-2
Solubility (25°C)*	In vitro	DMSO	78 mg/mL (199.8 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

EG1 is a specific Paired box 2 (Pax2) inhibitor that targets the DNA binding domain and inhibits embryonic kidney development.

Targets

Pax2 ^[1]

In vitro

EG1, a specific and efficacious Pax2 inhibitor that phenocopies a genetic loss of function, is capable of inhibiting Pax2 mediated expression of EGFP without significant effects on Pax2 protein levels.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HEK293 cells containing PRS-EGFP reporter Concentrations 0.1, 0.5, 1, 5, 10, 50, 100 and 500 µM Incubation Time -- Method PRS4-EGFP cells transfected with CMV-Pax2b or CMV-Pax2b^C63Y (Mut) and treated with increasing concentrations of EG1. The media were removed from test plates, and cells were lysed in 2×SDS buffer. Total protein was separated on 4-12% Bis-Tris gels, transferred to PVDF membranes, and immunoblotted with anti-Pax2 antibody,20 anti-EGFP antibody, antiactin, anti-P-Histone H3, anti-Histone H3, or anti-Actin.

Cell Assay:^{^[1]}

Cell lines
HEK293 cells containing PRS-EGFP reporter
Concentrations
0.1, 0.5, 1, 5, 10, 50, 100 and 500 µM
Incubation Time
--
Method
PRS4-EGFP cells transfected with CMV-Pax2b or CMV-Pax2b^C63Y (Mut) and treated with increasing concentrations of EG1. The media were removed from test plates, and cells were lysed in 2×SDS buffer. Total protein was separated on 4-12% Bis-Tris gels, transferred to PVDF membranes, and immunoblotted with anti-Pax2 antibody,20 anti-EGFP antibody, antiactin, anti-P-Histone H3, anti-Histone H3, or anti-Actin.

References

[1] Edward Grimley, et al. ACS Chem Biol. 2017 Mar 17;12(3):724-734.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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