Molecular Weight: 315.41 g/mol

Dihydroethidium Chemical Structure
Chemical Structure
Check the Dihydroethidium product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 63 mg/mL (199.74 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 2 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Chemical Information

IUPAC Name 5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine
CAS Number 104821-25-2
Molecular Formula

C21H21N3
Molecular Weight (MW) 315.41
SMILES CCN1C(C2=CC=CC=C2)C3=C(C=CC(=C3)N)C4=C1C=C(N)C=C4
InChIKey XYJODUBPWNZLML-UHFFFAOYSA-N

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Note: Technical data last updated: Sep 1, 2026.