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Dehydroandrographolide Chloride Channel inhibitor

Cat.No.S3807

Dehydroandrographolide, isolated from Andrographis paniculata (Burm. F.) Nees (Chuan-xin-lian), is a novel TMEM16A inhibitor and possesses multiple pharmacological activities, including anti-inflammation, anti-cancer, anti-bacterial, anti-virus and anti-hepatitis activity.
Dehydroandrographolide Chloride Channel inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 332.43

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Quality Control

Batch: Purity: 99.99%
99.99

Solubility

In vitro
Batch:

DMSO : 66 mg/mL (198.53 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 33 mg/mL

Water : Insoluble

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 332.43 Formula

C20H28O4

 Storage (From the date of receipt)
CAS No. 134418-28-3 Download SDF Storage of Stock Solutions

Synonyms N/A Smiles CC12CCC(C(C1CCC(=C)C2CC=C3C=COC3=O)(C)CO)O

Mechanism of Action

Targets/IC50/Ki
TMEM16A
In vitro
Dehydroandrographolide inhibits TMEM16A chloride currents in Fisher rat thyroid (FRT) cells that are transfected stably with human TMEM16A and in TMEM16A-overexpressed SW620 cells but does not alter cystic fibrosis transmembrane conductance regulator (CFTR) chloride currents. Further functional studies show that this compound suppresses the proliferation of SW620 cells in a dose- and time-dependent manner using MTT assays. It significantly inhibits migration and invasion of SW620 cells as detected by wound-healing and transwell assays. Moreover, treatment of SW620 cells with this chemical leads to a decrease in TMEM16A protein levels but has no effect on TMEM16A mRNA levels. This compound induces autophagy in human oral cancer cells by modulating p53 expression, activating JNK1/2, and inhibiting Akt and p38. It is an iNOS inhibitor and an antiinflammatory and antiviral agent.
In vivo
Administration of dehydroandrographolide effectively suppresses the tumor formation in the oral carcinoma xenograft model in vivo.
References

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