Home Metabolism SCD inhibitor CVT-11127 For research use only.

CVT-11127

Synonyms: FMK13918

CVT-11127 (FMK13918) is an inhibitor of stearoylCoA desaturase-1 (SCD1). CVT-11127 reduces lipid synthesis and impaires proliferation by blocking the progression of cell cycle through the G(1)/S boundary and by triggering programmed cell death.

CVT-11127 Chemical Structure

CVT-11127 Chemical Structure

CAS: 1018674-83-3

Purity & Quality Control

Batch: S447001 DMSO] 6 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.96%
99.96

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Biological Activity

Description CVT-11127 (FMK13918) is an inhibitor of stearoylCoA desaturase-1 (SCD1). CVT-11127 reduces lipid synthesis and impaires proliferation by blocking the progression of cell cycle through the G(1)/S boundary and by triggering programmed cell death.
Targets
SCD1 [1]
In vitro
In vitro

CVT-11127, an SCD1 inhibitor, decreases expression of SCD1, prevents the growth of STK11/KEAP1 co-mutant cells and sensitizes those cells to ferroptosis induction.[2]
Cell Research Cell lines H358 isogenic clones (NTC, STK11KO, KEAP1KO, DKO)
Concentrations 1 μM
Incubation Time 4 days
Method

3000 cells per well of the indicated genotype were plated in 96 well plates in triplicate for each drug dose. Cells were allowed to attach to the plate overnight in RPMI media supplemented with 10% FBS. Drug titrations were performed in full serum media in a separate 96 well plate with column 11 containing the highest dose of the drug, column 3 containing the lowest dose of the drug, and column 2 containing no drug. Outside wells were filled with PBS to avoid liquid precipitation. After cells were allowed to attached to the plate overnight, media was aspirated using a multi-channel aspirator, and replaced with drug media from the drug titration plates. Cells were inclubated at 37 degrees for 3–4 days as indicated. On the final day of drug treatment, media was once again aspirated using a multi-channel aspirator. Cells were washed 1× with PBS then fixed to the plate and stained with a solution of 0.5% crystal violet, 1% Methanol, and 1% Formaldehyde in PBS for 15 minutes at room temperature.

Chemical Information & Solubility

Molecular Weight 512.39 Formula

C25H23Cl2N5O3
CAS No. 1018674-83-3 SDF --
Smiles COC1=CC=C(C=C1)C2=NC3=C(N=C(NCC4=CC(=C(Cl)C=C4)Cl)C=C3)N(CCNC(C)=O)C2=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 6 mg/mL ( (11.7 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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