(-)-Catechin gallate

Catalog No.E0239 Synonyms: (-)-CG

For research use only.

(-)-Catechin gallate ((-)-CG) is a catechin polyphenol that has been found in green tea extracts with diverse biological activities.

(-)-Catechin gallate Chemical Structure

CAS No. 130405-40-2

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Biological Activity

Description (-)-Catechin gallate ((-)-CG) is a catechin polyphenol that has been found in green tea extracts with diverse biological activities.
In vitro

(-)-Catechin gallate is a minor polyphenolic constituent in green tea, which induces apoptosis of cells derived from tissues of the human oral cavity by the more specific TUNEL procedure.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Immortalized epithelioid gingival S-G cells, salivary gland squamous carcinoma HSG cells, normal gingival HGF-1 fibroblasts, tongue squamous carcinoma CAL27 cells
  • Concentrations: 50, 100, 150, 200 µM
  • Incubation Time: 3 days
  • Method:

    Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 ml of the growth medium containing 2×10<sup>4</sup>cells/well for a 1-day exposure, 1.5×10<sup>4</sup>cells/ well for a 2-day exposure and 1×10<sup>4</sup>cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium is removed and replaced with exposure medium, with or without varied concentrations of the test agents. In some studies the cells are coexposed to CG and 100 Units/ml catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red (NR) assay.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 442.37
Formula

C22H18O10

CAS No. 130405-40-2
Storage 3 years -20°C powder
2 years -80°C in solvent

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