(-)-Catechin gallate

Catalog No.E0239 Batch:E023901

Technical Data

Formula	C₂₂H₁₈O₁₀
Molecular Weight	442.37	CAS No.	130405-40-2
Solubility (25°C)*	In vitro	DMSO	88 mg/mL (198.92 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	(-)-Catechin gallate ((-)-CG) is a catechin polyphenol that has been found in green tea extracts with diverse biological activities.
In vitro	(-)-Catechin gallate is a minor polyphenolic constituent in green tea, which induces apoptosis of cells derived from tissues of the human oral cavity by the more specific TUNEL procedure.^[1]

Protocol (from reference)

Cell Assay:^[1]	Cell lines Immortalized epithelioid gingival S-G cells, salivary gland squamous carcinoma HSG cells, normal gingival HGF-1 fibroblasts, tongue squamous carcinoma CAL27 cells Concentrations 50, 100, 150, 200 µM Incubation Time 3 days Method Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 ml of the growth medium containing 2×10<sup>4</sup>cells/well for a 1-day exposure, 1.5×10<sup>4</sup>cells/ well for a 2-day exposure and 1×10<sup>4</sup>cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium is removed and replaced with exposure medium, with or without varied concentrations of the test agents. In some studies the cells are coexposed to CG and 100 Units/ml catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red (NR) assay.

Cell Assay:^[1]

Cell lines
Immortalized epithelioid gingival S-G cells, salivary gland squamous carcinoma HSG cells, normal gingival HGF-1 fibroblasts, tongue squamous carcinoma CAL27 cells
Concentrations
50, 100, 150, 200 µM
Incubation Time
3 days
Method
Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 ml of the growth medium containing 2×104cells/well for a 1-day exposure, 1.5×104cells/ well for a 2-day exposure and 1×104cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium is removed and replaced with exposure medium, with or without varied concentrations of the test agents. In some studies the cells are coexposed to CG and 100 Units/ml catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red (NR) assay.

References

[1] Babich H, et al. Toxicol Lett. 2007 Jul 10;171(3):171-80.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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