Brassinolide

Catalog No.S3882

For research use only.

Brassinolide, a plant steroid isolated from Brassica napus pollen, promotes cell elongation and cell devision and thus regulates plant growth and development.

Brassinolide Chemical Structure

CAS No. 72962-43-7

Purity & Quality Control

Related Other Products

Biological Activity

Description Brassinolide, a plant steroid isolated from Brassica napus pollen, promotes cell elongation and cell devision and thus regulates plant growth and development.
In vitro

Brassinolide is a naturally occurring plant hormone that promotes growth, increases yields for grain and fruit crops, and makes plants more resistant to drought and cold weather. Brassinolide could induce a time and concentration-dependent cytotoxicity in PC-3 cells. Treatment with brassinolide triggers a time-dependent decrease in the expression of anti-apoptotic protein Bcl-2 and marked accumulation in G2/M phase of cell cycle[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: PC-3 cells
  • Concentrations: 0, 10, 20 and 40 μM
  • Incubation Time: 12, 24 and 36 h
  • Method:

    PC-3 cells are treated with various concentrations of brassinolide (0, 10, 20 and 40 μM) for 12, 24 and 36 h.

  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 96 mg/mL
(199.71 mM)
Ethanol 25 mg/mL
(52.0 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 480.68
Formula

C28H48O6

CAS No. 72962-43-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)C(C)C(C(C(C)C1CCC2C1(CCC3C2COC(=O)C4C3(CC(C(C4)O)O)C)C)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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