Bilibubin

Catalog No.S5116 Synonyms: Bilirubin

For research use only.

Bilibubin (Bilirubin) is a tetrapyrrole and a breakdown product of heme catabolism that acts as a physiological, chain-breaking antioxidant.

Bilibubin Chemical Structure

CAS No. 635-65-4

Purity & Quality Control

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Biological Activity

Description Bilibubin (Bilirubin) is a tetrapyrrole and a breakdown product of heme catabolism that acts as a physiological, chain-breaking antioxidant.
In vitro

As a potent antioxidant, bilirubin effectively blocks the generation of cellular reactive oxygen species induced by the cross-linking of endothelial vascular cell adhesion molecule 1 (VCAM‐1) or intercellular adhesion molecule 1 (ICAM-1)[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Human umbilical vein endothelial cells (HUVECs)
  • Concentrations: 20 μmol/L
  • Incubation Time: 24 h
  • Method: HUVEC monolayers are incubated with TNF-α (5 ng/mL) in the presence of bilirubin (BR; 20 μmol/L) or the bilirubin vehicle (Veh) for 24 hours, and protein levels of IL‐6, IL‐8, IL‐1β, MCP‐1, M‐CSF, and RANTES in cell-culture medium are determined.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: C57BL/6J mice
  • Dosages: 30 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 3 mg/mL
(5.13 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 584.66
Formula

C33H36N4O6

CAS No. 635-65-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(NC(=C1CCC(=O)O)CC2=C(C(=C(N2)C=C3C(=C(C(=O)N3)C)C=C)C)CCC(=O)O)C=C4C(=C(C(=O)N4)C=C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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