Berberine

Catalog No.S9046 Batch:S904602

Technical Data

Formula	C₂₀H₁₈NO₄
Molecular Weight	336.36	CAS No.	2086-83-1
Solubility (25°C)*	In vitro	DMSO	17 mg/mL (50.54 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Berberine (Umbellatine), an alkaloid isolated from Rhizoma coptidis, has broad applications, particularly as an antibacterial agent in the clinic.
In vitro	In 3T3-L1 adipocytes, the treatment of 12 μM or 20 μM berberine combined with 25 μM resveratrol shows more significant inhibition of lipid accumulation compared with individual compounds. The combination of berberine with resveratrol significantly increases the low-density-lipoprotein receptor expression in HepG2 cells to a level about one-fold higher in comparison to individual compounds.^[1]
In vivo	The combination of Berberine and resveratrol exerts a significant enhanced effect on lipid-lowering in hyperlipidemic mice and intracellular lipid accumulation-decreasing in adipocytes as compared to individual agent alone, which is closely associated with SIRT1 and LDLR.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines 3T3-L1 adipocytes, HepG2 cells, hepatic L02 cells Concentrations 12 μM, 20 μM, 50 μM Incubation Time 3 days, 24 h Method At day 10 of differentiation, 3T3-L1 adipocytes are treated with Berberine, resveratrol, and their combination for 3 days. The intracellular lipid accumulation of differentiated cells is detected by oil red O staining. HepG2 cells are cultured in 6-well plate for 24 h with a 3 × 10₅ cell density, and then the culture medium is replaced by fresh medium containing Berberine, resveratrol, and their combination for another 24 h. Subsequently, the total proteins from the cells are extracted for assaying the low-density-lipoprotein receptor (LDLR) expression by Western Blot.

Cell Assay:^{^[1]}

Cell lines
3T3-L1 adipocytes, HepG2 cells, hepatic L02 cells
Concentrations
12 μM, 20 μM, 50 μM
Incubation Time
3 days, 24 h
Method
At day 10 of differentiation, 3T3-L1 adipocytes are treated with Berberine, resveratrol, and their combination for 3 days. The intracellular lipid accumulation of differentiated cells is detected by oil red O staining. HepG2 cells are cultured in 6-well plate for 24 h with a 3 × 10₅ cell density, and then the culture medium is replaced by fresh medium containing Berberine, resveratrol, and their combination for another 24 h. Subsequently, the total proteins from the cells are extracted for assaying the low-density-lipoprotein receptor (LDLR) expression by Western Blot.

References

[1] Zhu X, et al. Int J Mol Sci. 2018 Dec 6;19(12):3903.

Selleck's Berberine has been cited by 11 publications

System analysis of Huang-Lian-Jie-Du-Tang and their key active ingredients for overcoming CML resistance by suppression of leukemia stem cells [ Phytomedicine, 2023, 117:154918]	PubMed: 37329755
Nao Tan Qing ameliorates Alzheimer's disease-like pathology by regulating glycolipid metabolism and neuroinflammation: A network pharmacology analysis and biological validation [ Pharmacol Res, 2022, 185:106489]	PubMed: 36228869
Vagus nerve stimulated by microbiota-derived hydrogen sulfide mediates the regulation of berberine on microglia in transient middle cerebral artery occlusion rats [ Phytother Res, 2022, 10.1002/ptr.7490]	PubMed: 35583808
Berberine Sensitizes Human Hepatoma Cells to Regorafenib via Modulating Expression of Circular RNAs [ Front Pharmacol, 2021, 12:632201]	PubMed: 34220494
Quantitative Proteomics Analysis of Berberine-Treated Colon Cancer Cells Reveals Potential Therapy Targets [ Biology (Basel), 2021, 10(3)250]	PubMed: 33806918
Berberine exerts neuroprotective activities against cerebral ischemia/reperfusion injury through up-regulating PPAR-γ to suppress NF-κB-mediated pyroptosis [ Brain Res Bull, 2021, 177:22-30]	PubMed: 34517069
Sensitivity of pancreatic cancer cells to chemotherapeutic drugs, signal transduction inhibitors and nutraceuticals can be regulated by WT-TP53 [ Adv Biol Regul, 2021, S2212-4926(20)30091-9]	PubMed: 33451973
Suppression effect of plant-derived berberine on cyprinid herpesvirus 2 proliferation and its pharmacokinetics in Crucian carp (Carassius auratus gibelio) [ Antiviral Res, 2020, 186:105000]	PubMed: 33359191
Berberine Inhibits Nod-Like Receptor Family Pyrin Domain Containing 3 Inflammasome Activation and Pyroptosis in Nonalcoholic Steatohepatitis via the ROS/TXNIP Axis. [ Front Pharmacol, 2020, 11:185]	PubMed: 32194416
Combination of Berberine with Resveratrol Improves the Lipid-Lowering Efficacy. [ Int J Mol Sci, 2018, 19(12)]	PubMed: 30563192

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SHIPPING AND STORAGE
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