Home Apoptosis PERK modulator Azoramide For research use only.

Azoramide

Azoramide is a small-molecule modulator of the unfolded protein response (UPR). It improves ER protein-folding ability and activates ER chaperone capacity to protect cells against ER stress.

Azoramide Chemical Structure

Azoramide Chemical Structure

CAS: 932986-18-0

Selleck's Azoramide has been cited by 3 publications

Purity & Quality Control

Batch: S830401 DMSO] 61 mg/mL] false] Ethanol] 61 mg/mL] false] Water] Insoluble] false Purity: 99.59%
99.59

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Biological Activity

Description Azoramide is a small-molecule modulator of the unfolded protein response (UPR). It improves ER protein-folding ability and activates ER chaperone capacity to protect cells against ER stress.
Targets
UPR [1]
In vitro
In vitro Azoramide may have the protective effects of enhancing chaperone expression and reducing protein synthesis without inducing cytotoxicity and apoptosis. Azoramide may require the presence of intact IRE1 and PERK branches of the UPR to fully increase chaperone capacity. Azoramide is found to be a kind of compound with the dual property of not only boosting ER folding acutely but also activating ER chaperone capacity chronically to promote ER homeostasis. Its treatment potently protects cells against chemically-induced ER stress conditions. Azoramide preserves beta cell function and survival during metabolic ER stress. Azoramide pretreatment does not impair ER function as part of its initial action. Azoramide treatment leads to increased SERCA expression, resulting in enhanced retention of Ca+2 within the ER. Azoramide interacts with UPR pathways to promote resolution of ER stress and improve ER function[1].
Cell Research Cell lines INS1 cells
Concentrations 20 µM
Incubation Time 60 h
Method

INS1 cells are treated with 25 mM Glucose and 500 µM Palmitate (G/P) in the absence or presence of 20 µM azoramide for 60 hours. At the end of the incubation, viability is measured using the CellTiter-Glo cell viability assay system.
In Vivo
In vivo Azoramide improves glucose homeostasis in mice with genetic obesity and diet-induced obesity. Remarkably, azoramide treatment significantly improves insulin sensitivity and glucose tolerance and beta cell function in obese mice in multiple preclinical models[1].
Animal Research Animal Models ob/ob mice
Dosages 150mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 308.83 Formula

C15H17ClN2OS
CAS No. 932986-18-0 SDF Download Azoramide SDF
Smiles CCCC(=O)NCCC1=CSC(=N1)C2=CC=C(C=C2)Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 61 mg/mL ( (197.51 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 61 mg/mL

Water : Insoluble


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In vivo
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