Azoramide

Catalog No.S8304 Batch:S830401

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Technical Data

Formula

C15H17ClN2OS
Molecular Weight 308.83 CAS No. 932986-18-0
Solubility (25°C)* In vitro DMSO 61 mg/mL (197.51 mM)
Ethanol 61 mg/mL (197.51 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO Corn oil
3.05mg/ml Taking the 1 mL working solution as an example, add 50 μL of 61 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Azoramide is a small-molecule modulator of the unfolded protein response (UPR). It improves ER protein-folding ability and activates ER chaperone capacity to protect cells against ER stress.
Targets
UPR [1]
In vitro Azoramide may have the protective effects of enhancing chaperone expression and reducing protein synthesis without inducing cytotoxicity and apoptosis. Azoramide may require the presence of intact IRE1 and PERK branches of the UPR to fully increase chaperone capacity. Azoramide is found to be a kind of compound with the dual property of not only boosting ER folding acutely but also activating ER chaperone capacity chronically to promote ER homeostasis. Its treatment potently protects cells against chemically-induced ER stress conditions. Azoramide preserves beta cell function and survival during metabolic ER stress. Azoramide pretreatment does not impair ER function as part of its initial action. Azoramide treatment leads to increased SERCA expression, resulting in enhanced retention of Ca+2 within the ER. Azoramide interacts with UPR pathways to promote resolution of ER stress and improve ER function[1].
In vivo Azoramide improves glucose homeostasis in mice with genetic obesity and diet-induced obesity. Remarkably, azoramide treatment significantly improves insulin sensitivity and glucose tolerance and beta cell function in obese mice in multiple preclinical models[1].

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    INS1 cells

  • Concentrations

    20 µM

  • Incubation Time

    60 h

  • Method

    INS1 cells are treated with 25 mM Glucose and 500 µM Palmitate (G/P) in the absence or presence of 20 µM azoramide for 60 hours. At the end of the incubation, viability is measured using the CellTiter-Glo cell viability assay system.
Animal Study:

[1]
  • Animal Models

    ob/ob mice

  • Dosages

    150mg/kg

  • Administration

    oral

Selleck's Azoramide has been cited by 3 publications

Azoramide ameliorates cadmium-induced cytotoxicity by inhibiting endoplasmic reticulum stress and suppressing oxidative stress [ PeerJ, 2024, 12:e16844] PubMed: 38313032
Exogenous iron impairs the anti-cancer effect of ascorbic acid both in vitro and in vivo [ J Adv Res, 2022, S2090-1232(22)00149-7] PubMed: 35777727
Azoramide ameliorated tachypacing-induced injury of atrial myocytes differentiated from human induced pluripotent stem cell by regulating endoplasmic reticulum stress [ Stem Cell Res, 2022, 60:102686] PubMed: 35101669

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.