Adavosertib (MK-1775)

Name: Adavosertib (MK-1775)
Brand: Selleck Chemicals
SKU: S1525
Rating: 5 (155 reviews)

For research use only.

Catalog No.S1525 Synonyms: AZD1775

155 publications

Adavosertib (MK-1775) Chemical Structure

CAS No. 955365-80-7

Adavosertib (MK-1775, AZD1775) is a potent and selective Wee1 inhibitor with IC50 of 5.2 nM in a cell-free assay; hinders G2 DNA damage checkpoint. Phase 2.

Selleck's Adavosertib (MK-1775) has been cited by 155 publications

Cancer Cell, 2020, 15 pii: S1535-6108(20)30164-1

PubMed: 32359397 ( click the link to review the publication )

Nat Med, 2019, 25(5):838-849

PubMed: 31011202 ( click the link to review the publication )

Cancer Cell, 2019, 35(3):519-533

PubMed: 30889383 ( click the link to review the publication )

Cancer Cell, 2019, 35(5):752-766

PubMed: 31085176 ( click the link to review the publication )

Cell, 2018, 173(1):104-116

PubMed: 29502971 ( click the link to review the publication )

Cell, 2013, 155(5):1088-103

PubMed: 24267891 ( click the link to review the publication )

Sci Adv, 2021, 7(7)eabd2645

PubMed: 33579708 ( click the link to review the publication )

Neuro Oncol, 2021, noab197

PubMed: 34383057 ( click the link to review the publication )

Leukemia, 2021, 10.1038/s41375-021-01361-8

PubMed: 34341479 ( click the link to review the publication )

Cell Rep, 2021, 34(11):108870

PubMed: 33730585 ( click the link to review the publication )

Cell Rep, 2021, 34(9):108808

PubMed: 33657372 ( click the link to review the publication )

Mol Cancer, 2021, 20(1):97

PubMed: 34315482 ( click the link to review the publication )

Haematologica, 2021, 10.3324/haematol.2021.278743

PubMed: 34162179 ( click the link to review the publication )

Cancers (Basel), 2021, 13(14)3487

PubMed: 34298699 ( click the link to review the publication )

Cell Death Dis, 2021, 12(7):683

PubMed: 34234118 ( click the link to review the publication )

Br J Cancer, 2021, 10.1038/s41416-021-01441-7

PubMed: 34088988 ( click the link to review the publication )

Endocr Relat Cancer, 2021, 28(5):311-324

PubMed: 33769310 ( click the link to review the publication )

Int J Mol Sci, 2021, 22(13)6943

PubMed: 34203295 ( click the link to review the publication )

FEBS J, 2021, 10.1111/febs.15747

PubMed: 33529438 ( click the link to review the publication )

Front Oncol, 2021, 11:681530

PubMed: 34277427 ( click the link to review the publication )

Front Oncol, 2021, 11:683688

PubMed: 34354944 ( click the link to review the publication )

Biochem Pharmacol, 2021, 188:114494

PubMed: 33684390 ( click the link to review the publication )

Int J Biochem Cell Biol, 2021, 137:106028

PubMed: 34171479 ( click the link to review the publication )

Cancer Res Treat, 2021, 10.4143/crt.2021.320

PubMed: 33971703 ( click the link to review the publication )
Cell Death Differ, 2021, 10.1038/s41418-020-00733-4

PubMed: 33531658 ( click the link to review the publication )

Life Sci Alliance, 2021, 4(3)e202000980

PubMed: 33402344 ( click the link to review the publication )

Int J Radiat Oncol Biol Phys, 2021, S0360-3016(21)00361-8

PubMed: 34112559 ( click the link to review the publication )

Sci Adv, 2020, 6(24):eaaz7808

PubMed: 32577513 ( click the link to review the publication )

Clin Cancer Res, 2020, 27 pii: clincanres

PubMed: 32341033 ( click the link to review the publication )

Cell Rep, 2020, 33(9):108449

PubMed: 33264622 ( click the link to review the publication )

Haematologica, 2020, 12;haematol.2019.233445

PubMed: 32165486 ( click the link to review the publication )

Cancer Lett, 2020, 472:119-131

PubMed: 31866466 ( click the link to review the publication )

Ther Adv Med Oncol, 2020, 12:1758835920974201

PubMed: 33425022 ( click the link to review the publication )

Mol Oncol, 2020, 10.1002/1878-0261.12882

PubMed: 33320980 ( click the link to review the publication )

Br J Haematol, 2020, 21

PubMed: 32314355 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(1):148-163

PubMed: 32064158 ( click the link to review the publication )

Am J Cancer Res, 2020, 1;10(3):908-924

PubMed: 32266099 ( click the link to review the publication )

Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0507

PubMed: 32229503 ( click the link to review the publication )

J Cell Mol Med, 2020, 27

PubMed: 32459381 ( click the link to review the publication )

Int J Mol Sci, 2020, 18;21(8):2825

PubMed: 32325639 ( click the link to review the publication )

Cancer Cell Int, 2020, 20:253

PubMed: 32565737 ( click the link to review the publication )

Ther Adv Hematol, 2020, 11:2040620719898373

PubMed: 32010435 ( click the link to review the publication )

Diagnostics (Basel), 2020, 10(2)

PubMed: 32098452 ( click the link to review the publication )

Cell Rep, 2020, 32(2):107901

PubMed: 32668239 ( click the link to review the publication )

JCI Insight, 2020, 143474

PubMed: 33320833 ( click the link to review the publication )

J Neurooncol, 2020, 10.1007

PubMed: 32180106 ( click the link to review the publication )

Cancers (Basel), 2020, 12(6):1503

PubMed: 32526907 ( click the link to review the publication )

FEBS J, 2020, 10.1111/febs.15280

PubMed: 32144855 ( click the link to review the publication )
Nat Commun, 2019, 10(1):2189

PubMed: 31097698 ( click the link to review the publication )

Clin Cancer Res, 2019, 10.1158/1078-0432.CCR-19-0775

PubMed: 31806643 ( click the link to review the publication )

Clin Cancer Res, 2019, 25(11):3430-3442

PubMed: 30755439 ( click the link to review the publication )

Leukemia, 2019, 10.1038/s41375-019-0456-2

PubMed: 30967619 ( click the link to review the publication )

Haematologica, 2019, 104(11):2225-2240

PubMed: 30819918 ( click the link to review the publication )

Clin Cancer Res, 2019, 25(7):2290-2304

PubMed: 30538111 ( click the link to review the publication )

J Cell Biol, 2019, 218(5):1531-1552

PubMed: 30858191 ( click the link to review the publication )

Cancer Res, 2019, 10.1158/0008-5472.CAN-18-3374

PubMed: 31551363 ( click the link to review the publication )

Elife, 2019, e46902

PubMed: 31264963 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):463

PubMed: 31718704 ( click the link to review the publication )

Cancers (Basel), 2019, 11(11)

PubMed: 31703356 ( click the link to review the publication )

Cancers (Basel), 2019, 11(6)

PubMed: 31200459 ( click the link to review the publication )

Cells, 2019, 8(2)

PubMed: 30754676 ( click the link to review the publication )

Cell Chem Biol, 2019, 10.1016/j.chembiol.2019.10.013

PubMed: 31735695 ( click the link to review the publication )

Oncoimmunology, 2019, 8(11):e1638207

PubMed: 31646086 ( click the link to review the publication )

Mol Cancer Ther, 2019, 18(8):1439-1450

PubMed: 31142662 ( click the link to review the publication )

Mol Cancer Ther, 2019, 10.1158/1535-7163.MCT-19-0474

PubMed: 31597711 ( click the link to review the publication )

Gastric Cancer, 2019, 10.1007/s10120-019-00977-1

PubMed: 31197522 ( click the link to review the publication )

Hum Mol Genet, 2019, 28(5):842-857

PubMed: 30445628 ( click the link to review the publication )

Eur J Med Chem, 2019, 161:479-492

PubMed: 30388464 ( click the link to review the publication )

J Virol, 2019, 93(14)

PubMed: 31043526 ( click the link to review the publication )

Sci Rep, 2019,

PubMed: 31848373 ( click the link to review the publication )

Sci Rep, 2019, 9(1):6161

PubMed: 30992462 ( click the link to review the publication )

Int J Biol Sci, 2019, 15(9):1942-1954

PubMed: 31523195 ( click the link to review the publication )
Front Pharmacol, 2019, 10:864

PubMed: 31427973 ( click the link to review the publication )

Exp Cell Res, 2019, 111720

PubMed: 31738907 ( click the link to review the publication )

PLoS One, 2019, 14(2):e0212210

PubMed: 30779812 ( click the link to review the publication )

PLoS One, 2019, 14(11):e0221288

PubMed: 31721781 ( click the link to review the publication )

Biochem Biophys Res Commun, 2019, 519(3):597-604

PubMed: 31540690 ( click the link to review the publication )

Biochem Biophys Res Commun, 2019, 10.1016/j.bbrc.2019.10.124

PubMed: 31640856 ( click the link to review the publication )

Blood Adv, 2019, 3(7):1185-1196

PubMed: 30967394 ( click the link to review the publication )

Leukemia, 2019, 10.1038/s41375-019-0677-4

PubMed: 31836849 ( click the link to review the publication )

Nat Commun, 2018, 9(1):3982

PubMed: 30266942 ( click the link to review the publication )

Nucleic Acids Res, 2018, 46(10):5109-5124

PubMed: 29850896 ( click the link to review the publication )

J Immunother Cancer, 2018, 6(1):59

PubMed: 29925431 ( click the link to review the publication )

Cancer Res, 2018, 78(11):3054-3066

PubMed: 29735549 ( click the link to review the publication )

Cell Rep, 2018, 22(12):3206-3216

PubMed: 29562177 ( click the link to review the publication )

Cancer Lett, 2018, 430:34-46

PubMed: 29753759 ( click the link to review the publication )

J Exp Clin Cancer Res, 2018, 37(1):129

PubMed: 29954437 ( click the link to review the publication )

Cancers (Basel), 2018, 10(5)

PubMed: 29783721 ( click the link to review the publication )

Curr Biol, 2018, 28(23):3824-3832

PubMed: 30449668 ( click the link to review the publication )

Cells, 2018, 7(9)

PubMed: 30200644 ( click the link to review the publication )

Front Oncol, 2018, 8:143

PubMed: 29774202 ( click the link to review the publication )

Sci Rep, 2018, 8(1):948

PubMed: 29343688 ( click the link to review the publication )

Int J Biol Sci, 2018, 14(10):1221-1231

PubMed: 30123071 ( click the link to review the publication )

BMC Cancer, 2018, 18(1):922

PubMed: 30253737 ( click the link to review the publication )

Bioorg Med Chem, 2018, 26(14):4014-4024

PubMed: 29941193 ( click the link to review the publication )

PLoS One, 2018, 13(2):e0193098

PubMed: 29489886 ( click the link to review the publication )
Biomed Res Int, 2018, 2018:5813292

PubMed: 29977914 ( click the link to review the publication )

Oncol Lett, 2018, 16(4):5473-5481

PubMed: 30250620 ( click the link to review the publication )

SSRN, 2018, 10.2139/ssrn.3188455

PubMed: None ( click the link to review the publication )

Blood, 2017, 130(24):2631-2641

PubMed: 29018079 ( click the link to review the publication )

Genes Dev, 2017, 31(3):318-332

PubMed: 28242626 ( click the link to review the publication )

Aging Cell, 2017, 16(3):575-584

PubMed: 28345297 ( click the link to review the publication )

J Hematol Oncol, 2017, 10(1):77

PubMed: 28356161 ( click the link to review the publication )

J Med Chem, 2017, 60(18):7863-7875

PubMed: 28792760 ( click the link to review the publication )

Br J Cancer, 2017, 117(1):113-123

PubMed: 28535155 ( click the link to review the publication )

Mol Cancer Ther, 2017, 16(9):2022-2034

PubMed: 28619759 ( click the link to review the publication )

J Chromatogr B Analyt Technol Biomed Life Sci, 2017, 1044-1045:70-76

PubMed: 28088043 ( click the link to review the publication )

Sci Rep, 2017, 7:43517

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Oncotarget, 2017, 8(43):73705-73722

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Oncotarget, 2017, 8(7):10966-10979

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Oncotarget, 2017, 8(63):106352-106368

PubMed: 29290954 ( click the link to review the publication )

Oncotarget, 2017, 8(55):94780-94792

PubMed: 29212266 ( click the link to review the publication )

Oncotarget, 2017, 8(27):44295-44311

PubMed: 28574828 ( click the link to review the publication )

Oncotarget, 2017, 8(67):111026-111040

PubMed: 29340034 ( click the link to review the publication )

Oncotarget, 2017, 8(35):59476-59491

PubMed: 28938651 ( click the link to review the publication )

Oncotarget, 2017, 8(63):106352-106368

PubMed: 29290954 ( click the link to review the publication )

Clin Cancer Res, 2016, 22(17):4440-51

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Stem Cells, 2016, 34(7):1765-75

PubMed: 26946228 ( click the link to review the publication )

Mol Cancer Ther, 2016, 15(7):1669-81

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Mol Cancer Ther, 2016, 15(6):1332-43

PubMed: 27196784 ( click the link to review the publication )
J Cell Sci, 2016, 129(16):3167-77

PubMed: 27383768 ( click the link to review the publication )

Sci Rep, 2016, 6:33323

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BMC Cancer, 2016, 16:678

PubMed: 27558154 ( click the link to review the publication )

Oncotarget, 2016, 7(27):41320-41335

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Oncotarget, 2016, 7(31):49902-49916

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Oncotarget, 2016, 7(23):34785-99

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Genetics, 2016, 204(2):807-819

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Tumour Biol, 2016, 10.1007/s13277-016-5081-3

PubMed: 27220319 ( click the link to review the publication )

Genes Dev, 2015, 29(5):555-66

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Sci Rep, 2015, 5:16991

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Cancer Biol Ther, 2015, 16(12):1784-93

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Cell Cycle, 2015, 14(7):1070-81

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BMC Cancer, 2015, 15:462

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Pediatr Blood Cancer, 2015, 62(1):52-9

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Radiat Res, 2015, 184(6):660-9

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Oncotarget, 2015, 6(15):13072-87

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Genes Cancer, 2015, 6(5-6):184-213

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Oncotarget, 2015, 6(25):21074-84

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BMC Cancer, 2015, 15:462

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J Thorac Oncol, 2014, 9(7):965-73

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Genes Dev, 2014, 28(14):1578-91

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Clin Cancer Res, 2014, 20(16):4274-88

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J Cell Biol, 2014, 206(7):843-53

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J Hematol Oncol, 2014, 7(1):53

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Cancer Lett, 2014, 356(2 Pt B):656-68

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J Cell Mol Med, 2014, 18(1):143-55

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Cell Cycle, 2014, 13(2):303-14

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Pediatr Blood Cancer, 2014, 61(10):1767-73

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Oncotarget, 2014, 5(21):10546-57

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Cell, 2014, 157(3):565-79

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Expert Opin Drug Metab Toxicol, 2013, 9(2):215-24

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PLoS One, 2013, 8(3):e57523

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Obstet Gynecol Int, 2013, 2013:828165

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Purity & Quality Control

Choose Selective Wee1 Inhibitors

Biological Activity

Description

Adavosertib (MK-1775, AZD1775) is a potent and selective Wee1 inhibitor with IC50 of 5.2 nM in a cell-free assay; hinders G2 DNA damage checkpoint. Phase 2.

Features

The first reported Wee1 inhibitor.

Targets

Wee1 ^[1]
(Cell-free assay)

5.2 nM

In vitro

MK-1775 inhibits Wee1 kinase in an ATP-competitive manner. Compared to Wee1, MK-1775 displays 2- to 3-fold less potency against Yes with IC50 of 14 nM, 10-fold less potency against seven other kinases with >80% inhibition at 1 μM, and >100-fold selectivity over human Myt 1, another kinase that inhibits cyclin-dependent kinase 1 (CDC2) by phosphorylation at an alternative site (Thr14). By abrogating the DNA damage checkpoint via blockade of Wee1 activity in WiDr cells bearing mutated p53, MK-1775 treatment inhibits the basal phosphorylation of CDC2 at Tyr15 (CDC2Y15) with EC50 of 49 nM, and suppresses gemcitabine-, carboplatin- or cisplatin-induced phosphorylation of CDC2 and cell cycle arrest in a dose-dependent manner, with EC50 of 82 nM and 81 nM, 180 nM and 163 nM, as well as 159 nM and 160 nM, respectively. MK-1775 treatment alone at 30-100 nM has no significant antiproliferative effect in WiDr and H1299 cells, whereas MK-1775 at 300 nM, sufficient to inhibit Wee1 by >80%, displays moderate but significant antiproliferative effects by 34.1% in WiDr cells and 28.4% in H1299 cells. ^[1]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
ASPC-1	NFXaXnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NUPjbmVWUUN3ME2xN{4zKMLzIEGuNUDPxE1?	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR3OEm1OEc,OjV2NUi5OVQ9N2F-
BxPC-3	NYK0XGl3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M37kbmlEPTB;MD64JOKyKDBwMEOg{txO	M1z2PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW4PVU1Lz5{NUS1PFk2PDxxYU6=
CFPAC-1	NXzPRVlST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NYLmeGZ4UUN3ME2zMlMhyrFiMD6yJO69VQ>?	M1HWbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW4PVU1Lz5{NUS1PFk2PDxxYU6=
HPAC	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NFnRdXFKSzVyPUCuOUDDuSByLkCxJO69VQ>?	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR3OEm1OEc,OjV2NUi5OVQ9N2F-
MIAPaCa-2	NWS4bYNUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1Lze2lEPTB;MD61JOKyKDBwMEWg{txO	M3XvRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEW4PVU1Lz5{NUS1PFk2PDxxYU6=
PANC-1	M{jRcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3zESGlEPTB;MUCuOkDDuSBzLkGg{txO	MoHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NUi5OVQoRjJ3NEW4PVU1RC:jPh?=
SK-N-BE (2)	MknPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NVnZPJZNUUN3ME2yMlTjiIoEsfMAjVAvOyEQvF2=	M{\TVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
SK-N-BE (2), PAN→MK	NEOzZWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVXJR\|UxRTJ4LkdihKnDueLCiUmuOkDPxE1?	NH\DO\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), MK→PAN	Mn;pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NVPNTlBbUUN3ME2yMlTjiIoEsfMAjVAvOyEQvF2=	NHPRTYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-AS	M1vycmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NWXCN2JrUUN3ME2wMlUx6oDLwsJihKkxNjB{IN88US=>	MkPWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV\|MEi5NVYoRjJ3M{C4PVE3RC:jPh?=
SK-N-DZ	NXi3eXhxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M{DaNmlEPTB;MD6zOwKBkcLz4pEJNE4xOSEQvF2=	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV\|MEi5NVY9N2F-
SK-N-AS	NYfLb4pjSXCxcITvd4l{KEG\|c3H5	NUHsVJI4PTByIH7N	NInmdWU1QCCq	MW\pcoR2[2W\|IHPlcIwh[XCxcITvd4l{	M3nPbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
SK-N-DZ	NYO5cGRESXCxcITvd4l{KEG\|c3H5	NX2xdoJkPTByIH7N	NV;UVlB2PDhiaB?=	MorwbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyOEmxOkc,OjV\|MEi5NVY9N2F-
THP-1	MmLsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXHvbppiOTJ3L{K1NE82ODBibl2=	NHTUOmM1QCCq	NF76UldqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>?	NXX1T4Y5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPFQ3OTRpPkK1NFg1PjF2PD;hQi=>
MV4-11	NHS3NVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWP3RXJpOTJ3L{K1NE82ODBibl2=	MXe0PEBp	Mk[5bY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	NWX3e3BKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPFQ3OTRpPkK1NFg1PjF2PD;hQi=>
U937	M1rIW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NUTYSmtUOTJ3L{K1NE82ODBibl2=	MmPrOFghcA>?	NEDUVXFqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IH3hco5meg>?	NFzYUFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC4OFYyPCd-MkWwPFQ3OTR:L3G+
HL-60	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1nlelEzPS9{NUCvOVAxKG6P	NYS1UWZQPDhiaB?=	Mor0bY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	NIHINYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC4OFYyPCd-MkWwPFQ3OTR:L3G+
OCI-AML3	NH;KPWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXqxNlUwOjVyL{WwNEBvVQ>?	M1TnflQ5KGh?	MlTSbY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	NGrRW449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC4OFYyPCd-MkWwPFQ3OTR:L3G+
MOLM-13	NWi2WHd6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVqxNlUwOjVyL{WwNEBvVQ>?	NGXI[2U1QCCq	MY\pcoNz\WG\|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44u\GWyZX7k[Y51KG2jbn7ldi=>	MnfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOES2NVQoRjJ3MEi0OlE1RC:jPh?=
CMK	Mn7VR4VtdCCYaXHibYxqfHliQYPzZZk>	MkLMNVAuOTByMECgcm0>	MoTmO\|IhcA>?	NYnJd41temWmdXPld{Bk\WyuII\pZYxq[mm2eTDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nch?=	MkfaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkKzN\|EoRjJ2OU[yN\|MyRC:jPh?=
CMY	NYrj[G5FS2WubDDWbYFjcWyrdImgRZN{[Xl?	MkDXNVAuOTByMECgcm0>	NIO3[o44OiCq	MXPy[YR2[2W\|IHPlcIwhfmmjbHnibZR6KGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz	NFzpOVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NlM{OSd-MkS5OlI{OzF:L3G+
Dayo	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MYjJR\|UxRTF3MDDuUS=>	MlnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NkG5NVAoRjJ2Nk[xPVExRC:jPh?=
UW228	M3jSXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NWnzNmVbUUN3ME2yN\|Ihdk1?	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ4MUmxNEc,OjR4NkG5NVA9N2F-
IST-MES1	NYTOTpczS2WubDDWbYFjcWyrdImgRZN{[Xl?	NFrENIwyPTBxMkWwJI5O	Mn3rO\|IhcA>?	NX\GZohC\W6qYX7j[ZMhfGinIHPpd5Bt[XSrbjDjfZRwfG:6aXOg[YZn\WO2IHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{	NUnuO\|FlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlU4QDJpPkK0N\|Y2Pzh{PD;hQi=>
IST-MES2	NUjnS3FPS2WubDDWbYFjcWyrdImgRZN{[Xl?	M2DBSVE2OC9{NUCgcm0>	M2nDTlczKGh?	NXOzdVVw\W6qYX7j[ZMhfGinIHPpd5Bt[XSrbjDjfZRwfG:6aXOg[YZn\WO2IHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{	NV[yNXpGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlU4QDJpPkK0N\|Y2Pzh{PD;hQi=>
REN	M2\0O2NmdGxiVnnhZoltcXS7IFHzd4F6	NF33OlQyPTBxMkWwJI5O	NVTPclZjPzJiaB?=	MWPlcohidmOnczD0bIUh[2m\|cHzheIlvKGO7dH;0c5hq[yCnZn\lZ5QhcW5iYTDjc45k\W62cnH0bY9vNWSncHXu[IVvfCCvYX7u[ZI>	NUXXRZNnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlU4QDJpPkK0N\|Y2Pzh{PD;hQi=>
NCI-H2452	NYeyPXNJS2WubDDWbYFjcWyrdImgRZN{[Xl?	NFPNSocyPTBxMkWwJI5O	NHTuT3A4OiCq	NIDs[HlmdmijbnPld{B1cGViY3nzdIxifGmwIHP5eI91d3irYzDl[oZm[3RiaX6gZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDtZY5v\XJ?	NVyzdoJKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlU4QDJpPkK0N\|Y2Pzh{PD;hQi=>
MSTO-211H	MorXR4VtdCCYaXHibYxqfHliQYPzZZk>	MYmxOVAwOjVyIH7N	Mk\SO\|IhcA>?	MnG0[Y5p[W6lZYOgeIhmKGOrc4DsZZRqdiCleYTveI95cWNiZX\m[YN1KGmwIHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRibXHucoVz	NWqzdHdTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlU4QDJpPkK0N\|Y2Pzh{PD;hQi=>
NCI-H2052	M{fFeWNmdGxiVnnhZoltcXS7IFHzd4F6	NEm5cWwyPTBxMkWwJI5O	NVjJenhXPzJiaB?=	NWr6N41R\W6qYX7j[ZMhfGinIHPpd5Bt[XSrbjDjfZRwfG:6aXOg[YZn\WO2IHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{	MmLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NkW3PFIoRjJ2M{[1O\|gzRC:jPh?=
WEE1	NWnZZ4ZpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Moe4TWM2OD13LkKgcm0>	M{LhOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Nkm5OlU2Lz5{M{[5PVY2PTxxYU6=
CDC2	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NITqdYRKSzVy78{eNVAxOCCwTR?=	NX7OZ4FSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PVk3PTVpPkKzOlk6PjV3PD;hQi=>
CDK7	NI\iSHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MV\JR\|Ux97zgMUCwNEBvVQ>?	NG\YOlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[5PVY2PSd-MkO2PVk3PTV:L3G+
MYT1	NWLTWo5ST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MUXJR\|UxRTV\|MDDuUS=>	NFLOd4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[5PVY2PSd-MkO2PVk3PTV:L3G+
T98G	MW\BdI9xfG:\|aYOgRZN{[Xl?	Mo\3NVAxNzJ3MDDuUS=>	MUS2JIg>	NY\GS2RE\W6qYX7j[ZMhemGmaXH0bY9vNWmwZIXj[YQh[2WubDDrbYxtcW6p	MlO4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OUK3PVMoRjJzOUmyO\|k{RC:jPh?=
A549	NVf6[WI6SXCxcITvd4l{KEG\|c3H5	NVvHfodTOjByIH7N	NFi4THYyKGh?	MWPyZYRqd3OnboPpeIl7\XNiTmPDUGMh[2WubIOgbY4h[SCyNUOt[IVx\W6mZX70JI1idm6nch?=	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd7OUCzN{c,OjF5OUmwN\|M9N2F-
H460	MkDtRZBweHSxc3nzJGF{e2G7	MXSyNFAhdk1?	Moq3NUBp	NEnSbXlz[WSrb4PlcpNqfGm8ZYOgUnNEVENiY3XscJMhcW5iYTDwOVMu\GWyZX7k[Y51KG2jbn7ldi=>	NYjOWos6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3PVkxOzNpPkKxO\|k6ODN\|PD;hQi=>
H1299	M1K3dWFxd3C2b4Ppd{BCe3OjeR?=	NX7pSHUxOjByIH7N	NEH4WlcyKGh?	NX;TT4JNemGmaX;z[Y5{cXSrenXzJG5US0yFIHPlcIx{KGmwIHGgdFU{NWSncHXu[IVvfCCvYX7u[ZI>	NVHC[I9{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3PVkxOzNpPkKxO\|k6ODN\|PD;hQi=>
Calu-6	M4fWWWFxd3C2b4Ppd{BCe3OjeR?=	NU\LVmhrOjByIH7N	NVPzUGJDOSCq	NYfDSIZnemGmaX;z[Y5{cXSrenXzJG5US0yFIHPlcIx{KGmwIHGgdFU{NWSncHXu[IVvfCCvYX7u[ZI>	MnHuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5OUmwN\|MoRjJzN{m5NFM{RC:jPh?=
WiDr	M{PQOGtqdmG\|ZTDBd5NigXN?	NUXFNWU5OTBvMUCwNFAhdk1?	MYm4JIg>	M{THWolvcGmkaYTzJJBpd3OyaH;yfYxifGmxbjDv[kBETEN{IHH0JHR6ejF3IIfpeIgh[W5iRVO1NOKhfmGudXWgc4YhQDVibn3vcE9NKHC{ZYTy[YF1\WRid3n0bEBo\W2laYThZolv\Q>?	NHLvSoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi4O\|U1PSd-MUm4PFc2PDV:L3G+
Function assay	NHv4bVVGgHCrMkmzSi=>			MmLPRolv\GmwZzDh[oZqdmm2eTD0c{Bz\WOxbXLpcoFvfCCqdX3hckBnfWyuLXzlcod1cCCQLYTldo1qdmGuIFjpd\|gufGGpZ3XkJHdm\TFiKEGgeI8hPjR4IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4hcHWvYX6gSZhxcTJ7M1[gZ4VtdHNiYYPz[ZN{\WRiYYOg[IV{e2:laXH0bY9vKGOxboP0ZY51KGK7IIH1ZY51cXSjdHn2[UBz\WGuLYTpcYUhWEOUIH3leIhw\CxiS3SgQUAxNjByM{Kg{txONg>?	M1fWfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Function assay	NITWT\|BGgHCrMkmzSi=>			MVfCbY5lcW6pIHHm[olvcXS7IITvJJJm[2:vYnnuZY51KGi3bXHuJIZ2dGxvbHXu[5RpKE5vdHXycYlv[WxiSHnzPE11[WepZXSgW4VmOiBqMTD0c{A2PjdicnXzbYR2\XNrIHX4dJJme3OnZDDpckBpfW2jbjDFfJBqOjl\|RjDj[YxteyCjc4Pld5Nm\CCjczDk[ZN{d2OrYYTpc44h[2:wc4ThcpQh[nlicYXhcpRqfGG2aY\lJJJm[WxvdHnt[UBRS1JibXX0bI9lNCCNZDC9JFAvODB\|OTFOwG0v	M1r2U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Antiproliferative assay	NE\JU5JOTEFvTVKtNlMy		MmHXO\|IhcHK\|	NHH5NmFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNibXXhd5Vz\WRiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2EbIXlJIF{e2G7LDDJR\|UxKD1iMD6yOkDPxE1w	NGjqWXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe5Nlc3OCd-Mki3PVI4PjB:L3G+
Antiproliferative assay	MWHISWszQTOW		NHPJVIY4OiCqcoO=	NX\XbYMySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJTUt{OUPUJINmdGy\|IH3lZZN2emWmIHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvQnz1[UBie3OjeTygTWM2OCB;IECuNlkh\|ryPLh?=	M4S4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Antiproliferative assay	NXnWUpROVU1zUx?=		MmHXO\|IhcHK\|	NHvR[FNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3NNXMh[2WubIOgcYVie3W{ZXSgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncj3CcJVmKGG\|c3H5MEBKSzVyIE2gNE4{OSEQvF2u	MlfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OUK3OlAoRjJ6N{myO\|YxRC:jPh?=
Function assay	MVfISWszQTN?		NH\QXlAyKGi{	MVjJcohq[mm2aX;uJI9nKGi3bXHuJIZ2dGxibHXu[5RpKFCNTWnUNUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJJV{cW6pIFXGV{ApOjR5IITvJFI2QSC{ZYPp[JVmeyliYYOgd5Vje3S{YYTlJIFnfGW{IEGgbJIh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCrbX31co9ie2G7LDDLbUA:KDBwNEeg{txONg>?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl2MUG5N{c,Ojl7NEGxPVM9N2F-
Function assay	M33FNGhGUzJ7Mx?=		M4rMSlEhcHJ?	MYLJcohq[mm2aX;uJI9nKGi3bXHuJIZ2dGxibHXu[5RpKFCNTWnUNUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJJV{cW6pIFXGV{ApOjR5IITvJFI2QSC{ZYPp[JVmeyliYYOgd5Vje3S{YYTlJIFnfGW{IEGgbJIh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCrbX31co9ie2G7LDDJR\|UxKD1iND65OEDPxE1w	NYfqVnE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5OFEyQTNpPkK5PVQyOTl\|PD;hQi=>
Function assay	MmTlUWRCNU2ELUKzNS=>	MWSwMlEhfG9iMUCgeW0>	MX:2JIhzew>?	MU\Jcohq[mm2aX;uJI9nKFenZUGgbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCFRFuxJJBpd3OyaH;yfYxifGmxbjDheEBVgXJiMUWgZZQhOC5zIITvJFExKHWPIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	M2TuZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO\|YxLz5{OEe5Nlc3ODxxYU6=
Function assay	NXmzNYVqUEWNMkmzWC=>	MoPlNE4yKHSxIEGwJJVO	MorQOkBpenN?	MmqwTY5pcWKrdHnvckBw\iCZZXWxJIlvKEiHS{K5N3Qh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iQ1TLNUBxcG:\|cHjvdplt[XSrb36gZZQhXHm{MUWgZZQhOC5zIITvJFExKHWPIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R?	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd7Mke2NEc,Ojh5OUK3OlA9N2F-
Function assay	MVPISWszQTOW	MY[wMlEhfG9iMUCgeW0>	MUK2JIhzew>?	NETDUnJKdmirYnn0bY9vKG:oIGDMT\|EhcW5iSFXLNlk{XCClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDUR3RRKHCqb4PwbI9zgWyjdHnvckBifCByLkGgeI8hOTBidV2gZYZ1\XJiNjDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZB?=	MorJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OUK3OlAoRjJ6N{myO\|YxRC:jPh?=
Function assay	NXnsW2puVUSDLV3CMVI{	NHzNSowxNjFidH:gNVAhfU1?	M2jVSFYhcHK\|	MoXSTY5pcWKrdHnvckBw\iCSTFuxJIlvKGi3bXHuJG1FSS2PQj2yN{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCWQ2TQJJBpd3OyaH;yfYxifGmxbjDheEAxNjFidH:gNVAhfU1iYX\0[ZIhPiCqcoOgZpkhX2W\|dHXyckBjdG:2IH3leIhw\A>?	NYHpc2FIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3PVI4PjBpPkK4O\|kzPzZyPD;hQi=>
qHTS assay	M4TQdXREOzJ?			NWjQSXRqeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGTDN\|Ih[2WubIO=	NYHxZ2l1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	MWTVMVIhV1N?			NIjufZFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiVT2yJG9UKGOnbHzz	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	M33MN2E3PzN?			MYrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>?	MlHTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NVvVeZRKTEGRWR?=			MlLEdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKESDT2mgZ4VtdHN?	MmW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	M174cHNid3NvMh?=			NHLSb3FyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz	NV;ad3VnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	M2TBeGJVNTN5			M2nJOpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2zO{Bk\Wyucx?=	MnPvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	MVrSSC=>			MYDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM>	MlTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	MkDyV2suVi2VSB?=			NH6x[WVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=>	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	MWnCWE0yOg>?			M{XLOpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?=	NWrnfol{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	Mo\pUmIyPjR\|			MkTxdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?=	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	NHqybIJQUFNvNUC=			NHLaN\|ByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	NFXP[5FDXC1zMh?=			NITITYVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1zMjDj[Yxtew>?	NYTzZ20zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	MkTwSGFQYQ>?			MlfqdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgSGFQYSClZXzsdy=>	MkjIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NYT5[odHW0tvTj3TTC=>			MmfOdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2VSDDj[Yxtew>?	NHP5O3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	MXfSbFQy			NXf6VlJQeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGLoOFEh[2WubIO=	NH;Hb5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	M17G[2E3PzN?			NXPLRXBLeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSTZ5MzDj[Yxteyl?	NWTYZnRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	Mn74UWchPjNiKE[tWGchWil?			NFn3fnhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBOTyB4MzCoOk1VTyCUKTDj[Yxtew>?	NELhWFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	NWj1RnQ4XS1{IF;T			M3vmXZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHUuOiCRUzDj[Yxtew>?	M2jlXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	NF25XnZQUFNvNUC=			NEfUNo1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBQUFNvNUCgZ4VtdHN?	MnrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	MlntVog1OQ>?			MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFQyKGOnbHzz	NYLIbollRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	NGPlR5VTTA>?			MXjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSSEBk\Wyucx?=	NYnjRVM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	MVzTTk1ISk1{			MWfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?=	M3fLb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
qHTS assay	M2rIXnNMNU5vTVO=			NGPHb5ByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=>	NX6yZoxCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
qHTS assay	M13aTG5DNUWEY{G=			NUXxZXNYeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM>	NFPSZ\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	M1HwOGxCVi13			NFrVcWFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
qHTS assay	NFHuT2RTcDF6			NYnYUGpEeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGLoNVgh[2WubIO=	Mne3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
qHTS assay	NVS0Wm5bW0pvR1LNNi=>			M3;nZ5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNLNUeETUKgZ4VtdHN?	NGnoVGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
qHTS assay	NEjaOY1U[W:\|LUK=			MnXwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV4Fwey1{IHPlcIx{	MnP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-Cdk1(Y15) / Cdk1; PubMed: 25609063 Clin Cancer Res The impact of a 24 hour pre-treatment of MK-1775 on p-Cdk1 was assessed by western blotting. Because of low basal phosphorylation, GBM22 was irradiated (RT) with 10 Gy. p-KAP1(S824) / p-Chk2(T68) / p-Chk1(S345); PubMed: 25609063 Clin Cancer Res Western blot evaluation of GBM6 and GBM22 short-term explant cultures 24 hours after treatment with either MK-1775 or TMZ or the combination. PARP / CF-PARP / pH3(S10) / p-CDC25c(S216) / p-CDK2(Y15); PubMed: 25458954 Cancer Lett Pancreatic cancer cells were treated with vehicle control or 500 nM MK-1775 for 48 h. Whole cell lysates were subjected to Western blotting and probed with anti-PARP, -p-H3, -γH2AX, -p-CHK1, -CHK1, -p-CDC25C, -p-CDK1, - CDK1, -p-CDK2, -CDK2, or -β-actin antibody. WEE1; PubMed: 27616351 Sci Rep MIA PaCa2, PANC-1, Hs 766T, Capan-1 and PL11 cells were treated with MK-1775 (400 nM/L) and/or MMC (150 nM/L) for 24 hours. Whole cell lysates were prepared using RIPA and western blot was performed to assess the protein expression of WEE1 (90 kDa), pCDK1(y15) (34 kDa), CDK1 (34 kDa) and GAPDH (36 kDa) as loading control.	25609063 25458954 27616351
Immunofluorescence	tubulin / p-HH3(S10); PubMed: 30755439 Clin Cancer Res FaDu and UNC7 cells were treated with adavosertib (500 nM), alisertib (250 nM), or adavosertib + alisertib for 24 hours and followed by immunofluorescent staining with anti-tubulin (Green) and anti-pHH3 (S10; Red). Nucleus was stained with DAPI. (A) Representative images of mitotic cells were captured by confocal microscopy. Scale bar: 10 µm. γH2AX; PubMed: 25609063 Clin Cancer Res A) γH2AX foci formation in GBM6 and GBM22 were assessed 24 h after a single treatment of 300 nM MK-1775. Cleaved caspase-3 / pH3; PubMed: 27616351 Sci Rep MIA PaCa2 cells were treated and dual stained with pH3 to observe mitotic entry; and cleaved caspase 3 (CL-CSP3) to observe caspase 3 activity.	30755439 25609063 27616351
Growth inhibition assay	Cell viability; PubMed: 25458954 Cancer Lett Pancreatic cancer cell lines were cultured in 96-well plates at 37℃ for 48 h in complete medium with variable concentrations of MK-1775 and viable cell numbers were determined using MTT reagent and a microplate reader. The data are presented as means ± standard errors from at least 3 independent experiments. IC50; PubMed: 25084614 J Hematol Oncol AML cell lines were cultured for 72 h in complete medium with variable concentrations of MK-1775 (MK) and viable cell numbers were determined using MTT assays. IC50 values were calculated as drug concentration necessary to inhibit 50% growth compared to untreated control cells.	25458954 25084614

In vivo

MK-1775 treatment alone at ~20 mg/kg displays minimal antitumor effects against WiDr xenografts in rats with T/C of 69% at day 3. Antitumor efficacy by MK-1775 alone in the nude rat HeLa-luc and TOV21G-shp53 xenograft models is also moderate. ^[1]

Protocol

Kinase Assay: ^[1]	- Collapse In vitro kinase assays: Recombinant human Wee1 is used. Kinase reaction is conducted with 10 μM ATP, 1.0 μCi of [γ-³³P]ATP, and 2.5 μg of poly(Lys, Tyr) as a substrate in the presence of increasing concentrations of MK-1775 at 30°C for 30 minutes. Radioactivity incorporated into the substrate is trapped on MultiScreen-PH plates and is counted on a liquid scintillation counter.
Cell Research: ^[1]	- Collapse Cell lines: WiDr, NCI-H1299, TOV21G, and HeLa Concentrations: Dissolved in DMSO, final concentrations ~10 μM Incubation Time: 24 hours Method: Cells are treated with or without gemcitabine for 24 hours, then with MK-1775 for an additional 24 hours. Cell viability is determined with a WST-8 kit using SpectraMax. Cellular caspase-3/7 activities are determined with a Caspase-3/7 Glo kit. (Only for Reference)
Animal Research: ^[1]	- Collapse Animal Models: Immunodeficient nude rats (F344/NJcl-rnu) bearing WiDr, HeLa-luc, or TOV21G-shp53 tumors Dosages: ~20 mg/kg/day Administration: Orally (Only for Reference)

References

[1] Hirai H, et al. Mol Cancer Ther, 2009, 8(11), 2992-3000.

Solubility (25°C)

In vitro	DMSO	80 mg/mL (159.8 mM)
	Water	0.0001 mg/mL (0.0 mM)
	Ethanol	''10 mg/mL warmed
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing.	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Adavosertib (MK-1775) SDF

Molecular Weight	500.6
Formula	C₂₇H₃₂N₈O₂
CAS No.	955365-80-7
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	AZD1775
Smiles	CC(C)(C1=NC(=CC=C1)N2C3=NC(=NC=C3C(=O)N2CC=C)NC4=CC=C(C=C4)N5CCN(CC5)C)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
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Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03668340	Recruiting	Drug: AZD1775	Uterine Cancer	Dana-Farber Cancer Institute\|AstraZeneca	October 22 2018	Phase 2
NCT03028766	Completed	Drug: AZD1775\|Drug: Cisplatin\|Radiation: Radiotherapy	Hypopharynx Squamous Cell Carcinoma\|Oral Cavity Squamous Cell Carcinoma\|Larynx Cancer	University of Birmingham\|AstraZeneca\|Cancer Research UK	June 22 2017	Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
How to prepare MK1775 methylcellulose solution? and how to prepare methylcellulose itself? Once make the MK1775 methylcellulose solution, how should i keep it?
Answer:
MK1775 in 0.5% methylcellulose is a suspension or emulsion, and it is ok to treat mice orally. It is recommended to dissolve methylcellulose in saline. It will take some time to dissolve methylcellulose, and you can vortex it for a while. The MK1775 methylcellulose solution can be stored at 4°C for a week.

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Adavosertib (MK-1775)