Cell-free Assays (IC50/Ki) for Adavosertib (AZD1775, MK-1775)

Key TargetIC50 (Cell-free)DescriptionReference
Wee1 kinase 5.2 nmol/L in vitro kinase assays Download PDF
pCDK1 240 nmol/L non-clinical data PMC10345044

Cell-based Assay (IC50) for Adavosertib (AZD1775, MK-1775)

Cell TypeIC50 (Cell-based)DescriptionReference
CAMA-1 504.2 nM CellTiterGlo Chemiluminescent kit Download PDF
IGROV1 306 ± 4 nM colony-forming assays Download PDF
SKOV3ip 133 ± 4 nM colony-forming assays Download PDF
DKMGvIII−, DKMGvIII+, BS153vIII−, BS153vIII+ approximately 400 nM Cell proliferation analysis (cells counted with Beckmann-Coulter) Download PDF
WM45.1 0.4 μM MTS assay PMC4460948
Check the Adavosertib (AZD1775, MK-1775) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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