Gefitinib (ZD1839)

For research use only.

Catalog No.S1025 Synonyms: Iressa

443 publications

Gefitinib (ZD1839) Chemical Structure

CAS No. 184475-35-2

Gefitinib (ZD-1839, Iressa) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively. Gefitinib promotes autophagy and apoptosis of lung cancer cells via blockade of the PI3K/AKT/mTOR pathway.

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Selleck's Gefitinib (ZD1839) has been cited by 443 publications

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Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839, Iressa) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively. Gefitinib promotes autophagy and apoptosis of lung cancer cells via blockade of the PI3K/AKT/mTOR pathway.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M4rGUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwMEW0JO69VQ>? M3n1XVE3PTF4NEez
NIH3T3 MVXGeY5kfGmxbjDBd5NigQ>? MVuwMlIh|ryP MljWNkBp NFvpeVBFVVOR MWPJcohq[mm2aX;uJI9nKEWJRjDzeIlufWyjdHXkJIh2dWGwIHXyZmIyKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODF2NDFOwG0> NX3qS4FzOTZ2OECyPFQ>
COS7 NYnmeINjTnWwY4Tpc44hSXO|YYm= MlPHPVYh|ryP NUTpcFZjUW6qaXLpeIlwdiCxZjDoeY1idiCMQVuyJHY3OTeIIH31eIFvfCCneIDy[ZN{\WR? NITmV2QyPzF5OEeyNi=>
A549 M4fPO2N6fG:2b4jpZ{BCe3OjeR?= NVfZfmhFQTZiaB?= M3z1ZmROW09? NWDQV2NkUUN3ME2xN{42QSEQvF2= Ml7SNVg{OTN6MEe=
K562 NV\uT|ZqS3m2b4TvfIlkKEG|c3H5 MVm5OkBp MXvEUXNQ M4TKZWlEPTB;OT6zOkDPxE1? NWL1cHh7OTh|MUO4NFc>
MDA-MB-231 NEjHSolEgXSxdH;4bYMhSXO|YYm= MYW5OkBp MYfEUXNQ NHTpTGlKSzVyPUO2MlYyKM7:TR?= NVHB[GtbOTh|MUO4NFc>
MOLT4 MWTDfZRwfG:6aXOgRZN{[Xl? M33NXVk3KGh? M4LsNGROW09? NELCWIVKSzVyPUG1MlAzKM7:TR?= MWmxPFMyOzhyNx?=
MCF7 MorKR5l1d3SxeHnjJGF{e2G7 M3PreVUxKM7:TR?= MWG3NkBp NHzQZotKSzVyPUGyMlA2KM7:TR?= M{KxN|E5PzdzOEG5
BT474 M3fFW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmizO|IhcA>? M3n4bWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS0O|Qh[2WubIOgc5ZmemW6cILld5NqdmdiRWLCZlIhf2m2aDDFR|UxKG:oIECuN{DPxE1? MorlNVkxOjh2MkS=
HN5 MoL2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXKzdZd1PzJiaB?= NYL1bWNRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUlUh[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkB4cXSqIFXDOVAhd2ZiMD6wNUDPxE1? Mnn1NVkxOjh2MkS=
BA/F3 NXTEUoI2S3m2b4TvfIlkKEG|c3H5 MknaO|IhcA>? MlG3R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCHR1\SJGw5PTiUIH31eIFvfCC5aYToJGlEPTBib3[gNE4xOTB6IN88US=> NHXacJAyQTJ|OUKyPS=>
NIH3T3 M4\0Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\McXhSPzJiaB?= Ml3PS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> Mn\ZNVk3PjV|N{e=
MCF7 MX NVfEelBwTnWwY4Tpc44hSXO|YYm= M2nwSVMxKG2rbh?= M1HVXmlvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhf2m2aDDJR|UxKG:oIEGuNUDPxE1? M320Z|E6QTN{OU[w
A431 NYDx[IZET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfkOWIyOCEQvF2= MYG3NkBp MmHLS2k2OD1yLkWxOEDPxE1? NV;tOmJROTl7Nkm0OlU>
A431 MWLLbY5ie2ViQYPzZZk> M4DWWVAvOSEQvF2= MY[xJIg> M1nzUGlvcGmkaYTpc44hd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> MV[xPVk3QTR4NR?=
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NCI-H1975 NV\zcI8xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXGXZA2KM7:TR?= MYe3NkBp M3SxRmlEPTB;OD6yOkDPxE1? M{DN[VIxOTVzNkew
NCI-H1975 NELoPZNMcW6jc3WgRZN{[Xl? M{TJ[|ExKM7:TR?= M2T5cGlvcGmkaYTpc44hd2ZiRVfGVkBifXSxcHjvd5Bpd3K7bHH0bY9v M1e2elIxOTVzNkew
NCI-H1975 NV\PXotOTnWwY4Tpc44hSXO|YYm= NWXNPHJ6OTBizszN M3\TeGlvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NVzuZWFjOjBzNUG2O|A>
NCI-H1975 NIniV5ZHfW6ldHnvckBCe3OjeR?= NEnZUIQ2KM7:TR?= MUC0PEBp MXLJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4Yh[2G|cHHz[U0{ NWDidItPOjBzNUG2O|A>
A375 MoS2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\zTG5EPDhiaB?= M{i3VGlEPTB;MkCuOVUh|ryP NGPzeFYzODNyNEWzOy=>
SMMC7721 M2DEdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3ITpRQPDhiaB?= MlzMTWM2OD1|Mz6xNkDPxE1? NGWwPG8zODNyNEWzOy=>
DU145 M{PBXGN6fG:2b4jpZ{BCe3OjeR?= NWfVcnJDPSCm NF7INHpFVVOR MlnCTWM2OD12Mj6xO{DPxE1? M4fBb|IxPDZ4NUW1
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PANC1 MU\DfZRwfG:6aXOgRZN{[Xl? NVXMNFFqPSCm NGjKfYdFVVOR NH7xWZZKSzVyPUSwMlQzKM7:TR?= M4D5XVIxPDZ4NUW1
PC9 M2e1Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFe2RVlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN MV[yNVIxQDhyMh?=
PC9 NVrWPFliT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXmSpBjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBoem:5dHitdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDlfJBz\XO|aX7nJGVITlJiRUe0On9CPzVyL2S3PVBOKG23dHHueEB4cXSqIFXDOVAhd2ZiNzFOwG0> MlLjNlEzODh6MEK=
PC3 MknyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTUNVAh|ryP MlrxO|IhcA>? NF63OZBFVVOR NI\0d5hKSzVyPUeuOEDPxE1? NIW2TXEzOTN3M{W0Oi=>
MDA-MB-468 M3njVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\Gc4oyODBizszN MmjZOFghcA>? NHfM[oxFVVOR NUPJdmZOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> M{\xc|IyPDJ7NkOy
Plasmodium falciparum M1HxOZFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w MWSyNVgyPzB2NR?=
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NCI-H292 MoDSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfhO|IhcA>? NY\Eb|NGTE2VTx?= MmHQTWM2OD1yLkCyJO69VQ>? M3X6d|IyQTF4NEOz
A431 NXfZc2JlU2mwYYPlJGF{e2G7 M2nyd|IhcA>? NIXTOIJFVVOR Mnj2TY5pcWKrdHnvckBw\iCHR1\SJJBpd3OyaH;yfYxifGmxbjDpckBGT0Zvc4TpcZVt[XSnZDDoeY1idiCDNEOxJJdqfGhiSVO1NEBw\iByLkCwNUDPxE1? Ml3MNlIyODFzM{K=
MCF7 NY\nR5RWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPsSJIyODEEoN88[{9uVA>? NVHvVJRFPDhiaB?= NUjTT3dTTE2VTx?= MnrwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN NVTLeXFjOjJzMUmxN|A>
BT474 NESzc3BEgXSxdH;4bYMhSXO|YYm= NY\CS|RwOTBizszN NUDjRpZIPDhiaB?= NF7BcYhEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIFLUOFc1KGOnbHzz M4niN|IzOTN6M{C4
MCF7 M{PDRWN6fG:2b4jpZ{BCe3OjeR?= MonJNVAh|ryP M1OxWFQ5KGh? M{XWZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iTVPGO{Bk\WyuczDveoVzNWW6cILld5NqdmdiRYLiRlIh[2WubIO= MmnCNlIyOzh|MEi=
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HCC827 NIPV[2hMcW6jc3WgRZN{[Xl? NYPD[XROOC5zMkWg{txO M3rIXFI1KGh? MVjEUXNQ Ml7wTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEGtdDDwbI9{eGixconsZZRqd25? NHXQelkzOjN|OUO0Ni=>
A431 MUjLbY5ie2ViQYPzZZk> NEm3fIUyODBizszN MojnN|AhdWmw NGX3bI9KdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN MoOyNlI3QTh5OEK=
H460 M{PxbmN6fG:2b4jpZ{BCe3OjeR?= NInyZmQ4OiCq M3zMeGlEPTB;NT61PUDPxE1? NWfrNWZxOjJ5Mke0OFk>
HT-29 NGT3OZBEgXSxdH;4bYMhSXO|YYm= NXv6VHR5PzJiaB?= Mnj3TWM2OD1|LkO2JO69VQ>? NVPrbZJCOjJ5Mke0OFk>
SGC7901 M2LqeGN6fG:2b4jpZ{BCe3OjeR?= NUjzSVFMPzJiaB?= NWTUV2JMUUN3ME2xNE4zPiEQvF2= M{TQXFIzPzJ5NES5
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Sf9 M163b2tqdmG|ZTDBd5NigQ>? NIHMN|UyKGh? M4DwRmROW09? M2[yeGlvcGmkaYTpc44hd2ZiaIXtZY4hT1OWLYTh[4dm\CCHR1\SJGw5OzSUIH31eIFvfCCneIDy[ZN{\WRid3n0bEBKSzVyIH;mJFAvODF3IN88US=> NXvaWmVzOjN4MUG2PVE>
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16HBE NWjWUHY3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHf5ZmU4OiCq NIjPboxFVVOR NITBUY1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIEG2TGJGKGOnbHzzJIV5eHKnc4Ppcochf2muZDD0fZBmKEWJRmKge4l1cCCLQ{WwJI9nKDFwNkCxJO69VQ>? MV:yN|c6OjNzOB?=
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A549 NVfVTlZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEX6NYc4OiCq MlLCSG1UVw>? MmL1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgSWdHWi:tLWLhd{Bl\XCnbnTlcpQhcHWvYX6gRVU1QSClZXzsd{B4cXSqIFnDOVAhd2ZiND62NlYh|ryP M4GzTlI{Pzl{M{G4
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A549 NF7NPWFMcW6jc3WgRZN{[Xl? NUfmfYwxOSEQvF2= NHH0U48yKGh? NVvtZ4NFTE2VTx?= NEXURolKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzl7IN88US=> MlXiNlM6QDh|NUS=
A549 MW\GeY5kfGmxbjDBd5NigQ>? M3;jVlEh|ryP NXv4Z2lrOSCq MVLEUXNQ NX3YN5FyS2WubIXsZZIhfXC2YXvl NYnZ[ol3OjN7OEizOVQ>
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SCC-15 MmLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\GTWM2OD13MkWuOlghdk1? M3W0OXNCVkeHUh?=
NCI-H358 Mo\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITTNZpKSzVyPUWyO{43PiCwTR?= M3;sWXNCVkeHUh?=
KYSE-180 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmD2TWM2OD13M{CuOlIhdk1? MVjTRW5ITVJ?
NCI-H82 MmHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWS4RmpCUUN3ME21OFAvPSCwTR?= M1rEbXNCVkeHUh?=
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CAN M1Kz[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1[0d2lEPTB;NUiwMlc3KG6P M2fIe3NCVkeHUh?=
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NCI-H1623 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTV7OT6zOkBvVQ>? MmrTV2FPT0WU
FADU M33ZOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoS0TWM2OD14MESuOlkhdk1? M{POdXNCVkeHUh?=
NB69 MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\CcI5lUUN3ME22NVQvOzVibl2= MYfTRW5ITVJ?
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LB1047-RCC MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjldo5lUUN3ME22NlUvOjlibl2= NFLDXZlUSU6JRWK=
MV-4-11 NX[xV4dCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HodWlEPTB;NkK3Mlchdk1? MYLTRW5ITVJ?
DBTRG-05MG NFLne4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTZ|Mj6yPEBvVQ>? MVrTRW5ITVJ?
ME-180 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTZ|Mj65JI5O M37OZ3NCVkeHUh?=
NBsusSR MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlznTWM2OD14NE[uOVIhdk1? M{XFfnNCVkeHUh?=
COR-L105 M2fT[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33iWWlEPTB;Nk[yMlU{KG6P MnWxV2FPT0WU
ETK-1 MnTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rWRmlEPTB;Nk[yMlU6KG6P NG\ibGVUSU6JRWK=
L-363 MmHiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\B[4N4UUN3ME22O|EvQTRibl2= MYrTRW5ITVJ?
LU-134-A NXfjbZB4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnSxTWM2OD15MUmuNFkhdk1? MXnTRW5ITVJ?
NCI-H1770 M1KyfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTzV201UUN3ME23NlIvPCCwTR?= NET1ZVRUSU6JRWK=
HAL-01 NFm4SINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTd|ND63NUBvVQ>? MVHTRW5ITVJ?
COLO-684 MnizS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\hfWlEPTB;N{WzMlAyKG6P MmDhV2FPT0WU
TE-6 MnXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTd4ND6zNUBvVQ>? Mlr5V2FPT0WU
A431 NWTYT5JPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLCXFlpUUN3ME23PFAvODRibl2= MnP6V2FPT0WU
LU-135 MnPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\XPI1KSzVyPUe4N{4xOyCwTR?= M2jwS3NCVkeHUh?=
EC-GI-10 Mn21S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1n2eWlEPTB;N{i4MlUzKG6P MlPHV2FPT0WU
TE-12 NIDzWWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHy4d5NKSzVyPUiyNU4yOSCwTR?= MkXIV2FPT0WU
LoVo MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3joUWlEPTB;OEW5MlA3KG6P Mli1V2FPT0WU
23132-87 MoraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDZO3NWUUN3ME24OlQvOjhibl2= NYrCVnpxW0GQR1XS
AU565 NHz5cWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLNd|ZKSzVyPUi2PU43KG6P M4fTd3NCVkeHUh?=
DEL M1jrZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWOwfZVuUUN3ME24O|cvPjVibl2= MnzOV2FPT0WU
COR-L88 M4DpRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTh6Mj6yOkBvVQ>? MkDmV2FPT0WU
NCI-H2126 NHz0NplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFOyTJZKSzVyPUi4Nk45OyCwTR?= M1vDb3NCVkeHUh?=
CAPAN-1 NXzPe4tvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTnTWM2OD16OEOuNlkhdk1? NWDONVZtW0GQR1XS
BT-474 MnTIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrRTWM2OD17M{iuNVQhdk1? Mof4V2FPT0WU
BHY M2XX[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjjOldlUUN3ME25O|UvPDhibl2= MVXTRW5ITVJ?
HT-3 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TIbWlEPTB;OUiyMlQyKG6P MoPXV2FPT0WU
SK-NEP-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XqSGlEPTB;OUi5MlQ1KG6P MlTQV2FPT0WU
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NCI-H1304 NYCzXW8xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV25cpM6UUN3ME2xMlAyPjR|IN88US=> MXzTRW5ITVJ?
EM-2 NVf3UG9{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYG4PXU5UUN3ME2xMlA{ODF4IN88US=> MXrTRW5ITVJ?
MHH-PREB-1 NI[we2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVSx[5BRUUN3ME2xMlA5PDVzIN88US=> M1e2W3NCVkeHUh?=
KU-19-19 M2LoTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTFwMEi1OFEh|ryP NUjNcmdHW0GQR1XS
NCI-H1648 NGjUV|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTFwMUC2NVYh|ryP NVjHTW9uW0GQR1XS
MKN1 NUXTOXJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrCTWM2OD1zLkKxPVg4KM7:TR?= M3fuT3NCVkeHUh?=
SNU-449 NHSxVXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\uTWM2OD1zLkKyNlA5KM7:TR?= NVjQdVJzW0GQR1XS
OAW-42 NGXEVVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPucotKSzVyPUGuNlc5OjVizszN NXHwd3dpW0GQR1XS
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SW1088 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTFwMkm2NFch|ryP M4\5THNCVkeHUh?=
GCIY NIT6PYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTFwM{CxN|Ih|ryP NF;wPFJUSU6JRWK=
CTV-1 Moj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mom1TWM2OD1zLkOwPEDPxE1? NELvPGNUSU6JRWK=
NCI-H1092 NVvPcnlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\tTWM2OD1zLkOwPFUh|ryP NX\iT5I6W0GQR1XS
NKM-1 M{jJPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPpc3lQUUN3ME2xMlMzPTl5IN88US=> MXLTRW5ITVJ?
NCI-H1650 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LCSmlEPTB;MT6zN|Q5OiEQvF2= MXrTRW5ITVJ?
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KMOE-2 M131W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGOyVmNKSzVyPUGuN|k5OTVizszN MXjTRW5ITVJ?
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RH-1 M1fpSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULuUXVDUUN3ME2xMlQyOTN4IN88US=> MYnTRW5ITVJ?
NCI-H526 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzoXFRKSzVyPUGuOFM5OjZizszN Ml63V2FPT0WU
697 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrLSmlKSzVyPUGuOFQyODRizszN Mo\4V2FPT0WU
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BE-13 MonsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\YTWM2OD1zLkWwOlcyKM7:TR?= M2rTW3NCVkeHUh?=
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SCC-25 M1zodWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\KTWM2OD1zLkWyN|IzKM7:TR?= MlTpV2FPT0WU
LAMA-84 M3jOe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTFwNUO4NVUh|ryP NGKwbmVUSU6JRWK=
HSC-3 NELaZ|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorhTWM2OD1zLkW0N|gh|ryP NGr2[GRUSU6JRWK=
BT-549 NF3h[GpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofxTWM2OD1zLkW4O|Q4KM7:TR?= M{X4dnNCVkeHUh?=
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BB30-HNC M4raZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTFwNki5O|Ih|ryP M{DTXXNCVkeHUh?=
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COLO-678 M{HMdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrDOo9sUUN3ME2xMlc{PDR2IN88US=> MWfTRW5ITVJ?
NCI-H1666 NVjmd49CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4L3d2lEPTB;MT63OFcxPCEQvF2= MXrTRW5ITVJ?
NCI-H209 NIHQc25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnSUpRTUUN3ME2xMlc4OjZ7IN88US=> NF2yb5hUSU6JRWK=
EPLC-272H NX6zeWRHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\uTHdLUUN3ME2xMlg2PTR7IN88US=> MYfTRW5ITVJ?
OMC-1 NVfnOIpWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3e5XWlEPTB;MT65PVg5QCEQvF2= Mn73V2FPT0WU
HCC70 NFTwWmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHCSGRKSzVyPUKuNFA5OTVizszN MlL3V2FPT0WU
CAL-12T NUD5VYxsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDNTWM2OD1{LkCxPFYyKM7:TR?= NWL1[IozW0GQR1XS
TGBC24TKB M4nPNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnEN4V{UUN3ME2yMlAzQTZzIN88US=> NYXzRngzW0GQR1XS
EKVX MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnjd2x5UUN3ME2yMlA1QTN7IN88US=> MXTTRW5ITVJ?
SW1116 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTjTWM2OD1{LkC2NFA6KM7:TR?= MkfIV2FPT0WU
NCI-H2009 M16wR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTJwME[yNVQh|ryP MoPWV2FPT0WU
RPMI-8866 MnP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LFU2lEPTB;Mj6wPFQ5OSEQvF2= NIXVc3VUSU6JRWK=
KARPAS-45 M3;V[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TjZ2lEPTB;Mj6xNlQ4QSEQvF2= MmPXV2FPT0WU
RXF393 MnHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoixTWM2OD1{LkGzNFM3KM7:TR?= M13ncHNCVkeHUh?=
786-0 M1njRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTJeYd1UUN3ME2yMlE5PDB4IN88US=> NF[5c4FUSU6JRWK=
SCC-4 MkjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTJwMkG3O|Yh|ryP NVzFclAxW0GQR1XS
BT-20 NFvXbWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTJwMk[3OFQh|ryP NVf6fXg3W0GQR1XS
A498 MljFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLVTWM2OD1{LkK5OFE2KM7:TR?= NVztfoh{W0GQR1XS
IGROV-1 NX7jUnpoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVX3cY9XUUN3ME2yMlMyOjN5IN88US=> NX;0XXNrW0GQR1XS
JVM-3 NYn0U4VsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPOeIlKSzVyPUKuN|Q3PDNizszN MoD1V2FPT0WU
NCI-H2170 MoLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTJwM{S3PFMh|ryP MXPTRW5ITVJ?
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KU812 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTtZYtVUUN3ME2yMlM3Pzd7IN88US=> NGG1[IdUSU6JRWK=
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HH MoXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LIXGlEPTB;Mj60PVg2PCEQvF2= M13IUHNCVkeHUh?=
MKN7 MkjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPUSo1KSzVyPUKuOlI4PDhizszN NVrQRnVpW0GQR1XS
TE-8 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\PfmlEPTB;Mj62N|Y2OyEQvF2= NW[wcWhDW0GQR1XS
MC-IXC M{DvUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\nTWM2OD1{Lk[0OFU{KM7:TR?= NYj0e3FmW0GQR1XS
DMS-79 NITobVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTJwNkmyPFQh|ryP NUXUN2VMW0GQR1XS
Detroit562 MmDaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkD1TWM2OD1{Lk[5PFQ2KM7:TR?= NHnGNVBUSU6JRWK=
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QIMR-WIL NIDOR4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLlUXExUUN3ME2yMlc2QTV{IN88US=> MULTRW5ITVJ?
BB65-RCC NV\qd21ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGntemRKSzVyPUKuPFA4OTNizszN M2C5e3NCVkeHUh?=
PC-3 Ml7wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTJwOEOzNVch|ryP NXHFcmJHW0GQR1XS
OVCAR-3 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUX3UWNbUUN3ME2yMlg2PTl2IN88US=> Mo\hV2FPT0WU
KYSE-510 MlvSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTJwOE[3PFYh|ryP MmTWV2FPT0WU
HC-1 MnL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LSfWlEPTB;Mj65OFAyOiEQvF2= NGeyS3BUSU6JRWK=
NCI-H1693 NWfURY9CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M373emlEPTB;Mj65OVg6PyEQvF2= M4\5cXNCVkeHUh?=
DU-4475 Mn7nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXVTWM2OD1{Lkm5NFUzKM7:TR?= MYLTRW5ITVJ?
COLO-680N M3LBZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFGzRZlKSzVyPUOuNFA1QTVizszN NWTuXZo6W0GQR1XS
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BCPAP NH3yRmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTjTWM2OD1|LkC4Nlk2KM7:TR?= NFXqT5ZUSU6JRWK=
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NCI-H1838 NV;IOm5ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjQ[I5KSzVyPUWuNFIxPzZizszN NV\WRmh{W0GQR1XS
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BFTC-905 MlrqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7TVIZKSzVyPUWuOlU5PzhizszN MoqwV2FPT0WU
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HCC1187 M3\3Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXO3SHpUUUN3ME21MlcyODN{IN88US=> M{i0NHNCVkeHUh?=
HCC2998 NEL0WZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnToTWM2OD13LkexPFc4KM7:TR?= NF7vd5RUSU6JRWK=
RT-112 MojuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTVwOUCyOFUh|ryP NUDiNlNjW0GQR1XS
CAKI-1 NETFb|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zCTWlEPTB;Nj6wNFk5PSEQvF2= NFrHN4FUSU6JRWK=
HDLM-2 NGD2bohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnwW4xKSzVyPU[uNFE1QDlizszN MUDTRW5ITVJ?
TE-10 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;UXIZoUUN3ME22MlA1QDN|IN88US=> NWTxPXNZW0GQR1XS
KE-37 NWHZfIZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvUTWM2OD14LkC3NlU5KM7:TR?= MXTTRW5ITVJ?
RCM-1 NV3K[4l7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTZwMEe3NVkh|ryP M{frNnNCVkeHUh?=
Mewo MmXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTZwMUG0PFch|ryP NVrBTo1QW0GQR1XS
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8-MG-BA NVz1V3c6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTZwMUmzO|kh|ryP MV;TRW5ITVJ?
SK-MEL-28 M1vKNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLvfZBOUUN3ME22MlIxQTZ5IN88US=> NVX2TpNGW0GQR1XS
VA-ES-BJ MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnWNmh3UUN3ME22MlMxPDZ{IN88US=> NI\1OZNUSU6JRWK=
CAL-120 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofyTWM2OD14LkS1NVYzKM7:TR?= MVXTRW5ITVJ?
GAMG MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;peG1KSzVyPU[uOVA3OiEQvF2= NVn0S2p[W0GQR1XS
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SH-4 M2T6[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVL1SoNPUUN3ME22MlU5QDR2IN88US=> MlnIV2FPT0WU
Calu-3 NVLEbG5VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrOTWM2OD14Lk[1OVY5KM7:TR?= NV3TWYN1W0GQR1XS
SK-HEP-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTqTWM2OD14LkexO|U{KM7:TR?= MlvNV2FPT0WU
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A388 NWj0SJlUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTZwOEm1NkDPxE1? NGTmOGxUSU6JRWK=
KLE MlXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LGT2lEPTB;Nj65OlUyPiEQvF2= MWrTRW5ITVJ?
TE-11 NHq1[pFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TGNGlEPTB;Nz6wOFk3PyEQvF2= MVjTRW5ITVJ?
NCI-H2405 NYf6emtjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXztPHZHUUN3ME23MlA5Ozl|IN88US=> MWDTRW5ITVJ?
OVCAR-4 NF;IOpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrjWmpKSzVyPUeuNVU{PSEQvF2= M3HJbXNCVkeHUh?=
KP-N-YS NF;kUYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTdwMUixOEDPxE1? M3HqTXNCVkeHUh?=
NOS-1 MknhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XYXmlEPTB;Nz6yOVgzQCEQvF2= MWXTRW5ITVJ?
DMS-114 NVLofWZET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTdwMkm2N|Eh|ryP M4ja[nNCVkeHUh?=
NCI-H2030 MlLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDiTWM2OD15LkOzOlk5KM7:TR?= M{TsR3NCVkeHUh?=
HEL NUXuUnNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHHTWM2OD15LkO3OVIyKM7:TR?= MVTTRW5ITVJ?
CAL-62 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkiyTWM2OD15LkO5PFY3KM7:TR?= M1TEXXNCVkeHUh?=
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SKG-IIIa MkOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3wOW1TUUN3ME23MlQ2OzhzIN88US=> MlTwV2FPT0WU
HCC1419 MonWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTdwNE[4N|Mh|ryP NFX3eoFUSU6JRWK=
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COLO-829 NFPJPIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTdwNk[0OlMh|ryP NV;ESI9HW0GQR1XS
NB13 M3LGUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTdwOEK2Olch|ryP NID4eW9USU6JRWK=
DK-MG NXHzeXNjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3THTmlEPTB;Nz64N|IxOSEQvF2= NW\Ub5hlW0GQR1XS
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NCI-H661 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRThwMEW4Olch|ryP M3O2SXNCVkeHUh?=
AM-38 Mnj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLrTWM2OD16LkG2Nlgh|ryP NYe2SGVZW0GQR1XS
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D-392MG MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXqeHlKSzVyPUiuOlYzOTRizszN NETEU4lUSU6JRWK=
A673 NWPxbmxoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRThwNke2NVEh|ryP M1PzeHNCVkeHUh?=
COLO-792 NYq3OpAzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHmTWM2OD16Lke0PVQ3KM7:TR?= NUO2U2cyW0GQR1XS
NCI-H1792 M3vaTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX61WVd5UUN3ME24Mlg5QTB|IN88US=> NUDmOnp5W0GQR1XS
LS-1034 M1H1WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTFTnlrUUN3ME24Mlk1PjV{IN88US=> NFTGfWxUSU6JRWK=
D-566MG NYHNS2NET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTlwMEG4NUDPxE1? M4LaRXNCVkeHUh?=
NCI-H226 M{m4emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HyUWlEPTB;OT6wPUDPxE1? NH\TWIZUSU6JRWK=
ChaGo-K-1 NGfXTo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH:5ZmtKSzVyPUmuNVA2PzlizszN MV7TRW5ITVJ?
NCI-H2228 NVfo[VRlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmewTWM2OD17LkGxNFgyKM7:TR?= NED0NmZUSU6JRWK=
RPMI-8226 NVuzXldST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1f2ZmlEPTB;OT6xNVMxPCEQvF2= NIPMXoRUSU6JRWK=
BFTC-909 NILrZmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HjZ2lEPTB;OT6xPFI6PyEQvF2= NVfheWVqW0GQR1XS
HPAF-II NYe3R5VnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUP1O2oyUUN3ME25MlIzPTF6IN88US=> NFHHW4hUSU6JRWK=
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H-EMC-SS NED3[IdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fCW2lEPTB;NEWuO|Y6OyEQvF2= MlnNV2FPT0WU
NCI-H2052 M2[1SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3n0VmlEPTB;NEWuPVAzPyEQvF2= NHe4SHVUSU6JRWK=
SW684 NHTafYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlj6TWM2OD12Nz6wNFgyKM7:TR?= MXrTRW5ITVJ?
HCC1569 NHnKb2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4CyWWlEPTB;NEeuNVY2QCEQvF2= NE[2[ndUSU6JRWK=
KGN MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\UWWtDUUN3ME20O{4{OzFzIN88US=> M2DOcHNCVkeHUh?=
LCLC-103H NFPaSllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jQ[2lEPTB;NEeuOFQxPCEQvF2= M4HRTnNCVkeHUh?=
SW780 M4D2SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvVR25KSzVyPUS3MlY{PTFizszN NUTtbVNSW0GQR1XS
NCI-H1703 M2e4bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTnbJIyUUN3ME20PE4{OzV{IN88US=> MVXTRW5ITVJ?
HCC1395 NITsWGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHu[WpKSzVyPUS4Mlc1OzJizszN MXzTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
phospho-EGFR / phospho-ERK / phospho-SRC / phospho-AKT; 

PubMed: 22586683     


Effect of overexpressing CRKL on phosphorylation levels of EGFR, ERK, AKT and SRC in response to gefitinib. HCC827 cells expressing a control vector or CRKL were exposed to gefitinib at indicated concentrations for 6 h and cell lysates were collected for immunoblotting with indicated specific antibodies.

Cyclin D1/ p16 / p27 / pRb; 

PubMed: 18559346     


levels of phosphorylated and total EGFR, IGF-1R, Akt, ERK1/2, and Rb and levels of cyclin D1, p27, p16, and actin following 6- or 16-h treatment of A431 cells with combinations of 1 or 3 μm gefitinib and 3 μm NVP-ADW742. Data are representative of two independent experiments.

uc-caspase-9 / c-caspase-9 / uc-caspase-3 / c-caspase-3 / uc-PARP / PARP; 

PubMed: 23102728     


Apoptosis resulting from combined treatment with gefitinib and an MEK inhibitor. (A) apoptosis relating protein induced by gefitinib and/or AZD6244 in PC‐9/wt and PC‐9/gef B4 cells. (B) Apoptosis relating protein induced by gefitinib and/or CI‐1040 in PC‐9/wt and PC‐9/gef B4 cells. (C) apoptosis relating protein induced by gefitinib and/or AZD6244 in PC‐9/gefE3 and PC‐9/gefE7 cells. (D) apoptosis relating protein induced by gefitinib and/or CI‐1040 in PC‐9/gefE3 and PC‐9/gefE7 cells.

PPAR-γ; 

PubMed: 23409107     


Fold increase relative to control was determined after normalization with β-actin as external loading control. 

22586683 18559346 23102728 23409107
Immunofluorescence
EGFR / LC3; 

PubMed: 31196210     


H157 and H1299 cells were treated with gefitinib, or curcumin alone, or gefitinib plus curcumin at indicated concentration for 48 h and then were fixed with methanol, immunostained with anti-EGFR (green), anti-LC3 (red), and DAPI (blue), and analyzed by confocal microscopy to determine the intracellular locations of EGFR and LC3.

PPAR-γ; 

PubMed: 23409107     


Upregulation and nuclear accumulation of PPARγ following treatment with gefitinib (24 hs).

p-EGFR / β-catenin; 

PubMed: 23461856     


In untreated CNE2 cells, β-catenin was located in cytoplasmic membranes and cytoplasm. Treatment with EGF (50 ng/mL) resulted in increased β-catenin nuclear staining, which was reversed by either gefitinib (1 μM) or LY294002 (15 μM). 

31196210 23409107 23461856
Growth inhibition assay
GI50; 

PubMed: 23409107     


Dose-response of bladder cancer cell lines to EGFR inhibitor (gefitinib). The GI50 value was defined as the mean concentration of drug that generates 50% of growth inhibition as compared to controls.

23409107
In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
- Collapse
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
- Collapse
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms Iressa
Smiles COC1=C(C=C2C(=C1)N=CN=C2NC3=CC(=C(C=C3)F)Cl)OCCCN4CCOCC4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03292133 Recruiting Drug: EGF816|Drug: Gefitinib Lung Cancer Massachusetts General Hospital|Novartis October 31 2017 Phase 2
NCT03122717 Recruiting Drug: Gefitinib|Drug: Osimertinib Non-Small Cell Lung Cancer Dana-Farber Cancer Institute|AstraZeneca May 9 2017 Phase 1|Phase 2
NCT02932345 Completed -- EGFR Mutation Positive Advanced Non-small-cell Lung Cancer AstraZeneca October 31 2016 --
NCT02824458 Recruiting Drug: Apatinib|Drug: Gefitinib|Drug: Placebo EGFR Tyrosine Kinase Inhibitors Plus VEGFR Inhibitors Sun Yat-sen University June 2016 Phase 3
NCT02804100 Unknown status -- Carcinoma Non-Small-Cell Lung|Bronchial Neoplasms|Carcinoma Bronchogenic|Lung Diseases|Lung Neoplasms Wenzhou Medical University May 2016 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID