Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 95 Publications

14 Customer Reviews

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MoraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTBwMEW0JO69VQ>? MYqxOlUyPjR5Mx?=
NIH3T3 MnK2SpVv[3Srb36gRZN{[Xl? MWGwMlIh|ryP NEDaTHEzKGh? MmPUSG1UVw>? NELvVWlKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? MoLQNVY1QDB{OES=
COS7 M4HpR2Z2dmO2aX;uJGF{e2G7 M2rkSlk3KM7:TR?= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGpCUzJiVk[xO2YhdXW2YX70JIV5eHKnc4Pl[C=> MW[xO|E4QDd{Mh?=
A549 NHrINYlEgXSxdH;4bYMhSXO|YYm= MkTyPVYhcA>? Ml3YSG1UVw>? NH\pco1KSzVyPUGzMlU6KM7:TR?= NIL6SWYyQDNzM{iwOy=>
K562 MkjZR5l1d3SxeHnjJGF{e2G7 MmnGPVYhcA>? Mnu0SG1UVw>? MVPJR|UxRTlwM{[g{txO MkPhNVg{OTN6MEe=
MDA-MB-231 MkL6R5l1d3SxeHnjJGF{e2G7 NHHmfIU6PiCq NYH4OHJWTE2VTx?= MnGyTWM2OD1|Nj62NUDPxE1? NHjPeHAyQDNzM{iwOy=>
MOLT4 NYfiPVh7S3m2b4TvfIlkKEG|c3H5 NH3OdGU6PiCq MW\EUXNQ NUPZXJREUUN3ME2xOU4xOiEQvF2= MVmxPFMyOzhyNx?=
MCF7 NYrmbWpXS3m2b4TvfIlkKEG|c3H5 M4LSXlUxKM7:TR?= M2\FfVczKGh? NFzJOHFKSzVyPUGyMlA2KM7:TR?= MkPjNVg4PzF6MUm=
BT474 Mly5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;HO|IhcA>? MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWNEe0JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWUQnKyJJdqfGhiRVO1NEBw\iByLkOg{txO NIXwe48yQTB{OESyOC=>
HN5 NWTJZlQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvYS4I4OiCq Mk\jRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTkWgZ4VtdHNib4\ldoV5eHKnc4PpcochTUeIUjD3bZRpKEWFNUCgc4YhOC5yMTFOwG0> NHPIWWcyQTB{OESyOC=>
BA/F3 NH7QTWJEgXSxdH;4bYMhSXO|YYm= MnnHO|IhcA>? NYG0VGVWS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSkFxRkOgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEx6NUjSJI12fGGwdDD3bZRpKEmFNUCgc4YhOC5yMUC4JO69VQ>? NYfrcnRtOTl{M{myNlk>
NIH3T3 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHITmRQPzJiaB?= M3G3[mdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJG5KUC9|VEOgZ4VtdHNiZYjwdoV{e2mwZzDldmIzKGenbnWge4l1cCCLQ{WwJI9nKDBwNUSg{txO M3jiZVE6PjZ3M{e3
MCF7 MX MXHGeY5kfGmxbjDBd5NigQ>? M1rGWlMxKG2rbh?= Mnr0TY5pcWKrdHnvckBw\iCEQ2LQJIV5eHKnc4Pl[EB4cXSqIFnDOVAhd2ZiMT6xJO69VQ>? NUHhT4tMOTl7M{K5OlA>
A431 M17sWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTBcJp{OTBizszN M17qSVczKGh? MlrlS2k2OD1yLkWxOEDPxE1? NHX2[ZUyQTl4OUS2OS=>
A431 MWPLbY5ie2ViQYPzZZk> MnXlNE4yKM7:TR?= MW[xJIg> MVzJcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u NW\BfZBtOTl7Nkm0OlU>
A431 M2j0e2tqdmG|ZTDBd5NigQ>? M2PaXVExOCEQvF2= M3LBbFExKG2rbh?= NFm0[Y9Rem:ub37n[YQhcW6qaXLpeI9zgSCjY4Tpeol1gSCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? MmnhNVk6Pjl2NkW=
Sf9 MW\LbY5ie2ViQYPzZZk> M1G5ZVExKG2rbh?= NVvFUYI3TE2VTx?= M{XRS2lvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= M1jJSVIxODV4NEK1
NCI-H1975 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkf6OUDPxE1? NIPUbJU4OiCq NV3wSHk3UUN3ME24MlI3KM7:TR?= NH[5TI8zODF3MU[3NC=>
NCI-H1975 MmKyT4lv[XOnIFHzd4F6 NX;yfXY1OTBizszN NVLKNWxFUW6qaXLpeIlwdiCxZjDFS2ZTKGG3dH;wbI9{eGixconsZZRqd25? MlLUNlAyPTF4N{C=
NCI-H1975 NUG2epFDTnWwY4Tpc44hSXO|YYm= Ml3aNVAh|ryP M2Tr[mlvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? MYGyNFE2OTZ5MB?=
NCI-H1975 NV21c4FKTnWwY4Tpc44hSXO|YYm= NWLVSnBuPSEQvF2= M3\ydVQ5KGh? MnL5TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UuOw>? Ml\INlAyPTF4N{C=
A375 NYX1WGFHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fzSVQ5KGh? NX;BToxHUUN3ME2yNE42PSEQvF2= MWCyNFMxPDV|Nx?=
SMMC7721 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nKR|Q5KGh? MnHjTWM2OD1|Mz6xNkDPxE1? MVKyNFMxPDV|Nx?=
DU145 NF;IeVBEgXSxdH;4bYMhSXO|YYm= NVjPTFBTPSCm NYLFR2M1TE2VTx?= MXfJR|UxRTR{LkG3JO69VQ>? Mn\SNlA1PjZ3NUW=
MIAPaCa2 NH3VRnNEgXSxdH;4bYMhSXO|YYm= NVLx[W4xPSCm NXLWXIlOTE2VTx?= NILhT4JKSzVyPUWwMlYzKM7:TR?= MXiyNFQ3PjV3NR?=
PANC1 MVLDfZRwfG:6aXOgRZN{[Xl? MYC1JIQ> M1W1RmROW09? MnnPTWM2OD12MD60NkDPxE1? MmTuNlA1PjZ3NUW=
PC9 NIr5[ZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fBO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgSWdHWi2mZX\pZ4lmdnRiaIXtZY4hWEN7IHPlcIx{KHerdHigSWM2OCCxZjCwMlAyPyEQvF2= M1m0SFIyOjB6OECy
PC9 MnH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfaW4ZCSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBoem:5dHitdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDlfJBz\XO|aX7nJGVITlJiRUe0On9CPzVyL2S3PVBOKG23dHHueEB4cXSqIFXDOVAhd2ZiNzFOwG0> MW[yNVIxQDhyMh?=
PC3 NFq4cHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlH0NVAh|ryP MlyyO|IhcA>? MoS0SG1UVw>? NVTifFdEUUN3ME23MlQh|ryP MUiyNVM2OzV2Nh?=
MDA-MB-468 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXmxNFAh|ryP NU\rcGRSPDhiaB?= NWXDWItKTE2VTx?= MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eR?= M3q5U|IyPDJ7NkOy
Plasmodium falciparum Mmf2dWhVWyCycn;mbYxqdmdiZn;yJIlvcGmkaYTvdpMhd2ZiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJJBzd2yrZnXyZZRqd25? NIHaNlkzOThzN{C0OS=>
NCI-H1993 NUP4TWJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nzRVczKGh? MUnEUXNQ NFPGR4RKSzVyPUCuNUDPxE1? MV2yNVkyPjR|Mx?=
NCI-H292 MkjZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV23NkBp MnXHSG1UVw>? NXSw[41JUUN3ME2wMlAzKM7:TR?= NGjEOZozOTlzNkSzNy=>
A431 NUP1cpJHU2mwYYPlJGF{e2G7 NV\XV5V2OiCq NGryW5ZFVVOR Mnu5TY5pcWKrdHnvckBw\iCHR1\SJJBpd3OyaH;yfYxifGmxbjDpckBGT0Zvc4TpcZVt[XSnZDDoeY1idiCDNEOxJJdqfGhiSVO1NEBw\iByLkCwNUDPxE1? NHPSU4QzOjFyMUGzNi=>
MCF7 M4r1Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFr2XGoyODEEoN88[{9uVA>? MWC0PEBp M{Ph[WROW09? NGXm[4VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> NXXnRVJbOjJzMUmxN|A>
BT474 MoLuR5l1d3SxeHnjJGF{e2G7 NGj1OWcyOCEQvF2= MYq0PEBp MkHDR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= NEG1[YozOjF|OEOwPC=>
MCF7 NInWVm9EgXSxdH;4bYMhSXO|YYm= NYD1VnRqOTBizszN NIPYToE1QCCq MXfDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKE2FRkegZ4VtdHNib4\ldk1mgHC{ZYPzbY5oKEW{YlKyJINmdGy| NYjlVJVvOjJzM{izNFg>
HCC827 NUTlNm9EU2mwYYPlJGF{e2G7 NV;KTXdTOC5zMkWg{txO NHPoPVAzPCCq M1\r[2ROW09? Mnr5TY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51KFlzME[4JIF2fG:yaH;zdIhwenmuYYTpc44> MnTYNlI{Ozl|NEK=
HCC827 M3\XWGtqdmG|ZTDBd5NigQ>? M4\CXlAvOTJ3IN88US=> NFvOfpIzPCCq NV30O2NUTE2VTx?= MlTJTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEW{azDwbI9{eGixconsZZRqd25? M2riOFIzOzN7M{Sy
HCC827 Mki2T4lv[XOnIFHzd4F6 NHXXZVAxNjF{NTFOwG0> NWLBVodjOjRiaB?= NGOwNJVFVVOR NWjSNWdnUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= MkjXNlI{Ozl|NEK=
A431 NEGyZ3pMcW6jc3WgRZN{[Xl? M{Pkd|ExOCEQvF2= NX3ZOHdrOzBibXnu MWjJcohq[mm2aX;uJI9nKEWJRmKg[IVzcX[nZDDmdo9uKGi3bXHuJGE1OzFiY3XscEBu\W2kcnHu[UBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJBwdHlvR3z1MXR6eiCyaH;zdIhwenmuYYTpc44hf2m2aDDJR|UxKG:oIEGuNFgh|ryP NVH4OGMxOjJ4OUi3PFI>
H460 NXnKOJIyS3m2b4TvfIlkKEG|c3H5 NYDoZW43PzJiaB?= M{\yUWlEPTB;NT61PUDPxE1? NYmzeFJ7OjJ5Mke0OFk>
HT-29 NWX1eHlsS3m2b4TvfIlkKEG|c3H5 NFnTSlc4OiCq M1;6R2lEPTB;Mz6zOkDPxE1? M3nC[|IzPzJ5NES5
SGC7901 NGG4RXdEgXSxdH;4bYMhSXO|YYm= Mof6O|IhcA>? MVXJR|UxRTFyLkK2JO69VQ>? NX7kcnJGOjJ5Mke0OFk>
A498 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7le5JsSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFk5KGOnbHzzJIV5eHKnc4PpcochTUeIUjDhcoQhW1KFIIfpeIghUUN3MDDv[kA5NjZizszN MWOyNlgyQDh2OB?=
Calu3 NUPBeYUyS3m2b4TvfIlkKEG|c3H5 MYSxNEDPxGdxbVy= NVXwOYNuPzJiaB?= MWnJR|UxRTFwNjFOwG0> M{HqPFI{OTF4MU[4
FADU MkDwR5l1d3SxeHnjJGF{e2G7 M{nhWlExKM7:Zz;tUC=> M1uzZ|czKGh? MVLJR|UxRTBwMEWg{txO NYL1dHpoOjNzMU[xOlg>
HCC827 NWnTUnBnS3m2b4TvfIlkKEG|c3H5 M{DYWlExKM7:Zz;tUC=> Mn7nO|IhcA>? MYHJR|UxRTBwMECxOUDPxE1? MX2yN|EyPjF4OB?=
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Sf9 NULUWIFtU2mwYYPlJGF{e2G7 MWqxJIg> MX7EUXNQ NEjTfolKdmirYnn0bY9vKG:oIHj1cYFvKEeVVD30ZYdo\WRiRVfGVkBNQDN2UjDteZRidnRiZYjwdoV{e2WmIIfpeIghUUN3MDDv[kAxNjBzNTFOwG0> MnS2NlM3OTF4OUG=
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IGROV-1 Ml[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3KyWWlEPTB;Mj6zNVI{PyEQvF2= Mm\EV2FPT0WU
JVM-3 NIWwW5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTETWM2OD1{LkO0OlQ{KM7:TR?= MlLJV2FPT0WU
NCI-H2170 NWqxdWJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\3cWlEPTB;Mj6zOFc5OyEQvF2= MYHTRW5ITVJ?
EW-18 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\DfWpNUUN3ME2yMlM2PDdzIN88US=> M2rQSXNCVkeHUh?=
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NY NWLDUZFnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTJwM{eyO{DPxE1? NUHVdYVOW0GQR1XS
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CFPAC-1 MoXYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfwZ5ZMUUN3ME2yMlQ{QDZ2IN88US=> MXHTRW5ITVJ?
HH NIXyUY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIm5VItKSzVyPUKuOFk5PTRizszN MoT3V2FPT0WU
MKN7 NX7GfmJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DNUWlEPTB;Mj62Nlc1QCEQvF2= NFrSWZRUSU6JRWK=
TE-8 NHLEWZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTJwNkO2OVMh|ryP MljnV2FPT0WU
MC-IXC NWX6WZZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTQTWM2OD1{Lk[0OFU{KM7:TR?= NYrQN3VCW0GQR1XS
DMS-79 NXHvfZE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvOTWM2OD1{Lk[5Nlg1KM7:TR?= MmX6V2FPT0WU
Detroit562 NGXZ[4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTJwNkm4OFUh|ryP MUjTRW5ITVJ?
HuP-T4 Ml\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofSTWM2OD1{LkeyPFE5KM7:TR?= NYLmdYxTW0GQR1XS
QIMR-WIL NHfxU5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTJwN{W5OVIh|ryP NUTjOlJ4W0GQR1XS
BB65-RCC NIP4WGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzaTWM2OD1{LkiwO|E{KM7:TR?= MUjTRW5ITVJ?
PC-3 NVfFZ|V2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDoTWM2OD1{LkizN|E4KM7:TR?= Mk\IV2FPT0WU
OVCAR-3 NGHoUm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTJwOEW1PVQh|ryP MX3TRW5ITVJ?
KYSE-510 NG\sd3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHzTWM2OD1{Lki2O|g3KM7:TR?= NHPTZVBUSU6JRWK=
HC-1 NFvyeXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjCdYdWUUN3ME2yMlk1ODF{IN88US=> Mn21V2FPT0WU
NCI-H1693 NX7KcZUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1n3NGlEPTB;Mj65OVg6PyEQvF2= NUjsdmJVW0GQR1XS
DU-4475 M4fxSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULJdJpuUUN3ME2yMlk6ODV{IN88US=> NILFUXpUSU6JRWK=
COLO-680N NVzTenhyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzYSWJKSzVyPUOuNFA1QTVizszN NFPVbmVUSU6JRWK=
MN-60 NV7pem84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\BeJNZUUN3ME2zMlA1QTd2IN88US=> NGOzeVRUSU6JRWK=
BCPAP MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTNwMEiyPVUh|ryP Mkm5V2FPT0WU
647-V MlrXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTk[2VZUUN3ME2zMlE3OjJizszN MWrTRW5ITVJ?
HT-1376 M2D4cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTNwMkC1NFYh|ryP NI[wXHBUSU6JRWK=
NCI-H1793 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLwR5RzUUN3ME2zMlIyODl6IN88US=> MVrTRW5ITVJ?
SW1463 MkLsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHz4UlZKSzVyPUOuNlYyQTJizszN NWfKXJRQW0GQR1XS
NCI-H727 MmjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknUTWM2OD1|LkO4OVg1KM7:TR?= NVXn[JlCW0GQR1XS
LB771-HNC MoLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVize2I3UUN3ME2zMlM6OTJ4IN88US=> NYSzPYxsW0GQR1XS
D-542MG NGj1dlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrLTWM2OD1|LkS0NlIzKM7:TR?= NILqZZlUSU6JRWK=
NTERA-S-cl-D1 NHnT[5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfuTWM2OD1|LkS0PVU4KM7:TR?= M3G2S3NCVkeHUh?=
C8166 M{L4cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjVXHc{UUN3ME2zMlQ3PTJzIN88US=> MmXvV2FPT0WU
639-V NGS4eXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXDWWdKUUN3ME2zMlU4Pzl{IN88US=> MYDTRW5ITVJ?
TE-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrTeFZtUUN3ME2zMlU6OTZ7IN88US=> MkKzV2FPT0WU
OAW-28 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW2[olxUUN3ME2zMlU6PDJ4IN88US=> MVPTRW5ITVJ?
SK-LU-1 NVTkWlFpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XmO2lEPTB;Mz62NFY1PSEQvF2= M1TCW3NCVkeHUh?=
CaR-1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\ETmFKSzVyPUOuOlExPDhizszN NFjtRYJUSU6JRWK=
A2780 NYjFNI85T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTLNIFKSzVyPUOuOlQxQDlizszN NWLNcnVCW0GQR1XS
C2BBe1 M1;TZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HWXWlEPTB;Mz62OFQxPyEQvF2= NIL6b5VUSU6JRWK=
SK-MES-1 NEDrUFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2PHZWlEPTB;Mz62OFYyPCEQvF2= M3HqNXNCVkeHUh?=
PANC-10-05 M33Jbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjuTWM2OD1|LkeyNFczKM7:TR?= M2LYbXNCVkeHUh?=
J-RT3-T3-5 NHvDd21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTNwN{W0NFYh|ryP NHnUSZpUSU6JRWK=
ES4 Ml7vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXmVFRKSzVyPUOuPFIzQDZizszN MYnTRW5ITVJ?
NCI-H28 Ml[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTZN25KSzVyPUOuPFI6PjFizszN NIf4[3NUSU6JRWK=
NCI-H1155 NYfGXnh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmS5TWM2OD1|Lki0NVU6KM7:TR?= NIn3cGtUSU6JRWK=
KURAMOCHI MnrwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXvTWM2OD1|Lki2NFU3KM7:TR?= NXTiXpA5W0GQR1XS
NCI-H69 M2DaTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELDSY1KSzVyPUOuPVAyPzhizszN MkDEV2FPT0WU
IST-MES1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPFd4ZKSzVyPUOuPVQxOjlizszN M{mzTHNCVkeHUh?=
NH-12 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELQOotKSzVyPUOuPVQyQDdizszN MYjTRW5ITVJ?
JVM-2 NVTPdIVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjJS|FKSzVyPUOuPVQ4PTJizszN MWHTRW5ITVJ?
EW-16 M1XyZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPrTWM2OD1|Lkm1OVc4KM7:TR?= NGS5NGFUSU6JRWK=
NCI-H441 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTNwOUewNVQh|ryP MYTTRW5ITVJ?
RCC10RGB MmryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHmPIZMUUN3ME20MlAyOjR4IN88US=> NEe5bJhUSU6JRWK=
TCCSUP Mn7US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTRwMUGwOUDPxE1? M1LweXNCVkeHUh?=
HL-60 NW\iSnNVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1n4TGlEPTB;ND6xNlI1OSEQvF2= NXTEc4NlW0GQR1XS
EW-11 NVvh[nU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTRwMUOyOlEh|ryP MYTTRW5ITVJ?
KARPAS-299 NVfLSIRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTRwM{GwNVMh|ryP MoDrV2FPT0WU
SNU-C2B MoDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\VRoQ5UUN3ME20MlM1Pjh4IN88US=> NFP1XXdUSU6JRWK=
LU-139 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjKPI1KSzVyPUSuN|czPDdizszN M4Li[HNCVkeHUh?=
HCC1937 NEHUO2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTRwM{e5PVMh|ryP NXjUbmZKW0GQR1XS
GR-ST NYfC[4NYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojHTWM2OD12LkSzNVgh|ryP M3u1Z3NCVkeHUh?=
SK-OV-3 M3K1e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTRwNE[wOFIh|ryP M3jiRnNCVkeHUh?=
A172 MlXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTRwNUW1NlEh|ryP NUHCUFBCW0GQR1XS
NB10 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33iN2lEPTB;ND61PVc5OSEQvF2= MoLrV2FPT0WU
P30-OHK NF\Sb5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTRwOEG5Nlkh|ryP MXjTRW5ITVJ?
KYSE-520 NWTlZWJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHYUnpKSzVyPUSuPFgxOThizszN NYT1O|FyW0GQR1XS
KNS-62 M17Pbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LuVGlEPTB;ND65NFgxPyEQvF2= NUnRbndLW0GQR1XS
ES1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnNfJU4UUN3ME20Mlk3ODN3IN88US=> NIfydItUSU6JRWK=
KOSC-2 Ml\JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTRwOUm5OVkh|ryP M13VfXNCVkeHUh?=
TE-9 NGXTTpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvHdGZKSzVyPUWuNFE2OjVizszN M{XLRnNCVkeHUh?=
NCI-H1838 M2rjZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIqxToZKSzVyPUWuNFIxPzZizszN MoT2V2FPT0WU
KP-N-YN Mn70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTVwMUK1PVEh|ryP NUDLdIt4W0GQR1XS
ALL-PO NVLQb5ZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTVwMkK1PFch|ryP M360d3NCVkeHUh?=
NOMO-1 Ml34S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fxRWlEPTB;NT6yN|E4PiEQvF2= NH;FcXFUSU6JRWK=
CGTH-W-1 NYOze3dmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\iO3BqUUN3ME21MlM5PTh2IN88US=> MnSwV2FPT0WU
ATN-1 NF\idlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzvTWM2OD13LkSxNVg1KM7:TR?= NFnwNopUSU6JRWK=
AGS NEnnOJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTVwNEm2NVgh|ryP NUG3bY1bW0GQR1XS
SW13 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\MTWM2OD13LkWzNlY2KM7:TR?= MX;TRW5ITVJ?
SW1990 M2rGfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTVwNkKyPFUh|ryP MknpV2FPT0WU
BFTC-905 M2DIdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLwTWM2OD13Lk[1PFc5KM7:TR?= MoDEV2FPT0WU
RMG-I Ml;sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTVwNkW5OFIh|ryP NHu1WW9USU6JRWK=
HD-MY-Z MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nZUWlEPTB;NT62PFU5OSEQvF2= NGD5RVlUSU6JRWK=
HCC1187 MmfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTVwN{GwN|Ih|ryP NFjDOnVUSU6JRWK=
HCC2998 M33mR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDoXllKSzVyPUWuO|E5PzdizszN MUDTRW5ITVJ?
RT-112 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTVwOUCyOFUh|ryP NVfae3pxW0GQR1XS
CAKI-1 NI[wRZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnOUWpKSzVyPU[uNFA6QDVizszN MVrTRW5ITVJ?
HDLM-2 NEKzRYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVz0UJhGUUN3ME22MlAyPDh7IN88US=> MmDYV2FPT0WU
TE-10 NILCbohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF[zdYJKSzVyPU[uNFQ5OzNizszN MlHaV2FPT0WU
KE-37 NHX0NIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTZwMEeyOVgh|ryP MWnTRW5ITVJ?
RCM-1 M1L0Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHiVlZrUUN3ME22MlA4PzF7IN88US=> MkPZV2FPT0WU
Mewo Mlr2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTZwMUG0PFch|ryP NX7rNG01W0GQR1XS
L-428 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVH6TXhyUUN3ME22MlE5ODN5IN88US=> M3TLVXNCVkeHUh?=
8-MG-BA MojyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7iW|NsUUN3ME22MlE6Ozd7IN88US=> M4H3OHNCVkeHUh?=
SK-MEL-28 MnK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvRTWM2OD14LkKwPVY4KM7:TR?= MnnUV2FPT0WU
VA-ES-BJ M4jHeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTZwM{C0OlIh|ryP M1i5[3NCVkeHUh?=
CAL-120 NV[yNnBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHT[mtKSzVyPU[uOFUyPjJizszN NEm3[|lUSU6JRWK=
GAMG MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfyR41KSzVyPU[uOVA3OiEQvF2= NHrLPYlUSU6JRWK=
MSTO-221H M3zzbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnsTWM2OD14LkWxNVch|ryP M{LY[HNCVkeHUh?=
GCT NEXH[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWL4cpViUUN3ME22MlU{Ozh5IN88US=> NF23cJhUSU6JRWK=
SH-4 M4rIeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTBTYVHUUN3ME22MlU5QDR2IN88US=> MojEV2FPT0WU
Calu-3 M3:zUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7WVXZHUUN3ME22MlY2PTZ6IN88US=> M3fZS3NCVkeHUh?=
SK-HEP-1 NYjjSJNoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVSyWpNqUUN3ME22MlcyPzV|IN88US=> MnzlV2FPT0WU
PFSK-1 Mli2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFryZ3pKSzVyPU[uO|M1PzhizszN M4D1fHNCVkeHUh?=
NB12 NHS3N2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTK[YRKSzVyPU[uO|g1ODRizszN NEfVVmJUSU6JRWK=
A388 NVrC[XBGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLLTFBKSzVyPU[uPFk2OiEQvF2= NWrWN251W0GQR1XS
KLE MkfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvXTWM2OD14Lkm2OVE3KM7:TR?= MXLTRW5ITVJ?
TE-11 NXLMW|JrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\oOYlWUUN3ME23MlA1QTZ5IN88US=> NWTld21nW0GQR1XS
NCI-H2405 NWTxSoU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rrW2lEPTB;Nz6wPFM6OyEQvF2= NED4ZpRUSU6JRWK=
OVCAR-4 NGSyOJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXTTWM2OD15LkG1N|Uh|ryP NXzldoxJW0GQR1XS
KP-N-YS MlLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUSybJJkUUN3ME23MlE5OTRizszN NIn3W3JUSU6JRWK=
NOS-1 M{PhVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTdwMkW4Nlgh|ryP MY\TRW5ITVJ?
DMS-114 MoniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFu2eIRKSzVyPUeuNlk3OzFizszN NGXtUnpUSU6JRWK=
NCI-H2030 NXHje4JUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTdwM{O2PVgh|ryP NGfQPZpUSU6JRWK=
HEL MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jHUGlEPTB;Nz6zO|UzOSEQvF2= NVHWbnM4W0GQR1XS
CAL-62 NVyyfVltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\pNGNKSzVyPUeuN|k5PjZizszN NY\aSItDW0GQR1XS
COLO-668 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zaVmlEPTB;Nz60N|k3PyEQvF2= M3jQ[XNCVkeHUh?=
SKG-IIIa NYWxdXBHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTdwNEWzPFEh|ryP MlHGV2FPT0WU
HCC1419 NVfMenZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LHdWlEPTB;Nz60Olg{OyEQvF2= NVnWTGkyW0GQR1XS
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NB14 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPsTWM2OD1|OD60OVQ1KM7:TR?= MW\TRW5ITVJ?
D-423MG MmPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vNZ2lEPTB;M{iuOFg3QSEQvF2= M2K5N3NCVkeHUh?=
NCI-H510A M3PZb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXQUGxKSzVyPUO4MlY1PTFizszN M{Ti[nNCVkeHUh?=
NMC-G1 NGDCNW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGToboFKSzVyPUO4MlczOjJizszN MlK2V2FPT0WU
SW948 M325Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrpeWFKSzVyPUO4Mlg6PDlizszN M3TKTnNCVkeHUh?=
MFM-223 NFvJZ|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnBeZdKSzVyPUO4Mlk1OzFizszN NWHxTpZSW0GQR1XS
PAN1 NUXzRY9jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjsTWM2OD1|OD65PVE6KM7:TR?= NGrkbWJUSU6JRWK=
MZ7-mel MljES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHMdpk{UUN3ME2zPU4yOTl7IN88US=> NF7TUVRUSU6JRWK=
COLO-679 NHLVToJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\rbGlEPTB;M{muPVcxQCEQvF2= NF[5doNUSU6JRWK=
SW872 NHXvdVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfCZXR5UUN3ME20NE4zODNzIN88US=> NYnVb3p1W0GQR1XS
HT-1197 MkXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTRyLkK4Olkh|ryP M{LDWnNCVkeHUh?=
ES6 MnfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYr1TnIxUUN3ME20NE41PTV{IN88US=> MlzZV2FPT0WU
SNU-387 NWC0W2tUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPSTWM2OD12MD64NlM5KM7:TR?= MUHTRW5ITVJ?
T47D MnLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DnemlEPTB;NEGuNFE6OiEQvF2= MV;TRW5ITVJ?
G-402 M3z3OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTRzLkC4NFkh|ryP MUnTRW5ITVJ?
MZ1-PC NHvnNm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELuNoNKSzVyPUSxMlgyKM7:TR?= NYPyTZNiW0GQR1XS
SW1417 NITEcVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjsZmNKSzVyPUSyMlMxOyEQvF2= MULTRW5ITVJ?
RERF-LC-MS MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTR{LkWwPFch|ryP MYLTRW5ITVJ?
SF539 NGPp[21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTR{LkW1Nlkh|ryP NXLhOXNPW0GQR1XS
ESS-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTR|LkW1OVgh|ryP NGnycVVUSU6JRWK=
RO82-W-1 NFHSfJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml2yTWM2OD12Mz62NVU{KM7:TR?= NUfIUWxJW0GQR1XS
S-117 Mnu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoL6TWM2OD12Mz62PVU5KM7:TR?= MlrTV2FPT0WU
SW756 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTR|LkiyOFIh|ryP MlHYV2FPT0WU
CP66-MEL M4HZR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3p[oJMUUN3ME20OU44OTR|IN88US=> NEDlXGpUSU6JRWK=
H-EMC-SS M17FZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnHTWM2OD12NT63Olk{KM7:TR?= MWLTRW5ITVJ?
NCI-H2052 M{Szc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfKbIRKSzVyPUS1MlkxOjdizszN M{\NfXNCVkeHUh?=
SW684 NUHCfmZET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTR5LkCwPFEh|ryP MVrTRW5ITVJ?
HCC1569 M1nnOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTR5LkG2OVgh|ryP M1GweXNCVkeHUh?=
KGN M4\FT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1O0[GlEPTB;NEeuN|MyOSEQvF2= MWPTRW5ITVJ?
LCLC-103H M{LMcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LaNmlEPTB;NEeuOFQxPCEQvF2= MVzTRW5ITVJ?
SW780 NHfxNlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrrUHltUUN3ME20O{43OzVzIN88US=> M{XNVHNCVkeHUh?=
NCI-H1703 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVP5cJVzUUN3ME20PE4{OzV{IN88US=> MkjxV2FPT0WU
HCC1395 MnnyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETZdnFKSzVyPUS4Mlc1OzJizszN NXnUdJhUW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03866499 Not yet recruiting NSCLC Beta Pharma Inc. May 2019 Phase 3
NCT03866499 Not yet recruiting NSCLC Beta Pharma Inc. May 2019 Phase 3
NCT03787992 Not yet recruiting Locally Advanced or Metastatic EGFR Sensitising Mutation Positive Non-small Cell Lung Cancer Allist Pharmaceuticals Inc. April 1 2019 Phase 3
NCT03787992 Not yet recruiting Locally Advanced or Metastatic EGFR Sensitising Mutation Positive Non-small Cell Lung Cancer Allist Pharmaceuticals Inc. April 1 2019 Phase 3
NCT03766490 Not yet recruiting Non-Small-Cell Lung The First Affiliated Hospital of Soochow University|The First People''s Hospital of Changzhou|Second Affiliated Hospital of Soochow University|Nantong University|Affiliated Hospital of Jiangnan University|Jiangyin People''s Hospital|Changzhou No.2 People''s Hospital|Chia Tai Tianqing Pharmaceutical Group Co. Ltd. March 30 2019 Not Applicable
NCT03856697 Not yet recruiting Advanced Non-small Cell Lung Cancer Hangzhou ACEA Pharmaceutical Research Co. Ltd.|Guangdong Provincial People''s Hospital March 2019 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID