Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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In DMSO USD 191 In stock
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Cited by 93 Publications

14 Customer Reviews

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moi2TWM2OD1yLkC1OEDPxE1? MlnKNVY2OTZ2N{O=
NIH3T3 M4HqdmZ2dmO2aX;uJGF{e2G7 MUewMlIh|ryP M2TsOlIhcA>? NVy0RoE1TE2VTx?= NHvqNGZKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? NE\LOoYyPjR6MEK4OC=>
COS7 M1rHZWZ2dmO2aX;uJGF{e2G7 NXTS[WZuQTZizszN NIW2WIhKdmirYnn0bY9vKG:oIHj1cYFvKEqDS{KgWlYyP0ZibYX0ZY51KGW6cILld5Nm\A>? NI\5dFMyPzF5OEeyNi=>
A549 MmSwR5l1d3SxeHnjJGF{e2G7 MXK5OkBp MXjEUXNQ MYTJR|UxRTF|LkW5JO69VQ>? M4LpZlE5OzF|OEC3
K562 M4PsOWN6fG:2b4jpZ{BCe3OjeR?= NE\XT2w6PiCq MUDEUXNQ MYPJR|UxRTlwM{[g{txO NYDlfYlSOTh|MUO4NFc>
MDA-MB-231 NHTEcnpEgXSxdH;4bYMhSXO|YYm= MnvLPVYhcA>? M4Cw[mROW09? MmLXTWM2OD1|Nj62NUDPxE1? M1XuW|E5OzF|OEC3
MOLT4 NY[yTG5NS3m2b4TvfIlkKEG|c3H5 M3jaPVk3KGh? M2LpTWROW09? NEDJbVNKSzVyPUG1MlAzKM7:TR?= NFPldmgyQDNzM{iwOy=>
MCF7 MXrDfZRwfG:6aXOgRZN{[Xl? NWXCdW9nPTBizszN Mmq0O|IhcA>? NUGyNlhFUUN3ME2xNk4xPSEQvF2= MoHtNVg4PzF6MUm=
BT474 M17xdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\lUmM4OiCq NGHUfYdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOFc1KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXSRoIzKHerdHigSWM2OCCxZjCwMlMh|ryP NIOySGsyQTB{OESyOC=>
HN5 NX\XcWpLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvmO|IhcA>? NXnpbGEySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUlUh[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkB4cXSqIFXDOVAhd2ZiMD6wNUDPxE1? NE\wWGwyQTB{OESyOC=>
BA/F3 MV;DfZRwfG:6aXOgRZN{[Xl? NIHqcm44OiCq MkjqR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCHR1\SJGw5PTiUIH31eIFvfCC5aYToJGlEPTBib3[gNE4xOTB6IN88US=> M2KzdFE6OjN7MkK5
NIH3T3 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGT0cJE4OiCq NUfiUoM2T3Kxd4ToJIlvcGmkaYTpc44hd2ZibX;1d4UhVkmKL{PUN{Bk\WyuczDlfJBz\XO|aX7nJIVzSjJiZ3Xu[UB4cXSqIFnDOVAhd2ZiMD61OEDPxE1? NFHL[VcyQTZ4NUO3Oy=>
MCF7 MX M3v2dGZ2dmO2aX;uJGF{e2G7 M1HzbVMxKG2rbh?= NXXjTmN2UW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4yKM7:TR?= M4fsflE6QTN{OU[w
A431 Ml3pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWCwOJBHOTBizszN MXy3NkBp M3;L[WdKPTB;MD61NVQh|ryP NX7zTYdvOTl7Nkm0OlU>
A431 MXHLbY5ie2ViQYPzZZk> MWSwMlEh|ryP NFnmcIIyKGh? M33mb2lvcGmkaYTpc44hd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> MYGxPVk3QTR4NR?=
A431 Mlr2T4lv[XOnIFHzd4F6 NUHxTGp5OTByIN88US=> MnTNNVAhdWmw M33YbXBzd2yxbnfl[EBqdmirYnn0c5J6KGGldHn2bZR6KG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v M1PHUlE6QTZ7NE[1
Sf9 MWHLbY5ie2ViQYPzZZk> Mn;tNVAhdWmw MoTRSG1UVw>? M1\QNGlvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= NEjoXm0zODB3NkSyOS=>
NCI-H1975 NUTRVJA{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYC1U45GPSEQvF2= MV:3NkBp NXTReVE2UUN3ME24MlI3KM7:TR?= M1LnOlIxOTVzNkew
NCI-H1975 M1SzWWtqdmG|ZTDBd5NigQ>? MkLBNVAh|ryP NGfieJNKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NIGxbmIzODF3MU[3NC=>
NCI-H1975 M4TkeGZ2dmO2aX;uJGF{e2G7 MXyxNEDPxE1? NYjnR2l7UW6qaXLpeIlwdiCxZjDFVmJDOiCjdYTvdIhwe3Cqb4L5cIF1cW:w NUT3dXFTOjBzNUG2O|A>
NCI-H1975 M1z3T2Z2dmO2aX;uJGF{e2G7 M1P3RVUh|ryP M{nIUVQ5KGh? M2Szbmlv\HWldHnvckBw\iCjcH;weI9{cXNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iClYYPwZZNmNTN? MnjPNlAyPTF4N{C=
A375 NYKyUI9RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHlRVJZPDhiaB?= MX\JR|UxRTJyLkW1JO69VQ>? M3S2TFIxOzB2NUO3
SMMC7721 NGraSFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HBSFQ5KGh? MWPJR|UxRTN|LkGyJO69VQ>? NE\OT3QzODNyNEWzOy=>
DU145 NWjJeGszS3m2b4TvfIlkKEG|c3H5 M3q0SlUh\A>? NX7lUmlPTE2VTx?= MX7JR|UxRTR{LkG3JO69VQ>? Mnm5NlA1PjZ3NUW=
MIAPaCa2 NXvWcWRHS3m2b4TvfIlkKEG|c3H5 MX:1JIQ> M2\CVWROW09? NWT5VWx1UUN3ME21NE43OiEQvF2= MWmyNFQ3PjV3NR?=
PANC1 NYn4[W17S3m2b4TvfIlkKEG|c3H5 NV7tR25CPSCm NFnTbmlFVVOR Mo[0TWM2OD12MD60NkDPxE1? MoC3NlA1PjZ3NUW=
PC9 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1S1PGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgSWdHWi2mZX\pZ4lmdnRiaIXtZY4hWEN7IHPlcIx{KHerdHigSWM2OCCxZjCwMlAyPyEQvF2= MljaNlEzODh6MEK=
PC9 M1LRWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= M4jSXFIyOjB6OECy
PC3 NULzW4ltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXuyVldxOTBizszN MUi3NkBp MmP5SG1UVw>? M4DFVWlEPTB;Nz60JO69VQ>? NYW1ZXN7OjF|NUO1OFY>
MDA-MB-468 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXuxNFAh|ryP M3rDWFQ5KGh? NEO3dYxFVVOR Mn:3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= NGPNfZczOTR{OU[zNi=>
Plasmodium falciparum M1XTWpFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w NHXGTIozOThzN{C0OS=>
NCI-H1993 NHewPZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DUb|czKGh? NEnuNHRFVVOR Ml\zTWM2OD1yLkGg{txO MUSyNVkyPjR|Mx?=
NCI-H292 NE\OdI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XTcFczKGh? MnPISG1UVw>? Mn7ITWM2OD1yLkCyJO69VQ>? M2OxfVIyQTF4NEOz
A431 MUXLbY5ie2ViQYPzZZk> Mn;MNkBp NXS4bGx[TE2VTx?= NGrue3FKdmirYnn0bY9vKG:oIFXHSnIheGixc4Doc5J6dGG2aX;uJIlvKEWJRj3zeIlufWyjdHXkJIh2dWGwIFG0N|Ehf2m2aDDJR|UxKG:oIECuNFAyKM7:TR?= NX:xS5JtOjJzMEGxN|I>
MCF7 NELhWYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2foWFExOMLizsznM41N M{KwbVQ5KGh? M4LHdGROW09? MlP5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN M2Prd|IzOTF7MUOw
BT474 M1\VfGN6fG:2b4jpZ{BCe3OjeR?= NF\BSnEyOCEQvF2= MWW0PEBp NI[5UG1EgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIFLUOFc1KGOnbHzz NXixVoVsOjJzM{izNFg>
MCF7 NGTKUZBEgXSxdH;4bYMhSXO|YYm= NWDyNY17OTBizszN NYHvbohuPDhiaB?= NWX5OZNyS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDNR2Y4KGOnbHzzJI93\XJvZYjwdoV{e2mwZzDFdoJDOiClZXzsdy=> NGTnOpYzOjF|OEOwPC=>
HCC827 M2PkbGtqdmG|ZTDBd5NigQ>? MUewMlEzPSEQvF2= NVTa[mFTOjRiaB?= NUDDRVk2TE2VTx?= NF[4bXZKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? MX2yNlM{QTN2Mh?=
HCC827 NX72WFZ{U2mwYYPlJGF{e2G7 M4K4SFAvOTJ3IN88US=> NV;x[3hPOjRiaB?= NV6xbpdYTE2VTx?= NYHqZmhNUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFXyb{BxcG:|cHjvdplt[XSrb36= M4HLe|IzOzN7M{Sy
HCC827 MnSwT4lv[XOnIFHzd4F6 NFznXWIxNjF{NTFOwG0> MVOyOEBp MWPEUXNQ MVLJcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhSWu2IIDoc5NxcG:{eXzheIlwdg>? M2\ZOVIzOzN7M{Sy
A431 NV\qVllmU2mwYYPlJGF{e2G7 MkLzNVAxKM7:TR?= MmS4N|AhdWmw NFfP[I1KdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN MViyNlY6QDd6Mh?=
H460 MXrDfZRwfG:6aXOgRZN{[Xl? Mn;5O|IhcA>? MonRTWM2OD13LkW5JO69VQ>? MkDuNlI4Ojd2NEm=
HT-29 NGnjTmNEgXSxdH;4bYMhSXO|YYm= NFTMZoQ4OiCq MnzrTWM2OD1|LkO2JO69VQ>? MVOyNlczPzR2OR?=
SGC7901 MX7DfZRwfG:6aXOgRZN{[Xl? NIHXPIU4OiCq M{W3c2lEPTB;MUCuNlYh|ryP NXS5TWtnOjJ5Mke0OFk>
A498 MkD5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3MelJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= MUGyNlgyQDh2OB?=
Calu3 NVLjWHlpS3m2b4TvfIlkKEG|c3H5 MkWxNVAh|rypL33M Mo[zO|IhcA>? NXzP[ItlUUN3ME2xMlYh|ryP NE\U[WEzOzFzNkG2PC=>
FADU MVzDfZRwfG:6aXOgRZN{[Xl? MXexNEDPxGdxbVy= NIjvN2Y4OiCq M{Xse2lEPTB;MD6wOUDPxE1? MUGyN|EyPjF4OB?=
HCC827 Mnf3R5l1d3SxeHnjJGF{e2G7 MVmxNEDPxGdxbVy= M3HHZVczKGh? NG\xb5FKSzVyPUCuNFAyPSEQvF2= NHj2PY0zOzFzNkG2PC=>
PC9 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7QNVAh|rypL33M M1rLV|czKGh? NWrv[GtxUUN3ME2wMlAxOTRizszN MYWyN|EyPjF4OB?=
Sf9 NYf3foVmU2mwYYPlJGF{e2G7 M1PiW|EhcA>? NF\DZVhFVVOR NXHUd5JHUW6qaXLpeIlwdiCxZjDoeY1idiCJU2SteIFo\2WmIFXHSnIhVDh|NGKgcZV1[W62IHX4dJJme3OnZDD3bZRpKEmFNUCgc4YhOC5yMUWg{txO NWGzdHhSOjN4MUG2PVE>
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A498 MorsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTJwMkm0NVUh|ryP NITmXIFUSU6JRWK=
IGROV-1 NWnnNXFDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{eze2lEPTB;Mj6zNVI{PyEQvF2= NFX3OYhUSU6JRWK=
JVM-3 NEnCTXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M121c2lEPTB;Mj6zOFY1OyEQvF2= MVzTRW5ITVJ?
NCI-H2170 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojrTWM2OD1{LkO0O|g{KM7:TR?= MmflV2FPT0WU
EW-18 NWLCXXlpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPuTWM2OD1{LkO1OFcyKM7:TR?= MXrTRW5ITVJ?
KU812 NFLkTm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlH4TWM2OD1{LkO2O|c6KM7:TR?= MVvTRW5ITVJ?
NY MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrWTWM2OD1{LkO3Nlch|ryP NEX3OIZUSU6JRWK=
HTC-C3 MkLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvvTWM2OD1{LkSyNlY4KM7:TR?= MlzPV2FPT0WU
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HH MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPlTWM2OD1{LkS5PFU1KM7:TR?= NIrI[WZUSU6JRWK=
MKN7 M1zNTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnj[HZMUUN3ME2yMlYzPzR6IN88US=> NV3COWtEW0GQR1XS
TE-8 NUfnd5RwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\MZ2lEPTB;Mj62N|Y2OyEQvF2= MULTRW5ITVJ?
MC-IXC M1PTeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXMcohKSzVyPUKuOlQ1PTNizszN NYC3ZVRLW0GQR1XS
DMS-79 NY\zW3B{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrhSYV1UUN3ME2yMlY6Ojh2IN88US=> M1GzfHNCVkeHUh?=
Detroit562 NFvPU2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPJTWM2OD1{Lk[5PFQ2KM7:TR?= NHTCbFZUSU6JRWK=
HuP-T4 Ml2xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHFe|dpUUN3ME2yMlczQDF6IN88US=> MkS4V2FPT0WU
QIMR-WIL NIfNW2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXGPYxKSzVyPUKuO|U6PTJizszN NHHTbXlUSU6JRWK=
BB65-RCC NH[yXGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIL3[ZVKSzVyPUKuPFA4OTNizszN NFjSSmJUSU6JRWK=
PC-3 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{L6[2lEPTB;Mj64N|MyPyEQvF2= NXvkUIVJW0GQR1XS
OVCAR-3 NYXUOWVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HHR2lEPTB;Mj64OVU6PCEQvF2= NIfESVRUSU6JRWK=
KYSE-510 NUPtVJlOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\m[2FKSzVyPUKuPFY4QDZizszN NVniO45LW0GQR1XS
HC-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\yVnVKSzVyPUKuPVQxOTJizszN Ml7UV2FPT0WU
NCI-H1693 NWH6WoVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPhTWM2OD1{Lkm1PFk4KM7:TR?= NVTvb4RVW0GQR1XS
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COLO-680N M4rlWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;kOIExUUN3ME2zMlAxPDl3IN88US=> NXnGZZlbW0GQR1XS
MN-60 NGOzXJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfkc4VKSzVyPUOuNFQ6PzRizszN MUPTRW5ITVJ?
BCPAP M{fzfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn35TWM2OD1|LkC4Nlk2KM7:TR?= MnXJV2FPT0WU
647-V MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrJXlVRUUN3ME2zMlE3OjJizszN MV;TRW5ITVJ?
HT-1376 M{XJdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFT1R|BKSzVyPUOuNlA2ODZizszN NWrMVohIW0GQR1XS
NCI-H1793 M2DIXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3j2bWlEPTB;Mz6yNVA6QCEQvF2= M1HTNHNCVkeHUh?=
SW1463 M2PoZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTZTWM2OD1|LkK2NVkzKM7:TR?= Mni0V2FPT0WU
NCI-H727 Mm\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4L5XmlEPTB;Mz6zPFU5PCEQvF2= NF[ybFZUSU6JRWK=
LB771-HNC Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDLTHc6UUN3ME2zMlM6OTJ4IN88US=> M1q3NHNCVkeHUh?=
D-542MG MkPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHsZZBKSzVyPUOuOFQzOjJizszN NEDPc4lUSU6JRWK=
NTERA-S-cl-D1 M{j0bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHoemRlUUN3ME2zMlQ1QTV5IN88US=> MnTiV2FPT0WU
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639-V MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEW1R5ZKSzVyPUOuOVc4QTJizszN M3LLfXNCVkeHUh?=
TE-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13RSmlEPTB;Mz61PVE3QSEQvF2= NXO5T5JsW0GQR1XS
OAW-28 M2qzZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1[0cGlEPTB;Mz61PVQzPiEQvF2= M2P1XHNCVkeHUh?=
SK-LU-1 M1zMN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;CTWM2OD1|Lk[wOlQ2KM7:TR?= M3rlcnNCVkeHUh?=
CaR-1 NETOc49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHS0[FdKSzVyPUOuOlExPDhizszN NVzPRWVPW0GQR1XS
A2780 M2rkcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\IOZRzUUN3ME2zMlY1ODh7IN88US=> M1O1[HNCVkeHUh?=
C2BBe1 MljqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV75N4FuUUN3ME2zMlY1PDB5IN88US=> MX7TRW5ITVJ?
SK-MES-1 M{Ozd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlmxTWM2OD1|Lk[0OlE1KM7:TR?= MX3TRW5ITVJ?
PANC-10-05 NGTPRZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTNwN{KwO|Ih|ryP NFn4bZlUSU6JRWK=
J-RT3-T3-5 M{XGSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7LTWM2OD1|Lke1OFA3KM7:TR?= M1XafnNCVkeHUh?=
ES4 M1XsN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPiXJRzUUN3ME2zMlgzOjh4IN88US=> M2\ifnNCVkeHUh?=
NCI-H28 NXL4cZFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTNwOEK5OlEh|ryP MkSxV2FPT0WU
NCI-H1155 M4Pxemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTNwOESxOVkh|ryP MXLTRW5ITVJ?
KURAMOCHI M{OyZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mle3TWM2OD1|Lki2NFU3KM7:TR?= NEm1XWNUSU6JRWK=
NCI-H69 MlfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jyZmlEPTB;Mz65NFE4QCEQvF2= NYC5T41oW0GQR1XS
IST-MES1 NF;nSJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\vTWM2OD1|Lkm0NFI6KM7:TR?= MVTTRW5ITVJ?
NH-12 NUDybW04T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjqU4tUUUN3ME2zMlk1OTh5IN88US=> M3vvS3NCVkeHUh?=
JVM-2 MnfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvMVI5yUUN3ME2zMlk1PzV{IN88US=> M{XzdHNCVkeHUh?=
EW-16 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLITWM2OD1|Lkm1OVc4KM7:TR?= M4X5dXNCVkeHUh?=
NCI-H441 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HTcGlEPTB;Mz65O|AyPCEQvF2= NVjCU3BTW0GQR1XS
RCC10RGB NHnNUnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUG0[|Q5UUN3ME20MlAyOjR4IN88US=> M3TC[nNCVkeHUh?=
TCCSUP MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUS5PWdPUUN3ME20MlEyODVizszN NGn1TnRUSU6JRWK=
HL-60 NUH6V2hlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmL4TWM2OD12LkGyNlQyKM7:TR?= MoDJV2FPT0WU
EW-11 NGTRNHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTFTWM2OD12LkGzNlYyKM7:TR?= NHjzVVJUSU6JRWK=
KARPAS-299 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPxb2xKSzVyPUSuN|ExOTNizszN MoH6V2FPT0WU
SNU-C2B MnTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2L2PWlEPTB;ND6zOFY5PiEQvF2= NWLz[mZkW0GQR1XS
LU-139 M3LHWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTBTWM2OD12LkO3NlQ4KM7:TR?= NWrobZR2W0GQR1XS
HCC1937 NXXOboQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjDTWM2OD12LkO3PVk{KM7:TR?= MYrTRW5ITVJ?
GR-ST Mn;kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLMU41KSzVyPUSuOFMyQCEQvF2= NWTGS203W0GQR1XS
SK-OV-3 NEHtSlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHaTVUxUUN3ME20MlQ3ODR{IN88US=> Mn\EV2FPT0WU
A172 NX\QOXRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1KwPGlEPTB;ND61OVUzOSEQvF2= M{W3eHNCVkeHUh?=
NB10 Ml;qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXBUINpUUN3ME20MlU6PzhzIN88US=> NWnIRYRoW0GQR1XS
P30-OHK NUTzOGxpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTRwOEG5Nlkh|ryP NH74co5USU6JRWK=
KYSE-520 M4XOUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLubWdVUUN3ME20Mlg5ODF6IN88US=> NVTuVFJxW0GQR1XS
KNS-62 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3joWWlEPTB;ND65NFgxPyEQvF2= MYHTRW5ITVJ?
ES1 MlTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHyyNHJKSzVyPUSuPVYxOzVizszN NFPUc3BUSU6JRWK=
KOSC-2 M{G5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTRwOUm5OVkh|ryP NYTxPHBoW0GQR1XS
TE-9 MlnwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfJTWM2OD13LkCxOVI2KM7:TR?= Mmn2V2FPT0WU
NCI-H1838 NVfLfJRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHu5RnJKSzVyPUWuNFIxPzZizszN MnLrV2FPT0WU
KP-N-YN NH\QdmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPMZXhuUUN3ME21MlEzPTlzIN88US=> MorKV2FPT0WU
ALL-PO NFjQfZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTVwMkK1PFch|ryP M{i4bHNCVkeHUh?=
NOMO-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTTemV2UUN3ME21MlI{OTd4IN88US=> MYPTRW5ITVJ?
CGTH-W-1 NX3UNY05T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPDU2lKSzVyPUWuN|g2QDRizszN MXnTRW5ITVJ?
ATN-1 MlLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7pWFJ6UUN3ME21MlQyOTh2IN88US=> MUnTRW5ITVJ?
AGS Mn3GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTjO21{UUN3ME21MlQ6PjF6IN88US=> MoHDV2FPT0WU
SW13 Ml7oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzLR3NKSzVyPUWuOVMzPjVizszN M3jxT3NCVkeHUh?=
SW1990 NUjPOGFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vjOmlEPTB;NT62NlI5PSEQvF2= MmqzV2FPT0WU
BFTC-905 NIfifJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTVwNkW4O|gh|ryP MVXTRW5ITVJ?
RMG-I NVLUNI41T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrzTWM2OD13Lk[1PVQzKM7:TR?= NH\4dYlUSU6JRWK=
HD-MY-Z MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHhOIVKSzVyPUWuOlg2QDFizszN M1TtXHNCVkeHUh?=
HCC1187 M{P5Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHBTWM2OD13LkexNFMzKM7:TR?= NVrXUo1nW0GQR1XS
HCC2998 M2rvOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LRUGlEPTB;NT63NVg4PyEQvF2= MX3TRW5ITVJ?
RT-112 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PtTWlEPTB;NT65NFI1PSEQvF2= M{Gx[XNCVkeHUh?=
CAKI-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTZwMEC5PFUh|ryP MmrWV2FPT0WU
HDLM-2 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPETWM2OD14LkCxOFg6KM7:TR?= MYXTRW5ITVJ?
TE-10 NF3zSHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\LR2x6UUN3ME22MlA1QDN|IN88US=> NIq5UmFUSU6JRWK=
KE-37 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTZwMEeyOVgh|ryP NEG3TZFUSU6JRWK=
RCM-1 NUnZR5o5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWr1OFhqUUN3ME22MlA4PzF7IN88US=> NH3uNJFUSU6JRWK=
Mewo MlPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXlUHRXUUN3ME22MlEyPDh5IN88US=> M3niTnNCVkeHUh?=
L-428 NFLSRWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkL5TWM2OD14LkG4NFM4KM7:TR?= NETHU4RUSU6JRWK=
8-MG-BA MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TPW2lEPTB;Nj6xPVM4QSEQvF2= Mn7YV2FPT0WU
SK-MEL-28 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTZwMkC5Olch|ryP M{DMdnNCVkeHUh?=
VA-ES-BJ Mn\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7GbYlzUUN3ME22MlMxPDZ{IN88US=> MX3TRW5ITVJ?
CAL-120 NHX4OFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVe0bXJIUUN3ME22MlQ2OTZ{IN88US=> NYSzWW5LW0GQR1XS
GAMG M1vxN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvn[JVKSzVyPU[uOVA3OiEQvF2= MW\TRW5ITVJ?
MSTO-221H MoK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHIbJJKSzVyPU[uOVEyPyEQvF2= MkLmV2FPT0WU
GCT MlPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HM[mlEPTB;Nj61N|M5PyEQvF2= NIDxPHJUSU6JRWK=
SH-4 NF\GeVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmr3TWM2OD14LkW4PFQ1KM7:TR?= M{j6WXNCVkeHUh?=
Calu-3 MoDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTSfZJJUUN3ME22MlY2PTZ6IN88US=> NFOwUZRUSU6JRWK=
SK-HEP-1 NXnWVpBpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTZwN{G3OVMh|ryP MknDV2FPT0WU
PFSK-1 MnmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPIZmhKSzVyPU[uO|M1PzhizszN NW[2bXRMW0GQR1XS
NB12 M13jS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTZwN{i0NFQh|ryP MVPTRW5ITVJ?
A388 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjoeIxKSzVyPU[uPFk2OiEQvF2= M1vvWXNCVkeHUh?=
KLE MmHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnmOIxKSzVyPU[uPVY2OTZizszN MWjTRW5ITVJ?
TE-11 NGjI[5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\1TWM2OD15LkC0PVY4KM7:TR?= NFvxNVRUSU6JRWK=
NCI-H2405 M4G5e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTdwMEizPVMh|ryP M{TQWnNCVkeHUh?=
OVCAR-4 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYW1UodtUUN3ME23MlE2OzVizszN NVP5TFlOW0GQR1XS
KP-N-YS MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlG4TWM2OD15LkG4NVQh|ryP NHq5NVVUSU6JRWK=
NOS-1 NEfI[XRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnxVm1KSzVyPUeuNlU5OjhizszN NFjHfW1USU6JRWK=
DMS-114 NGKw[WdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvmTnZKSzVyPUeuNlk3OzFizszN M2XsUHNCVkeHUh?=
NCI-H2030 NFHkWpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3ThfGlEPTB;Nz6zN|Y6QCEQvF2= M3S3SHNCVkeHUh?=
HEL NXT5ZmtnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnrfmJKSzVyPUeuN|c2OjFizszN NGG2N4JUSU6JRWK=
CAL-62 M1LkOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7uZoVOUUN3ME23MlM6QDZ4IN88US=> MlPpV2FPT0WU
COLO-668 MoX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTdwNEO5Olch|ryP MmDRV2FPT0WU
SKG-IIIa NHf2PWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTxN28zUUN3ME23MlQ2OzhzIN88US=> M3LlfXNCVkeHUh?=
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SW626 M{nLRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rOdmlEPTB;M{euNFQyPyEQvF2= NVrLboR6W0GQR1XS
ONS-76 Moe2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlm5TWM2OD1|Nz6xN|A6KM7:TR?= NE\xWHNUSU6JRWK=
T98G M{Hmfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TUXGlEPTB;M{euPFY{PSEQvF2= MnT5V2FPT0WU
NB14 NVm1XYdLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\aTWM2OD1|OD60OVQ1KM7:TR?= NGfJZ3RUSU6JRWK=
D-423MG NEn0TIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2X0NGlEPTB;M{iuOFg3QSEQvF2= NX[x[mtzW0GQR1XS
NCI-H510A NEnENY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXwbYlKSzVyPUO4MlY1PTFizszN NYfrfpNmW0GQR1XS
NMC-G1 NYqyd2JDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vuNmlEPTB;M{iuO|IzOiEQvF2= MWPTRW5ITVJ?
SW948 NW\hblhqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;nZWlEPTB;M{iuPFk1QSEQvF2= M1T2cXNCVkeHUh?=
MFM-223 NUGweppjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4K2XGlEPTB;M{iuPVQ{OSEQvF2= MYDTRW5ITVJ?
PAN1 M1rsWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfRTWM2OD1|OD65PVE6KM7:TR?= MnzwV2FPT0WU
MZ7-mel MlfJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vHWGlEPTB;M{muNVE6QSEQvF2= NILPUoRUSU6JRWK=
COLO-679 MmDLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3pNVZKSzVyPUO5Mlk4ODhizszN NIrnOW1USU6JRWK=
SW872 NXm0PJF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvhWYFKSzVyPUSwMlIxOzFizszN MlHOV2FPT0WU
HT-1197 NGfzc3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTRyLkK4Olkh|ryP NGfCUI5USU6JRWK=
ES6 NFu0VoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\yXmlEPTB;NECuOFU2OiEQvF2= NXTjdWtIW0GQR1XS
SNU-387 MlPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PkNWlEPTB;NECuPFI{QCEQvF2= M1fW[HNCVkeHUh?=
T47D MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXoNGxKSzVyPUSxMlAyQTJizszN MUHTRW5ITVJ?
G-402 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTRzLkC4NFkh|ryP M4LiS3NCVkeHUh?=
MZ1-PC NWPFb5JNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTRzLkixJO69VQ>? M3HRVnNCVkeHUh?=
SW1417 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTR{LkOwN{DPxE1? NYj5fXhDW0GQR1XS
RERF-LC-MS MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnFVnBKSzVyPUSyMlUxQDdizszN MlHQV2FPT0WU
SF539 NWnTS|VIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTR{LkW1Nlkh|ryP M13tWnNCVkeHUh?=
ESS-1 M2TzVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjQV3pTUUN3ME20N{42PTV6IN88US=> MUnTRW5ITVJ?
RO82-W-1 NH3KeodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnmxTWM2OD12Mz62NVU{KM7:TR?= NGj3fHdUSU6JRWK=
S-117 Mn25S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPGfnRKSzVyPUSzMlY6PThizszN MXzTRW5ITVJ?
SW756 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{D3RWlEPTB;NEOuPFI1OiEQvF2= MXvTRW5ITVJ?
CP66-MEL NVPjcVl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2X4dmlEPTB;NEWuO|E1OyEQvF2= MmHjV2FPT0WU
H-EMC-SS MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7C[G1KSzVyPUS1Mlc3QTNizszN NI\BflBUSU6JRWK=
NCI-H2052 NIrDO3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2n1U2lEPTB;NEWuPVAzPyEQvF2= M4\0OXNCVkeHUh?=
SW684 MkTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\2[IZKSzVyPUS3MlAxQDFizszN NXPsR|NWW0GQR1XS
HCC1569 NWjhNFM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHSdXBKSzVyPUS3MlE3PThizszN NFKyTVdUSU6JRWK=
KGN MkfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfrTo5qUUN3ME20O{4{OzFzIN88US=> MkDUV2FPT0WU
LCLC-103H MlroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mni0TWM2OD12Nz60OFA1KM7:TR?= MVnTRW5ITVJ?
SW780 MlTPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHKUIZKSzVyPUS3MlY{PTFizszN NEHlfXBUSU6JRWK=
NCI-H1703 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zwVWlEPTB;NEiuN|M2OiEQvF2= M{LCdnNCVkeHUh?=
HCC1395 MkXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vIOGlEPTB;NEiuO|Q{OiEQvF2= MnznV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

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    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03122717 Recruiting Non-Small Cell Lung Cancer Dana-Farber Cancer Institute|AstraZeneca May 9 2017 Phase 1
NCT00025675 Completed Brain and Central Nervous System Tumors Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins|National Cancer Institute (NCI) October 9 2001 Phase 2
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co. Ltd. September 8 2016 Phase 1
NCT01933347 Active not recruiting Non Small Cell Lung Cancer Guangdong Association of Clinical Trials April 7 2014 Phase 2
NCT03486496 Not yet recruiting Lung Adenocarcinoma|EGFR Mutation Fujian Cancer Hospital June 5 2018 Phase 2
NCT01610336 Active not recruiting Non-small Cell Lung Cancer Novartis Pharmaceuticals|Novartis April 5 2012 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID