Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 95 Publications

14 Customer Reviews

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MorOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfhTWM2OD1yLkC1OEDPxE1? NHP4T28yPjVzNkS3Ny=>
NIH3T3 NWnQUVN{TnWwY4Tpc44hSXO|YYm= NF:4R2ExNjJizszN M1r5VlIhcA>? Mlz6SG1UVw>? NEfnTmVKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? NXjD[3QyOTZ2OECyPFQ>
COS7 MmP6SpVv[3Srb36gRZN{[Xl? MWW5OkDPxE1? MonQTY5pcWKrdHnvckBw\iCqdX3hckBLSUt{IG[2NVdHKG23dHHueEBmgHC{ZYPz[YQ> M2S3flE4OTd6N{Ky
A549 M{ewNGN6fG:2b4jpZ{BCe3OjeR?= NVzjZpc3QTZiaB?= MXzEUXNQ NYLUTXZRUUN3ME2xN{42QSEQvF2= MUGxPFMyOzhyNx?=
K562 NHPmU2NEgXSxdH;4bYMhSXO|YYm= M1K4SVk3KGh? MXLEUXNQ MYjJR|UxRTlwM{[g{txO MUexPFMyOzhyNx?=
MDA-MB-231 MVXDfZRwfG:6aXOgRZN{[Xl? MXW5OkBp M4rrVmROW09? NGnFeoRKSzVyPUO2MlYyKM7:TR?= M1\i[|E5OzF|OEC3
MOLT4 MVfDfZRwfG:6aXOgRZN{[Xl? NH[0RXg6PiCq MWXEUXNQ MVfJR|UxRTF3LkCyJO69VQ>? MVmxPFMyOzhyNx?=
MCF7 M{LPW2N6fG:2b4jpZ{BCe3OjeR?= NXnZU5M2PTBizszN MUW3NkBp MlX4TWM2OD1zMj6wOUDPxE1? NF2z[JEyQDd5MUixPS=>
BT474 NFjPUXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWO1[XBCPzJiaB?= MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWNEe0JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWUQnKyJJdqfGhiRVO1NEBw\iByLkOg{txO NFnnRmcyQTB{OESyOC=>
HN5 NITJXXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWe3NkBp M3;sdGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO MYOxPVAzQDR{NB?=
BA/F3 M2DpPGN6fG:2b4jpZ{BCe3OjeR?= NV73VG8xPzJiaB?= MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDCRU9HOyClZXzsd{BmgHC{ZYPzbY5oKEWJRmKgUFg2QFJibYX0ZY51KHerdHigTWM2OCCxZjCwMlAyODhizszN MX6xPVI{QTJ{OR?=
NIH3T3 NXyyfYxCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrCO|IhcA>? MlHQS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> M1i2V|E6PjZ3M{e3
MCF7 MX NWDTWWpxTnWwY4Tpc44hSXO|YYm= M33lVFMxKG2rbh?= MU\Jcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJJdqfGhiSVO1NEBw\iBzLkGg{txO NInmWJAyQTl|Mkm2NC=>
A431 NV;q[5JOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLqd4cyOCEQvF2= M3XzRlczKGh? MX3HTVUxRTBwNUG0JO69VQ>? M1rOcFE6QTZ7NE[1
A431 M4S5XGtqdmG|ZTDBd5NigQ>? MoXDNE4yKM7:TR?= M37EdFEhcA>? MX\Jcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u MVuxPVk3QTR4NR?=
A431 NYTZXZZtU2mwYYPlJGF{e2G7 NVSxNGd1OTByIN88US=> MX:xNEBucW5? MmTNVJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> NW\WRWZqOTl7Nkm0OlU>
Sf9 NIm1bHNMcW6jc3WgRZN{[Xl? NYrKVXpiOTBibXnu NVPSXIVDTE2VTx?= NXHDOHZ2UW6qaXLpeIlwdiCxZjDFS2ZTKGW6cILld5Nm\CCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHKnY3XweI9zKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODN|IN88US=> NGfWUZAzODB3NkSyOS=>
NCI-H1975 M4\ocmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2r6OFUh|ryP M1S0d|czKGh? NFq1cmNKSzVyPUiuNlYh|ryP MmW5NlAyPTF4N{C=
NCI-H1975 NVOxUGJPU2mwYYPlJGF{e2G7 MX2xNEDPxE1? NHrGemtKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NIW0dY0zODF3MU[3NC=>
NCI-H1975 NHfmTWpHfW6ldHnvckBCe3OjeR?= Ml3ONVAh|ryP NEDDWpFKdmirYnn0bY9vKG:oIFXSRmIzKGG3dH;wbI9{eGixconsZZRqd25? MUSyNFE2OTZ5MB?=
NCI-H1975 MknRSpVv[3Srb36gRZN{[Xl? M1vs[|Uh|ryP NEDLNJo1QCCq MWPJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4Yh[2G|cHHz[U0{ MnzaNlAyPTF4N{C=
A375 M4DIWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFm4WoI1QCCq MXvJR|UxRTJyLkW1JO69VQ>? M2\IVVIxOzB2NUO3
SMMC7721 M2f2PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzFTHM1QCCq NUnqSXVWUUN3ME2zN{4yOiEQvF2= M{Sxe|IxOzB2NUO3
DU145 M1HVNWN6fG:2b4jpZ{BCe3OjeR?= MWi1JIQ> MlTpSG1UVw>? M4Pj[GlEPTB;NEKuNVch|ryP M161TFIxPDZ4NUW1
MIAPaCa2 M1HVSWN6fG:2b4jpZ{BCe3OjeR?= NFHR[4U2KGR? NXz1UoE4TE2VTx?= MnyzTWM2OD13MD62NkDPxE1? MXyyNFQ3PjV3NR?=
PANC1 MXfDfZRwfG:6aXOgRZN{[Xl? MlewOUBl Ml:xSG1UVw>? NVrkTYJoUUN3ME20NE41OiEQvF2= M{TrZVIxPDZ4NUW1
PC9 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDOXZVzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGT0[ULXTl[olkcWWwdDDoeY1idiCSQ{mgZ4VtdHNid3n0bEBGSzVyIH;mJFAvODF5IN88US=> NVjh[IFQOjF{MEi4NFI>
PC9 NVfXXIhqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYD1R45KSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBoem:5dHitdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDlfJBz\XO|aX7nJGVITlJiRUe0On9CPzVyL2S3PVBOKG23dHHueEB4cXSqIFXDOVAhd2ZiNzFOwG0> NWC2bZVuOjF{MEi4NFI>
PC3 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkK1NVAh|ryP MUC3NkBp NUjDRmpiTE2VTx?= MkPxTWM2OD15LkSg{txO NYXYS4RxOjF|NUO1OFY>
MDA-MB-468 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PpZlExOCEQvF2= MlTyOFghcA>? NXX0[245TE2VTx?= NWHlNYF[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NEnHZ4IzOTR{OU[zNi=>
Plasmodium falciparum Mkn4dWhVWyCycn;mbYxqdmdiZn;yJIlvcGmkaYTvdpMhd2ZiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJJBzd2yrZnXyZZRqd25? MYWyNVgyPzB2NR?=
NCI-H1993 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfxfGFOPzJiaB?= NYnWdVNwTE2VTx?= NE\ld3FKSzVyPUCuNUDPxE1? NY[1fmwxOjF7MU[0N|M>
NCI-H292 Mn\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfiXJo4OiCq MW\EUXNQ NFH6Z5BKSzVyPUCuNFIh|ryP NUnaeppOOjF7MU[0N|M>
A431 MnPIT4lv[XOnIFHzd4F6 NFPpNnUzKGh? MmfSSG1UVw>? M2D3dGlvcGmkaYTpc44hd2ZiRVfGVkBxcG:|cHjvdplt[XSrb36gbY4hTUeILYP0bY12dGG2ZXSgbJVu[W5iQUSzNUB4cXSqIFnDOVAhd2ZiMD6wNFEh|ryP M3vOW|IzOTBzMUOy
MCF7 NV;QfVZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXGxNFDDqM7:Zz;tUC=> MnnWOFghcA>? NHnrbXpFVVOR MmLRRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN NF6xV4YzOjFzOUGzNC=>
BT474 M{fseWN6fG:2b4jpZ{BCe3OjeR?= NEnUTlUyOCEQvF2= NXzqW5p{PDhiaB?= NGXYR45EgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIFLUOFc1KGOnbHzz Mk\GNlIyOzh|MEi=
MCF7 MmTnR5l1d3SxeHnjJGF{e2G7 MU[xNEDPxE1? MUG0PEBp MnrlR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCPQ1[3JINmdGy|IH;2[ZIu\XiycnXzd4lv\yCHcnLCNkBk\Wyucx?= NHjZUngzOjF|OEOwPC=>
HCC827 NWPSR2pYU2mwYYPlJGF{e2G7 NYDRVpJVOC5zMkWg{txO NFfaVnczPCCq NUnSfVRXTE2VTx?= M{XzO2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v NF\CT5czOjN|OUO0Ni=>
HCC827 M3[3eGtqdmG|ZTDBd5NigQ>? NEjIUVYxNjF{NTFOwG0> MYGyOEBp NFLySnhFVVOR NUX3WopRUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFXyb{BxcG:|cHjvdplt[XSrb36= NVTsWXBoOjJ|M{mzOFI>
HCC827 MX\LbY5ie2ViQYPzZZk> MVOwMlEzPSEQvF2= MYCyOEBp M{niNmROW09? NWjmNohqUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= MVGyNlM{QTN2Mh?=
A431 MYjLbY5ie2ViQYPzZZk> MX:xNFAh|ryP MVGzNEBucW5? MVvJcohq[mm2aX;uJI9nKEWJRmKg[IVzcX[nZDDmdo9uKGi3bXHuJGE1OzFiY3XscEBu\W2kcnHu[UBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJBwdHlvR3z1MXR6eiCyaH;zdIhwenmuYYTpc44hf2m2aDDJR|UxKG:oIEGuNFgh|ryP M{nxd|IzPjl6N{iy
H460 MYHDfZRwfG:6aXOgRZN{[Xl? M4jmOFczKGh? MojHTWM2OD13LkW5JO69VQ>? MljMNlI4Ojd2NEm=
HT-29 MmTXR5l1d3SxeHnjJGF{e2G7 M1f0[|czKGh? M1\IOGlEPTB;Mz6zOkDPxE1? NGWwWHEzOjd{N{S0PS=>
SGC7901 MVLDfZRwfG:6aXOgRZN{[Xl? MWK3NkBp MYHJR|UxRTFyLkK2JO69VQ>? MWeyNlczPzR2OR?=
A498 NFnLVIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPEVItuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFk5KGOnbHzzJIV5eHKnc4PpcochTUeIUjDhcoQhW1KFIIfpeIghUUN3MDDv[kA5NjZizszN M4\Lc|IzQDF6OES4
Calu3 M3vYdWN6fG:2b4jpZ{BCe3OjeR?= NWjWbJJiOTBizsznM41N NFjSeoI4OiCq MXjJR|UxRTFwNjFOwG0> M2T1dlI{OTF4MU[4
FADU NEHmR49EgXSxdH;4bYMhSXO|YYm= NYD3d5dLOTBizsznM41N MlziO|IhcA>? MnfOTWM2OD1yLkC1JO69VQ>? MXeyN|EyPjF4OB?=
HCC827 NUK3b4JES3m2b4TvfIlkKEG|c3H5 MWKxNEDPxGdxbVy= MkXuO|IhcA>? NF7sUm9KSzVyPUCuNFAyPSEQvF2= M{i3S|I{OTF4MU[4
PC9 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3rNVAh|rypL33M MUe3NkBp M{[wdWlEPTB;MD6wNFE1KM7:TR?= NIfXd24zOzFzNkG2PC=>
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NCI-H2170 M{LvTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTJwM{S3PFMh|ryP MkTSV2FPT0WU
EW-18 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXn6S3pUUUN3ME2yMlM2PDdzIN88US=> M4\GbnNCVkeHUh?=
KU812 NHnwSYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTkOWZyUUN3ME2yMlM3Pzd7IN88US=> NEDtV2FUSU6JRWK=
NY MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTJwM{eyO{DPxE1? NVS4UGxSW0GQR1XS
HTC-C3 M4fHN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfCTWM2OD1{LkSyNlY4KM7:TR?= M3fURXNCVkeHUh?=
CFPAC-1 NG\0dHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTJwNEO4OlQh|ryP NWfwXmU3W0GQR1XS
HH NUnZbVlwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHOzd4ZKSzVyPUKuOFk5PTRizszN MoXNV2FPT0WU
MKN7 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmD6TWM2OD1{Lk[yO|Q5KM7:TR?= MWDTRW5ITVJ?
TE-8 Mn3ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTJwNkO2OVMh|ryP Mnj6V2FPT0WU
MC-IXC MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXOTWM2OD1{Lk[0OFU{KM7:TR?= NVuxV|FwW0GQR1XS
DMS-79 M3;Pe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHUTpNKSzVyPUKuOlkzQDRizszN NIPWeZpUSU6JRWK=
Detroit562 Mm\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPlTWM2OD1{Lk[5PFQ2KM7:TR?= NFjpT5JUSU6JRWK=
HuP-T4 MkKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHjXFBTUUN3ME2yMlczQDF6IN88US=> M2jDbHNCVkeHUh?=
QIMR-WIL NYHSSosyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzkPFFGUUN3ME2yMlc2QTV{IN88US=> M3rmbnNCVkeHUh?=
BB65-RCC MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELDWmRKSzVyPUKuPFA4OTNizszN M2O5fXNCVkeHUh?=
PC-3 NInsXnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTJwOEOzNVch|ryP MUXTRW5ITVJ?
OVCAR-3 M1m0PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUK4Nlh5UUN3ME2yMlg2PTl2IN88US=> M4DZSXNCVkeHUh?=
KYSE-510 M2n6Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTJwOE[3PFYh|ryP MWfTRW5ITVJ?
HC-1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYX1WXFzUUN3ME2yMlk1ODF{IN88US=> NWPlZmM4W0GQR1XS
NCI-H1693 M4\IT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfDOo1wUUN3ME2yMlk2QDl5IN88US=> M3;UZXNCVkeHUh?=
DU-4475 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPjTWM2OD1{Lkm5NFUzKM7:TR?= MoK3V2FPT0WU
COLO-680N M3;CT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDZTWM2OD1|LkCwOFk2KM7:TR?= NXHvO4txW0GQR1XS
MN-60 NUPmNGRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTNwMES5O|Qh|ryP NVfoRYlDW0GQR1XS
BCPAP M120Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXLTWM2OD1|LkC4Nlk2KM7:TR?= NULUSVlDW0GQR1XS
647-V NFrQ[W5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPHTIVKSzVyPUOuNVYzOiEQvF2= MWjTRW5ITVJ?
HT-1376 M1PYPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TxWGlEPTB;Mz6yNFUxPiEQvF2= MoLWV2FPT0WU
NCI-H1793 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnyUGRKSzVyPUOuNlExQThizszN M2npdnNCVkeHUh?=
SW1463 NHr5N2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTCenY6UUN3ME2zMlI3OTl{IN88US=> MVXTRW5ITVJ?
NCI-H727 NGPObINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnOTWM2OD1|LkO4OVg1KM7:TR?= MWrTRW5ITVJ?
LB771-HNC M4PzNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{faT2lEPTB;Mz6zPVEzPiEQvF2= NHnqRZFUSU6JRWK=
D-542MG NYTjWHJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fVdmlEPTB;Mz60OFIzOiEQvF2= NHjDcpJUSU6JRWK=
NTERA-S-cl-D1 Mn\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTNwNES5OVch|ryP NH32flJUSU6JRWK=
C8166 NUGxeItCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\PNY1mUUN3ME2zMlQ3PTJzIN88US=> M1TLWHNCVkeHUh?=
639-V M1i5[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF[zSnVKSzVyPUOuOVc4QTJizszN M{DV[3NCVkeHUh?=
TE-1 MkPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\1d2lEPTB;Mz61PVE3QSEQvF2= MkPRV2FPT0WU
OAW-28 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTNwNUm0NlYh|ryP MWHTRW5ITVJ?
SK-LU-1 M4nZbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnL6TWM2OD1|Lk[wOlQ2KM7:TR?= M4nMfXNCVkeHUh?=
CaR-1 NYL2fZNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TLOWlEPTB;Mz62NVA1QCEQvF2= NV35To1xW0GQR1XS
A2780 M{jwfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHGZoNKSzVyPUOuOlQxQDlizszN MlS5V2FPT0WU
C2BBe1 M4TJdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTNwNkS0NFch|ryP MnPsV2FPT0WU
SK-MES-1 Mn61S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;FTWM2OD1|Lk[0OlE1KM7:TR?= MW\TRW5ITVJ?
PANC-10-05 MkfUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTNwN{KwO|Ih|ryP NF3JXWtUSU6JRWK=
J-RT3-T3-5 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTNwN{W0NFYh|ryP MoXQV2FPT0WU
ES4 MlX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\SUGVKSzVyPUOuPFIzQDZizszN MWHTRW5ITVJ?
NCI-H28 M2[0Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTNwOEK5OlEh|ryP MnHOV2FPT0WU
NCI-H1155 NHfmNJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTDTmlKSzVyPUOuPFQyPTlizszN NIrlcYpUSU6JRWK=
KURAMOCHI MnH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4m2bGlEPTB;Mz64OlA2PiEQvF2= NYDwXGlDW0GQR1XS
NCI-H69 MlvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTNwOUCxO|gh|ryP MknLV2FPT0WU
IST-MES1 M4HYcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXVTWM2OD1|Lkm0NFI6KM7:TR?= NUfUZpNpW0GQR1XS
NH-12 NF[4TVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTNwOUSxPFch|ryP NUDWZpN7W0GQR1XS
JVM-2 NVX4SFJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLxTWM2OD1|Lkm0O|UzKM7:TR?= NXzqd3ZkW0GQR1XS
EW-16 NEDOdYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHYN3VbUUN3ME2zMlk2PTd5IN88US=> MWjTRW5ITVJ?
NCI-H441 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTNwOUewNVQh|ryP NFrKeZZUSU6JRWK=
RCC10RGB NH\SSYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYq4blBMUUN3ME20MlAyOjR4IN88US=> MX;TRW5ITVJ?
TCCSUP M1TNZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\JU5NKSzVyPUSuNVExPSEQvF2= NED3eohUSU6JRWK=
HL-60 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDieWFKSzVyPUSuNVIzPDFizszN MnfRV2FPT0WU
EW-11 NWXXeI44T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLITWM2OD12LkGzNlYyKM7:TR?= M4C2bHNCVkeHUh?=
KARPAS-299 MkTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{e2NGlEPTB;ND6zNVAyOyEQvF2= NWPFdHVkW0GQR1XS
SNU-C2B M2jjUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTRwM{S2PFYh|ryP MknLV2FPT0WU
LU-139 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mmm1TWM2OD12LkO3NlQ4KM7:TR?= M1L3fXNCVkeHUh?=
HCC1937 MkD2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTRwM{e5PVMh|ryP NXzwRVlWW0GQR1XS
GR-ST NV7XfmU5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTRwNEOxPEDPxE1? MnrxV2FPT0WU
SK-OV-3 M2e1fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrLSFNKSzVyPUSuOFYxPDJizszN M1K1THNCVkeHUh?=
A172 NYP1OpZxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUG0d|R2UUN3ME20MlU2PTJzIN88US=> NEjhT3NUSU6JRWK=
NB10 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXWe2M6UUN3ME20MlU6PzhzIN88US=> M1fhTHNCVkeHUh?=
P30-OHK MkTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlG0TWM2OD12LkixPVI6KM7:TR?= NXi5TXMyW0GQR1XS
KYSE-520 NWfNU2U3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nWdGlEPTB;ND64PFAyQCEQvF2= MXjTRW5ITVJ?
KNS-62 NFvJPJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjafIVKSzVyPUSuPVA5ODdizszN MoT5V2FPT0WU
ES1 Moe1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3WyemlEPTB;ND65OlA{PSEQvF2= NXrROo5ZW0GQR1XS
KOSC-2 M2P6Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXKZm5yUUN3ME20Mlk6QTV7IN88US=> MXLTRW5ITVJ?
TE-9 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\SWopKSzVyPUWuNFE2OjVizszN MYfTRW5ITVJ?
NCI-H1838 NUfXOWV6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mly4TWM2OD13LkCyNFc3KM7:TR?= NIPvXI5USU6JRWK=
KP-N-YN MkiwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTVwMUK1PVEh|ryP M{TsRnNCVkeHUh?=
ALL-PO M4nWd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWK4c3FvUUN3ME21MlIzPTh5IN88US=> M17WXHNCVkeHUh?=
NOMO-1 M2TrWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTVwMkOxO|Yh|ryP NHm2UlVUSU6JRWK=
CGTH-W-1 Mo\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTVwM{i1PFQh|ryP M{LWWHNCVkeHUh?=
ATN-1 NYjhOpNmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7PTWM2OD13LkSxNVg1KM7:TR?= MVLTRW5ITVJ?
AGS MoPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTVwNEm2NVgh|ryP M{H2WnNCVkeHUh?=
SW13 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTVwNUOyOlUh|ryP NH:2fYJUSU6JRWK=
SW1990 M2nlSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{H3dWlEPTB;NT62NlI5PSEQvF2= M2T1OHNCVkeHUh?=
BFTC-905 NUKyN49PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTVwNkW4O|gh|ryP NUH2U5BOW0GQR1XS
RMG-I NXq3SplLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nKXmlEPTB;NT62OVk1OiEQvF2= NHizVYpUSU6JRWK=
HD-MY-Z MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjES|N1UUN3ME21MlY5PThzIN88US=> NFPJNnlUSU6JRWK=
HCC1187 Moi2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTVwN{GwN|Ih|ryP MYDTRW5ITVJ?
HCC2998 NXLwR3h5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLZUHJTUUN3ME21MlcyQDd5IN88US=> MVnTRW5ITVJ?
RT-112 NHTGeHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjFNGpYUUN3ME21MlkxOjR3IN88US=> Moe2V2FPT0WU
CAKI-1 NVXhWnk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXyOoU5UUN3ME22MlAxQTh3IN88US=> M4i5XnNCVkeHUh?=
HDLM-2 NGq0TolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTZwMEG0PFkh|ryP NXPSZZcyW0GQR1XS
TE-10 NUnoXZJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlO3TWM2OD14LkC0PFM{KM7:TR?= MXPTRW5ITVJ?
KE-37 NHHDb4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTZwMEeyOVgh|ryP NYDTTY5sW0GQR1XS
RCM-1 MnO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTZwMEe3NVkh|ryP M4Dzb3NCVkeHUh?=
Mewo NWCzU5VST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULMfHFzUUN3ME22MlEyPDh5IN88US=> MXfTRW5ITVJ?
L-428 NYrtcZIyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLNe5JKSzVyPU[uNVgxOzdizszN MYDTRW5ITVJ?
8-MG-BA M33TXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTZwMUmzO|kh|ryP NWnaUmZ1W0GQR1XS
SK-MEL-28 NGHzV3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\WZWRKSzVyPU[uNlA6PjdizszN NV64XlRrW0GQR1XS
VA-ES-BJ MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTZwM{C0OlIh|ryP NFTVTWpUSU6JRWK=
CAL-120 M{LEbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHFfmJIUUN3ME22MlQ2OTZ{IN88US=> NWjsXGxGW0GQR1XS
GAMG NIfqb4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGq3WplKSzVyPU[uOVA3OiEQvF2= MlflV2FPT0WU
MSTO-221H MlrKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF:5cotKSzVyPU[uOVEyPyEQvF2= MoXKV2FPT0WU
GCT Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTZwNUOzPFch|ryP NFXkZVNUSU6JRWK=
SH-4 MnK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTZwNUi4OFQh|ryP NWX1SZVvW0GQR1XS
Calu-3 NYS1[G84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTZwNkW1Olgh|ryP NGDnXXVUSU6JRWK=
SK-HEP-1 NEXjXZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHPTWM2OD14LkexO|U{KM7:TR?= NIPYcmVUSU6JRWK=
PFSK-1 M3\ofWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjqVVB7UUN3ME22Mlc{PDd6IN88US=> NWH6S5FNW0GQR1XS
NB12 Mly3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjZWIRKSzVyPU[uO|g1ODRizszN NIfNVnZUSU6JRWK=
A388 M3T0bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHNTWM2OD14Lki5OVIh|ryP MlTDV2FPT0WU
KLE NHfxXnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjFZnA{UUN3ME22Mlk3PTF4IN88US=> MYrTRW5ITVJ?
TE-11 Ml3RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlPHTWM2OD15LkC0PVY4KM7:TR?= MnvJV2FPT0WU
NCI-H2405 NVvW[WJTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoezTWM2OD15LkC4N|k{KM7:TR?= NYXJWm1RW0GQR1XS
OVCAR-4 NIHnPWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3v0O2lEPTB;Nz6xOVM2KM7:TR?= MkfOV2FPT0WU
KP-N-YS NIX4XJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofqTWM2OD15LkG4NVQh|ryP M3jHTXNCVkeHUh?=
NOS-1 MkPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnTN2ROUUN3ME23MlI2QDJ6IN88US=> NYHNO2dkW0GQR1XS
DMS-114 NILVOGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XLcWlEPTB;Nz6yPVY{OSEQvF2= NGfxRXBUSU6JRWK=
NCI-H2030 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnX2TWM2OD15LkOzOlk5KM7:TR?= M3nydnNCVkeHUh?=
HEL MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnxTpdKSzVyPUeuN|c2OjFizszN MnGxV2FPT0WU
CAL-62 M4TQbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;TfWlEPTB;Nz6zPVg3PiEQvF2= MlrWV2FPT0WU
COLO-668 M1jNTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjmO3ZKSzVyPUeuOFM6PjdizszN MWXTRW5ITVJ?
SKG-IIIa MlzVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXFeXVKSzVyPUeuOFU{QDFizszN M3fN[3NCVkeHUh?=
HCC1419 NWPlSoh7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTdwNE[4N|Mh|ryP NIDXUnNUSU6JRWK=
OS-RC-2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTdwNEm4PVQh|ryP NY\sVnFoW0GQR1XS
COLO-829 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTdwNk[0OlMh|ryP MoXKV2FPT0WU
NB13 M4PSfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPFTWM2OD15LkiyOlY4KM7:TR?= NX64UWYxW0GQR1XS
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D-423MG MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHub202UUN3ME2zPE41QDZ7IN88US=> Ml7lV2FPT0WU
NCI-H510A M2K1fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvHWm1KSzVyPUO4MlY1PTFizszN Mk\JV2FPT0WU
NMC-G1 M1TRUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHGTWM2OD1|OD63NlIzKM7:TR?= NWnyPZp1W0GQR1XS
SW948 MoO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfnTWM2OD1|OD64PVQ6KM7:TR?= M1;jSXNCVkeHUh?=
MFM-223 NHvpR4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjYVXlKSzVyPUO4Mlk1OzFizszN NEH1dJpUSU6JRWK=
PAN1 NFjWNo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7qSI1KSzVyPUO4Mlk6OTlizszN NVnYd4FwW0GQR1XS
MZ7-mel MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTN7LkGxPVkh|ryP NXq3TJE3W0GQR1XS
COLO-679 NXHvUnY6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrkOWVKSzVyPUO5Mlk4ODhizszN NX7USFlnW0GQR1XS
SW872 NFfZeGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXNTZA3UUN3ME20NE4zODNzIN88US=> MWXTRW5ITVJ?
HT-1197 M17wSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXSTIlKUUN3ME20NE4zQDZ7IN88US=> NHWwZZRUSU6JRWK=
ES6 NX3wUIhwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYD4UZRTUUN3ME20NE41PTV{IN88US=> M3nLRXNCVkeHUh?=
SNU-387 M2O3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLaZ5JKSzVyPUSwMlgzOzhizszN MkXxV2FPT0WU
T47D MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFL0b5pKSzVyPUSxMlAyQTJizszN MljuV2FPT0WU
G-402 MoPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TTbWlEPTB;NEGuNFgxQSEQvF2= MUPTRW5ITVJ?
MZ1-PC M4\4VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrRUFVWUUN3ME20NU45OSEQvF2= Mn\MV2FPT0WU
SW1417 MmPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MniyTWM2OD12Mj6zNFMh|ryP MlPiV2FPT0WU
RERF-LC-MS MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTR{LkWwPFch|ryP MnLzV2FPT0WU
SF539 NVX0WnBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TFNGlEPTB;NEKuOVUzQSEQvF2= M3;zT3NCVkeHUh?=
ESS-1 NHziZmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7QTWM2OD12Mz61OVU5KM7:TR?= M3u3N3NCVkeHUh?=
RO82-W-1 NF7CT|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XDbGlEPTB;NEOuOlE2OyEQvF2= M4LzSHNCVkeHUh?=
S-117 M{XCSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4S3V2lEPTB;NEOuOlk2QCEQvF2= M3uxVnNCVkeHUh?=
SW756 NUjWNlk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPGTWM2OD12Mz64NlQzKM7:TR?= NF\jOFRUSU6JRWK=
CP66-MEL MlrqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2r5TWlEPTB;NEWuO|E1OyEQvF2= NYjVWGhuW0GQR1XS
H-EMC-SS MnH3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTR3Lke2PVMh|ryP MYnTRW5ITVJ?
NCI-H2052 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnyZnE6UUN3ME20OU46ODJ5IN88US=> MXPTRW5ITVJ?
SW684 M1vLT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;VTWM2OD12Nz6wNFgyKM7:TR?= NInjUIVUSU6JRWK=
HCC1569 M{fGN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\XXlNKSzVyPUS3MlE3PThizszN MmjPV2FPT0WU
KGN Mle2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TRfmlEPTB;NEeuN|MyOSEQvF2= M3\pWHNCVkeHUh?=
LCLC-103H NXHYfYhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH2yOoVKSzVyPUS3MlQ1ODRizszN MV;TRW5ITVJ?
SW780 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnpc3VKSzVyPUS3MlY{PTFizszN NV\XeY9sW0GQR1XS
NCI-H1703 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLsVVZqUUN3ME20PE4{OzV{IN88US=> Mn7mV2FPT0WU
HCC1395 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnsbG5KSzVyPUS4Mlc1OzJizszN MknEV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03720873 Recruiting EGFR Gene Mutation Fujian Cancer Hospital October 2018 Phase 2
NCT03599518 Recruiting Non Small Cell Lung Cancer Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. October 10 2018 Phase 1
NCT03653546 Not yet recruiting Non-small Cell Lung Cancer|EGFR Gene Mutation|Brain Metastases Alpha Biopharma (Jiangsu) Co. Ltd. August 31 2018 Phase 2|Phase 3
NCT03602027 Not yet recruiting Non-squamous Non-small Cell Lung Cancer Second Affiliated Hospital of Nanchang University August 1 2018 Not Applicable
NCT03656393 Recruiting NSCLC|EGFR Shenzhen People''s Hospital August 31 2018 Phase 3
NCT03529084 Withdrawn Carcinoma Non-small Cell Lung Novartis Pharmaceuticals|Novartis July 23 2018 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID