Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 165 Publications

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Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M3HhWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVS2XYZnUUN3ME2wMlA2PCEQvF2= MoP2NVY2OTZ2N{O=
NIH3T3 MXLGeY5kfGmxbjDBd5NigQ>? MV:wMlIh|ryP NXXHboRzOiCq NXzFXHdZTE2VTx?= NWjKcpdSUW6qaXLpeIlwdiCxZjDFS2Yhe3SrbYXsZZRm\CCqdX3hckBmemKEMTDheZRweGixc4Doc5J6dGG2aX;uJJdqfGhiSVO1NEBw\iByLkCxOFQh|ryP MVGxOlQ5ODJ6NB?=
COS7 M4\XTmZ2dmO2aX;uJGF{e2G7 NV3OdZliQTZizszN MnLkTY5pcWKrdHnvckBw\iCqdX3hckBLSUt{IG[2NVdHKG23dHHueEBmgHC{ZYPz[YQ> MlPtNVcyPzh5MkK=
A549 NEf3ZnZEgXSxdH;4bYMhSXO|YYm= M4HPflk3KGh? MmjwSG1UVw>? MXfJR|UxRTF|LkW5JO69VQ>? MnXBNVg{OTN6MEe=
K562 NHqzZo9EgXSxdH;4bYMhSXO|YYm= NIDjSmI6PiCq NX\UW5dLTE2VTx?= M1O3VGlEPTB;OT6zOkDPxE1? MWixPFMyOzhyNx?=
MDA-MB-231 MXXDfZRwfG:6aXOgRZN{[Xl? NHvjUZQ6PiCq MkS0SG1UVw>? MnfETWM2OD1|Nj62NUDPxE1? NV\lSGxbOTh|MUO4NFc>
MOLT4 MX7DfZRwfG:6aXOgRZN{[Xl? NVzJWGhkQTZiaB?= M2C2OmROW09? NF72cWNKSzVyPUG1MlAzKM7:TR?= NFfz[3MyQDNzM{iwOy=>
MCF7 NH;ub3BEgXSxdH;4bYMhSXO|YYm= MoXTOVAh|ryP MYi3NkBp MlfrTWM2OD1zMj6wOUDPxE1? MmnMNVg4PzF6MUm=
BT474 M{DDS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV[3NkBp M1S3O2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS0O|Qh[2WubIOgc5ZmemW6cILld5NqdmdiRWLCZlIhf2m2aDDFR|UxKG:oIECuN{DPxE1? MXuxPVAzQDR{NB?=
HN5 MoGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHRNFE4OiCq M4DvZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO MnfTNVkxOjh2MkS=
BA/F3 Mnf3R5l1d3SxeHnjJGF{e2G7 NWn1fIR7PzJiaB?= NH25RZpEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBDSS:IMzDj[YxteyCneIDy[ZN{cW6pIFXHSnIhVDh3OGKgcZV1[W62IIfpeIghUUN3MDDv[kAxNjBzMEig{txO M1P3NVE6OjN7MkK5
NIH3T3 NFLpNoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLDXWg4OiCq MoXWS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> NY\pfY92OTl4NkWzO|c>
MCF7 MX MkmySpVv[3Srb36gRZN{[Xl? M2\ZPFMxKG2rbh?= NV73fYE5UW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4yKM7:TR?= NXG0UWFSOTl7M{K5OlA>
A431 NX\TeFU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;pNVAh|ryP NEXkZYM4OiCq NH23OGpIUTVyPUCuOVE1KM7:TR?= M4frd|E6QTZ7NE[1
A431 MXfLbY5ie2ViQYPzZZk> M3npSFAvOSEQvF2= MlnMNUBp MWrJcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u NFLRNGoyQTl4OUS2OS=>
A431 NYHQd29pU2mwYYPlJGF{e2G7 Mmr1NVAxKM7:TR?= M2POdFExKG2rbh?= M{DjXXBzd2yxbnfl[EBqdmirYnn0c5J6KGGldHn2bZR6KG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v MnjaNVk6Pjl2NkW=
Sf9 MnjKT4lv[XOnIFHzd4F6 M1zOO|ExKG2rbh?= NYrq[oZRTE2VTx?= M1jjXWlvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= MkmzNlAxPTZ2MkW=
NCI-H1975 NF6wdG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TpSFUh|ryP NV3JSGE5PzJiaB?= NFHuV4NKSzVyPUiuNlYh|ryP M1[4eFIxOTVzNkew
NCI-H1975 MXTLbY5ie2ViQYPzZZk> MlnyNVAh|ryP NEXvfGdKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? Mni5NlAyPTF4N{C=
NCI-H1975 M33RS2Z2dmO2aX;uJGF{e2G7 NWLHeYRiOTBizszN NXjWT4xUUW6qaXLpeIlwdiCxZjDFVmJDOiCjdYTvdIhwe3Cqb4L5cIF1cW:w NXXpflc1OjBzNUG2O|A>
NCI-H1975 M3nnWGZ2dmO2aX;uJGF{e2G7 NYnycpRCPSEQvF2= NGPI[pg1QCCq NYrGW2R4UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHPhd5Bie2VvMx?= MXWyNFE2OTZ5MB?=
A375 M3foPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\tdZE1QCCq M4LUSmlEPTB;MkCuOVUh|ryP M1jiXFIxOzB2NUO3
SMMC7721 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvzOFghcA>? MkLHTWM2OD1|Mz6xNkDPxE1? M3vYUVIxOzB2NUO3
DU145 NWnFfHdvS3m2b4TvfIlkKEG|c3H5 NEDX[242KGR? MWLEUXNQ Ml;mTWM2OD12Mj6xO{DPxE1? M3LPOlIxPDZ4NUW1
MIAPaCa2 M2rp[mN6fG:2b4jpZ{BCe3OjeR?= MWW1JIQ> NHjqWYdFVVOR MUnJR|UxRTVyLk[yJO69VQ>? MlHsNlA1PjZ3NUW=
PANC1 MWjDfZRwfG:6aXOgRZN{[Xl? NUXvOYxpPSCm M3LBWGROW09? MX;JR|UxRTRyLkSyJO69VQ>? NW\sSI16OjB2Nk[1OVU>
PC9 Mnj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfxeY5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN NVfBbXE2OjF{MEi4NFI>
PC9 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjJc5pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIdzd3e2aD3y[ZNqe3SjboSgbJVu[W5iUFO5JINmdGy|IHX4dJJme3OrbnegSWdHWiCHN{S2Y2E4PTBxVEe5NG0hdXW2YX70JJdqfGhiRVO1NEBw\iB5IN88US=> MUCyNVIxQDhyMh?=
PC3 M1HRUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXOxNEDPxE1? M373flczKGh? M2\2WGROW09? M{HCVWlEPTB;Nz60JO69VQ>? MnXTNlE{PTN3NE[=
MDA-MB-468 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PsfVExOCEQvF2= MXO0PEBp MXXEUXNQ NFfDZYRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> NYHGUJFxOjF2Mkm2N|I>
Plasmodium falciparum MlrTdWhVWyCycn;mbYxqdmdiZn;yJIlvcGmkaYTvdpMhd2ZiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJJBzd2yrZnXyZZRqd25? NW[3dlNpOjF6MUewOFU>
NCI-H1993 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFv3Toc4OiCq M2TxXGROW09? NH7sVnlKSzVyPUCuNUDPxE1? NHLoWG8zOTlzNkSzNy=>
NCI-H292 MoG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjCPFY4OiCq MlPnSG1UVw>? M3LvWGlEPTB;MD6wNkDPxE1? MkDqNlE6OTZ2M{O=
A431 MYTLbY5ie2ViQYPzZZk> NEjLcmgzKGh? NFjvOZNFVVOR M4jZVmlvcGmkaYTpc44hd2ZiRVfGVkBxcG:|cHjvdplt[XSrb36gbY4hTUeILYP0bY12dGG2ZXSgbJVu[W5iQUSzNUB4cXSqIFnDOVAhd2ZiMD6wNFEh|ryP NEL4WYQzOjFyMUGzNi=>
MCF7 M1LTe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHsNVAxyqEQvHevcWw> MnnVOFghcA>? M{DCdGROW09? NGG0bpRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> NUHYeHpqOjJzMUmxN|A>
BT474 MoTMR5l1d3SxeHnjJGF{e2G7 MVuxNEDPxE1? Mki1OFghcA>? NILoNGVEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIFLUOFc1KGOnbHzz MoDZNlIyOzh|MEi=
MCF7 Ml7FR5l1d3SxeHnjJGF{e2G7 NEHJe|QyOCEQvF2= MX:0PEBp NF;pUW9EgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz MYCyNlE{QDNyOB?=
HCC827 M3vsWWtqdmG|ZTDBd5NigQ>? M17w[VAvOTJ3IN88US=> NGW4eIMzPCCq M4Lv[GROW09? M3zDUmlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v MV[yNlM{QTN2Mh?=
HCC827 NHXQNlVMcW6jc3WgRZN{[Xl? MmPENE4yOjVizszN MUeyOEBp Ml[4SG1UVw>? M4Wy[GlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCHcnugdIhwe3Cqb4L5cIF1cW:w NETpfmgzOjN|OUO0Ni=>
HCC827 MUDLbY5ie2ViQYPzZZk> M3XCVVAvOTJ3IN88US=> MkfnNlQhcA>? M3GxNWROW09? M3;VU2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w NYfrTHF[OjJ|M{mzOFI>
A431 NVT1dVFsU2mwYYPlJGF{e2G7 MUixNFAh|ryP NH3neVQ{OCCvaX6= NF3sWJdKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN MlHnNlI3QTh5OEK=
H460 NYLZ[pBIS3m2b4TvfIlkKEG|c3H5 NYWzdHlVPzJiaB?= Ml64TWM2OD13LkW5JO69VQ>? NGDvNnozOjd{N{S0PS=>
HT-29 M3rjVWN6fG:2b4jpZ{BCe3OjeR?= NGrHU2w4OiCq MV3JR|UxRTNwM{[g{txO MnzxNlI4Ojd2NEm=
SGC7901 NH\a[YZEgXSxdH;4bYMhSXO|YYm= NYfndHRnPzJiaB?= M3;NfWlEPTB;MUCuNlYh|ryP M1LYbVIzPzJ5NES5
A498 NYrnO4NxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2Ls[2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUS5PEBk\WyuczDlfJBz\XO|aX7nJGVITlJiYX7kJHNTSyC5aYToJGlEPTBib3[gPE43KM7:TR?= NFzFPXozOjhzOEi0PC=>
Calu3 M13WfGN6fG:2b4jpZ{BCe3OjeR?= M4XqdlExKM7:Zz;tUC=> M{foc|czKGh? MknCTWM2OD1zLk[g{txO MmnWNlMyOTZzNki=
FADU MXTDfZRwfG:6aXOgRZN{[Xl? M{DIOlExKM7:Zz;tUC=> NEDlXmM4OiCq M1LqXGlEPTB;MD6wOUDPxE1? NE[0elQzOzFzNkG2PC=>
HCC827 NXH2fnVyS3m2b4TvfIlkKEG|c3H5 MUixNEDPxGdxbVy= M3;rbVczKGh? MmXaTWM2OD1yLkCwNVUh|ryP NVPZcnRPOjNzMU[xOlg>
PC9 NVTEfHFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPQOWd{OTBizsznM41N MmHRO|IhcA>? NX:1XXVNUUN3ME2wMlAyPCEQvF2= NGfxXmgzOzFzNkG2PC=>
Sf9 NUX2fJN7U2mwYYPlJGF{e2G7 MUKxJIg> Mn\1SG1UVw>? M3v1eWlvcGmkaYTpc44hd2ZiaIXtZY4hT1OWLYTh[4dm\CCHR1\SJGw5OzSUIH31eIFvfCCneIDy[ZN{\WRid3n0bEBKSzVyIH;mJFAvODF3IN88US=> MmjUNlM3OTF4OUG=
16HBE14o- NUL4cZo{S3m2b4TvfIlkKEG|c3H5 M1ziZlExKM7:TR?= MY[3NkBp NHzwSWZFVVOR Mm\sR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gNVZJSkVzNH:tJINmdGy|IHjhdoJwemmwZzD3bYxlKHS7cHWgSWdHWiCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjD3bZRpKEmFNUCgc4YhOTJwN{Gg{txO NFuwfJYzOzZ4OES0NS=>
NCI-H1975 M1HNRmN6fG:2b4jpZ{BCe3OjeR?= NE[xUW0yOCEQvF2= NFPhSHk4OiCq NXv5RWJDTE2VTx?= NYrjd5RMS3m2b4TvfIlkcXS7IHHnZYlve3RiZ3XmbZRqdmmkLYLld4l{fGGwdDDoeY1idiCQQ1mtTFE6PzViY3XscJMhcGG{Yn;ybY5oKEWJRmKgUFg2QFJxVEe5NG0h\G:3YnzlJI12fGGwdDDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckB4cXSqIFnDOVAhd2ZiNj65NVch|ryP M{\JVlI{PjZ6NESx
16HBE MmjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXGzSZhEPzJiaB?= NEnnZ|ZFVVOR NUfsNmh4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjCxOmhDTSClZXzsd{BmgHC{ZYPzbY5oKHerbHSgeJlx\SCHR1\SJJdqfGhiSVO1NEBw\iBzLk[wNUDPxE1? NGLYNGYzOzd7MkOxPC=>
16HBE M2TFTmZ2dmO2aX;uJGF{e2G7 M3vyTlExOCEQvF2= M37DNFE2OCCvaX6= M4PWbWROW09? M1exdWlv\HWldHnvckBw\iCwaYTybYMhd3irZHWgdJJw\HWldHnvckBqdiCqdX3hckAyPkiERTDj[YxteyCneIDy[ZN{cW6pIIfpcIQhfHmyZTDFS2ZT MmTCNlM4QTJ|MUi=
A549 NVznfIhqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofsO|IhcA>? NEixNGdFVVOR MmnDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDD3bYxlKHS7cHWgSWdHWi:tLWLhd{Bl\XCnbnTlcpQhcHWvYX6gRVU1QSClZXzsd{B4cXSqIFnDOVAhd2ZiND62NlYh|ryP M3PNW|I{Pzl{M{G4
SH-SY5Y MXLDfZRwfG:6aXOgRZN{[Xl? MYm3NkBp M4n5b2lEPTB;MUiuNlEh|ryP NULTfGdFOjN6N{G5NFk>
LoVo MlXnT4lv[XOnIFHzd4F6 M1j3WFIhKGh? NHLSS2xFVVOR M2nQOmlvcGmkaYTpc44hd2ZiRVfGVkBxcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwME[yJO69VQ>? M4CxRVI{QTNyOUm0
PC9 NV3wVWo{U2mwYYPlJGF{e2G7 NXG2TXh[OiCq NXnWO5ptTE2VTx?= M3PVbmlvcGmkaYTpc44hd2ZiRVfGVkBmgG:wIEG5JIRmdGW2aX;uJIFkfGm4YYTpcochdXW2YX70JJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDQR|kh[2WubIOge4l1cCCLQ{WwJI9nKDBwMEC4O{DPxE1? NGezV2QzOzl|MEm5OC=>
HCC827 NID5fFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXq3NkBp NVj5PVE1UUN3ME2wMlAyOiEQvF2= NWLKd5NjOjN7N{OxOlg>
A549 MXvLbY5ie2ViQYPzZZk> NFPwO4EyKM7:TR?= NG\UcZMyKGh? NYLZbHRnTE2VTx?= NFfzd2xKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzl7IN88US=> M4DrbVI{QTh6M{W0
A549 NYPQbmxzTnWwY4Tpc44hSXO|YYm= Mom4NUDPxE1? MYmxJIg> MnvCSG1UVw>? Mn;ZR4VtdHWuYYKgeZB1[Wun MWSyN|k5QDN3NB?=
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HCC827 MVzGeY5kfGmxbjDBd5NigQ>? NVHiXoFFOC53IN88US=> NUjLfldHOiCq NYTNTod{TE2VTx?= NFXWeppKdmirYnn0bY9vKG:oIHOtUYV1KHCqb4PwbI9zgWyjdHnvckBqdiCpZX\peIlvcWJvcnXzbZN1[W62IHj1cYFvKEiFQ{iyO{Bk\Wyucx?= NHHOSYEzOzl7M{OyPC=>
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TK10 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLEOJZuUUN3ME21NVUvOTFibl2= MmnEV2FPT0WU
HN NYj5VmlUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTVzNz6zPEBvVQ>? MXjTRW5ITVJ?
SCC-15 NEjCNWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPySJBKSzVyPUWyOU43QCCwTR?= MWnTRW5ITVJ?
NCI-H358 M3izU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlr2TWM2OD13MkeuOlYhdk1? M36yOHNCVkeHUh?=
KYSE-180 Mn35S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4L2TmlEPTB;NUOwMlYzKG6P M1;QTXNCVkeHUh?=
NCI-H82 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTV2MD61JI5O NFHuVoVUSU6JRWK=
NB7 NX3NN5ZYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTV2Nj6zNkBvVQ>? NFrNTYZUSU6JRWK=
DSH1 MmH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTV4Nj64PEBvVQ>? MnrlV2FPT0WU
RS4-11 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV33dmgzUUN3ME21OlgvPTlibl2= MXrTRW5ITVJ?
CAN NH;JPHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjCPIFTUUN3ME21PFAvPzZibl2= MmDiV2FPT0WU
Ca9-22 NIXyNo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjwPYFQUUN3ME21PFgvPjRibl2= M3HyOnNCVkeHUh?=
CAL-39 M4S3Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoW1TWM2OD13OU[uN{BvVQ>? M2foXnNCVkeHUh?=
NCI-H1623 M1LBUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTV7OT6zOkBvVQ>? NXm5XXNrW0GQR1XS
FADU M3f3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTZyND62PUBvVQ>? NGfsUWpUSU6JRWK=
NB69 Ml3BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2f2UGlEPTB;NkG0MlM2KG6P MWfTRW5ITVJ?
Ramos-2G6-4C10 NGHhfVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3sTWM2OD14MkGuOFYhdk1? NXXFd4xLW0GQR1XS
LB1047-RCC NH\XbolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHhbHl[UUN3ME22NlUvOjlibl2= MmnCV2FPT0WU
MV-4-11 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\nTWM2OD14MkeuO{BvVQ>? M{TLTXNCVkeHUh?=
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ME-180 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPIWXJKSzVyPU[zNk46KG6P MVfTRW5ITVJ?
NBsusSR M4LKdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHSTWM2OD14NE[uOVIhdk1? MmPPV2FPT0WU
COR-L105 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3JXHJyUUN3ME22OlIvPTNibl2= NGPzSVRUSU6JRWK=
ETK-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\WTmlEPTB;Nk[yMlU6KG6P MnfQV2FPT0WU
L-363 NILRNY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXwNHpKSzVyPU[3NU46PCCwTR?= M{DHUHNCVkeHUh?=
LU-134-A NETLPHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPBNFVKSzVyPUexPU4xQSCwTR?= M4Lqe3NCVkeHUh?=
NCI-H1770 M1e1TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXzeXJ[UUN3ME23NlIvPCCwTR?= NF7tVJFUSU6JRWK=
HAL-01 MkXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfOTWM2OD15M{SuO|Ehdk1? NIG3fVlUSU6JRWK=
COLO-684 M3Lkd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknMTWM2OD15NUOuNFEhdk1? MUjTRW5ITVJ?
TE-6 NFLObJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjJcpNKSzVyPUe2OE4{OSCwTR?= M4PBXHNCVkeHUh?=
A431 MkTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\R[HdKSzVyPUe4NE4xPCCwTR?= NHPYVXpUSU6JRWK=
LU-135 NH;z[3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\BNWlEPTB;N{izMlA{KG6P NX3N[nlJW0GQR1XS
EC-GI-10 M4fTUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHwOHNKSzVyPUe4PE42OiCwTR?= M4jBbnNCVkeHUh?=
TE-12 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3q3O2lEPTB;OEKxMlEyKG6P NIXWfpVUSU6JRWK=
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23132-87 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\6RWlEPTB;OE[0MlI5KG6P MWPTRW5ITVJ?
AU565 MnXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLNbZd3UUN3ME24OlkvPiCwTR?= NXXaU5FyW0GQR1XS
DEL M37sRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLWTFdYUUN3ME24O|cvPjVibl2= MYrTRW5ITVJ?
COR-L88 M37LeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3nZTmlEPTB;OEiyMlI3KG6P NEXOWW5USU6JRWK=
NCI-H2126 NH;Oc|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLyTWM2OD16OEKuPFMhdk1? NFL0XoRUSU6JRWK=
CAPAN-1 NHi1U3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTK[5Q4UUN3ME24PFMvOjlibl2= NWDNPYFkW0GQR1XS
BT-474 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33iWGlEPTB;OUO4MlE1KG6P NYrneFNyW0GQR1XS
BHY NXe3T2hzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPaV4d2UUN3ME25O|UvPDhibl2= MYrTRW5ITVJ?
HT-3 NHTCeoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPCTWM2OD17OEKuOFEhdk1? MWjTRW5ITVJ?
SK-NEP-1 M1zwVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXlT|VDUUN3ME25PFkvPDRibl2= MkDIV2FPT0WU
MS-1 M1G1Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\PNVJKSzVyPUm5OE43QSCwTR?= Ml\yV2FPT0WU
DOHH-2 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDFU3pjUUN3ME2xMlAxODJzIN88US=> NYDvZm1LW0GQR1XS
NCI-H1304 NH7KRWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvTcXFKSzVyPUGuNFE3PDNizszN NVXUSGx{W0GQR1XS
EM-2 MnrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIS3SGlKSzVyPUGuNFMxOTZizszN MUfTRW5ITVJ?
MHH-PREB-1 NITjOZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4f3dGlEPTB;MT6wPFQ2OSEQvF2= MnfQV2FPT0WU
KU-19-19 M{W4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHtS29YUUN3ME2xMlA5PTRzIN88US=> Mn7mV2FPT0WU
NCI-H1648 NEXGe2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rWZ2lEPTB;MT6xNFYyPiEQvF2= NFvtSmtUSU6JRWK=
MKN1 NW\DWo5UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTFwMkG5PFch|ryP NF;RbWxUSU6JRWK=
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GCIY M3G4bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjMeYxKSzVyPUGuN|AyOzJizszN M2ixUHNCVkeHUh?=
CTV-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPldllKSzVyPUGuN|A5KM7:TR?= NHzIN2VUSU6JRWK=
NCI-H1092 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTFwM{C4OUDPxE1? MnT6V2FPT0WU
NKM-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLUTGY6UUN3ME2xMlMzPTl5IN88US=> NH7vdFBUSU6JRWK=
NCI-H1650 MoXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;OTWM2OD1zLkOzOFgzKM7:TR?= MoG5V2FPT0WU
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SW48 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTFwM{[2NVgh|ryP NIXwT4FUSU6JRWK=
SK-N-DZ MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHNV2pKSzVyPUGuN|Y6PDdizszN NIDYbopUSU6JRWK=
KMOE-2 NUDHfIdjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vvUmlEPTB;MT6zPVgyPSEQvF2= M3X0WnNCVkeHUh?=
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CCRF-CEM MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoP0TWM2OD1zLkSxNVEh|ryP NFrTUZdUSU6JRWK=
RH-1 M4TuSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLqTWM2OD1zLkSxNVM3KM7:TR?= MULTRW5ITVJ?
NCI-H526 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrZSG1KSzVyPUGuOFM5OjZizszN MXHTRW5ITVJ?
697 Mm[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfjTWM2OD1zLkS0NVA1KM7:TR?= MkXpV2FPT0WU
J82 NI\RS45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPMTWM2OD1zLkS1O|Y3KM7:TR?= M2fzW3NCVkeHUh?=
BE-13 Ml3jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXU[GU1UUN3ME2xMlUxPjdzIN88US=> M4G5cnNCVkeHUh?=
SAS NH7tSWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzWTWM2OD1zLkWxOlIzKM7:TR?= MoThV2FPT0WU
SCC-25 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTFwNUKzNlIh|ryP NYq2ZphLW0GQR1XS
LAMA-84 NFHpe4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfFfW16UUN3ME2xMlU{QDF3IN88US=> M{HJcHNCVkeHUh?=
HSC-3 NHvkb5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;jZlliUUN3ME2xMlU1OzhizszN NHuxSWNUSU6JRWK=
BT-549 MkXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjpNJdKSzVyPUGuOVg4PDdizszN NGG2doFUSU6JRWK=
ML-2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnHbpZLUUN3ME2xMlY1ODJ7IN88US=> NVLRZopSW0GQR1XS
BB30-HNC M3TjcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jVcWlEPTB;MT62PFk4OiEQvF2= Mm\WV2FPT0WU
PANC-03-27 NIHpbIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTFwN{CxN|kh|ryP MVXTRW5ITVJ?
COLO-678 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXqcFNKSzVyPUGuO|M1PDRizszN MmrGV2FPT0WU
NCI-H1666 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPVUXpKSzVyPUGuO|Q4ODRizszN NH3VRYNUSU6JRWK=
NCI-H209 M2P5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTFwN{eyOlkh|ryP MWrTRW5ITVJ?
EPLC-272H MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjiZWRKSzVyPUGuPFU2PDlizszN M4PT[3NCVkeHUh?=
OMC-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTFwOUm4PFgh|ryP NFPiclZUSU6JRWK=
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CAL-12T MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fMVmlEPTB;Mj6wNVg3OSEQvF2= NWP2cnBQW0GQR1XS
TGBC24TKB M4TVb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTJwMEK5OlEh|ryP MUPTRW5ITVJ?
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NCI-H2009 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLsbWZnUUN3ME2yMlA3OjF2IN88US=> NFPPOlhUSU6JRWK=
RPMI-8866 NVL2ZVlnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTJwMEi0PFEh|ryP NETjbHBUSU6JRWK=
KARPAS-45 NFvHRXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTJwMUK0O|kh|ryP M1WyW3NCVkeHUh?=
RXF393 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTJwMUOwN|Yh|ryP NXXXZpVtW0GQR1XS
786-0 Mkj6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXveG1KSzVyPUKuNVg1ODZizszN MXPTRW5ITVJ?
SCC-4 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTJwMkG3O|Yh|ryP NHPvXoZUSU6JRWK=
BT-20 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDYUWRSUUN3ME2yMlI3PzR2IN88US=> MWfTRW5ITVJ?
A498 M{XmW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LQSGlEPTB;Mj6yPVQyPSEQvF2= MmPDV2FPT0WU
IGROV-1 Ml\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYCzT5RFUUN3ME2yMlMyOjN5IN88US=> Mlj2V2FPT0WU
JVM-3 NXPIXmpzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLocI5zUUN3ME2yMlM1PjR|IN88US=> M1PHXXNCVkeHUh?=
NCI-H2170 MkHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4X2WmlEPTB;Mj6zOFc5OyEQvF2= M1OwcHNCVkeHUh?=
EW-18 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjuVlJEUUN3ME2yMlM2PDdzIN88US=> NIP4Z2tUSU6JRWK=
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HH M4fqZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTJwNEm4OVQh|ryP MYjTRW5ITVJ?
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MC-IXC NV7sWXp{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3kcpN[UUN3ME2yMlY1PDV|IN88US=> M1fDfHNCVkeHUh?=
DMS-79 M1zz[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTJwNkmyPFQh|ryP MXnTRW5ITVJ?
Detroit562 Mn\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXWd5RKSzVyPUKuOlk5PDVizszN M4f4TnNCVkeHUh?=
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QIMR-WIL M3TjVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHzZ3lKSzVyPUKuO|U6PTJizszN MVjTRW5ITVJ?
BB65-RCC NEOzXGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTJwOEC3NVMh|ryP NGKxbnBUSU6JRWK=
PC-3 M4X6dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELzVoNKSzVyPUKuPFM{OTdizszN NXfUUYZ7W0GQR1XS
OVCAR-3 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHqXYFKSzVyPUKuPFU2QTRizszN NFLnb25USU6JRWK=
KYSE-510 M3vUVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTkSJRqUUN3ME2yMlg3Pzh4IN88US=> MnTGV2FPT0WU
HC-1 M{XicWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHVWY5KSzVyPUKuPVQxOTJizszN NE\XVXhUSU6JRWK=
NCI-H1693 MojoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nyN2lEPTB;Mj65OVg6PyEQvF2= NFz2VG5USU6JRWK=
DU-4475 NF\t[HRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF2zbGZKSzVyPUKuPVkxPTJizszN MlHDV2FPT0WU
COLO-680N MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTaTWM2OD1|LkCwOFk2KM7:TR?= MWjTRW5ITVJ?
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NCI-H1838 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTVwMEKwO|Yh|ryP MVnTRW5ITVJ?
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RT-112 NU\qVYRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXQTXNuUUN3ME21MlkxOjR3IN88US=> M1raSHNCVkeHUh?=
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GCT NFfYXHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmG1TWM2OD14LkWzN|g4KM7:TR?= NVzaXJoxW0GQR1XS
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PFSK-1 MkC4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPLTWM2OD14LkezOFc5KM7:TR?= MUnTRW5ITVJ?
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A388 Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\hTWM2OD14Lki5OVIh|ryP NUi4bZpLW0GQR1XS
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NCI-H2405 NG\a[lFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jvR2lEPTB;Nz6wPFM6OyEQvF2= MV7TRW5ITVJ?
OVCAR-4 NF7YTnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\udotKSzVyPUeuNVU{PSEQvF2= MULTRW5ITVJ?
KP-N-YS NEjNWXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\WTW5KUUN3ME23MlE5OTRizszN MVHTRW5ITVJ?
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DMS-114 MlznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTdwMkm2N|Eh|ryP Ml3KV2FPT0WU
NCI-H2030 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXUTWM2OD15LkOzOlk5KM7:TR?= MkfsV2FPT0WU
HEL M4\wfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXaTWM2OD15LkO3OVIyKM7:TR?= NWe4NFJYW0GQR1XS
CAL-62 NF\Oe4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjXT5hrUUN3ME23MlM6QDZ4IN88US=> NEP1SYNUSU6JRWK=
COLO-668 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrITWM2OD15LkSzPVY4KM7:TR?= NVXyVmtwW0GQR1XS
SKG-IIIa NHrGc4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPwZnR7UUN3ME23MlQ2OzhzIN88US=> NVLQ[mtZW0GQR1XS
HCC1419 NXfUeGd[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLRTWM2OD15LkS2PFM{KM7:TR?= NYH5bZQ2W0GQR1XS
OS-RC-2 M3fkcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrqSGFnUUN3ME23MlQ6QDl2IN88US=> MmrMV2FPT0WU
COLO-829 MonyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTdwNk[0OlMh|ryP M37vc3NCVkeHUh?=
NB13 MoPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoD2TWM2OD15LkiyOlY4KM7:TR?= NIL1PG1USU6JRWK=
DK-MG MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjmVoNrUUN3ME23Mlg{OjBzIN88US=> Mn\5V2FPT0WU
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NCI-H661 MlfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTlbG9KSzVyPUiuNFU5PjdizszN MYHTRW5ITVJ?
AM-38 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1O0bmlEPTB;OD6xOlI5KM7:TR?= NWDqepM{W0GQR1XS
VM-CUB-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4G3WWlEPTB;OD6xO|AzQSEQvF2= MYrTRW5ITVJ?
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EGI-1 MlWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRThwNkCwPFgh|ryP MVzTRW5ITVJ?
D-392MG M4\WdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRThwNk[yNVQh|ryP NELsW|JUSU6JRWK=
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NCI-H1792 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLkTWM2OD16Lki4PVA{KM7:TR?= NX64WplPW0GQR1XS
LS-1034 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjZRZNRUUN3ME24Mlk1PjV{IN88US=> NX7OVZpMW0GQR1XS
D-566MG MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTQTWM2OD17LkCxPFEh|ryP M1zNfHNCVkeHUh?=
NCI-H226 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrLTWM2OD17LkC5JO69VQ>? NXficG1NW0GQR1XS
ChaGo-K-1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDQS5A5UUN3ME25MlExPTd7IN88US=> MYTTRW5ITVJ?
NCI-H2228 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nOeWlEPTB;OT6xNVA5OSEQvF2= MoiyV2FPT0WU
RPMI-8226 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfRfog4UUN3ME25MlEyOzB2IN88US=> MV7TRW5ITVJ?
BFTC-909 NF;K[mlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HmNGlEPTB;OT6xPFI6PyEQvF2= MVfTRW5ITVJ?
HPAF-II MlvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHjRlJKSzVyPUmuNlI2OThizszN NFnDd3dUSU6JRWK=
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IA-LM NVL5eWJzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LPZWlEPTB;OT60NVk3PiEQvF2= NILsbHhUSU6JRWK=
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NCI-H2052 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLVTWM2OD12NT65NFI4KM7:TR?= M4HDN3NCVkeHUh?=
SW684 NG\oN5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTR5LkCwPFEh|ryP MYnTRW5ITVJ?
HCC1569 MnvPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXvTWM2OD12Nz6xOlU5KM7:TR?= NXnTd|kyW0GQR1XS
KGN NU\uR|Q4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnBS21KSzVyPUS3MlM{OTFizszN NUfycHJjW0GQR1XS
LCLC-103H MkXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTR5LkS0NFQh|ryP M1TpUHNCVkeHUh?=
SW780 NHTOUmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITmWo5KSzVyPUS3MlY{PTFizszN NID3d2hUSU6JRWK=
NCI-H1703 NGnCOnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTR6LkOzOVIh|ryP NVXROpNrW0GQR1XS
HCC1395 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7GZZlSUUN3ME20PE44PDN{IN88US=> MYfTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
phospho-EGFR / phospho-ERK / phospho-SRC / phospho-AKT; 

PubMed: 22586683     


Effect of overexpressing CRKL on phosphorylation levels of EGFR, ERK, AKT and SRC in response to gefitinib. HCC827 cells expressing a control vector or CRKL were exposed to gefitinib at indicated concentrations for 6 h and cell lysates were collected for immunoblotting with indicated specific antibodies.

Cyclin D1/ p16 / p27 / pRb; 

PubMed: 18559346     


levels of phosphorylated and total EGFR, IGF-1R, Akt, ERK1/2, and Rb and levels of cyclin D1, p27, p16, and actin following 6- or 16-h treatment of A431 cells with combinations of 1 or 3 μm gefitinib and 3 μm NVP-ADW742. Data are representative of two independent experiments.

uc-caspase-9 / c-caspase-9 / uc-caspase-3 / c-caspase-3 / uc-PARP / PARP; 

PubMed: 23102728     


Apoptosis resulting from combined treatment with gefitinib and an MEK inhibitor. (A) apoptosis relating protein induced by gefitinib and/or AZD6244 in PC‐9/wt and PC‐9/gef B4 cells. (B) Apoptosis relating protein induced by gefitinib and/or CI‐1040 in PC‐9/wt and PC‐9/gef B4 cells. (C) apoptosis relating protein induced by gefitinib and/or AZD6244 in PC‐9/gefE3 and PC‐9/gefE7 cells. (D) apoptosis relating protein induced by gefitinib and/or CI‐1040 in PC‐9/gefE3 and PC‐9/gefE7 cells.

PPAR-γ; 

PubMed: 23409107     


Fold increase relative to control was determined after normalization with β-actin as external loading control. 

22586683 18559346 23102728 23409107
Immunofluorescence
EGFR / LC3; 

PubMed: 31196210     


H157 and H1299 cells were treated with gefitinib, or curcumin alone, or gefitinib plus curcumin at indicated concentration for 48 h and then were fixed with methanol, immunostained with anti-EGFR (green), anti-LC3 (red), and DAPI (blue), and analyzed by confocal microscopy to determine the intracellular locations of EGFR and LC3.

PPAR-γ; 

PubMed: 23409107     


Upregulation and nuclear accumulation of PPARγ following treatment with gefitinib (24 hs).

p-EGFR / β-catenin; 

PubMed: 23461856     


In untreated CNE2 cells, β-catenin was located in cytoplasmic membranes and cytoplasm. Treatment with EGF (50 ng/mL) resulted in increased β-catenin nuclear staining, which was reversed by either gefitinib (1 μM) or LY294002 (15 μM). 

31196210 23409107 23461856
Growth inhibition assay
GI50; 

PubMed: 23409107     


Dose-response of bladder cancer cell lines to EGFR inhibitor (gefitinib). The GI50 value was defined as the mean concentration of drug that generates 50% of growth inhibition as compared to controls.

23409107
In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03866499 Not yet recruiting NSCLC Beta Pharma Inc. May 2019 Phase 3
NCT03866499 Not yet recruiting NSCLC Beta Pharma Inc. May 2019 Phase 3
NCT03787992 Not yet recruiting Locally Advanced or Metastatic EGFR Sensitising Mutation Positive Non-small Cell Lung Cancer Allist Pharmaceuticals Inc. April 1 2019 Phase 3
NCT03787992 Not yet recruiting Locally Advanced or Metastatic EGFR Sensitising Mutation Positive Non-small Cell Lung Cancer Allist Pharmaceuticals Inc. April 1 2019 Phase 3
NCT03766490 Not yet recruiting Non-Small-Cell Lung The First Affiliated Hospital of Soochow University|The First People''s Hospital of Changzhou|Second Affiliated Hospital of Soochow University|Nantong University|Affiliated Hospital of Jiangnan University|Jiangyin People''s Hospital|Changzhou No.2 People''s Hospital|Chia Tai Tianqing Pharmaceutical Group Co. Ltd. March 30 2019 Not Applicable
NCT03856697 Not yet recruiting Advanced Non-small Cell Lung Cancer Hangzhou ACEA Pharmaceutical Research Co. Ltd.|Guangdong Provincial People''s Hospital March 2019 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID