Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 95 Publications

14 Customer Reviews

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NEe1OmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\BZog6UUN3ME2wMlA2PCEQvF2= NVv2UItrOTZ3MU[0O|M>
NIH3T3 Ml:wSpVv[3Srb36gRZN{[Xl? NVnpWGp1OC5{IN88US=> MYWyJIg> MW\EUXNQ NITMUIxKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? NIXMUXYyPjR6MEK4OC=>
COS7 NHLUOXBHfW6ldHnvckBCe3OjeR?= NUjSSmcyQTZizszN M4frVWlvcGmkaYTpc44hd2ZiaIXtZY4hUkGNMjDWOlE4TiCvdYThcpQh\XiycnXzd4Vl M2Hm[|E4OTd6N{Ky
A549 Mny4R5l1d3SxeHnjJGF{e2G7 M3juS|k3KGh? MW\EUXNQ M2rWcWlEPTB;MUOuOVkh|ryP MWexPFMyOzhyNx?=
K562 NIfyW2NEgXSxdH;4bYMhSXO|YYm= MYC5OkBp M{TOSmROW09? MULJR|UxRTlwM{[g{txO M{Cy[lE5OzF|OEC3
MDA-MB-231 MULDfZRwfG:6aXOgRZN{[Xl? MoP2PVYhcA>? M2XzSGROW09? M2rFbmlEPTB;M{[uOlEh|ryP Ml7mNVg{OTN6MEe=
MOLT4 MVPDfZRwfG:6aXOgRZN{[Xl? NV\RcosyQTZiaB?= NYju[lZpTE2VTx?= NHLwTnRKSzVyPUG1MlAzKM7:TR?= NX20doFiOTh|MUO4NFc>
MCF7 NUPrcIhbS3m2b4TvfIlkKEG|c3H5 MlPUOVAh|ryP MX23NkBp MWXJR|UxRTF{LkC1JO69VQ>? MYqxPFc4OThzOR?=
BT474 NHvvd4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXObYI4OiCq MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWNEe0JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWUQnKyJJdqfGhiRVO1NEBw\iByLkOg{txO M1vXd|E6ODJ6NEK0
HN5 NXu0bmdUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYW3NkBp M3jDNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO MX2xPVAzQDR{NB?=
BA/F3 MoO1R5l1d3SxeHnjJGF{e2G7 MVe3NkBp M2n4ZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> Mn23NVkzOzl{Mkm=
NIH3T3 MkD0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLCRXdEPzJiaB?= MlvWS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> MVyxPVY3PTN5Nx?=
MCF7 MX NWruSm1qTnWwY4Tpc44hSXO|YYm= NF3yfXQ{OCCvaX6= MVTJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJJdqfGhiSVO1NEBw\iBzLkGg{txO NYrIRXRIOTl7M{K5OlA>
A431 NHLYZm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXIUY4yOCEQvF2= M2TMXFczKGh? NEjxTGhIUTVyPUCuOVE1KM7:TR?= NXjWfpMxOTl7Nkm0OlU>
A431 MXzLbY5ie2ViQYPzZZk> NIewelIxNjFizszN MnfsNUBp NVTLTGNTUW6qaXLpeIlwdiCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? MmXzNVk6Pjl2NkW=
A431 NX\zUmc3U2mwYYPlJGF{e2G7 MYmxNFAh|ryP M1myUVExKG2rbh?= Ml\0VJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> M1rKbFE6QTZ7NE[1
Sf9 MUHLbY5ie2ViQYPzZZk> NGjxe3gyOCCvaX6= NGnm[INFVVOR MV7Jcohq[mm2aX;uJI9nKEWJRmKg[ZhxemW|c3XkJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdoVk\XC2b4KgZZV1d3Cqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wN|Mh|ryP NFnIVIEzODB3NkSyOS=>
NCI-H1975 Mnf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEL0PHY2KM7:TR?= MYm3NkBp NEDOO5lKSzVyPUiuNlYh|ryP Ml7ZNlAyPTF4N{C=
NCI-H1975 M3GwOWtqdmG|ZTDBd5NigQ>? M2HWW|ExKM7:TR?= MorPTY5pcWKrdHnvckBw\iCHR1\SJIF2fG:yaH;zdIhwenmuYYTpc44> M3nQXFIxOTVzNkew
NCI-H1975 M172NWZ2dmO2aX;uJGF{e2G7 NH;IOlkyOCEQvF2= MVXJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> MVOyNFE2OTZ5MB?=
NCI-H1975 Mm\wSpVv[3Srb36gRZN{[Xl? MX[1JO69VQ>? NWexVWgyPDhiaB?= M{C5U2lv\HWldHnvckBw\iCjcH;weI9{cXNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iClYYPwZZNmNTN? MXiyNFE2OTZ5MB?=
A375 NEfZXIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX:3VXhQPDhiaB?= MmH1TWM2OD1{MD61OUDPxE1? NEHESZozODNyNEWzOy=>
SMMC7721 MorNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfXOFghcA>? NIfOUFVKSzVyPUOzMlEzKM7:TR?= M2jWSVIxOzB2NUO3
DU145 MX;DfZRwfG:6aXOgRZN{[Xl? Ml\YOUBl M2nLVmROW09? NHnDOoVKSzVyPUSyMlE4KM7:TR?= M{nDd|IxPDZ4NUW1
MIAPaCa2 MnrmR5l1d3SxeHnjJGF{e2G7 Mmf0OUBl M{\MdWROW09? MnrWTWM2OD13MD62NkDPxE1? M3z4T|IxPDZ4NUW1
PANC1 NWDRTVduS3m2b4TvfIlkKEG|c3H5 Mn\WOUBl M1n4VmROW09? NEKzbYJKSzVyPUSwMlQzKM7:TR?= NE\YTYUzODR4NkW1OS=>
PC9 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\zdFdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN M2nuSVIyOjB6OECy
PC9 M4HmWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHfyc5d1cC2{ZYPpd5RidnRiaIXtZY4hWEN7IHPlcIx{KGW6cILld5NqdmdiRVfGVkBGPzR4X1G3OVAwXDd7MF2gcZV1[W62IIfpeIghTUN3MDDv[kA4KM7:TR?= NWr6UlNXOjF{MEi4NFI>
PC3 NE\hU|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzvNHYyOCEQvF2= NHrXZpQ4OiCq MnX0SG1UVw>? MYjJR|UxRTdwNDFOwG0> M4q0SlIyOzV|NUS2
MDA-MB-468 M{\Rdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrnNVAxKM7:TR?= MmXUOFghcA>? MoXoSG1UVw>? MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eR?= Mk\DNlE1Ojl4M{K=
Plasmodium falciparum MlOydWhVWyCycn;mbYxqdmdiZn;yJIlvcGmkaYTvdpMhd2ZiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJJBzd2yrZnXyZZRqd25? NVPzZY9SOjF6MUewOFU>
NCI-H1993 NHTHXm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXMTJE4PzJiaB?= NG\wNYVFVVOR MWrJR|UxRTBwMTFOwG0> MXeyNVkyPjR|Mx?=
NCI-H292 NV\PNJNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XHRlczKGh? NF;VOlFFVVOR NEXX[JBKSzVyPUCuNFIh|ryP NGXQd2czOTlzNkSzNy=>
A431 M2rz[mtqdmG|ZTDBd5NigQ>? NHvnT4gzKGh? NUDmfm1ZTE2VTx?= NVHDSI85UW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= NFnhUIEzOjFyMUGzNi=>
MCF7 MnfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIj2dVEyODEEoN88[{9uVA>? MkXEOFghcA>? NGHkbHpFVVOR NFjCVFRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> NWrVe28xOjJzMUmxN|A>
BT474 NVHWOos5S3m2b4TvfIlkKEG|c3H5 MoPZNVAh|ryP MkLSOFghcA>? MnXwR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= NYfiPGtYOjJzM{izNFg>
MCF7 MlPLR5l1d3SxeHnjJGF{e2G7 MmHWNVAh|ryP NG\VZ5E1QCCq NFvJWZBEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz M3;kdlIzOTN6M{C4
HCC827 NV\MdFB4U2mwYYPlJGF{e2G7 MUWwMlEzPSEQvF2= MlqyNlQhcA>? M1HZb2ROW09? NV3TSpRNUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62IGmxNFY5KGG3dH;wbI9{eGixconsZZRqd25? NInW[4YzOjN|OUO0Ni=>
HCC827 M4qyW2tqdmG|ZTDBd5NigQ>? M4HDRVAvOTJ3IN88US=> MnvRNlQhcA>? MmrjSG1UVw>? NEjnbGdKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= M{PIc|IzOzN7M{Sy
HCC827 NF7YeGtMcW6jc3WgRZN{[Xl? MmPmNE4yOjVizszN M3HaTVI1KGh? M176RmROW09? M{DkNmlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w M2nafVIzOzN7M{Sy
A431 NEPDToJMcW6jc3WgRZN{[Xl? MVixNFAh|ryP NXjSd3pnOzBibXnu MXvJcohq[mm2aX;uJI9nKEWJRmKg[IVzcX[nZDDmdo9uKGi3bXHuJGE1OzFiY3XscEBu\W2kcnHu[UBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJBwdHlvR3z1MXR6eiCyaH;zdIhwenmuYYTpc44hf2m2aDDJR|UxKG:oIEGuNFgh|ryP M2DtS|IzPjl6N{iy
H460 NVX6R3RmS3m2b4TvfIlkKEG|c3H5 MoPnO|IhcA>? M{jWcWlEPTB;NT61PUDPxE1? NGe1VowzOjd{N{S0PS=>
HT-29 NILFSVdEgXSxdH;4bYMhSXO|YYm= NELaTYE4OiCq NFftSHJKSzVyPUOuN|Yh|ryP M4Kyd|IzPzJ5NES5
SGC7901 NWLReZBXS3m2b4TvfIlkKEG|c3H5 MXK3NkBp MmnTTWM2OD1zMD6yOkDPxE1? NX\tfZNXOjJ5Mke0OFk>
A498 NXLIPFZ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrLPFZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= MYeyNlgyQDh2OB?=
Calu3 MWDDfZRwfG:6aXOgRZN{[Xl? MoGyNVAh|rypL33M NYLZN2YxPzJiaB?= NXy3eHBjUUN3ME2xMlYh|ryP MmjMNlMyOTZzNki=
FADU NXzlSm44S3m2b4TvfIlkKEG|c3H5 NYjHd5RsOTBizsznM41N MXK3NkBp NUTYWJlvUUN3ME2wMlA2KM7:TR?= NUnId41HOjNzMU[xOlg>
HCC827 MV;DfZRwfG:6aXOgRZN{[Xl? MmfHNVAh|rypL33M MV:3NkBp Ml7sTWM2OD1yLkCwNVUh|ryP M4f3eVI{OTF4MU[4
PC9 NEfGRXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEGwZ2gyOCEQvHevcWw> MlXhO|IhcA>? MlW2TWM2OD1yLkCwNVQh|ryP NXvob|FMOjNzMU[xOlg>
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JVM-3 NUG3[WV3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrrWodKSzVyPUKuN|Q3PDNizszN M{fEfXNCVkeHUh?=
NCI-H2170 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfCd|NKSzVyPUKuN|Q4QDNizszN NIjGTlBUSU6JRWK=
EW-18 M{Pkcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlL3TWM2OD1{LkO1OFcyKM7:TR?= NWfHSINtW0GQR1XS
KU812 NY\iPVloT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTJwM{[3O|kh|ryP MX3TRW5ITVJ?
NY MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoL1TWM2OD1{LkO3Nlch|ryP M37PU3NCVkeHUh?=
HTC-C3 NVnEZYt7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfOZ2ZIUUN3ME2yMlQzOjZ5IN88US=> NEnrW49USU6JRWK=
CFPAC-1 MmXWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TKWmlEPTB;Mj60N|g3PCEQvF2= MWfTRW5ITVJ?
HH NXjW[WFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPkbWRKSzVyPUKuOFk5PTRizszN NHfxd|hUSU6JRWK=
MKN7 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPMcIRKSzVyPUKuOlI4PDhizszN NHjNV4NUSU6JRWK=
TE-8 MnTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHI[Yp[UUN3ME2yMlY{PjV|IN88US=> NHW1S|FUSU6JRWK=
MC-IXC M{iwdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PVTmlEPTB;Mj62OFQ2OyEQvF2= MmLjV2FPT0WU
DMS-79 NXvWNWc6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7yTWM2OD1{Lk[5Nlg1KM7:TR?= MYLTRW5ITVJ?
Detroit562 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjPNXBKSzVyPUKuOlk5PDVizszN M{H1e3NCVkeHUh?=
HuP-T4 MlvGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGGxbFZKSzVyPUKuO|I5OThizszN MnOwV2FPT0WU
QIMR-WIL Mm\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXBTGZKSzVyPUKuO|U6PTJizszN MkWyV2FPT0WU
BB65-RCC MlfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInBOpFKSzVyPUKuPFA4OTNizszN NEXSOWZUSU6JRWK=
PC-3 NFK2dJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUe5eYZNUUN3ME2yMlg{OzF5IN88US=> NFnlfG9USU6JRWK=
OVCAR-3 M{j1U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXRXFRNUUN3ME2yMlg2PTl2IN88US=> NUjsblVCW0GQR1XS
KYSE-510 NF3KN4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTMR2xKSzVyPUKuPFY4QDZizszN M3j0[3NCVkeHUh?=
HC-1 NWHCS2ExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrxVpBoUUN3ME2yMlk1ODF{IN88US=> NWTkVHB4W0GQR1XS
NCI-H1693 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PYUWlEPTB;Mj65OVg6PyEQvF2= Mo\PV2FPT0WU
DU-4475 NVfqNJFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTJwOUmwOVIh|ryP NHLyT3JUSU6JRWK=
COLO-680N MkjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTNwMEC0PVUh|ryP M{TqWXNCVkeHUh?=
MN-60 NWjwS|RjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGftdFBKSzVyPUOuNFQ6PzRizszN MYrTRW5ITVJ?
BCPAP MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTNwMEiyPVUh|ryP NX7QSoFEW0GQR1XS
647-V MnfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTNwMU[yNkDPxE1? M3H6XHNCVkeHUh?=
HT-1376 NHTvSodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fzfWlEPTB;Mz6yNFUxPiEQvF2= M{[5RXNCVkeHUh?=
NCI-H1793 NHHV[GNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHybJc4UUN3ME2zMlIyODl6IN88US=> Ml70V2FPT0WU
SW1463 NECy[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTNwMk[xPVIh|ryP M3naZnNCVkeHUh?=
NCI-H727 MknCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DUfmlEPTB;Mz6zPFU5PCEQvF2= MnT4V2FPT0WU
LB771-HNC M4mwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2e3bGlEPTB;Mz6zPVEzPiEQvF2= M3XmXnNCVkeHUh?=
D-542MG MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTNwNESyNlIh|ryP NULnZY94W0GQR1XS
NTERA-S-cl-D1 M{HkS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTNwNES5OVch|ryP MnryV2FPT0WU
C8166 MnvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTNwNE[1NlEh|ryP M1XBfXNCVkeHUh?=
639-V NH7MTWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;QfJJnUUN3ME2zMlU4Pzl{IN88US=> NWXWOpFuW0GQR1XS
TE-1 NUnsepNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTNwNUmxOlkh|ryP MkKyV2FPT0WU
OAW-28 M2fp[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nQeGlEPTB;Mz61PVQzPiEQvF2= MojiV2FPT0WU
SK-LU-1 MnvQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LYRWlEPTB;Mz62NFY1PSEQvF2= NV63RVBMW0GQR1XS
CaR-1 NITMZnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXT0PFJzUUN3ME2zMlYyODR6IN88US=> MlTHV2FPT0WU
A2780 MkLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTNwNkSwPFkh|ryP MlLUV2FPT0WU
C2BBe1 M1LVSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLwWnNiUUN3ME2zMlY1PDB5IN88US=> NFr5VodUSU6JRWK=
SK-MES-1 NXv3SJM2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnGzTWM2OD1|Lk[0OlE1KM7:TR?= NH;xUJlUSU6JRWK=
PANC-10-05 NFLKToNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PsSGlEPTB;Mz63NlA4OiEQvF2= NWHEO3JuW0GQR1XS
J-RT3-T3-5 NWflWpVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInLOXVKSzVyPUOuO|U1ODZizszN NXPSOZdZW0GQR1XS
ES4 MkfZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;JVpZKSzVyPUOuPFIzQDZizszN NVHjcnZDW0GQR1XS
NCI-H28 M1PueGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXX5Z2pnUUN3ME2zMlgzQTZzIN88US=> MYnTRW5ITVJ?
NCI-H1155 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HnfmlEPTB;Mz64OFE2QSEQvF2= MWDTRW5ITVJ?
KURAMOCHI M{i3OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M36wSmlEPTB;Mz64OlA2PiEQvF2= MlzNV2FPT0WU
NCI-H69 MojsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{n2fWlEPTB;Mz65NFE4QCEQvF2= MX3TRW5ITVJ?
IST-MES1 NEeySWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTNwOUSwNlkh|ryP MVLTRW5ITVJ?
NH-12 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTNwOUSxPFch|ryP NUO3Z2VzW0GQR1XS
JVM-2 MljDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITvWJlKSzVyPUOuPVQ4PTJizszN M{j3[HNCVkeHUh?=
EW-16 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nvVWlEPTB;Mz65OVU4PyEQvF2= M{XRNHNCVkeHUh?=
NCI-H441 NWC4NnNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3GyW2lEPTB;Mz65O|AyPCEQvF2= NVPteIMxW0GQR1XS
RCC10RGB M13id2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHZTWM2OD12LkCxNlQ3KM7:TR?= NUnWNWR5W0GQR1XS
TCCSUP MmnWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEG1TWlKSzVyPUSuNVExPSEQvF2= MWjTRW5ITVJ?
HL-60 NVH6NnlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzIV2RqUUN3ME20MlEzOjRzIN88US=> MorLV2FPT0WU
EW-11 MlTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PYTWlEPTB;ND6xN|I3OSEQvF2= NULoR2U2W0GQR1XS
KARPAS-299 M4njZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1G3U2lEPTB;ND6zNVAyOyEQvF2= NF7SNIRUSU6JRWK=
SNU-C2B NIe5[3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLwTWM2OD12LkO0Olg3KM7:TR?= NGrFVoZUSU6JRWK=
LU-139 M4n5T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnT6TWM2OD12LkO3NlQ4KM7:TR?= NEDSd4FUSU6JRWK=
HCC1937 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTRwM{e5PVMh|ryP MXzTRW5ITVJ?
GR-ST MoDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTRwNEOxPEDPxE1? MX7TRW5ITVJ?
SK-OV-3 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofYTWM2OD12LkS2NFQzKM7:TR?= MYrTRW5ITVJ?
A172 M17rZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTRwNUW1NlEh|ryP NUC0cYJLW0GQR1XS
NB10 M{f0NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlmzTWM2OD12LkW5O|gyKM7:TR?= NWXER2g4W0GQR1XS
P30-OHK MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTRwOEG5Nlkh|ryP MorKV2FPT0WU
KYSE-520 Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zqWmlEPTB;ND64PFAyQCEQvF2= MVrTRW5ITVJ?
KNS-62 NVTLdIlQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3f1TWlEPTB;ND65NFgxPyEQvF2= MXXTRW5ITVJ?
ES1 MmfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF6yUHpKSzVyPUSuPVYxOzVizszN MWrTRW5ITVJ?
KOSC-2 MmXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTRwOUm5OVkh|ryP Ml6yV2FPT0WU
TE-9 MkfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTVwMEG1NlUh|ryP MWjTRW5ITVJ?
NCI-H1838 NILJWFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnTfopKSzVyPUWuNFIxPzZizszN MXTTRW5ITVJ?
KP-N-YN M1fUeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3ZXFVKSzVyPUWuNVI2QTFizszN MVzTRW5ITVJ?
ALL-PO NEnTTWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\5cGlEPTB;NT6yNlU5PyEQvF2= NU\oN|ZxW0GQR1XS
NOMO-1 NXnYVGtGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzESpZjUUN3ME21MlI{OTd4IN88US=> NUHhRldRW0GQR1XS
CGTH-W-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUS3S5hMUUN3ME21MlM5PTh2IN88US=> NFnNZ5lUSU6JRWK=
ATN-1 MofMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHXTYhlUUN3ME21MlQyOTh2IN88US=> MnXDV2FPT0WU
AGS MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlmxTWM2OD13LkS5OlE5KM7:TR?= NULCU4hyW0GQR1XS
SW13 Mnn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvlTWM2OD13LkWzNlY2KM7:TR?= M3fjWHNCVkeHUh?=
SW1990 M1Labmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLZTWM2OD13Lk[yNlg2KM7:TR?= NU\NR2ZIW0GQR1XS
BFTC-905 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTVwNkW4O|gh|ryP MkTYV2FPT0WU
RMG-I Mn24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2GwTGlEPTB;NT62OVk1OiEQvF2= M4PHXXNCVkeHUh?=
HD-MY-Z MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\HWpo5UUN3ME21MlY5PThzIN88US=> M4K1NXNCVkeHUh?=
HCC1187 NILMVZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmL0TWM2OD13LkexNFMzKM7:TR?= M2LTcHNCVkeHUh?=
HCC2998 NF7nTXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXQTWM2OD13LkexPFc4KM7:TR?= M3WyVnNCVkeHUh?=
RT-112 NGrZZ29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGT2cG5KSzVyPUWuPVAzPDVizszN NX\sXWVMW0GQR1XS
CAKI-1 M2TBVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTZwMEC5PFUh|ryP NEXERZpUSU6JRWK=
HDLM-2 M1\XNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LY[2lEPTB;Nj6wNVQ5QSEQvF2= NHvVXWtUSU6JRWK=
TE-10 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTZwMES4N|Mh|ryP NX22N4R2W0GQR1XS
KE-37 NU\GWWZZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTZwMEeyOVgh|ryP MVjTRW5ITVJ?
RCM-1 M2fW[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\STWM2OD14LkC3O|E6KM7:TR?= MlfvV2FPT0WU
Mewo MoGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvYTWM2OD14LkGxOFg4KM7:TR?= M{jlXXNCVkeHUh?=
L-428 NE\Yb5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnsVmhMUUN3ME22MlE5ODN5IN88US=> NUGwO5FiW0GQR1XS
8-MG-BA MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrkNlIyUUN3ME22MlE6Ozd7IN88US=> NWj5SFVuW0GQR1XS
SK-MEL-28 NG[3SXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVyzXmtmUUN3ME22MlIxQTZ5IN88US=> NVHZfG9HW0GQR1XS
VA-ES-BJ MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2GxXmlEPTB;Nj6zNFQ3OiEQvF2= MWnTRW5ITVJ?
CAL-120 M3fzUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUS2cm5QUUN3ME22MlQ2OTZ{IN88US=> M1\wU3NCVkeHUh?=
GAMG NFrsO45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTZwNUC2NkDPxE1? MlzhV2FPT0WU
MSTO-221H NX\DS5F[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHmzdllKSzVyPU[uOVEyPyEQvF2= M4DiN3NCVkeHUh?=
GCT MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfmV|BKSzVyPU[uOVM{QDdizszN MWXTRW5ITVJ?
SH-4 NVfMeoJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTZwNUi4OFQh|ryP Mm\TV2FPT0WU
Calu-3 NEjIenpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DZZ2lEPTB;Nj62OVU3QCEQvF2= M{TMOXNCVkeHUh?=
SK-HEP-1 M2LWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDRTWM2OD14LkexO|U{KM7:TR?= NX3vN25YW0GQR1XS
PFSK-1 M1PD[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHToW|NKSzVyPU[uO|M1PzhizszN M4n6c3NCVkeHUh?=
NB12 NWS4eYRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTZwN{i0NFQh|ryP NEXyTWhUSU6JRWK=
A388 Mmi1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;QTWM2OD14Lki5OVIh|ryP NYnycVVPW0GQR1XS
KLE Mor5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mly3TWM2OD14Lkm2OVE3KM7:TR?= NYrqe41[W0GQR1XS
TE-11 NH\y[|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPWSIc2UUN3ME23MlA1QTZ5IN88US=> NX;3UJVlW0GQR1XS
NCI-H2405 Ml3ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLKV5JKSzVyPUeuNFg{QTNizszN MXrTRW5ITVJ?
OVCAR-4 NH2xV5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXaTFFKSzVyPUeuNVU{PSEQvF2= MYjTRW5ITVJ?
KP-N-YS MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fmOWlEPTB;Nz6xPFE1KM7:TR?= NYHBSppGW0GQR1XS
NOS-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHyTWM2OD15LkK1PFI5KM7:TR?= NGfvb2xUSU6JRWK=
DMS-114 M{G5cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTdwMkm2N|Eh|ryP MmD0V2FPT0WU
NCI-H2030 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTdwM{O2PVgh|ryP MX\TRW5ITVJ?
HEL MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\xTWlEPTB;Nz6zO|UzOSEQvF2= M3\leHNCVkeHUh?=
CAL-62 M1rs[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\yeGo3UUN3ME23MlM6QDZ4IN88US=> NIXJNHFUSU6JRWK=
COLO-668 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTdwNEO5Olch|ryP MYnTRW5ITVJ?
SKG-IIIa M3:3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTdwNEWzPFEh|ryP MnrJV2FPT0WU
HCC1419 MoHCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfFR4E4UUN3ME23MlQ3QDN|IN88US=> Mn[yV2FPT0WU
OS-RC-2 M4HaOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vncGlEPTB;Nz60PVg6PCEQvF2= NICyRmJUSU6JRWK=
COLO-829 NWC1b2tOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvtNIdKSzVyPUeuOlY1PjNizszN MnTKV2FPT0WU
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NB14 NHTwOJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjFSW9KSzVyPUO4MlQ2PDRizszN NFS2SVJUSU6JRWK=
D-423MG MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MontTWM2OD1|OD60PFY6KM7:TR?= NGHNZnJUSU6JRWK=
NCI-H510A NEK0S5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTN6Lk[0OVEh|ryP MULTRW5ITVJ?
NMC-G1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\TbYRKSzVyPUO4MlczOjJizszN NHPIUnVUSU6JRWK=
SW948 MlLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvVOoZJUUN3ME2zPE45QTR7IN88US=> MXrTRW5ITVJ?
MFM-223 NXfYVGNuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4j3e2lEPTB;M{iuPVQ{OSEQvF2= MW\TRW5ITVJ?
PAN1 NHnZSHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moj4TWM2OD1|OD65PVE6KM7:TR?= Mo[2V2FPT0WU
MZ7-mel NILmbFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjINnlNUUN3ME2zPU4yOTl7IN88US=> MmO3V2FPT0WU
COLO-679 M3q0Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPrTVRKSzVyPUO5Mlk4ODhizszN NUSyVVU2W0GQR1XS
SW872 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfT[IZmUUN3ME20NE4zODNzIN88US=> NH3JNVJUSU6JRWK=
HT-1197 NH;TPZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYKxPHdXUUN3ME20NE4zQDZ7IN88US=> M4PPOXNCVkeHUh?=
ES6 NYDXcHg6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DDV2lEPTB;NECuOFU2OiEQvF2= NYXXXVBpW0GQR1XS
SNU-387 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnSbGJKSzVyPUSwMlgzOzhizszN MliwV2FPT0WU
T47D M2raRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfCTnU6UUN3ME20NU4xOTl{IN88US=> M2j6OnNCVkeHUh?=
G-402 NVG1OZl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTRzLkC4NFkh|ryP NGWwcHlUSU6JRWK=
MZ1-PC NF\0c2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTRzLkixJO69VQ>? M1jBPHNCVkeHUh?=
SW1417 MnjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\LeGlEPTB;NEKuN|A{KM7:TR?= MmfVV2FPT0WU
RERF-LC-MS NVnQcIZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnCXIk{UUN3ME20Nk42ODh5IN88US=> M3PyTHNCVkeHUh?=
SF539 M3XQc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fycmlEPTB;NEKuOVUzQSEQvF2= MUfTRW5ITVJ?
ESS-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTR|LkW1OVgh|ryP M1L3S3NCVkeHUh?=
RO82-W-1 NGDwWXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLaTWM2OD12Mz62NVU{KM7:TR?= NXrU[llyW0GQR1XS
S-117 M3TJbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTR|Lk[5OVgh|ryP M{m4PHNCVkeHUh?=
SW756 MljkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLud4JHUUN3ME20N{45OjR{IN88US=> MXzTRW5ITVJ?
CP66-MEL M3XGbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjHeGw4UUN3ME20OU44OTR|IN88US=> M3PvSHNCVkeHUh?=
H-EMC-SS M4TvfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTR3Lke2PVMh|ryP M3y2bnNCVkeHUh?=
NCI-H2052 MlXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTR3LkmwNlch|ryP NYTQPINiW0GQR1XS
SW684 NFTUdWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXJ[2lKSzVyPUS3MlAxQDFizszN M1XM[HNCVkeHUh?=
HCC1569 NHPWe5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTR5LkG2OVgh|ryP MlXoV2FPT0WU
KGN NGLPSnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TuTWlEPTB;NEeuN|MyOSEQvF2= MXXTRW5ITVJ?
LCLC-103H NGXPN5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH70ZYZKSzVyPUS3MlQ1ODRizszN NH6xem9USU6JRWK=
SW780 NV6xN2RNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\0RYF{UUN3ME20O{43OzVzIN88US=> NE\Jc2hUSU6JRWK=
NCI-H1703 M1vVUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTR6LkOzOVIh|ryP NEnjXYhUSU6JRWK=
HCC1395 NX7ufYljT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTR6Lke0N|Ih|ryP NXfWPGZPW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03720873 Recruiting EGFR Gene Mutation Fujian Cancer Hospital October 2018 Phase 2
NCT03599518 Recruiting Non Small Cell Lung Cancer Daiichi Sankyo Co. Ltd.|Daiichi Sankyo Inc. October 10 2018 Phase 1
NCT03653546 Not yet recruiting Non-small Cell Lung Cancer|EGFR Gene Mutation|Brain Metastases Alpha Biopharma (Jiangsu) Co. Ltd. August 31 2018 Phase 2|Phase 3
NCT03602027 Not yet recruiting Non-squamous Non-small Cell Lung Cancer Second Affiliated Hospital of Nanchang University August 1 2018 Not Applicable
NCT03656393 Recruiting NSCLC|EGFR Shenzhen People''s Hospital August 31 2018 Phase 3
NCT03529084 Withdrawn Carcinoma Non-small Cell Lung Novartis Pharmaceuticals|Novartis July 23 2018 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID