Fulvestrant (ICI-182780)

For research use only.

Catalog No.S1191 Synonyms: ZD 9238, ZM 182780

95 publications

Fulvestrant (ICI-182780) Chemical Structure

CAS No. 129453-61-8

Fulvestrant (ICI-182780, ZD 9238, ZM 182780) is an estrogen receptor (ER) antagonist with IC50 of 0.94 nM in a cell-free assay. Fulvestrant also induces autophagy and apoptosis and has antitumor activity.

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Selleck's Fulvestrant (ICI-182780) has been cited by 95 publications

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Fulvestrant (ICI-182780, ZD 9238, ZM 182780) is an estrogen receptor (ER) antagonist with IC50 of 0.94 nM in a cell-free assay. Fulvestrant also induces autophagy and apoptosis and has antitumor activity.
Targets
ER [1]
(Cell-free assay)
0.94 nM
In vitro

Fulvestrant is an effective inhibitor of the growth of ER-positive MCF-7 (with IC50 of 0.29 nM) but with no effect on the growth of ER-negative BT-20 human breast cancer cells. Fulvestrant causes accumulation of cells in G0/G1 and also reduces the proportion of cells capable of continued DNA synthesis. [1] Fulvestrant competitively inhibits binding of oestradiol to the estrogen receptor. Fulvestrant blocks nuclear localization of the ER through impairing receptor dimerisation, and energy-dependent nucleo-cytoplasmic shuttling. Because of the instability of fulvestrant-ER complex, the binding of Fulvestrant with ER finally results in accelerated degradation of the ER protein. [2] Fulvestrant (10 nM) not only decreases IGF-IR mRNA levels but also decreases the half-life. [3] Treatment with 100 μM Fulvestrant leads to a time dependent increase of TNFR1 and TRADD steady-state mRNA levels in MCF-7 cells. [4] Fulvestrant is capable of down-regulating androgen receptor expression and diminishes androgenic responses in LNCaP human prostate cancer cells. Fulvestrant also significantly attenuates R1881-stimulated growth by 70%. [5] Fulvestrant is able to modulate mitosis and cell death in immature cerebellar neurons via rapid activation of MAPK. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 NW[wNJJkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUixMlMuOTByMDDuUS=> M2fLeFQ5KGh? MVvpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl5OUK5OEc,OjR7N{myPVQ9N2F-
MCF-7/LTED MmnTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1n1UFEvOy1zMECwJI5O MnPrOFghcA>? Moi1bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1HBclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUe5Nlk1Lz5{NEm3PVI6PDxxYU6=
HCC1428 NGC1bpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYmxMlMuOTByMDDuUS=> MkDwOFghcA>? MkDubY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M2LSO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUe5Nlk1Lz5{NEm3PVI6PDxxYU6=
HCC1428/LTED M4ntN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCxMlMuOTByMDDuUS=> NEPtbmE1QCCq NFS5cFRqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M2\VbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUe5Nlk1Lz5{NEm3PVI6PDxxYU6=
LCC1 NETvSHFHfW6ldHnvckBCe3OjeR?= NI\GUHAyODBibl2= M{HaWVQ5NTF2NDDo NVfrXIE1[WO2aY\heIV{KFWSUjDzbYdv[Wyrbne= NYfwUG9sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OVgzPzdpPkK0PFU5Ojd5PD;hQi=>
LCC9 MmTKSpVv[3Srb36gRZN{[Xl? MkjVNVAxKG6P MmfVOFguOTR2IHi= NVvwVXlb[WO2aY\heIV{KFWSUjDzbYdv[Wyrbne= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh3OEK3O{c,OjR6NUiyO|c9N2F-
MCF-7  NYDRPWZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXSU|lKOTByIH7N NYfCWYZbPSCm NUDvOXZOcW6qaXLpeJMh[2WubDDndo94fGhidH:gNVDwxIV? NXT0dnNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVk2PTBpPkK0PFE6PTVyPD;hQi=>
mesangial  NUTqem1jTnWwY4Tpc44hSXO|YYm= MkfWNE4yNTFyMDDuUS=> NHnIUI41QCCq NFj4XI1{fXCycnXzd4V{KFSJRj5OtlEucW6mdXPl[EB1gXCnIFnWJINwdGyjZ3Xu NXLQSVUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PVM3OzlpPkK0O|k{PjN7PD;hQi=>
Mesangial NFLyVXNHfW6ldHnvckBCe3OjeR?= MoPqNE4yNTFyMDDuUS=> MYSwMlUhcA>? NVXRbI0{cW6qaXLpeJMhXEeILd8yNU1qdmS3Y3XkJHNu[WR{IIDoc5NxcG:{eXzheIlwdiC4aXGgS3BGWg>? M2\hd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{mzOlM6Lz5{NEe5N|Y{QTxxYU6=
ER+ MCF-7/2a M17QR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEftWGJKSzVyPUCuNFA1KM7:TR?= M4jKPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3M{K0PFg1Lz5zNUOyOFg5PDxxYU6=
ER+ MCF-7 M3zCVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfOdFQzODBiaB?= NV;6eZE1UUN3ME2wMlIyKG6P NW\F[IRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWzNlQ5QDRpPkG1N|I1QDh2PD;hQi=>
MCF-7  NF35dVNHfW6ldHnvckBCe3OjeR?= NILjXGIyOMLibl5CpC=> MWq3NkBp M1X0fpJmfmW{c3XzJJRp\SCnc4Tyc4dmdiCnZn\lZ5QpUUN3MDCxMlkhy5diMUFijLI6KE1r MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyOE[1Nkc,OjR7MEi2OVI9N2F-
MCF-7  MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxyqCxZjDhdJBzd3irbXH0[Yx6KDJibl2= NWnGUY1FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFg{PDZpPkKzOFQ5OzR4PD;hQi=>
H1975  NUX1d29XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV6zxsDPxE1? MnfjNUBu MnTubY5kemWjc3XzJJRp\SCpZX\peIlvcWJic3Xud4l1cX[rdImgc4YhUDF7N{WgZ4VtdHQEoB?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4OEixNEc,OjR{Nki4NVA9N2F-
H1975 NGSxVZZHfW6ldHnvckBCe3OjeR?= NVLNe4ZMO8LizszN NYTNRVM6OSCv NEHBVGd2eHKnZ4XsZZRmeyC2aHWgcIV3\Wxib3[gUIV1NTel NUTUXllCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlg5OTBpPkK0NlY5QDFyPD;hQi=>
MCF-7  MXvGeY5kfGmxbjDBd5NigQ>? NX\zRYhzOTBy4pEJcm3DqA>? NI[3Vpo4OiCq Mn7zdoV3\XK|ZYOgeIhmKHC{b4TlZ5RqfmViZX\m[YN1KG:oIFWyxsBqdiClZXzsJIlvfmG|aX;u Mo[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{[3O|MoRjJ|OUO2O|c{RC:jPh?=
MCF-7  NEO3Wo1HfW6ldHnvckBCe3OjeR?= NXrjZ2dCOTBy4pEJcm3DqA>? MlHmNlQwPDhiaB?= MWfmZYNqdGm2YYTld{Bqdn[jc3nvckB1cHKxdXfoJG1OWHNpIH3v[JVt[XSrb36= NF3vV5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzOlc4Oyd-MkO5N|Y4PzN:L3G+
BT474-tet-shMED1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XaNVAvOS13IN88US=> MoHiO{Bl MUfpcoNz\WG|ZYOgL2RwgCCrbnT1Z4VlKGOnbHyg[IVifGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MlnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{[yN|QoRjJ|OUO2NlM1RC:jPh?=
ZR75-1-tet-shMED1  MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3ro[lAvOS13IN88US=> M3vlblch\A>? NXHmSW44cW6lcnXhd4V{KCuGb4igbY5lfWOnZDDj[YxtKGSnYYToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|NkKzOEc,OjN7M{[yN|Q9N2F-
MCF-7-tet-shMED1 MlLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHkNE4yNTVizszN NG[yTW44KGR? MUDpcoNz\WG|ZYOgL2RwgCCrbnT1Z4VlKGOnbHyg[IVifGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl|NkKzOEc,OjN7M{[yN|Q9N2F-
HepG2  NUfFNY9ZTnWwY4Tpc44hSXO|YYm= M4PmO|AvODFvMUCg{txO M3zkNVE5KGkEoB?= NGrPc5Ni[3SrdnH0[ZMhfGinIFXSSU1u\WSrYYTl[EB1emGwc3PybZB1cW:wIH;mJGFHOkWU Mk[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5M{OxPFgoRjJ|N{OzNVg5RC:jPh?=
MCF-7L  MVrGeY5kfGmxbjDBd5NigQ>? MYOxNFAhdk4EoB?= MW[xNEBucW5xMkSgbE81QCCq NEPveWxz\XO3bITzJIlvKEWJRmKsJGhGWjJiYX7kJGhGWjNicHjvd5Bpd3K7bHH0bY9vKGG2IIDyc4xwdmenZDDlfJBwe3W{ZR?= M3j1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nki2OFE3Lz5{M{[4OlQyPjxxYU6=
MCF-7L  NYfNZ4JWTnWwY4Tpc44hSXO|YYm= MVKxNFAhdk4EoB?= NG[3T4w1QCCq NIKxbWdqdmS3Y3XzJJVxemWpdXzheIlwdiCxZjDtVm5CKG:oIFXHSnIhdGmpYX7kJGhDNUWJRh?= M{jDZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nki2OFE3Lz5{M{[4OlQyPjxxYU6=
MCF-7L MVXGeY5kfGmxbjDBd5NigQ>? M1WzSlExOCCwTdMg M1;GNlQ5KGh? MknrbY5lfWOnczDFS2ZTKG[jbXnsfUBu\W2kZYKgZYN1cX[jdHnvckBz\XG3aYLl[EBGWg>? NVT2PI1oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFY1OTZpPkKzOlg3PDF4PD;hQi=>
C4-12  NHjV[pdHfW6ldHnvckBCe3OjeR?= NHrDNpoyODBibl5CpC=> NF7JWGY1QCCq NHnMVYtqdmS3Y3XzJGVITlJiZnHtbYx6KG2nbXLldkBi[3SrdnH0bY9vKHKncYXpdoVlKEWU M{fvO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nki2OFE3Lz5{M{[4OlQyPjxxYU6=
MCF-7L NIH1XHVHfW6ldHnvckBCe3OjeR?= MX[xNFAhdk4EoB?= MYCyOEBp MnPEbY5lfWOnczDFS2ZTKHCqb4PwbI9zgWyjdHnvckBz\XG3aYLl[EBJSi2HR1[g[pVv[3Srb36= NYXJToU1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFY1OTZpPkKzOlg3PDF4PD;hQi=>
MMQ  NWL6U4J2TnWwY4Tpc44hSXO|YYm= NFHCcJMxNTZ{NTDuUS=> MnXGO|IhcA>? MYLkc5dvNXKnZ4XsZZRmeyC2aHWg[ZhxemW|c3nvckBw\iCnc4Tyc4dmdiC{ZXPldJRwei4QsTCoSXLPuSl? MoLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkOzOVcoRjJ|NUKzN|U4RC:jPh?=
H1975  M4jOUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWCyeVRROC5|MUK1MVExKM7:TR?= NILNWZM3KGR? NHzNVGxqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NYH3S2p3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPVk6PTdpPkKzN|k6QTV5PD;hQi=>
H1975  MXjBdI9xfG:|aYOgRZN{[Xl? NEPKW3MzODBibl2= NEDkbGE4OiCq NH[wN3pmdmijbnPld{BmemyxdHnubYIhcW6mdXPl[EBieG:ydH;zbZM> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN7OUm1O{c,OjN|OUm5OVc9N2F-
MCF-7  MlT2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jlUlExNzFyMD:xNFAxKG6P NX7kNZY6Oi92L{[g[C=> MX;EUXNQ NIXxNnVqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy Mm\3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MUO1NFYoRjJ|M{GzOVA3RC:jPh?=
MCF-7  NY\ofWoyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrkR3ZrOTByIH7N NI[zTWY1KGR? NWTjPI4xTE2VTx?= MV\pcoR2[2W|IHGgbIlocGW{IIDyc5BwenSrb36gc4Yh[2WubIOgbY4hfGinIFexJJBp[XOnwrC= MlyxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MUO1NFYoRjJ|M{GzOVA3RC:jPh?=
MLO-Y4  NIX4VXpHfW6ldHnvckBCe3OjeR?= M1i3Z|HDqM7:TR?= M13pOVEhcA>? NXjxVm5XcW6qaXLpeJMhTTJvaX7keYNm\CCFeESzJIV5eHKnc4Ppc44> M4rZS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkS3NFU4Lz5{M{K0O|A2PzxxYU6=
MCF-7 MnL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIf4[o8yODBibl2= MmXtOFghcA>? MYfhZpJw\2G2ZYOgeIhmKHC{b3zp[oVz[XSrdnWg[YZn\WO2IH;mJI1w\GW{YYTlJI5qfHKxc3H0bZZmKHO2cnXzdy=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJzNke0OEc,OjN{MU[3OFQ9N2F-
TG1-1  MmHaSpVv[3Srb36gRZN{[Xl? MX[xxsDPxE1? MUGyOEBp NH;SXnNi[nKxZ3H0[ZMhTTMEoHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEiLRj2x{tE> NVWxN|luRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwPFg3ODdpPkKzNFg5PjB5PD;hQi=>
TG1-1  NWK4bYRKTnWwY4Tpc44hSXO|YYm= M{G3dlHDqM7:TR?= NWfmPVJJOjRiaB?= M4nSNIFjem:pYYTld{BGOsLiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhWEl|Sx?= NES2S|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C4PFYxPyd-MkOwPFg3ODd:L3G+
MCF7 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\2SIUyODBibl2= M4W2VlQ5KGh? NFn2XIpt\WGmczD0c{BiKHOrbXnsZZIhdG:|czDpckB{fXK4aY\hcEBieyC5aYToJIRwgG:{dXLpZ4lvKGGub37l NYSzcmVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|czPDlpPkKzNFc4OjR7PD;hQi=>
MCF7 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIq4eZQyODBibl2= M4TmVlQ5KGh? MV;lcohidmOnczDueZRtcW5vbXXkbYF1\WRiY3XscEBl\WG2aB?= M{DaT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEe3NlQ6Lz5{M{C3O|I1QTxxYU6=
MCF-7  M1zpV2Z2dmO2aX;uJGF{e2G7 MnTnOkBp NGLpfHBFVVOR NHO1emVifHSnboXheIV{KHSqZTDmcJVlcW:6b37pcE0hd3JiZnXubIV5[W2rZD3pcoR2[2WmIHnuZ5Jm[XOnIHnuJI1qWi1{MTDlfJBz\XO|aX;u NU\4U2ZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOVIxOzZpPkKzNFUzODN4PD;hQi=>
MCF-7 NVLDOndGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEO0b4UyODBibl2vNUDPxE1? NVGxWYNkPSCm MXvpcohq[mm2czD0bIUhe3SrbYXsZZRqd25ib3[gNVfPui2nc4TyZYRqd2x? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl6Mke2OUc,OjJ7OEK3OlU9N2F-
MCF-7 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jBcFExOCCwTR?= M3HsdlUh\A>? M3HObolvcGmkaYTzJJRp\SC|dHnteYxifGmxbjDv[kBnfXOjcnnlcIlvKEh? MojsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OEK3OlUoRjJ{OUiyO|Y2RC:jPh?=
1471.1 MXfGeY5kfGmxbjDBd5NigQ>? MknDNVAxKG6P NUPvc5pLOSCq Mnn2SZRQUMLi NIHJVoh1[WunczDvckBiKHC3bnP0ZZRmKHO2YXnubY5oKHCjdITldo4> NX;IdVE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OlkyODZpPkKyPFY6OTB4PD;hQi=>
MCF-7 MYfGeY5kfGmxbjDBd5NigQ>? NWTSU2t3OTByIH7N MXuxJIg> MUPFeG9JyqB? NGLiUFB1[WunczDvckBiKHC3bnP0ZZRmKHO2YXnubY5oKHCjdITldo4> NWDrNpNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OlkyODZpPkKyPFY6OTB4PD;hQi=>
HeLa MnP2SpVv[3Srb36gRZN{[Xl? MmnMNVAxKG6P MoPPNUBp NY\2R3d{TXSRSNMg NWT6WnYxfGGtZYOgc44h[SCydX7jeIF1\SC|dHHpcolv\yCyYYT0[ZJv MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh4OUGwOkc,OjJ6NkmxNFY9N2F-
COS-7  M3:1TmZ2dmO2aX;uJGF{e2G7 MWCxNFAhdk1? M2nhblEhcA>? NFzKNppGfE:KwrC= M{nxXZRic2W|IH;uJIEheHWwY4TheIUhe3SjaX7pcocheGG2dHXyci=> NInxTYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki2PVExPid-MkK4OlkyODZ:L3G+
BG1L-OHTLT  MVPGeY5kfGmxbjDBd5NigQ>? MUGxNOKhdk1? NVzSN4JCOjUEoHlCpC=> MX7pcohq[mm2czDFVu6yKGW6cILld5Nqd25? NW\yVVBpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OVI2PThpPkKyOlUzPTV6PD;hQi=>
BG1L-ICILT NEjScI5HfW6ldHnvckBCe3OjeR?= M4nUXFExyqCwTR?= M1X5VlI1yqCqwrC= NHT0ToFqdmirYnn0d{BGWs7zIHX4dJJme3Orb36= M4Hac|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkWyOVU5Lz5{Mk[1NlU2QDxxYU6=
PC-9 M{KyV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXWwMlAxOy1|MDFOwG0> NYLNXnJQPDhiaB?= NFXXXW5qdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MkS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkC2N|QoRjJ{NU[wOlM1RC:jPh?=
H1650 Ml7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVewMlAxOy1|MDFOwG0> NW\QOIFPPDhiaB?= NFTQ[JhqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NF;wbGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW2NFY{PCd-MkK1OlA3OzR:L3G+
H1975 MoPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rmeFAvODB|LUOwJO69VQ>? NHPDXVI1QCCq NUCyUoNDcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M{frOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NU[wOlM1Lz5{MkW2NFY{PDxxYU6=
H1975 MWTGeY5kfGmxbjDBd5NigQ>? Moi3N:Kh|ryP MnvKN{Bp NFrMPHZi[nKxZ3H0[ZMhfGinIIDoc5NxcG9vRVfGVkBqdmS3Y4Tpc44h[nliZYP0do9o\W5? MnPjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkC2N|QoRjJ{NU[wOlM1RC:jPh?=
H1975 NILrUmxHfW6ldHnvckBCe3OjeR?= M3T6T|PDqM7:TR?= NGnld4E4KGR? MnHibY5lfWOnczDFS2ZTKGW6cILld5Nqd25? NWf3PIxxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OlA3OzRpPkKyOVYxPjN2PD;hQi=>
HTR-8 NW\rU3BYTnWwY4Tpc44hSXO|YYm= NGPQO2wyyqEQvF2= MWSxMVQ5KGh? NYnFXYZm\G:5bj3y[Yd2dGG2ZYOgeIhmKGW6cILld5Nqd25ib3[gTWdHSlB5IH3SUmE> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN6M{GxNUc,OjJ|OEOxNVE9N2F-
JEG-3 MoX5SpVv[3Srb36gRZN{[Xl? MV[xxsDPxE1? MnLVNU01QCCq M4HFVYRwf25vcnXneYxifGW|IITo[UBmgHC{ZYPzbY9vKG:oIFnHSmJRPyCvUl7B M17LWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{izNVEyLz5{MkO4N|EyOTxxYU6=
Huh7 M2fxeGZ2dmO2aX;uJGF{e2G7 NHzRXms2OMLizszN MlPMOFghcA>? MnG3bY5pcWKrdIOg[4VvcXO2ZXnuMY1m\GmjdHXkJHBQVjFidILhcpNi[3SrdnH0bY9v M3jydVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{C0Nlk3Lz5{MkOwOFI6PjxxYU6=
201T NH3pb5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjXOUDPxE1? MkHuO|IhcA>? M2[ze4lvcGmkaYTzJINmdGxiZ4Lve5RpKHOrZ37p[olk[W62bImgZ49u[mmwZXSge4l1cCC4YX7k[ZRidmmk NE\xeI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK1PFQ4Pid-MkKyOVg1PzZ:L3G+
A549  NV[3VGM3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jCbVUh|ryP NFXxcJM4OiCq M3P1VYlvcGmkaYTzJINmdGxiZ4Lve5RpKHOrZ37p[olk[W62bImgZ49u[mmwZXSge4l1cCC4YX7k[ZRidmmk M2jVZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkW4OFc3Lz5{MkK1PFQ4PjxxYU6=
MCF-7 MmH2SpVv[3Srb36gRZN{[Xl? NGPwXYMyyqEQvF2= M3mxOVI1KGkEoB?= MWXkc5dvemWpdXzheIV{KEWUIHX4dJJme3Orb36gbY5lfWOnZDDifUA1NU:KLWSgxsA> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB2OUOxOkc,OjJyNEmzNVY9N2F-
HCC-1428 M1zOSWZ2dmO2aX;uJGF{e2G7 NGPYZnQyyqEQvF2= MmTTNlQhcMLi NYLxOVJC\G:5boLl[5Vt[XSnczDFVkBmgHC{ZYPzbY9vKGmwZIXj[YQh[nliND3PTE1VKMLi MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB2OUOxOkc,OjJyNEmzNVY9N2F-
MDA-361 Ml32SpVv[3Srb36gRZN{[Xl? M17lcFHDqM7:TR?= MmS5NlQhcMLi MUjkc5dvemWpdXzheIV{KEWUIHX4dJJme3Orb36gbY5lfWOnZDDifUA1NU:KLWSgxsA> M2TPbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MES5N|E3Lz5{MkC0PVMyPjxxYU6=
ZR75-1 NFLwfHFHfW6ldHnvckBCe3OjeR?= NUi3O2UxOcLizszN NEGxcJMzPCCqwrC= NIPM[WFld3ewcnXneYxifGW|IFXSJIV5eHKnc4Ppc44hcW6mdXPl[EBjgSB2LV;IMXQhyqB? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB2OUOxOkc,OjJyNEmzNVY9N2F-
MCF-7 M{T6cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TtbFHDqM7:TR?= MlXrOU0yOCCm M1;iTpN2eHC{ZYPz[ZMhTTJvaX7keYNm\CCpcn;3eIghcW6qaXLpeIlwdg>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB2OUOxOkc,OjJyNEmzNVY9N2F-
HCC-1428 NXrXS41kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PDPVHDqM7:TR?= NEjVbJM2NTFyIHS= M4flW5N2eHC{ZYPz[ZMhTTJvaX7keYNm\CCpcn;3eIghcW6qaXLpeIlwdg>? M2PsSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MES5N|E3Lz5{MkC0PVMyPjxxYU6=
MDA-361 NG\lZ45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLpNeKh|ryP M{f6[|UuOTBiZB?= Mkeyd5VxeHKnc4Pld{BGOi2rbnT1Z4VlKGe{b4f0bEBqdmirYnn0bY9v NYjkd5ZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOFk{OTZpPkKyNFQ6OzF4PD;hQi=>
ZR75-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDmdFJTOcLizszN MnTLOU0yOCCm NVHnNmFZe3WycILld5NmeyCHMj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w NFHKSHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC0PVMyPid-MkKwOFk{OTZ:L3G+
MCF-7/AC-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;nNE0xNjJizszN NVT1ZnNWPiCm M{f1eolvcGmkaYTzJINmdGxiZ4Lve5RpKG2xZHXzeIx6 NXfCdVFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwOFI4QTJpPkKyNFQzPzl{PD;hQi=>
MCF7 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHIS49SOTBiwsXN Mn73OFghcA>? MXXpcoR2[2W|IHPlcIwhcW6qaXLpeIlwdiC5aHnjbEBk[W5iYnWg[Y5p[W6lZXSgZpkh\mmkcn;icIF{fHN? NGnBOJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC0NVg5Pyd-MkKwOFE5QDd:L3G+
MMQ NFKxboNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\h[3kxNTZ{NTDuUS=> NF;yV5M4OiCq Ml3QdJJw\HWlZYOgd5RifGm|dHnjZYxtgSC|aXfubYZq[2GwdDDpcohq[mm2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdsLi MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjBzNUGwNUc,OjJyMUWxNFE9N2F-
MMQ MYnGeY5kfGmxbjDBd5NigQ>? MWCwMVYzPSCwTR?= NVKzUZB5PzJiaB?= MlrpdJJw\HWlZYOgZUB{fGG2aYP0bYNidGy7IIPp[45q\mmlYX70MEBld3OnLXTldIVv\GWwdDDy[YR2[3Srb36gbY4hWFKOIIPlZ5JmfGmxbh?= NVnQdIdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwNVUyODFpPkKyNFE2OTBzPD;hQi=>
MCF7 NIPTSWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DRXFAuOSEQvF2= MXiyOE0yOjBiaB?= M4S5W4lvcGmkaYTzJJRp\SCpcn;3eIghd2ZiTVPGO{1[Si1zwrC= NFXZV489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi2N|I2QCd-MkG4OlMzPTh:L3G+
HepG2 NF3sR4hCeG:ydH;zbZMhSXO|YYm= NV6xW4FmOC5zwrFOwG0> M3\3NFI1KGh? MV7hZo9tcXOqZYOgeIhmKGW|dILv[4VvNWmwZIXj[YQhfXBvcnXneYxifGmxbjDv[kBieG:DSTDhcoQh[XCxTR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThzNkKzN{c,OjF6MU[yN|M9N2F-
MCF7–iFR3 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvvc5p4OjBvMUCwJI5O MnLaPVYhcA>? NVvR[mNb\W6qYX7j[ZMhSVBvaX7keYNm\CClZXzsJIdzd3e2aDDpcohq[mm2aX;u M1LnRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{myPFg6Lz5{MUe5Nlg5QTxxYU6=
MCF7S NYrWbmJZTnWwY4Tpc44hSXO|YYm= NIrYWGwyyqEQvF2= MWW0PEBp NVfvd|FH\G:5boLl[5Vt[XSnczDveoVz[WyuIFXS{tEheHKxdHXpckBt\X[nbIO= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV|M{G5OUc,OjF3M{OxPVU9N2F-
MCF7 M{\1OWZ2dmO2aX;uJGF{e2G7 NU\uSZF{OcLizszN NF;VO3M1QCCq MkPH[I94dnKnZ4XsZZRmeyCxdnXyZYxtKEWUzsGgdJJwfGWrbjDs[ZZmdHN? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV|M{G5OUc,OjF3M{OxPVU9N2F-
MCF7S MWXGeY5kfGmxbjDBd5NigQ>? NVHZVpRROcLizszN MYW3JIQ> NHrXeJJifHSnboXheIV{KHS3bX;yd5Bp\XKnIH\vdo1ifGmxbjDhcoQheHKxbHnm[ZJifGmxbh?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV|M{G5OUc,OjF3M{OxPVU9N2F-
MCF7S MkiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TCWlAvPS9zIN88US=> NIT6O2E4KGR? M3;CT2ROW09? M1SxcoRm[3KnYYPld{Bk\WyuIHX4dIFve2mxbh?= M2nzPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUOzNVk2Lz5{MUWzN|E6PTxxYU6=
T47D  MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\jU4Y1KG6P NGL6Omk1OCCq NHHOe|J{fXCycnXzd4V{KGOnbHyg[5Jwf3SqIH3v[IVz[XSnbIm= M1PoUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEiwN|kyLz5{MUS4NFM6OTxxYU6=
BT474  MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX20JI5O NF7R[2E1OCCq Mo\Hd5VxeHKnc4Pld{Bk\WyuIHfyc5d1cCCvb3TldoF1\Wy7 M1KxblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEiwN|kyLz5{MUS4NFM6OTxxYU6=
T47D  NUG1UlU2TnWwY4Tpc44hSXO|YYm= M3\ublExKG6P MYO0NEBp NGOzcnlld3ewcnXneYxifGW|IFXS{tEheHKxdHXpci=> NYi1O5dGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0PFA{QTFpPkKxOFgxOzlzPD;hQi=>
BT474  Mk\YSpVv[3Srb36gRZN{[Xl? NVnEb5RxOTBibl2= NWHvVmxtPDBiaB?= MVHkc5dvemWpdXzheIV{KEWUzsGgdJJwfGWrbh?= M13XV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEiwN|kyLz5{MUS4NFM6OTxxYU6=
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MCF7 MULGeY5kfGmxbjDhd5NigQ>? NHzFZXUyOCC3TR?= NYDn[HBtOyCmYYnz MWfJcohq[mm2aX;uJI9nKGmwY4LlZZNmKGmwIIDyc4xq\mW{YYTpc44hd2ZiZYP0do9o\W5icnXj[ZB1d3JiZYjwdoV{e2mwZzDNR2Y4KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEOg[IF6ew>? MoTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{N{WzNVUoRjF5Mke1N|E2RC:jPh?=
MVLN NGX4fGZHfW6ldHnvckBie3OjeR?= Mkj2NlQhcHK| M1v1cmFkfGm4aYT5JIF1KEWUIHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oIHXzeJJw\2WwIILld5BwdnOnIHXs[Y1mdnRvZILpeoVvKGenbnWgeJJidnOjY4TpeoF1cW:wIHnuJG1XVE5iY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? M2j2RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Mke1N|E2Lz5zN{K3OVMyPTxxYU6=
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MCF7 NFuyb3RHfW6ldHnvckBie3OjeR?= M{Wxd|Q2KG2rboO= NEnlUGJDcW6maX7nJIFn\mmwaYT5JJRwKGW|dILv[4VvKHKnY3XweI9zKGGucHjhJIlvKGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcO0ifRUKgZYNkfW23bHH0bY9vKGGodHXyJFQ2KG2rboOgZpkhf2ixbHWgZ4VtdCClb33w[ZRqfGmxbjDibY5lcW6pIHHzd4F6 NHjmSos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGzN|c4Pyd-MUmxN|M4Pzd:L3G+
MCF7 NH;iOWhHfW6ldHnvckBie3OjeR?= M{nQPVI1KGi{cx?= NUXhb3JkTG:5bjDy[Yd2dGG2aX;uJI9nKGW|dILv[4VvKHKnY3XweI9zKGGucHjhJIV5eHKnc4Ppc44hcW5iaIXtZY4hVUOINzDj[YxteyCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF|M{e3O{c,OTlzM{O3O|c9N2F-
MCF7 M4nTbGZ2dmO2aX;uJIF{e2G7 Mn;YNVAxKG6P NGfTRYszPCCqcoO= MV;Ec5dvKHKnZ4XsZZRqd25ib3[g[ZN1em:pZX6gdoVk\XC2b4KgZYxxcGFiZYjwdoV{e2mxbjDpckBpfW2jbjDNR2Y4KGOnbHzzJIF1KDFyMDDuUUBi\nSncjCyOEBpenNiYomgbY1ufW6xZnz1c5Jme2OnbnPlJJN1[WmwaX7n NWq5dnBHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxN|M4PzdpPkG5NVM{Pzd5PD;hQi=>
MCF7:D5L MoXjSpVv[3Srb36gZZN{[Xl? M1fKe|EhfU1? Mnz2RY51[WexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGmwIHj1cYFvKE2FRke6SFVNKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gSXJGNWSncHXu[IVvfCCudXPp[oVz[XOnIHX4dJJme3Orb36gZZQhOSC3TR?= Mn7rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NEWzPFYoRjF7OES1N|g3RC:jPh?=
HEK293 Ml7DSpVv[3Srb36gZZN{[Xl? NEnV[pYyKHWP M1;qNWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiRWLi[ZRiKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGVTTS2mZYDlcoRmdnRibIXjbYZmemG|ZTDlfJBz\XO|aX;uJIF1KDFidV2= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh2NUO4Okc,OTl6NEWzPFY9N2F-
Ishikawa NFS5cJZHfW6ldHnvckBie3OjeR?= M{LtelEhfU1? MnnkRY51[WexbnnzeEBi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGVTTS2mZYDlcoRmdnRiYXzrZYxqdmVicHjvd5Bp[XSjc3WgcIV3\Wy|IHH0JFEhfU1? NVXRWpFiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4OFU{QDZpPkG5PFQ2Ozh4PD;hQi=>
HeLa MWLGeY5kfGmxbjDhd5NigQ>? M{H4e|EhfU1? NHfSfJo1QCCqcoO= M1f5bmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hTVKjbIDoZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWOYTDj[YxteyClb3X4dJJme3OrbnegSXJGNUVzYj3MeYMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBme3S{YXTpc4wucW6mdXPl[EB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBifCBzIIXNJIFnfGW{IES4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> NX[0TWhFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NlE1QTJpPkKwOlIyPDl{PD;hQi=>
HeLa MoHzSpVv[3Srb36gZZN{[Xl? MXKxNFAhdk1? MnnxNlAhfG9iMkSgbJJ{ MojLRY51[WexbnnzeEBi[3Srdnn0fUBifCCOQlSgc4YhTVKjbIDoZUBz\WOncITvdkBqdiCqdX3hckBJ\UyjIHPlcIx{KGG|c3Xzd4VlKGG|IHLsc4Ns[WSnIH;mJHNTSy1zIHLpcoRqdmdidH:gdoVk\XC2b4KgZZQhOTByIH7NJIFnfGW{IEKwJJRwKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= M2fYflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NES4N|Q3Lz5{M{S0PFM1PjxxYU6=
MCF7 MXnGeY5kfGmxbjDhd5NigQ>? M4HafFExKG6P Moe1O|IhcHK| MlTOTY5pcWKrdHnvckBw\iBzN3LleIEu\XO2cnHkbY9tNWmwZIXj[YQhcW5iRWLhcJBp[SCyb4PpeIl3\SCqdX3hckBOS0Z5IHPlcIx{KHC{b3zp[oVz[XSrb36gZZN{\XO|ZXSgZZMhYzOKXT30bJlucWSrbnWgbY5kd3Kyb4LheIlwdiCjdDCxNEBvVSCjZoTldkA4OiCqcoOgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRqdmd? NY\PRZFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFg3PTJpPkK0PVA5PjV{PD;hQi=>
MCF7 M2rxT2Z2dmO2aX;uJIF{e2G7 NETY[|kyKHWP NX7WeVdVOjBiaILz NYrHb3diUW6mdXP0bY9vKG:oIHXzeJJw\2WwIILlZ4VxfG:{LXHsdIhiKGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3DSlch[2WubIOgZZQhOSC3TTDh[pRmeiB{MDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MmG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{m0PFUoRjJ3OEe5OFg2RC:jPh?=
MCF7 NWC2XFRWTnWwY4Tpc44h[XO|YYm= NH7EfZExNjBzIITvJFMxOCCwTR?= NVzzOVlmPDhiaILz MWnE[Ydz[WSjdHnvckBi[3Srdnn0fUBw\iCHUnHsdIhiKHKnY3XweI9zKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYSgNE4xOSC2bzCzNFAhdk1iYX\0[ZIhPDhiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MoLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MEewNVIoRjJ4NEC3NFEzRC:jPh?=
Neuro2a M3rLPGZ2dmO2aX;uJIF{e2G7 M{XXUVEhfU1? M2[0fGlvcGmkaYTpc44hd2ZiRGKyOEBqdiCGaHPyO{1l\W[rY3nlcpQhdW:3c3WgUoV2em9{YTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckA4NUSKQzDs[ZZmdHNiYYSgNUB2VSCkeTDMR{1OWy:JQz3NV{BidmGueYPpdy=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd6OU[1O{c,OjZ5OEm2OVc9N2F-
MCF-7 M1fI[2Z2dmO2aX;uJIF{e2G7 NXjSVZVGOyCwTTygN|Ahdk1uIEOwNEBvVSxiMzD1US=> NET1TIwzPCCqcoO= MXjEc5dvemWpdXzheIlwdiCxZjDFVoFteGijIHnuJIh2dWGwIF3DSk04KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCxZjDld5Rz[WSrb3ytbY5lfWOnZDDHVmVDOSCvUl7BJIxmfmWuczDheEA{KHhiMUCnMVkhVSxiMzD4JFExLy16IF2sJFMhgCBzMDetO{BONCB|IIigNVAoNTZiTTDh[pRmeiB{NDDodpMh[nliUmStVGNTKG2ndHjv[C=> NY\B[WJ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVc{OjBpPkK4OlU4OzJyPD;hQi=>
MCF-7 MVLGeY5kfGmxbjDhd5NigQ>? MlT0N{BvVSxiM{Cgcm0tKDNyMDDuUUwhOyC3TR?= M3XweVI1KGi{cx?= MmTmSI94dnKnZ4XsZZRqd25ib3[gSXJidHCqYTDpckBpfW2jbjDNR2YuPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[g[ZN1emGmaX;sMYlv\HWlZXSgVIdTKG2UTlGgcIV3\Wy|IHH0JFMhgCBzMDetPUBONCB|IIigNVAoNThiTTygN{B5KDFyJz23JG0tKDNieDCxNEcuPiCPIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3IhdWW2aH;k MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3N{OyNEc,Ojh4NUezNlA9N2F-
MCF-7 NWLQT4J7TnWwY4Tpc44h[XO|YYm= M2HYeFMhdk1uIEOwJI5ONCB|MECgcm0tKDNidV2= NWHEOldFOjRiaILz MX\Ec5dvemWpdXzheIlwdiCxZjDFVoFteGijIHnuJIh2dWGwIF3DSk04KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCxZjDld5Rz[WSrb3ytbY5lfWOnZDDwV|IhdVKQQTDs[ZZmdHNiYYSgN{B5KDFyJz25JG0tKDNieDCxNEcuQCCPLDCzJJghOTBpLUegUUwhOyC6IEGwK{03KE1iYX\0[ZIhOjRiaILzJIJ6KFKWLWDDVkBu\XSqb3S= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3N{OyNEc,Ojh4NUezNlA9N2F-
MCF7 Mn7HSpVv[3Srb36gZZN{[Xl? MWqxNEB2VQ>? NH63eFEzPCCqcoO= NFPtTHdKdmS3Y4Tpc44hd2Zic3Xs[YN1cX[nIHXzeJJw\2WwIILlZ4VxfG:{IHHsdIhiKGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3DSlch[2WubIOgZZQhOTBidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NYrsTGM4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OlI4OzdpPkK5OVYzPzN5PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MDM2 / ER; 

PubMed: 24747123     


MCF7 (A) and T47D (B) cells were cultured in the presence of different concentrations of fulvestrant (Fulv) for 66 h. ER and MDM2 were detected by Western blot and normalized to β-actin. The decrease in protein expression (shown relative to vehicle control treatment) after fulvestrant treatment was calculated for each drug concentration. *P < 0.05 compared to vehicle control treatment. 

p-ErbB3 Y1289 / ErbB3 / p-ErbB4 Y1056 / ErbB4 / p-AKT / AKT / p-ERK / ERK; 

PubMed: 21396094     


Western blots showing the effect of increasing concentrations of heregulin β1 (HRGβ1) (0.1 to 10 ng/ml for five minutes) on total and phosphorylated ErbB3, ErbB4, AKT and extracellular signal-regulated kinases 1 and 2 (ERK1/2) protein expression in MCF-7 and T47D cells, maintained for 7 days in the presence of either fulvestrant (100 nM) of vehicle control (ethanol). 

ErbB3; 

PubMed: 23999432     


Immunofluorescence of ErbB3 in cells cultured for 24 hours with or without fulvestrant. 

ERα; 

PubMed: 19815064     


Breast cancer cells were treated with different doses of ICI for 3 hours. The expression levels of ERα and actin were assayed by Western blot using anti-ERα and anti-actin antibodies.

24747123 21396094 23999432 19815064
Immunofluorescence
IGF-1R / Erα; 

PubMed: 19815064     


Confocal microscope study of fulvestrant-induced ERα and IGF-1R co-localization on the cell membrane in non-permeabilized condition. Cells were starved in 1% DCC medium for 24 hr and then treated with either vehicle (left) or ICI at 100 nM (right) for 15 min. Treated cells were fixed in 4% formaldehyde and stained with mouse monoclonal anti-IGF-1R antibody (a), rabbit polyclonal anti-ERα antibody (b), or nuclear staining with DAPI (c). The merged images are shown in d. Scale bar = 20 μm.

Snail / E-cadherin ; 

PubMed: 23936773     


E-cadherin and Snail protein localization in MCF-7 cells 48 h after treatment of cells with E2 and the tested agents. C: control (untreated cells); E2: cells treated with 17β-estradiol; Fulv: cells treated with E2 + 100 nM Fulv. The image is representative of three independent experiments using a magnification of 63X.

19815064 23936773
Growth inhibition assay
Cell viability; 

PubMed: 29787591     


MTT assay of MCF-7 cells treated with various concentration of fulvestrant. Statistically significant reduced the % viability in MCF-7 cells (One-way ANOVA; ****p < 0.0001).

29787591
In vivo Fulvestrant is devoid of uterotropic activity, and when co-administered with estradiol, it effectively blocks the uterotropic action of estradiol with ED50 of 0.06 mg/kg/day s.c. in immature female rats. A single s.c. injection of 5 mg of Fulvestrant suspension blocks completely the growth of MCF-7 xenografts. The growth of transplants of the BrlO human breast tumor is also suppressed effectively by 10 μM Fulvestrant. [1] Fulvestrant (10 mg/rat, s.c.) reduces the androgen receptor expression, ERK1/2 phosphorylation and cell proliferation in the rat ventral prostate. [7] Fulvestrant also displays anti-angiogenesis in the chick egg chorioallantoic membrane. [8]

Protocol

Cell Research:[1]
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  • Cell lines: MCF-7 breast cancer cells
  • Concentrations: 2.9 nM
  • Incubation Time: 5 days
  • Method: MCF-7 cells are cultured in multiwell plates (24-well, seeding density 4 × 104) in minimal essential medium containing phenol red, insulin (10 μg/mL), and 5% charcoal-stripped fetal calf serum without additional estradiol. Fulvestrant and/or estradiol are added in fresh medium 2 days after seeding. Cultures are maintained for 5 days with one further medium change and growth is assessed by measurement of total cell protein at the beginning and end of treatment and compared with that of controls treated with ethanol (0.1%) alone.
    (Only for Reference)
Animal Research:[6]
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  • Animal Models: The human breast cancer xenografts MCF-7 in nude mice
  • Dosages: 5 mg/mouse
  • Administration: s.c. injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (164.8 mM)
Water Insoluble
Ethanol ''100 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
0.4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 606.77
Formula

C32H47F5O3S

CAS No. 129453-61-8
Storage powder
in solvent
Synonyms ZD 9238, ZM 182780
Smiles CC12CCC3C(C1CCC2O)C(CC4=C3C=CC(=C4)O)CCCCCCCCCS(=O)CCCC(C(F)(F)F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04762979 Recruiting Drug: Alpelisib|Drug: Fulvestrant|Drug: Aromatase inhibitor Hormone Receptor Positive Breast Carcinoma|HER2-negative Breast Cancer|PIK3CA Mutant Metastatic Breast Cancer Amanda Parkes|Novartis|University of Wisconsin Madison|Big Ten Cancer Research Consortium February 12 2021 Phase 2
NCT04567420 Recruiting Drug: Palbociclib|Drug: Fulvestrant|Drug: Adjuvant Therapy Breast Cancer Criterium Inc. February 9 2021 Phase 2
NCT04544189 Recruiting Drug: Alpelisib|Drug: Fulvestrant|Drug: Placebo Breast Neoplasms Novartis Pharmaceuticals|Novartis January 20 2021 Phase 2
NCT04524000 Recruiting Drug: Alpelisib|Drug: Fulvestrant Advanced Breast Cancer Novartis Pharmaceuticals|Novartis November 20 2020 Phase 2
NCT04483505 Recruiting Drug: Combination Rogaratinib + palbociclib + fulvestrant Breast Cancer Metastatic|Hormone Receptor Positive Malignant Neoplasm of Breast Fundacion CRIS de Investigación para Vencer el Cáncer|Bayer|Pfizer|Apices Soluciones S.L. November 25 2020 Phase 1
NCT04572295 Recruiting Drug: E7090|Drug: Fulvestrant|Drug: Exemestane Breast Neoplasms Eisai Co. Ltd.|Eisai Inc. October 9 2020 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Is there any information for the half-life of fulvestrant (Cat No.S1191)?

  • Answer:

    S1191, is about 13.5 to 18.5 hours in vivo: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2750771/. The half life of these compounds in cell culture might be different and we generally recommend replenishing with fresh drug every 24-48 hours.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID