Afatinib (BIBW2992)

Catalog No.S1011

For research use only.

Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFRwt, EGFR L858R , EGFR L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively. Afatinib induces autophagy.

Afatinib (BIBW2992) Chemical Structure

CAS No. 850140-72-6

Selleck's Afatinib (BIBW2992) has been cited by 304 publications

Purity & Quality Control

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Biological Activity

Description Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFRwt, EGFR L858R , EGFR L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively. Afatinib induces autophagy.
Targets
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
ErbB4 [2]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 1 nM 10 nM 14 nM
In vitro

BIBW2992 shows potent activity against both wild-type and mutant forms of EGFR and HER2. It is similar to Gefitinib in potency against L858R EGFR, but about 100-fold more active against the Gefitinib resistant L858R-T790M EGFR double mutant. BIBW2992 exhibits potent effects on both EGFR and HER2 phosphorylation in vivo. It compares favorably to reference compounds (such as Lapatinib et al.) in all cell types tested, such as human epidermoid carcinoma cell line A431 expressing wt EGFR, murine NIH-3T3 cells transfected with wt HER2, as well as breast cancer cell line BT-474 and gastric cancer cell line NCI-N87, which express endogenous HER2. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 MnL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTBwMEC1O|Ih|ryP M3rvTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
KASUMI-1 M125XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvNTWM2OD1yLkCwPVEyKM7:TR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
HCC2218 MljIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTBwMEC5PFch|ryP M1jKWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
KARPAS-45 Mk\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMEG2O|Eh|ryP NGG4UHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
HSC-4 MkPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7MTGhKSzVyPUCuNFE4PDhizszN M3fjSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
CHL-1 NEDyUHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjjRZUxUUN3ME2wMlAyQDN7IN88US=> M1fvTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
KY821 M3TVT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfZbZFKSzVyPUCuNFIxPiEQvF2= MkK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
NCI-N87 NELzdIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTaTWM2OD1yLkCyO|c{KM7:TR?= NFSzOVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
DOK NHXWdlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwMEOxPVQh|ryP MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
DSH1 NV\PVJR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnQSGxtUUN3ME2wMlA{OzF2IN88US=> Mm\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
MDA-MB-175-VII MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWr4O4dJUUN3ME2wMlA{PDF4IN88US=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H2170 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIr0XXJKSzVyPUCuNFM4OjhizszN MkfMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
BT-474 MlrBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HBfGlEPTB;MD6wOFk5PiEQvF2= Mmi5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
EFO-27 MkXtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTBwMEWxPFIh|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H720 NGrIfYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnQTWM2OD1yLkC1N|I2KM7:TR?= NWfmVGpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
PC-14 NHrwSldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrFTWM2OD1yLkC1OlY5KM7:TR?= M4r2[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
EC-GI-10 MoL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFn6c4FKSzVyPUCuNFU6OTJizszN MmizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
KYSE-450 MkSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfBUWZvUUN3ME2wMlA2QTd6IN88US=> MmrSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
BPH-1 M{L5NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXMXI1PUUN3ME2wMlA2QTh6IN88US=> M3u2XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
NCI-H292 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXOTWM2OD1yLkC2NFkh|ryP MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
H9 M2fFfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrYdmlHUUN3ME2wMlA4OTN{IN88US=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H526 NYG2dmU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTBwMEe4NlMh|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H1623 MnjTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTBwMEiwNlUh|ryP NEHzSJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
KYSE-140 M13NOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHveXh6UUN3ME2wMlA5OzNizszN M4m4SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
Ca9-22 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnCTJg3UUN3ME2wMlA5OzV|IN88US=> NYLZe4ttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
AU565 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fMPWlEPTB;MD6wPFUxPCEQvF2= NV7Udo15RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
ZR-75-30 NWf2N25yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\vN5ZXUUN3ME2wMlA6PTR3IN88US=> NFSzUlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
NCI-H1770 NXvYSoZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTBwMUCyPEDPxE1? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
LB2241-RCC NWjB[opHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGe5W4hKSzVyPUCuNVA5QDRizszN NUDkXlhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
CAL-39 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnWdJhKSzVyPUCuNVE4QTJizszN NH;MSHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
UACC-893 M2Tycmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUX5cZg4UUN3ME2wMlEyQDh3IN88US=> M2rPUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
LU-65 NX\ab5NRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DKTWlEPTB;MD6xNlY{OyEQvF2= NIS3eJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
HN MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWWzZWxpUUN3ME2wMlE{ODd5IN88US=> M4eyd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
TI-73 M2DFXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLaTWM2OD1yLkG0O|E2KM7:TR?= M4\VNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
KU812 M3O0[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITWdndKSzVyPUCuNVU1PTZizszN NWOxcVZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
SW13 M2jDd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3xV3JoUUN3ME2wMlE3OjF4IN88US=> MlK4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
BB30-HNC MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonRTWM2OD1yLkG2OVc3KM7:TR?= M{\pb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
HSC-2 NW\WVWNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUS0PGk3UUN3ME2wMlE3QDN5IN88US=> M17ue|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
BV-173 MoDyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1G3dmlEPTB;MD6xO|U6PiEQvF2= MmPsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
CAL-27 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYq0OmR3UUN3ME2wMlE4PjN4IN88US=> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
SBC-1 M3HUd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwMUi5OVkh|ryP NWf1dmF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
CAL-33 Ml3aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17nSmlEPTB;MD6yNFE5PCEQvF2= MmX5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
NCI-H209 M17ke2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXyc3lKSzVyPUCuNlA1PiEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
COR-L105 MkLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;sTWM2OD1yLkKxNFg1KM7:TR?= M1XKOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
HSC-3 NV;MPIQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTBwMkGxPVgh|ryP NHr0Zpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
NCI-H69 Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DnRmlEPTB;MD6yNVQ2OSEQvF2= NVPEeZA3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
MOLT-13 M4[4bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMkO5O|Uh|ryP NEXJ[Is9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
ML-2 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwMkS4NFIh|ryP NEXJbW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
IGROV-1 NVS2SY5sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3qTWM2OD1yLkK1PVUzKM7:TR?= M4rQ[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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EoL-1-cell MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWOx[VhCUUN3ME2wMlI3QDl{IN88US=> NH7nRXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
HO-1-N-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTBwMke4OVMh|ryP NVPXU49lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
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DoTc2-4510 M4izUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfXZnMzUUN3ME2wMlMzOjF7IN88US=> Mn;GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
BB65-RCC NH:xSmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXwRXhxUUN3ME2wMlM1PTl|IN88US=> M3XkZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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A388 NYDMSnpIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\BVmFZUUN3ME2wMlM3QDV5IN88US=> Ml3RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
NUGC-3 NYLSc2gxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3T5NGlEPTB;MD6zPFM4OyEQvF2= M{XNTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
KYSE-180 NYn5[GhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXoR2VxUUN3ME2wMlM6ODh7IN88US=> NWXxVI1JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
TE-6 NYnwWJBQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\DTWM2OD1yLkSwNlQ3KM7:TR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
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NCI-H1648 M4nlSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTBwNESxPFIh|ryP NWLSTVBXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
DU-145 NFrxWFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnX6TWM2OD1yLkS0PFM{KM7:TR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
CW-2 MkjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwNE[5NVQh|ryP NHPtb4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
A431 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mof5TWM2OD1yLkS4NlM{KM7:TR?= NFPrXWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
SAS M4nRO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XCeWlEPTB;MD60PFM6QCEQvF2= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H2126 MonuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTBwNUG5NlYh|ryP NWLTVW1IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
NCI-H358 M2TL[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXMTWM2OD1yLkWzNlEh|ryP MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
FADU M{D0VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPmfmZKSzVyPUCuOVQ1ODRizszN NH\SXJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
ACN MnXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojKTWM2OD1yLkW1NFk1KM7:TR?= M2W0XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
HCC70 Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVi5SFNoUUN3ME2wMlU4PjZzIN88US=> NI\obJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
NCI-H1838 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHuWWxKSzVyPUCuOlAzQTlizszN MnXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
CAL-54 MojES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETIWnpKSzVyPUCuOlE3OjFizszN NIO1cpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
NCI-H1975 M3LuU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHRTWM2OD1yLk[xO|I5KM7:TR?= M3X2PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
COR-L88 M2f6Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2C5cWlEPTB;MD62OFEyOyEQvF2= NEW3[Io9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
CTV-1 NH7Vc|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVG4OWFLUUN3ME2wMlY1Ozl{IN88US=> MlmyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
EW-18 NET6fWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzqTWM2OD1yLk[4Olk5KM7:TR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
HCC1806 MlLTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrPfJdIUUN3ME2wMlY6PjZ4IN88US=> NETKZXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
HuP-T4 NVfkfnhyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTBwNkm3OkDPxE1? M1n6elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
MDA-MB-361 MmnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTBwN{K2OlIh|ryP M{K4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
NCI-H1573 MofUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\sWZdKSzVyPUCuO|M3OyEQvF2= NFXaTIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
KU-19-19 MoTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTBwN{e0OlQh|ryP NXvtVVhZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
COLO-678 NWfHfFZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTBwOEC4N|Qh|ryP M3:2UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
C-33-A M1XmWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rDRmlEPTB;MD64NVI6PyEQvF2= NXz2UG57RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
Calu-3 NYLtRVhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTBwOEG0N|Qh|ryP M1LxPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
AGS M1f3R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LGeWlEPTB;MD64N|Y1QCEQvF2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
SCC-25 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7BTWM2OD1yLki0NVcyKM7:TR?= NYL5SXliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
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SW1710 NFXsRoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{[3WWlEPTB;OD6xPFUyPSEQvF2= MmizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
D-502MG NGizUVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3mw[2lEPTB;OD6yPVI5OiEQvF2= MmrXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
NCI-H1993 M1WwZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXXN|FQUUN3ME24MlM{PjNzIN88US=> NGH0cIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
SCC-4 NIS4bXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjKTWM2OD16LkS0NFU6KM7:TR?= M121c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
HAL-01 M4\FU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo[1TWM2OD16LkWxPVEh|ryP M13LTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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OCI-AML2 NIXvNYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\j[WlEPTB;OD65OVQ5OSEQvF2= M3TafFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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BALL-1 NED1Z4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DT[WlEPTB;OT6yNVkxPyEQvF2= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
SF539 M{j0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPsS49xUUN3ME25MlI{PTZ{IN88US=> NHHYNGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
CAMA-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTlwMkixNlgh|ryP NWLm[|JTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
C32 M2m2bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXke4NIUUN3ME25MlM{ODZ2IN88US=> M3fmdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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NCI-H1563 M3;EfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrzVZhWUUN3ME25Mlc3ODNzIN88US=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
CAL-85-1 MkDTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTlwOEezPFMh|ryP NYfCVIk1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
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DEL NUCye4UyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13NR2lEPTB;MUCuN|k1PiEQvF2= MonEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
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OVCAR-4 NVvTW|NzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHaeVhKSzVyPUGwMlg5OzVizszN MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
CAL-72 NYnaN4E{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjYTWM2OD1zMD65N|kyKM7:TR?= NIX3OVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
HCC1954 NYC1UY1{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTFzLkC3OVQh|ryP NX3OWoZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
EFO-21 M{DCc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mny5TWM2OD1zMT6xNFQ2KM7:TR?= NGX0No49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
FTC-133 MkLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nYUGlEPTB;MUGuNVQxPyEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
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UACC-62 NX33O2tiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjJboxiUUN3ME2xNU4zOzZ6IN88US=> M2\RblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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ALL-PO MonGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXlTWM2OD1zMT6yOVEzKM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
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SW626 M3zPfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVi1W4U1UUN3ME2xNU42PzV3IN88US=> NEXmSWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
RPMI-2650 NXLzO5R4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnUXGZKSzVyPUGxMlU5QDVizszN NYDxb|V2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5M{[1OU8oRlODTlfFVlww[T5?
MC-IXC M1;PR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvjWIVKSzVyPUGxMlY4OjhizszN NGnSeFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
HOP-62 M1\Jd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7hd3liUUN3ME2xNU43PzZ2IN88US=> NFzjPZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
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M14 NWLtOGJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXaTWM2OD12ND6xN|MzKM7:TR?= Mk\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
COLO-680N NHv6TpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILPcHdKSzVyPUS0MlM{QSEQvF2= M4r3O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzN4NUWvK|5USU6JRWK8M4E,
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EW-1 NHPXZ4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfSTWM2OD12NT65Olg6KM7:TR?= NIi3d|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
SW948 MlfwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\Ze2dKSzVyPUS2MlQ1PjJizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H460 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3WwWGlEPTB;NE[uOVI{KM7:TR?= MlLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
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TGBC11TKB NY[1Z2FiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[2emVKSzVyPUS3MlE4PzdizszN MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
HD-MY-Z NWr3PIRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\KOZlKSzVyPUS4Mlc5PiEQvF2= Ml7lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
NCI-H226 M2jpXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV22c3Y{UUN3ME20PU44Pzh3IN88US=> NHuz[HY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
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LB2518-MEL MmnoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoX2TWM2OD12OT64O|I{KM7:TR?= MkHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OzZ3NT:nQnNCVkeHUkyvZV4>
A101D MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnqcHllUUN3ME20PU46PDJ3IN88US=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVc{PjV3Lze+V2FPT0WUPD;hQi=>
NCI-H2087 NED3OFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;wRWlEPTB;NEmuPVY5OiEQvF2= NGXUcWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTd|NkW1M{c,W0GQR1XSQE9iRg>?
Assay
Methods Test Index PMID
Western blot CIP2A / p-AKT / AKT / PARP ; p-EGFR / p-IGF1R / p-ERK / p-AKT ; p-VEGFR2 / VEGFR2 / JAG1 / DLL4 / NICD1 25537503 26052929 28533486
Growth inhibition assay Cell apoptosis ; Cell viability 25537503 26515464
Immunofluorescence p65 ; vacuoles 26317651 29713246
In vivo Daily oral administration of BIBW2992 at 20 mg/kg for 25 days results in dramatic tumor regression with a cumulative treated/control tumor volume ratio (T/C ratio) of 2%. Reduced phosphorylation of EGFR and AKT is confirmed by immunohistochemical staining of tissue sections. Therefore, like lapatinib and neratinib, BIBW2992 is a next generation tyrosine kinase inhibitor (TKI) that inhibits human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) kinases irreversibly. BIBW2992 is not only active against EGFR mutations targeted by first generation TKIs like Erlotinib or Gefitinib, but also against those insensitive to these standard therapies. [1]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro kinase activity assays:

    The wild type tyrosine kinase domain of the human EGFR as well as that of the EGFR L858R/T790M double mutant are fused to Glutathione-S-transferase (GST), and extracted. Enzyme activity is then assayed in the presence of the inhibitor BIBW2992, serially diluted in 50% DMSO. A random polymer pEY (4:1) is used as substrate and biotinylated pEY (bio-pEY) is added as a tracer substrate. The kinase domain of HER2 is cloned using the baculovirus system and extracted similarly to that of EGFR kinase. Details of assays for EGFR, HER2, SRC, BIRK and VEGFR2 kinase activity are available in Supplementary information.

Cell Research:

[1]

  • Cell lines: NSCLC cells
  • Concentrations: 0-10 μM
  • Incubation Time: 1 hour
  • Method:

    1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: Athymic NMRI-nu/nu female mice
  • Dosages: 20 mg/kg
  • Administration: Oral administration
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 97 mg/mL
(199.61 mM)
Ethanol 15 mg/mL
(30.86 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 485.94
Formula

C24H25ClFN5O3

CAS No. 850140-72-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN(C)CC=CC(=O)NC1=C(C=C2C(=C1)C(=NC=N2)NC3=CC(=C(C=C3)F)Cl)OC4CCOC4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04795245 Active not recruiting Drug: afatinib Non-squamous Non-Small Cell Lung Cancer Boehringer Ingelheim August 26 2021 --
NCT04497584 Recruiting Drug: Afatinib + Prednisone Advanced Squamous Non Small Cell Lung Cancer University of Texas Southwestern Medical Center August 4 2021 Phase 2
NCT04413201 Recruiting Drug: Afatinib|Drug: Osimertinib Non-squamous NSCLC Michael Hopp|Boehringer Ingelheim|Johannes Gutenberg University Mainz September 11 2020 Phase 4
NCT04206787 Recruiting Drug: Afatinib Non-squamous Non-Small Cell Lung Cancer Boehringer Ingelheim May 20 2020 --
NCT04158947 Recruiting Drug: Afatinib|Drug: T-DM1 HER2-positive Breast Cancer|Brain Metastases xuexin he|Second Affiliated Hospital School of Medicine Zhejiang University May 10 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
What’s the recommendation about reconstitution of the compound for in vivo animal study?

Answer:
For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

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