Afatinib (BIBW2992)

Catalog No.S1011

Afatinib (BIBW2992) Chemical Structure

Molecular Weight(MW): 485.94

Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFRwt, EGFR L858R , EGFR L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively.

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In DMSO USD 240 In stock
USD 120 In stock
USD 210 In stock
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Cited by 37 Publications

4 Customer Reviews

  • Cytoxicity of irreversible EGFR TKIS and ethacrynic acid on breast cells lines. A, Mean IC50 value of Afatinib.

    Oncotarget, 2016, 7(36):58038-58050. Afatinib (BIBW2992) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

  • Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of BIBW2992 for 3h,followed by 15-minute stimolation of 100ng/ml EGF

    Dr. Zhang of Tianjin Medical University. Afatinib (BIBW2992) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Afatinib (BIBW2992) inhibits EGFR/ErbB irreversibly in vitro with IC50 of 0.5, 0.4, 10, 14, 1 nM for EGFRwt, EGFR L858R , EGFR L858R/T790M ErbB2 (HER2) and ErbB4 (HER4), respectively.
Targets
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
ErbB4 [2]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 1 nM 10 nM 14 nM
In vitro

BIBW2992 shows potent activity against both wild-type and mutant forms of EGFR and HER2. It is similar to Gefitinib in potency against L858R EGFR, but about 100-fold more active against the Gefitinib resistant L858R-T790M EGFR double mutant. BIBW2992 exhibits potent effects on both EGFR and HER2 phosphorylation in vivo. It compares favorably to reference compounds (such as Lapatinib et al.) in all cell types tested, such as human epidermoid carcinoma cell line A431 expressing wt EGFR, murine NIH-3T3 cells transfected with wt HER2, as well as breast cancer cell line BT-474 and gastric cancer cell line NCI-N87, which express endogenous HER2. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 NF\rcFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4iw[2lEPTB;MD6wNFU4OiEQvF2= M3LmbHNCVkeHUh?=
KASUMI-1 NYf6O3BoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzpOlVKSzVyPUCuNFA6OTFizszN MlrGV2FPT0WU
HCC2218 MmXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDHWXpqUUN3ME2wMlAxQTh5IN88US=> M4HkTnNCVkeHUh?=
KARPAS-45 M4HGZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUO2TmU{UUN3ME2wMlAyPjdzIN88US=> MV;TRW5ITVJ?
HSC-4 NWXiTpVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXHe3loUUN3ME2wMlAyPzR6IN88US=> M1nHbnNCVkeHUh?=
CHL-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTBwMEG4N|kh|ryP M{[1O3NCVkeHUh?=
KY821 NFnBT3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTBwMEKwOkDPxE1? NVjNZVBSW0GQR1XS
NCI-N87 MmXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTBwMEK3O|Mh|ryP NXjtVIhxW0GQR1XS
DOK MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIn3N5RKSzVyPUCuNFMyQTRizszN MnXnV2FPT0WU
DSH1 NXXzfZpMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7vZoNKSzVyPUCuNFM{OTRizszN NU\hT|R7W0GQR1XS
MDA-MB-175-VII NEPtb3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfheYlKSzVyPUCuNFM1OTZizszN Mn20V2FPT0WU
NCI-H2170 M2nFe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2Gyd2lEPTB;MD6wN|czQCEQvF2= NFj5OmRUSU6JRWK=
BT-474 NWOwPFF[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2j4SmlEPTB;MD6wOFk5PiEQvF2= M4DRfXNCVkeHUh?=
EFO-27 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3QTWM2OD1yLkC1NVgzKM7:TR?= MkDHV2FPT0WU
NCI-H720 M3:5Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmf1TWM2OD1yLkC1N|I2KM7:TR?= NWLoNFZTW0GQR1XS
PC-14 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwMEW2Olgh|ryP M2HvV3NCVkeHUh?=
EC-GI-10 NXrRfWxpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXmxcYYzUUN3ME2wMlA2QTF{IN88US=> M3eyVXNCVkeHUh?=
KYSE-450 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrYTYFDUUN3ME2wMlA2QTd6IN88US=> M3vYZnNCVkeHUh?=
BPH-1 NFTFXHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;JXmlEPTB;MD6wOVk5QCEQvF2= NFG0[WJUSU6JRWK=
NCI-H292 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTBwME[wPUDPxE1? NY\ZTXU1W0GQR1XS
H9 MkW1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXOb3hKSzVyPUCuNFcyOzJizszN MnnHV2FPT0WU
NCI-H526 NYrFeohTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTBwMEe4NlMh|ryP NWnVUJM4W0GQR1XS
NCI-H1623 M2HmV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XscGlEPTB;MD6wPFAzPSEQvF2= M1XLO3NCVkeHUh?=
KYSE-140 M3v2bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYSye3lpUUN3ME2wMlA5OzNizszN M4TvcnNCVkeHUh?=
Ca9-22 NF\5cFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwMEizOVMh|ryP NIDPNJRUSU6JRWK=
AU565 NUTJdnhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4ftVmlEPTB;MD6wPFUxPCEQvF2= MnPhV2FPT0WU
ZR-75-30 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7oTWM2OD1yLkC5OVQ2KM7:TR?= NWHzfZoxW0GQR1XS
NCI-H1770 M3XNdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXlVWNyUUN3ME2wMlExOjhizszN M1T6TXNCVkeHUh?=
LB2241-RCC M17xfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYD1WGlrUUN3ME2wMlExQDh2IN88US=> NVuyOJZmW0GQR1XS
CAL-39 Mn7GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTJTWM2OD1yLkGxO|kzKM7:TR?= M1ntdXNCVkeHUh?=
UACC-893 MnTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHvSWhKSzVyPUCuNVE5QDVizszN NVjYNHFIW0GQR1XS
LU-65 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrCfIxKSzVyPUCuNVI3OzNizszN NH:yUpdUSU6JRWK=
HN NH3nSWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zpOmlEPTB;MD6xN|A4PyEQvF2= NX:yXlF2W0GQR1XS
TI-73 NFvjcHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonKTWM2OD1yLkG0O|E2KM7:TR?= MoXjV2FPT0WU
KU812 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\ieoJKSzVyPUCuNVU1PTZizszN NXXobGQ3W0GQR1XS
SW13 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTBwMU[yNVYh|ryP M33zcXNCVkeHUh?=
BB30-HNC NVixbpV1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnhR242UUN3ME2wMlE3PTd4IN88US=> MmD2V2FPT0WU
HSC-2 Mnj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXpNI9KSzVyPUCuNVY5OzdizszN MlW1V2FPT0WU
BV-173 NUXmeJQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwMUe1PVYh|ryP NYK4eVhCW0GQR1XS
CAL-27 NYDJNnl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknNTWM2OD1yLkG3OlM3KM7:TR?= M{nRXHNCVkeHUh?=
SBC-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwMUi5OVkh|ryP MWXTRW5ITVJ?
CAL-33 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmP0TWM2OD1yLkKwNVg1KM7:TR?= M4jmO3NCVkeHUh?=
NCI-H209 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;6c3hKSzVyPUCuNlA1PiEQvF2= MWXTRW5ITVJ?
COR-L105 NF;pbGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn2VldoUUN3ME2wMlIyODh2IN88US=> MnPYV2FPT0WU
HSC-3 M3\ReGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTDN406UUN3ME2wMlIyOTl6IN88US=> NIWy[nNUSU6JRWK=
NCI-H69 MkDxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;lTWM2OD1yLkKxOFUyKM7:TR?= MnPUV2FPT0WU
MOLT-13 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjzPIs3UUN3ME2wMlI{QTd3IN88US=> NUftZldSW0GQR1XS
ML-2 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3ZVZRKSzVyPUCuNlQ5ODJizszN M3nke3NCVkeHUh?=
IGROV-1 MkHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTBwMkW5OVIh|ryP MnrEV2FPT0WU
TK10 NUHSS2I6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV33Rnk4UUN3ME2wMlI3PzN6IN88US=> MWrTRW5ITVJ?
EoL-1-cell NEf4RVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnPfYVKSzVyPUCuNlY5QTJizszN M4XnVHNCVkeHUh?=
HO-1-N-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fZcWlEPTB;MD6yO|g2OyEQvF2= MUjTRW5ITVJ?
BHY NVTUfoc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\3WGFKSzVyPUCuNlg3PTVizszN NX3STZhyW0GQR1XS
LU-139 NIDzTpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTBwM{C1NUDPxE1? NWTq[3FqW0GQR1XS
23132-87 MlfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3WySGlEPTB;MD6zNVkzOyEQvF2= MnLtV2FPT0WU
ACHN MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTBwM{G5PVQh|ryP M{PpU3NCVkeHUh?=
DoTc2-4510 NFPLeYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3S[2RDUUN3ME2wMlMzOjF7IN88US=> M1HHd3NCVkeHUh?=
BB65-RCC NHfnZ2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnWemZKSzVyPUCuN|Q2QTNizszN MoTtV2FPT0WU
HLE MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwM{WwOFYh|ryP NUXDVIpkW0GQR1XS
HCC1419 NH3udG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH:wTIxKSzVyPUCuN|Y1ODhizszN NYXGOnJLW0GQR1XS
A388 M4PMWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPCS3ByUUN3ME2wMlM3QDV5IN88US=> Mnv0V2FPT0WU
NUGC-3 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPKTWM2OD1yLkO4N|c{KM7:TR?= MUnTRW5ITVJ?
KYSE-180 MoD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LnTWlEPTB;MD6zPVA5QSEQvF2= M3nJdnNCVkeHUh?=
TE-6 M4XX[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVj5dHBbUUN3ME2wMlQxOjR4IN88US=> MoXoV2FPT0WU
A498 M3TyPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTBwNEC2O|Qh|ryP MlP2V2FPT0WU
NCI-H1648 NEHDdGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4H3emlEPTB;MD60OFE5OiEQvF2= MnvrV2FPT0WU
DU-145 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3X1e2lEPTB;MD60OFg{OyEQvF2= M3X3WXNCVkeHUh?=
CW-2 NXTPb2txT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXG5T2tGUUN3ME2wMlQ3QTF2IN88US=> NELzSGpUSU6JRWK=
A431 NX3uW2ZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnviTWM2OD1yLkS4NlM{KM7:TR?= Ml;jV2FPT0WU
SAS M1zYN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHT0VYZKSzVyPUCuOFg{QThizszN MX3TRW5ITVJ?
NCI-H2126 MoDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrmTpBKSzVyPUCuOVE6OjZizszN NF\PT3lUSU6JRWK=
NCI-H358 NXn6UXJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3y2[mlEPTB;MD61N|IyKM7:TR?= MWXTRW5ITVJ?
FADU MmrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4frb2lEPTB;MD61OFQxPCEQvF2= M2XRU3NCVkeHUh?=
ACN MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLuVpdKSzVyPUCuOVUxQTRizszN NVzPSIZYW0GQR1XS
HCC70 NU[1bJNoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nLd2lEPTB;MD61O|Y3OSEQvF2= M3\zUXNCVkeHUh?=
NCI-H1838 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTBwNkCyPVkh|ryP M4\1XnNCVkeHUh?=
CAL-54 NX70ZnoyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{n4dmlEPTB;MD62NVYzOSEQvF2= NGixepFUSU6JRWK=
NCI-H1975 M1TxVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELOfJJKSzVyPUCuOlE4OjhizszN MnrLV2FPT0WU
COR-L88 MoPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3wdJVyUUN3ME2wMlY1OTF|IN88US=> Mk\2V2FPT0WU
CTV-1 MkKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTBwNkSzPVIh|ryP MWDTRW5ITVJ?
EW-18 NV20Npl4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFf6T|dKSzVyPUCuOlg3QThizszN Mnv4V2FPT0WU
HCC1806 NH3jSllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTBwNkm2OlYh|ryP M1zh[HNCVkeHUh?=
HuP-T4 NU[yUXNqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjzVoNKUUN3ME2wMlY6PzZizszN MX\TRW5ITVJ?
MDA-MB-361 NX75WpF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTBwN{K2OlIh|ryP NH3yNHNUSU6JRWK=
NCI-H1573 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3pTWM2OD1yLkezOlMh|ryP NUPhUGlLW0GQR1XS
KU-19-19 M1W3Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTBwN{e0OlQh|ryP NFr6[GdUSU6JRWK=
COLO-678 MkPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTBwOEC4N|Qh|ryP MmPzV2FPT0WU
C-33-A NHvnfXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTBwOEGyPVch|ryP NF\LcFJUSU6JRWK=
Calu-3 NIDLVlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTBwOEG0N|Qh|ryP NXXGS3p1W0GQR1XS
AGS MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfWTWM2OD1yLkizOlQ5KM7:TR?= M{\uUHNCVkeHUh?=
SCC-25 NUDpd4R2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\yPYZKSzVyPUCuPFQyPzFizszN NVv1eZh[W0GQR1XS
JVM-2 NHjPfnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLFPHp1UUN3ME2wMlg5Ojl7IN88US=> M1jmN3NCVkeHUh?=
HCE-T M2mxNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml72TWM2OD1yLki4OFI5KM7:TR?= MX7TRW5ITVJ?
ES4 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPMTWM2OD1yLkm3PFM3KM7:TR?= NIGzTGlUSU6JRWK=
EKVX Mnj6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTFwMEGwOlQh|ryP NX\ZSGhCW0GQR1XS
D-542MG M{DZ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGW5Z5FKSzVyPUGuNFExPjRizszN NIL4UmNUSU6JRWK=
SW1116 NWfSco05T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXVR5RKSzVyPUGuNFEzQDdizszN MU\TRW5ITVJ?
5637 MmnZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{e1NGlEPTB;MT6wNVUyKM7:TR?= NX3GbFZrW0GQR1XS
HT-1376 NV;WcopYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILEcnRKSzVyPUGuNFE2OTNizszN MnG2V2FPT0WU
NB69 NXHod4hDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETGTmpKSzVyPUGuNFI6PDVizszN NEn6WpFUSU6JRWK=
YKG-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYToe5J5UUN3ME2xMlA2PDF7IN88US=> NX\uOHllW0GQR1XS
EW-3 NHTxOHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzOTWM2OD1zLkC3PFg{KM7:TR?= MX7TRW5ITVJ?
GCIY NEHyT4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTFwMEixNFQh|ryP NWXSe2ZrW0GQR1XS
SK-MES-1 M33oUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG[3Z49KSzVyPUGuNVE1QDdizszN NXf2e5ZCW0GQR1XS
PANC-08-13 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEP5UnFKSzVyPUGuNVY1OTZizszN Mm\KV2FPT0WU
SK-NEP-1 M4TPUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nK[WlEPTB;MT6xO|UyOyEQvF2= M{LTWXNCVkeHUh?=
SW954 MonPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTFwMkCyNVQh|ryP NFu0[5lUSU6JRWK=
NCI-SNU-5 NFz3XlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjNbndKSzVyPUGuNlM6KM7:TR?= MXvTRW5ITVJ?
SW48 NFvvXJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW[2dlhrUUN3ME2xMlI2PDN{IN88US=> MnTaV2FPT0WU
HT-3 NUGyW|RIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTFwMki3OVMh|ryP NWnPdpRlW0GQR1XS
ETK-1 NXjaW5RoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzG[W9iUUN3ME2xMlM4PDV4IN88US=> M3jhN3NCVkeHUh?=
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KURAMOCHI NFq2TIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{P2d2lEPTB;Nz65OFgyOSEQvF2= NF7sV2dUSU6JRWK=
BE-13 NU\FNVlKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTdwOUixNVIh|ryP M1jrVHNCVkeHUh?=
VMRC-RCZ MkDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRThwMEG2PVch|ryP NXXiOJZGW0GQR1XS
NCI-H727 M4H4Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonDTWM2OD16LkGwOFIh|ryP M1nFbHNCVkeHUh?=
SW1710 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRThwMUi1NVUh|ryP MYfTRW5ITVJ?
D-502MG NWn6ZoVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fKN2lEPTB;OD6yPVI5OiEQvF2= NFjSZWNUSU6JRWK=
NCI-H1993 Mlu4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjKSnFKSzVyPUiuN|M3OzFizszN NVL0eWlHW0GQR1XS
SCC-4 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRThwNESwOVkh|ryP MU\TRW5ITVJ?
HAL-01 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljwTWM2OD16LkWxPVEh|ryP M1PJWnNCVkeHUh?=
GT3TKB M{PXZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTITYIxUUN3ME24MlY4QTZ2IN88US=> MoG1V2FPT0WU
SK-OV-3 Mo\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vSeGlEPTB;OD63NVMzQSEQvF2= MYrTRW5ITVJ?
COLO-829 MonjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7UOVBKSzVyPUiuPFA6PzZizszN NG[wVIRUSU6JRWK=
OCI-AML2 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk[4TWM2OD16Lkm1OFgyKM7:TR?= MXrTRW5ITVJ?
OS-RC-2 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrwbFk2UUN3ME25MlAzOTlizszN NF7WRVFUSU6JRWK=
BFTC-909 Mo\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrITWM2OD17LkC2O|k6KM7:TR?= M4jQbnNCVkeHUh?=
EW-11 MkTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDETWM2OD17LkG3OlU{KM7:TR?= NEDv[IhUSU6JRWK=
HCE-4 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;mbJJUUUN3ME25MlIyPTV6IN88US=> MnTOV2FPT0WU
BALL-1 MkPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XaXGlEPTB;OT6yNVkxPyEQvF2= MVrTRW5ITVJ?
SF539 MlLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTzXG16UUN3ME25MlI{PTZ{IN88US=> NFG1UnBUSU6JRWK=
CAMA-1 NVzVepFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLiTWM2OD17LkK4NVI5KM7:TR?= NWPCd2ZWW0GQR1XS
C32 NU\ONXpuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPhTWM2OD17LkOzNFY1KM7:TR?= M4PMTnNCVkeHUh?=
NCI-H1703 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXtTWM2OD17LkO0N|c2KM7:TR?= M4\xXnNCVkeHUh?=
NCI-H1650 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LrWmlEPTB;OT62NFk1PiEQvF2= M2nRZ3NCVkeHUh?=
NCI-H1563 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUO2fnRvUUN3ME25Mlc3ODNzIN88US=> MnW5V2FPT0WU
CAL-85-1 MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfOTWM2OD17Lki3N|g{KM7:TR?= M4jNWnNCVkeHUh?=
UM-UC-3 NV\oWnhXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjFVYtKSzVyPUmuPVUxOyEQvF2= NFXXS4VUSU6JRWK=
BxPC-3 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIqyWJFKSzVyPUGwMlA6PzJizszN MnHHV2FPT0WU
MHH-PREB-1 NEPqTppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2PnPGlEPTB;MUCuNVk6QSEQvF2= NHe2[3BUSU6JRWK=
DEL NYHFU4l[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXWTWM2OD1zMD6zPVQ3KM7:TR?= Mn7HV2FPT0WU
HuCCT1 M1rxUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HSTGlEPTB;MUCuOlA{OyEQvF2= NXjCN45EW0GQR1XS
OVCAR-4 NUTPSo57T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHldnQzUUN3ME2xNE45QDN3IN88US=> NG\j[|NUSU6JRWK=
CAL-72 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DQZWlEPTB;MUCuPVM6OSEQvF2= NE\EPVBUSU6JRWK=
HCC1954 NEnCWWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPkbYI{UUN3ME2xNU4xPzV2IN88US=> NFzyflBUSU6JRWK=
EFO-21 NF7ZSIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHn5TndKSzVyPUGxMlExPDVizszN NYm3cXQ1W0GQR1XS
FTC-133 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTFzLkG0NFch|ryP MV3TRW5ITVJ?
SK-MEL-24 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfE[|E5UUN3ME2xNU4zOzZ4IN88US=> M321O3NCVkeHUh?=
UACC-62 NUH1bZZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmT2TWM2OD1zMT6yN|Y5KM7:TR?= NFfvOYVUSU6JRWK=
NCI-H650 Mn;IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvQNWZKSzVyPUGxMlI1OThizszN M1;jd3NCVkeHUh?=
ALL-PO M16xXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELqeFdKSzVyPUGxMlI2OTJizszN M3zGZ3NCVkeHUh?=
T84 NX7LWoJMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPlTWM2OD1zMT6zPVA4KM7:TR?= NGrZb5FUSU6JRWK=
IST-MEL1 NFLzdmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrtV5lKSzVyPUGxMlQ2PTlizszN NH\ySVZUSU6JRWK=
SW626 NIToXIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvmTWM2OD1zMT61O|U2KM7:TR?= NEHlbplUSU6JRWK=
RPMI-2650 MmHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjm[5FYUUN3ME2xNU42QDh3IN88US=> NV\T[JpnW0GQR1XS
MC-IXC NH7qbmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX:0dJo6UUN3ME2xNU43PzJ6IN88US=> NFTlVpRUSU6JRWK=
HOP-62 Mmm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonXTWM2OD1zMT62O|Y1KM7:TR?= M{TvPHNCVkeHUh?=
NOS-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHPTFI3UUN3ME2xNU44OTB7IN88US=> MX\TRW5ITVJ?
SW1573 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTETWM2OD1zMT63Nlk2KM7:TR?= M{DNSHNCVkeHUh?=
WM-115 MkjPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTFzLke1PFgh|ryP M2LpTXNCVkeHUh?=
HuO9 M2PJe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVqyS5JrUUN3ME2xNU45Pzd2IN88US=> NFTiZmFUSU6JRWK=
YAPC NYDwOnJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmThTWM2OD1zMT65NlU6KM7:TR?= M4DDSHNCVkeHUh?=
MDA-MB-453 NHe1[|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LzNGlEPTB;MUGuPVUyPSEQvF2= NUDp[ohtW0GQR1XS
NCI-H596 MkjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvFTWM2OD1zMj6wN|Y6KM7:TR?= MmjCV2FPT0WU
A172 NIP4fZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTmN3NKSzVyPUGyMlA4QThizszN NIq5R3lUSU6JRWK=
RS4-11 NV\lSmYxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfsflNKSzVyPUGyMlE4OjVizszN M2\Ge3NCVkeHUh?=
HCC1937 M1zjeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTF{Lke1OlQh|ryP NYnXb5U4W0GQR1XS
SW837 NYK4V4h[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3ETWM2OD1zMj65NFI5KM7:TR?= MV\TRW5ITVJ?
NMC-G1 NH;VWIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXHTWM2OD1zMj65NlM1KM7:TR?= NFjhcIpUSU6JRWK=
PFSK-1 NIrlZXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\LTWM2OD1zMz6wOkDPxE1? MYrTRW5ITVJ?
DBTRG-05MG MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{HOVmlEPTB;MUOuNFY{OiEQvF2= NE\DS3lUSU6JRWK=
SK-UT-1 M2qyWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTF|LkGwPFMh|ryP M1jqUHNCVkeHUh?=
OVCAR-5 NEHpSYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfQUpJKSzVyPUGzMlE2QTNizszN NGHU[YtUSU6JRWK=
8505C NXi1cnliT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTF|LkKwOlch|ryP NGrqV3ZUSU6JRWK=
SK-MEL-1 NYq3UW96T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDDbJlKSzVyPUGzMlcyOzVizszN MkHsV2FPT0WU
LK-2 M4DPeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFixc4FKSzVyPUGzMlc3OzlizszN MYDTRW5ITVJ?
OE33 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPtUI9mUUN3ME2xN{44QDZizszN NH;yU4xUSU6JRWK=
Ramos-2G6-4C10 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPkTWM2OD1zND6wNlAzKM7:TR?= M4TEbXNCVkeHUh?=
EW-13 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fsTGlEPTB;MUSuNFM2OiEQvF2= NFu0V2tUSU6JRWK=
NCI-H1793 NEjtfG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYO1R2VQUUN3ME2xOE4zODh5IN88US=> M2n1VXNCVkeHUh?=
BHT-101 MlvvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonsTWM2OD1zND6yOFEh|ryP MmnBV2FPT0WU
RKO MmS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TuU2lEPTB;MUSuOVA4PSEQvF2= MX;TRW5ITVJ?
NEC8 MlH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTF2LkW0O|Ih|ryP NVrTbIJ6W0GQR1XS
PANC-10-05 NFXae4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\KfFlZUUN3ME2xOE43OjR5IN88US=> MoT5V2FPT0WU
NCI-H2030 M2nTeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzTZoZKSzVyPUG0MlY1PDhizszN MorKV2FPT0WU
C2BBe1 M2DjZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1K4XGlEPTB;MUSuO|Y1KM7:TR?= M1e3SHNCVkeHUh?=
EM-2 Mn7MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPLR45KSzVyPUG0Mlg2PzZizszN Mon4V2FPT0WU
HCC1395 NWrsfpFJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTF2LkmzN|gh|ryP M1\3RnNCVkeHUh?=
A2058 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTF3LkC0NFkh|ryP MnHJV2FPT0WU
SW1463 Ml31S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVG3TI1DUUN3ME2xOU4xPDh3IN88US=> NYX6ZWFoW0GQR1XS
J82 NYraNYRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnOxTWM2OD1zNT6wOVch|ryP M360THNCVkeHUh?=
CAL-120 NInuUZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETSV5NKSzVyPUG1MlE1PDdizszN M1f4OHNCVkeHUh?=
COLO-824 NW\Rb3NGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLHWlFKSzVyPUG1MlE2PzlizszN MVvTRW5ITVJ?
LNCaP-Clone-FGC Mn3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\4WlA5UUN3ME2xOU41OjR6IN88US=> Ml3WV2FPT0WU
CP66-MEL M{S2UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELtcmhKSzVyPUG1MlQ3QDhizszN MVLTRW5ITVJ?
OC-314 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTF3LkS3NVQh|ryP MmfiV2FPT0WU
OVCAR-8 NGfRbpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmi5TWM2OD1zNT64PFYyKM7:TR?= MXPTRW5ITVJ?
MFE-280 MlTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnaXoFKSzVyPUG2MlA1QDhizszN NW\MV4VzW0GQR1XS
MHH-NB-11 NFXYbZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTF4LkC4N|kh|ryP NX3zb3h7W0GQR1XS
SW1990 Ml;XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHhOog{UUN3ME2xOk4yPzV3IN88US=> NXzMdWNTW0GQR1XS
U-266 NYS5eFJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTETWM2OD1zNj6yNlk6KM7:TR?= M{C0PXNCVkeHUh?=
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L-363 NV7sdI4{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mom3TWM2OD1zNj60OVc4KM7:TR?= M2TvNHNCVkeHUh?=
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SK-MEL-3 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPVSpV2UUN3ME2xOk41Pzh5IN88US=> M4\4VnNCVkeHUh?=
HH NYLVUFFIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTBTWM2OD1zNj61NVYzKM7:TR?= M4T3SXNCVkeHUh?=
no-10 NUHUTZV[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3T3[2lEPTB;MU[uOlM1OyEQvF2= NUjEbVc5W0GQR1XS
HuP-T3 Ml3HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXuweohpUUN3ME2xOk43PTd4IN88US=> M3jMZXNCVkeHUh?=
QIMR-WIL NWO2[VVJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXkRoI5UUN3ME2xOk44OTB3IN88US=> MVLTRW5ITVJ?
NCI-H1092 M1vQTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXV[JdqUUN3ME2xOk45OzF4IN88US=> NIjOR4NUSU6JRWK=
MZ2-MEL MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfjRVRKSzVyPUG2Mlg1ODNizszN NHjRfHhUSU6JRWK=
ES3 NYTGTHgxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\vW|A{UUN3ME2xOk46OTN2IN88US=> MV7TRW5ITVJ?
COLO-684 MorJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfGZpg5UUN3ME2xOk46PDd5IN88US=> Ml7yV2FPT0WU
NCI-H661 M{LlVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGO5[YdKSzVyPUG2Mlk3QTRizszN NILVcIpUSU6JRWK=
CP50-MEL-B MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnWdVY1UUN3ME2xO{4xOzR|IN88US=> Mnn4V2FPT0WU
NCI-H2405 NF;uOnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfGSHpKSzVyPUG3MlE6PzRizszN NFXUPIpUSU6JRWK=
U-2-OS MonoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHkVVhKSzVyPUG3MlU{ODdizszN NFnPdFFUSU6JRWK=
RERF-LC-MS NIjuOFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\Demo4UUN3ME2xO{42PTB6IN88US=> NFHERlRUSU6JRWK=
NCI-H2342 NF\pR5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3L3V2lEPTB;MUeuOVU4KM7:TR?= MWLTRW5ITVJ?
ES8 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DjPWlEPTB;MUeuOlA5PyEQvF2= NVfZRppMW0GQR1XS
MOLT-16 NULtd3lmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTF5Lki5N|Qh|ryP M4\yZ3NCVkeHUh?=
RH-18 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXBTWM2OD1zOD6wNFM2KM7:TR?= MofKV2FPT0WU
D-283MED NWnKTWlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHpTWM2OD1zOD6wNFg5KM7:TR?= NVvDSZQ{W0GQR1XS
RCC10RGB NWLmRmI5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnDTWM2OD1zOD6wOVU6KM7:TR?= NV7QSG1FW0GQR1XS
NCI-H1437 NF7SdVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTF6LkG2OFEh|ryP M4DGU3NCVkeHUh?=
SW982 NGPkZWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPR[nFKSzVyPUG4MlI3PzFizszN MonVV2FPT0WU
IST-MES1 NUPDR2NNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLUZVhwUUN3ME2xPE41PDJ6IN88US=> NXTWSlRGW0GQR1XS
A704 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fFXGlEPTB;MUiuOFc2OyEQvF2= M2ewbXNCVkeHUh?=
ESS-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTF6LkWyNFUh|ryP NI\oVI5USU6JRWK=
SCH MnK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYP1XVBGUUN3ME2xPE43PDV4IN88US=> M{naZnNCVkeHUh?=
COLO-320-HSR MlzuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzySJhKSzVyPUG4Mlc1PiEQvF2= NXvOeXZDW0GQR1XS
Mo-T M130eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4WySWlEPTB;MUiuPFIxPCEQvF2= MWHTRW5ITVJ?
Saos-2 NVmxVlJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zVWGlEPTB;MUmuNVE3OiEQvF2= MX3TRW5ITVJ?
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NCI-H2122 M{jFRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnmW|NxUUN3ME2zOE4{PDdzIN88US=> NUHkdJlHW0GQR1XS
ABC-1 M1zBc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPiTWM2OD1|ND6zOlk{KM7:TR?= Mnz4V2FPT0WU
NCI-H1355 MnXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmX6TWM2OD1|ND6zPFg6KM7:TR?= NGDqWWdUSU6JRWK=
NOMO-1 M1jGfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\ve2lEPTB;M{SuOVcxOyEQvF2= MXTTRW5ITVJ?
MZ7-mel NWL5R5pUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7XTWM2OD1|ND62OFg4KM7:TR?= NGLTdm1USU6JRWK=
NCI-H810 NVXsenpGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nSd2lEPTB;M{SuO|I4QCEQvF2= M2XNS3NCVkeHUh?=
D-392MG Ml\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLiTWM2OD1|NT6wOVY2KM7:TR?= NWrj[I1vW0GQR1XS
LB771-HNC MkDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mln0TWM2OD1|NT6xOlU5KM7:TR?= MVnTRW5ITVJ?
COLO-679 NXe5bodST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTN3LkO4PEDPxE1? NWO3PWhkW0GQR1XS
SK-PN-DW MoDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFixeVdKSzVyPUO1MlQ{PjlizszN MXLTRW5ITVJ?
VA-ES-BJ M{izW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XkO2lEPTB;M{WuOFY1QSEQvF2= MYLTRW5ITVJ?
KNS-42 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDnTWM2OD1|NT61PFI3KM7:TR?= MorSV2FPT0WU
RVH-421 NV3nOFNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTN3Lke2OFUh|ryP M2LQenNCVkeHUh?=
P30-OHK MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUj5T4xbUUN3ME2zOU45PzV{IN88US=> NXHEVHV{W0GQR1XS
G-402 NGjUWGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXwOlZKSzVyPUO1Mlk5PjdizszN MnPCV2FPT0WU
S-117 M1zZeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjNeZNKSzVyPUO2MlMxODhizszN M2PtXHNCVkeHUh?=
RXF393 NYXIZpNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7tflBiUUN3ME2zOk4{Pzh3IN88US=> NEi3TXlUSU6JRWK=
LCLC-103H Mnr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPDcpRKUUN3ME2zOk44QDB|IN88US=> NI\ycmNUSU6JRWK=
U-118-MG NXfleoZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHUS|FKSzVyPUO2Mlg5PDdizszN NELGT4pUSU6JRWK=
8-MG-BA MmfZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHZbolKSzVyPUO2Mlk1QDNizszN NWjj[JBNW0GQR1XS
MFH-ino Mn;VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTN5LkOzO|Eh|ryP MmnYV2FPT0WU
NCI-H23 NY\OSFM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTN5LkSxPVYh|ryP NYrGfpM{W0GQR1XS
NCI-H446 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2L5UWlEPTB;M{euOFg3OiEQvF2= MmjDV2FPT0WU
LU-99A M1;hNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTN5LkW3N|Eh|ryP NYnEfohOW0GQR1XS
KP-N-YN NWfudpVUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHpNlVzUUN3ME2zO{43OTl3IN88US=> MkDaV2FPT0WU
SCC-9 NFLzSpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInQUWVKSzVyPUO3Mlc5OzhizszN MonpV2FPT0WU
EFM-19 M{nFS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;UOmlEPTB;M{euO|k3PyEQvF2= Mmr5V2FPT0WU
A427 M2HkRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjBO4dCUUN3ME2zO{46OTB5IN88US=> MmHFV2FPT0WU
LB831-BLC MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojnTWM2OD1|OD62NFM6KM7:TR?= NULGXFc1W0GQR1XS
HOS MlPIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPZfVJYUUN3ME2zPU43Ojd|IN88US=> NV\jN4FzW0GQR1XS
LAN-6 NFz0PHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rJXWlEPTB;NECuNFEyOiEQvF2= NH7ESnNUSU6JRWK=
U251 M1;jXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknXTWM2OD12MD60NFU1KM7:TR?= MnvEV2FPT0WU
SNU-387 NIrOdZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\MTWM2OD12MD60OVU{KM7:TR?= NVrlXGNZW0GQR1XS
DMS-79 M3TQdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XFNWlEPTB;NECuOVg5QSEQvF2= M2XMW3NCVkeHUh?=
SK-LU-1 M{\FcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTRyLk[1Olgh|ryP M4G5OXNCVkeHUh?=
HT MnjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPFU4RKSzVyPUSwMlY3OzlizszN NYW4N|BNW0GQR1XS
KP-N-YS MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHO4XoFKSzVyPUSwMlY4PjhizszN NEjG[3NUSU6JRWK=
DMS-114 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFGwSJNKSzVyPUSwMlg{PzRizszN MWfTRW5ITVJ?
HUTU-80 NYP2UHhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVGyfWFnUUN3ME20NU4xPTZ7IN88US=> MknCV2FPT0WU
ONS-76 NWPFZVNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrHTWM2OD12MT60O|A6KM7:TR?= MXnTRW5ITVJ?
A673 NX3KR2MyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnBZYpKSzVyPUSxMlUyPTdizszN MnnQV2FPT0WU
HPAF-II NUi0eo14T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTRzLk[3NFkh|ryP MmPUV2FPT0WU
UACC-257 NFj6W41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXRTWM2OD12MT63N|Y6KM7:TR?= MYDTRW5ITVJ?
EW-22 NULZPVRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3PWXl5UUN3ME20NU45Pjl5IN88US=> NUTjd3NjW0GQR1XS
NCI-H1755 NHLBTZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvsTWM2OD12MT64O|I2KM7:TR?= MmTTV2FPT0WU
KYSE-150 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTR{LkG3PFIh|ryP MVrTRW5ITVJ?
CGTH-W-1 NXvqRm9PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTR{LkS5OVUh|ryP MmDTV2FPT0WU
NB13 NVXRbXpTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTR{Lk[yNVch|ryP NGHFOllUSU6JRWK=
SW872 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17Sc2lEPTB;NEKuOlM{OSEQvF2= NH:4e5ZUSU6JRWK=
KP-4 NFPNR3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\TTWM2OD12Mj64PFMzKM7:TR?= NVLxZ2xKW0GQR1XS
HCC38 NUXHZlVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTR{Lkm2NVgh|ryP NGfRNYhUSU6JRWK=
BB49-HNC NWe3U2JXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTR|LkKzOVYh|ryP NEH2fVZUSU6JRWK=
CAL-62 M4np[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTR|LkSwNlgh|ryP MUTTRW5ITVJ?
NCI-H2029 Mn3kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTR|LkW1PFEh|ryP M4LvbnNCVkeHUh?=
M14 NFKwcZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2C2VWlEPTB;NESuNVM{OiEQvF2= NIrwcIZUSU6JRWK=
COLO-680N M1XjPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTR2LkOzPUDPxE1? MV3TRW5ITVJ?
Hs-578-T NHK1[IhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTR3LkCyPVQh|ryP MX3TRW5ITVJ?
MSTO-211H NYfoR5V7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTR3Lkm0PFIh|ryP MYPTRW5ITVJ?
EW-1 NHqxeWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vJcWlEPTB;NEWuPVY5QSEQvF2= M{njfXNCVkeHUh?=
SW948 Mny0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIm5VHNKSzVyPUS2MlQ1PjJizszN NYCyOJMyW0GQR1XS
NCI-H460 NIDwUIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;2OXFKSzVyPUS2MlUzOyEQvF2= MojDV2FPT0WU
CAS-1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknaTWM2OD12Nj63O|AyKM7:TR?= NY\B[lE6W0GQR1XS
TGBC11TKB NXX3RlJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\hXlRSUUN3ME20O{4yPzd5IN88US=> MkPwV2FPT0WU
HD-MY-Z MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TaVmlEPTB;NEiuO|g3KM7:TR?= NFezZXNUSU6JRWK=
NCI-H226 NVy1eWtlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rkd2lEPTB;NEmuO|c5PSEQvF2= NUi4RVE4W0GQR1XS
PA-1 M2\lSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEG5VIhKSzVyPUS5Mlg{OzVizszN NEjQWolUSU6JRWK=
LB2518-MEL NFTFSVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4r1WmlEPTB;NEmuPFczOyEQvF2= M4fuV3NCVkeHUh?=
A101D NIHvUY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPlTWM2OD12OT65OFI2KM7:TR?= NF\tNGhUSU6JRWK=
NCI-H2087 NYDqbndkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXz2e|hkUUN3ME20PU46Pjh{IN88US=> NWn1SYVSW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Daily oral administration of BIBW2992 at 20 mg/kg for 25 days results in dramatic tumor regression with a cumulative treated/control tumor volume ratio (T/C ratio) of 2%. Reduced phosphorylation of EGFR and AKT is confirmed by immunohistochemical staining of tissue sections. Therefore, like lapatinib and neratinib, BIBW2992 is a next generation tyrosine kinase inhibitor (TKI) that inhibits human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) kinases irreversibly. BIBW2992 is not only active against EGFR mutations targeted by first generation TKIs like Erlotinib or Gefitinib, but also against those insensitive to these standard therapies. [1]

Protocol

Kinase Assay:

[1]

+ Expand

In vitro kinase activity assays:

The wild type tyrosine kinase domain of the human EGFR as well as that of the EGFR L858R/T790M double mutant are fused to Glutathione-S-transferase (GST), and extracted. Enzyme activity is then assayed in the presence of the inhibitor BIBW2992, serially diluted in 50% DMSO. A random polymer pEY (4:1) is used as substrate and biotinylated pEY (bio-pEY) is added as a tracer substrate. The kinase domain of HER2 is cloned using the baculovirus system and extracted similarly to that of EGFR kinase. Details of assays for EGFR, HER2, SRC, BIRK and VEGFR2 kinase activity are available in Supplementary information.
Cell Research:

[1]

+ Expand
  • Cell lines: NSCLC cells
  • Concentrations: 0-10 μM
  • Incubation Time: 1 hour
  • Method:

    1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Athymic NMRI-nu/nu female mice
  • Formulation: In 0.5% methocellulose-0.4% polysorbate-80 (Tween 80)
  • Dosages: 20 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 97 mg/mL (199.61 mM)
Ethanol 15 mg/mL (30.86 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 485.94
Formula

C24H25ClFN5O3

CAS No. 439081-18-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02780687 Recruiting Urologic Neoplasms Boehringer Ingelheim June 9 2016 Phase 2
NCT02131259 Completed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim May 7 2014 --
NCT02216617 Completed HNSCC Groupe Oncologie Radiotherapie Tete et Cou August 6 2014 Phase 1
NCT02451553 Recruiting Advanced Malignant Solid Neoplasm|Bile Duct Carcinoma|Recurrent Malignant Solid Neoplasm|Recurrent Pancreatic Carcinoma|Stage III Pancreatic Cancer AJCC v6 and v7|Stage IVA Pancreatic Cancer|Stage IVB Pancreatic Cancer University of Washington|National Cancer Institute (NCI) November 5 2015 Phase 1
NCT01523587 Completed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim March 5 2012 Phase 3
NCT01345682 Completed Head and Neck Neoplasms|Carcinoma Squamous Cell Boehringer Ingelheim January 5 2012 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID