Name: Protocatechuic acid
Brand: Selleck Chemicals
SKU: S3975
Availability: InStock
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Quality Control

Batch: S397501 DMSO]30 mg/mL]false]]]false]]]false Purity: 99.99%

Cited in Nature Medicine for its top-tier quality
COA
SDS
Datasheet

99.99

Related Targets	Bcl-2 Caspase PD-1/PD-L1 Ferroptosis p53 Synthetic Lethality STAT TNF-alpha Ras KRas
Other Apoptosis related Inhibitors	Importazole Genipin Pitstop 2 Plumbagin 6-Gingerol Crocin Colcemid Euphorbia factor L3 Sophoridine Pomolic acid

Solubility

In vitro
Batch:

DMSO : 30 mg/mL (194.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.5mg/ml (9.73mM)	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.21mg/ml (1.36mM)	Taking the 1 mL working solution as an example, add 50 μL of 4.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	154.12	Formula	C₇H₆O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	99-50-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	3,4-Dihydroxybenzoic acid, Protocatechuate			Smiles	C1=CC(=C(C=C1C(=O)O)O)O

Mechanism of Action

In vitro	Protocatechuic acid (PCA) inhibits cell migration and invasion at non-cytotoxic concentrations. It down-regulates the Ras/Akt/NF-κB pathway by targeting RhoB activation, which in turn leads to a reduction of MMP-mediated cellular events in cancer cells. In some cancer cells, PCA treatment suppresses cell adhesion and the production of interleukin (IL)-6, IL-8, vascular endothelial growth factor and the intercellular adhesion molecule-1, which would further attenuate angiogenic and metastatic actions. PCA will lower the expression of cleaved caspase-3 and decrease the rate of cardiomyocyte apoptosis induced by hypoxia/reoxygenation. PCA is proven to have the neuroprotective effects in PC12 cells.
In vivo	Protocatechuic acid (PCA) inhibits metastasis of B16/F10 melanoma cells to the liver in mice. PCA is seen to enhance learning and memory ability, and alleviate oxidative stress, apoptosis and glial proliferation following CIH exposure in rats. In addition, PCA administration also decreases the level of IL-1β in brain and increases the expression of BDNF and SYN. Its administration inhibits apoptosis by decreasing JNK/P38 phosphoryltion and the downstream target including c-fos, Bax and Cleaved Caspase-3 in the hippocampal and prefrontal cortex areas. PCA exerts significant neuroprotective effects in CIH rats.
References	[1] https://pubmed.ncbi.nlm.nih.gov/20840540/ [2] https://www.nature.com/articles/srep14507

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06396000	Not yet recruiting	Anesthesia; Adverse Effect	Saiqiang Guo\|Tongji Hospital	May 31 2024	--

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Protocatechuic acid Apoptosis related chemical

Chemical Structure

Molecular Weight: 154.12