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Aloperine Antiviral chemical

Name: Aloperine
Brand: Selleck Chemicals
SKU: S2420
Availability: InStock
Rating: 5 (8 reviews)

Cat.No.S2420

Aloperine is an isolated alkaloid in sophora plants such as Sophora alopecuroides L, and exhibits anti-inflammatory, antibacterial, antiviral, and anti-tumor properties.

Chemical Structure

Molecular Weight: 232.36

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal COVID-19 Parasite Reverse Transcriptase HIV HCV Protease
Other Antiviral Products	Moroxydine HCl GS-441524 Oleanolic Acid NGI-1 Lapachol Saikosaponin B2 Harringtonine U18666A Carrageenan Goitrin

Solubility

In vitro
Batch:

DMSO : 46 mg/mL (197.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 46 mg/mL

Water : 4 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.300mg/ml (9.90mM)	Taking the 1 mL working solution as an example, add 50 μL of 46 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.300mg/ml (9.90mM)	Taking the 1 mL working solution as an example, add 50 μL of 46 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	232.36	Formula	C₁₅H₂₄N₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	56293-29-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1CCN2CC3CC(C2C1)C=C4C3NCCC4

Mechanism of Action

In vitro	Compared with other alkaloids including sophoridine, sophocarpine, matrine, oxymatrine and cytisine, Aloperine treatment exerts the most potent cytotoxic activity against human cancer cell lines of differing tissue origins, including leukaemia cell lines HL-60, U937 and K562, oesophageal cancer EC109 cells, lung cancer A549 cells and the hepatocellular carcinoma HepG2 cell line with IC50 of 0.04 mM, 0.27 mM, 0.36 mM, 1.11 mM, 1.18 mM, and 1.36 mM, respectively. The strongest cytotoxic effect of this compound on HL-60 cells is observed at 72 hours with the inhibition rate of 94.1%. In contrast to the effect on leukaemia cells, up to 1 mM of this chemical does not significantly reduce the viability of normal PBMNCs at 72 hours. Treatment with this compound at 20 μM for 48 hours significantly induces apoptosis and autophagy in HL-60 cells in a dose-dependent manner.
In vivo	Topical application of 1% Aloperine suppresses 2, 4-dinitrofluorobenzene (DNFB)-induced increase in ear thickness and ear erythema in BALB/c mice, and significantly decreases the up-regulated mRNA and protein levels of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) induced by DNFB in ear biopsy homogenates. Topical application of this compound reduces the DNFB-induced dermatitis (dermatitis index and ear thickness) in NC/Nga mice at day 13 and day 14 in a dose-dependent manner. This compound treatment reduces DNFB-induced lymphocytes infiltration and eosinophils infiltration in a dose-dependent manner. This compound treatment also reduces the DNFB-induced infiltration of mast cells in a dose-dependent manner. This compound reduces the plasma level of IgE in a dose-dependent manner. This compound markedly reduces DNFB-induced increase in IL-4, IL-13 and IFN-γ productions, while it increases the IL-10 level in a dose-dependent manner. This compound treatment significantly reduces the cytokine levels of TNF-α, IL-1β and IL-6 in ear biopsies homegenates of NC/Nga mice in a dose-dependent manner.
References	[1] https://pubmed.ncbi.nlm.nih.gov/20006963/ [2] https://pubmed.ncbi.nlm.nih.gov/21159130/ [3] https://pubmed.ncbi.nlm.nih.gov/21371468/

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