ZZW-115

Catalog No.E1037

For research use only.

ZZW-115 is a potent NUPR1 inhibitor with IC50s of 0.42 μM in Hep2G cells and 7.75 μM in SaOS-2 cells. ZZW-115 shows evident antitumor activity through its interaction with NUPR1.

ZZW-115 Chemical Structure

CAS No. 10122-45-9

Purity & Quality Control

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Biological Activity

Description

ZZW-115 is a potent NUPR1 inhibitor with IC50s of 0.42 μM in Hep2G cells and 7.75 μM in SaOS-2 cells. ZZW-115 shows evident antitumor activity through its interaction with NUPR1.

Targets
NUPR1 [1]
(in Hep2G cells)
NUPR1 [1]
(in SaOS-2 cells)
0.42 μM 7.75 μM
In vitro

ZZW-115 is capable of efficiently killing the pancreatic tumor cell lines by targeting NUPR1 and inhibits the growth of other tumor-derived cells. ZZW-115 induced cell death is the consequence of a decrease of ATP production by the mitochondria accompanied by an increase of mitochondrial ROS production with a transient metabolic shift toward anaerobic glycolysis inhibiting.[1]

In vivo

ZZW-115 can disrupt tumor growth at low doses and can reduce tumor size for the xenografted tumors, without obvious neurological side effects in the mice. ZZW-115 is a very efficient anticancer drug candidate in vivo on mice because, due mainly to necrosis, it is capable not only of stopping tumor growth but also of decreasing the tumor size until its disappearance.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Panc-1 cells
  • Concentrations: 0 μM - 100 μM
  • Incubation Time: 24 h, 72 h
  • Method:

    Cells are plated in 96-well plates. Twenty-four hours later, the media are supplemented with 0–100 μM concentration of ZZW-115, and are incubated for another additional 24 or 72 hours. Cell viability is estimated after addition of the CellTiter-Blue viability reagent (Promega) for 3 hours according to the protocol provided by the supplier. Cell viability the concernings normalized with respect to untreated cell rates.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 573.97
Formula

C₂₄H₃₄Cl₃F₃N₄S

CAS No. 10122-45-9
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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