Vericiguat

For research use only.

Catalog No.S9693 Synonyms: BAY1021189, Verquvo

Vericiguat Chemical Structure

CAS No. 1350653-20-1

Vericiguat (BAY1021189, Verquvo) is a potent, orally available and soluble guanylate cyclase (sGC) stimulator.

Purity & Quality Control

Choose Selective Guanylate Cyclase Inhibitors

Biological Activity

Description Vericiguat (BAY1021189, Verquvo) is a potent, orally available and soluble guanylate cyclase (sGC) stimulator.
Targets
sGC [1]
()
In vitro

The stimulation of sGC by Vericiguat is examined with a recombinant CHO cell line overexpressing rat sGC. Vericiguat stimulates the sGC reporter cell line concentration dependently with an EC50 of 1005 ± 145 nM. In the presence of the NO donor S-nitroso-N-acetyl-D,L-penicillamine (SNAP) (30 and 100 nM), the EC50 value shifts to 39.0 ± 5.1 and 10.6 ± 1.7 nM, respectively. In the presence of ODQ, pretreatment of the sGC reporter cell line with 10 μM ODQ for 3 h results in a significantly reduced efficacy of Vericiguat, with an EC50 of 256 ± 40 nM being observed.[1]

In vivo

Vericiguat can maintain heart and kidney function in a model of hypertension-induced end-organ damage with substantially reduced overall mortality.[1]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: CHO cells
  • Concentrations: 0.01 μM to 100 μM
  • Incubation Time: 6 min
  • Method:

    Cells are plated in a volume of 25 μL on white 384-well Greiner Bio-One microplates and are cultured for 1 or 2 d in medium. Medium is removed, and cells are loaded for 3 h with calcium-free Tyrode-containing coelenterazine. Serial dilutions of the test compounds (including Vericiguat) in a volume of 10 μL in calcium-free Tyrode are applied to the cells for 6 min. Thereafter, 35 μL of Tyrode-containing calcium (final concentration: 3 mM) is added to the cells and the emitted light is measured for 40 s using a CCD camera in a light-tight box. The minimal effective concentration (MEC) is determined as the concentration where a ≥3-fold increase in the basal luminescence value is observed.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: renin transgenic rats (RenTG)
  • Dosages: 3 mg/kg, 10 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 85 mg/mL (199.35 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 426.38
Formula

C19H16F2N8O2

CAS No. 1350653-20-1
Storage powder
in solvent
Synonyms BAY1021189, Verquvo

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04722562 Completed Drug: Vericiguat (BAY1021189) Heart Failure Bayer|Merck Sharp & Dohme Corp. July 16 2014 Phase 1
NCT04722484 Completed Drug: Vericiguat (BAY1021189) Heart Failure Bayer|Merck Sharp & Dohme Corp. June 12 2014 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Vericiguat | Vericiguat supplier | purchase Vericiguat | Vericiguat cost | Vericiguat manufacturer | order Vericiguat | Vericiguat distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID