ODQ

Catalog No.S9796 Synonyms: 1H-ODQ

For research use only.

ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.

ODQ Chemical Structure

CAS No. 41443-28-1

Purity & Quality Control

Choose Selective Guanylate Cyclase Inhibitors

Biological Activity

Description ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.
Targets
sGC [1]
()
In vitro

In vitro, administration of H2O2 (for 4 hrs) to rat cardiomyoblasts decreases mitochondrial respiration attributable to generation of hydroxyl radicals. Pretreatment of cells with ODQ does not abolish this cell injury. In addition, ODQ does not scavenge superoxide anions.[1]

In vivo

Pretreatment of rats with ODQ attenuates the renal dysfunction, lung injury, and hepatocellular injury caused by lipoteichoic acid/peptidoglycan or lipopolysaccharide.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: H9c2 cells
  • Concentrations: 0.1 μM to 1 mM
  • Incubation Time: 2 hrs
  • Method:

    H2O2 is a useful tool for studying the effects of reactive oxygen species on different organs. It can readily diffuse across cell membranes and can be converted into the toxic hydroxyl radical at intracellular sites via the Fenton reaction in the presence of transition metals. Cells were preincubated (2 hrs, 37°C) with ODQ (0.1 mM to 1 mM), saline, or DMSO (media containing 10% DMSO). The cells then are exposed to media or H2O2 (1 mM for 4 hrs at 37°C) after which time cell injury is assessed. All experiments are carried out in duplicate of n = 4 observations.

Animal Research:

[1]

  • Animal Models: male Wistar rats
  • Dosages: 2 mg/kg
  • Administration: IP

Solubility (25°C)

In vitro

DMSO 37 mg/mL
(197.7 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 187.15
Formula

C9H5N3O2

CAS No. 41443-28-1
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03835715 Completed Drug: Vortioxetine Major Depressive Disorder H. Lundbeck A/S February 5 2019 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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