ODQ

For research use only.

Catalog No.S9796 Synonyms: 1H-ODQ

ODQ Chemical Structure

CAS No. 41443-28-1

ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.

Purity & Quality Control

Choose Selective Guanylate Cyclase Inhibitors

Biological Activity

Description ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.
Targets
sGC [1]
()
In vitro

In vitro, administration of H2O2 (for 4 hrs) to rat cardiomyoblasts decreases mitochondrial respiration attributable to generation of hydroxyl radicals. Pretreatment of cells with ODQ does not abolish this cell injury. In addition, ODQ does not scavenge superoxide anions.[1]

In vivo

Pretreatment of rats with ODQ attenuates the renal dysfunction, lung injury, and hepatocellular injury caused by lipoteichoic acid/peptidoglycan or lipopolysaccharide.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: H9c2 cells
  • Concentrations: 0.1 μM to 1 mM
  • Incubation Time: 2 hrs
  • Method:

    H2O2 is a useful tool for studying the effects of reactive oxygen species on different organs. It can readily diffuse across cell membranes and can be converted into the toxic hydroxyl radical at intracellular sites via the Fenton reaction in the presence of transition metals. Cells were preincubated (2 hrs, 37°C) with ODQ (0.1 mM to 1 mM), saline, or DMSO (media containing 10% DMSO). The cells then are exposed to media or H2O2 (1 mM for 4 hrs at 37°C) after which time cell injury is assessed. All experiments are carried out in duplicate of n = 4 observations.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: male Wistar rats
  • Dosages: 2 mg/kg
  • Administration: IP
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 37 mg/mL (197.7 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 187.15
Formula

C9H5N3O2

CAS No. 41443-28-1
Storage powder
in solvent
Synonyms 1H-ODQ

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03835715 Completed Drug: Vortioxetine Major Depressive Disorder H. Lundbeck A/S February 5 2019 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID