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Tazemetostat hydrobromide EZH2 inhibitor

Name: Tazemetostat hydrobromide
Brand: Selleck Chemicals
SKU: E4850
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.E4850

Tazemetostat hydrobromide (EPZ-6438 hydrobromide) is a potent, selective, and orally available inhibitor of EZH2. It inhibits EZH2 with IC50 values of 11 and 16 nM in peptide assay and nucleosome assay, respectively. Tazemetostat hydrobromide also inhibits EZH1 with an IC50 of 392 nM.

Chemical Structure

Molecular Weight: 653.65

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Quality Control

Batch: Purity: 99.50%

99.50

Related Targets	HDAC JAK BET Histone Methyltransferase PKC PARP HIF PRMT AMPK Histone Acetyltransferase
Other EZH2 Inhibitors	PF-06821497 GSK126 Tulmimetostat (CPI-0209) Valemetostat (DS-3201) GSK343 Lirametostat (CPI-1205) CPI-169 MS1943 SHR2554 EZH2/HSP90-IN-29

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (152.98 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (7.65mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.600mg/ml (0.92mM)	Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	653.65	Formula	C₃₄H₄₄N₄O₄.BrH	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1467052-75-0	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Read more about storage stability stock solution CAS number

Mechanism of Action

Targets/IC₅₀/Ki	EZH2 11 nM EZH2 16 nM EZH1 392 nM
References	[1] https://pubmed.ncbi.nlm.nih.gov/23620515/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-04-02)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05994235	ACTIVE_NOT_RECRUITING	Follicular Lymphoma	Weill Medical College of Cornell University	2023-11-01	PHASE2
NCT06824701	ACTIVE_NOT_RECRUITING	Relapsed or Refractory B-cell Non-Hodgkin Lymphoma	University of Utah	2025-09-12	PHASE1
NCT06068881	WITHDRAWN	Follicular Lymphoma; Refractory Follicular Lymphoma	Epizyme, Inc.	2025-09-02	PHASE2
NCT06575686	SUSPENDED	Recurrent Grade 1 Follicular Lymphoma; Recurrent Grade 2 Follicular Lymphoma; Recurrent Grade 3a Follicular Lymphoma; Refractory Grade 1 Follicular Lymphoma; Refractory Grade 2 Follicular Lymphoma; Refractory Grade 3a Follicular Lymphoma	City of Hope Medical Center	2025-03-11	PHASE2
NCT05627232	RECRUITING	Recurrent Acute Myeloid Leukemia; Refractory Acute Myeloid Leukemia	Thomas Jefferson University	2023-08-28	PHASE1
NCT07407283	RECRUITING	Prostate Cancer	Jiangsu HengRui Medicine Co., Ltd.	2026-04-08	PHASE1; PHASE2

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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