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Suc-Leu-Leu-Val-Tyr-AMC

Name: Suc-Leu-Leu-Val-Tyr-AMC
Brand: Selleck Chemicals
SKU: E7007
Rating: 5 (1 reviews)

Synonyms: Succinyl-leucyl-leucyl-valyl-tyrosyl-methylcoumarinamide

Catalog No.E7007 Purity:95.33%

Suc-Leu-Leu-Val-Tyr-AMC (Succinyl-leucyl-leucyl-valyl-tyrosyl-methylcoumarinamide) is a fluorescent substrate for the 20S proteasome, other chymotrypsin-like proteases, and calpains. Upon enzymatic cleavage, it releases the fluorescent AMC (7-amino-4-methylcoumarin) with an excitation maximum at 360 nm and an emission maximum at 460 nm.

Suc-Leu-Leu-Val-Tyr-AMC Chemical Structure

CAS No. 94367-21-2

Purity & Quality Control

Batch: E700701 DMSO]100 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 95.33%

95.33

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Biological Activity

Description	Suc-Leu-Leu-Val-Tyr-AMC (Succinyl-leucyl-leucyl-valyl-tyrosyl-methylcoumarinamide) is a fluorescent substrate for the 20S proteasome, other chymotrypsin-like proteases, and calpains. Upon enzymatic cleavage, it releases the fluorescent AMC (7-amino-4-methylcoumarin) with an excitation maximum at 360 nm and an emission maximum at 460 nm.

References

https://pubmed.ncbi.nlm.nih.gov/9974389/
https://pubmed.ncbi.nlm.nih.gov/6092335/
https://pubmed.ncbi.nlm.nih.gov/25151970/

Chemical Information & Solubility

Molecular Weight	763.88	Formula	C₄₀H₅₃N₅O₁₀
CAS No.	94367-21-2	SDF	Download Suc-Leu-Leu-Val-Tyr-AMC SDF
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (130.91 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.25mg/ml (1.64mM)	Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.7mg/ml (0.92mM)	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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