SGC-CBP30

Catalog No.S7256

For research use only.

SGC-CBP30 is a potent CREBBP/EP300 inhibitor with IC50 of 21 nM and 38 nM in cell-free assays, respectively. Exhibits 40-fold and 250-fold selectivity for CBP over the first BRD of BRD4 (BRD4(1)) and BRD4(2) respectively.

SGC-CBP30 Chemical Structure

CAS No. 1613695-14-9

Selleck's SGC-CBP30 has been cited by 24 publications

Purity & Quality Control

Choose Selective Epigenetic Reader Domain Inhibitors

Biological Activity

Description SGC-CBP30 is a potent CREBBP/EP300 inhibitor with IC50 of 21 nM and 38 nM in cell-free assays, respectively. Exhibits 40-fold and 250-fold selectivity for CBP over the first BRD of BRD4 (BRD4(1)) and BRD4(2) respectively.
Features CREBBP/EP300-selective chemical probe.
Targets
CREBBP [1]
(Cell-free assay)
EP300 [1]
(Cell-free assay)
21 nM 38 nM
In vitro

As a CREBBP/EP300-selective chemical probe, SGC-CBP30 shows moderate cytotoxicity in U2OS cells and HeLa cells. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RKO NUe1S4J{TnWwY4Tpc44h[XO|YYm= NUHTW242OjRiaILz M2X4[mlvcGmkaYTpc44hd2ZiZH;4c5J2[mmlaX6td5RqdXWuYYTl[EBxPTNiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gbJVu[W5iUlvPJINmdGy|IIDy[Ylv[3WkYYTl[EBnd3JiMkSgbJJ{KG[xbHzve4VlKGK7IHHk[Il1cW:wIH;mJIRwgG:{dXLpZ4lvKG[xcjCxOkBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeTygTWM2OCB;IEGuOUDPxE1w MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV5MkKxO{c,OjZ3N{KyNVc9N2F-
AMO1 MVHGeY5kfGmxbjDhd5NigQ>? MVy2JIhzew>? NFzlN49KdmirYnn0bY9vKG:oIFPCVE9GWDNyMDDpckBpfW2jbjDBUW8yKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDNXWMh\XiycnXzd4lwdiCjZoTldkA3KGi{czDifUByfWGwdHnn[Y5mKHCuZYigZZN{[XluIFXDOVAhRSB{Lkeg{txONg>? NUjudVBSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexPVA3ODVpPkK3NVkxPjB3PD;hQi=>
HEK293 NVXaRVlKTnWwY4Tpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKEijbH:teIFo\2WmIHjpd5RwdmViSEOuN{BjcW6maX7nJJRwKE6jbn;MeYMhdHWlaX\ldoF{\SClb37qeYdifGWmIFPCVEApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZYZ1\XJib4\ldo5q\2i2IHnuZ5Vj[XSrb36gZpkhSlKHVDDhd5NigSxiSVO1NEA:KDJwODFOwG0v NYfmZ5hURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PFI2ODdpPkK3OlgzPTB5PD;hQi=>
RKO MlPwSpVv[3Srb36gZZN{[Xl? MYGyOEBpenN? M3viTWlvcGmkaYTpc44hd2ZicEWzMYlv\HWlZXSgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hcHWvYX6gVmtQKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDwOVMucW6mdXPl[EBxOjFiYXP0bZZifGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNU42PCEQvF2u M1TLb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFM3OjJ|N{OvK|5EcEWPQly8M4E,
Assay
Methods Test Index PMID
Western blot IKZF1 / IKZF3 / p-STAT3 / STAT3 / IRF4 / Myc 30741931
Growth inhibition assay Cell viability 30741931

Protocol (from reference)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 509.04
Formula

C28H33ClN4O3

CAS No. 1613695-14-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(=NO1)C)C2=CC3=C(C=C2)N(C(=N3)CCC4=CC(=C(C=C4)OC)Cl)CC(C)N5CCOCC5

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
The recommend formulation of SGC-CBP30 (Cat S7256) for in vivo studies on your website is “2% DMSO+30% PEG 300+5% Tween 80+ddH2O”. Is this a suspension or clear solution?

Answer:
S7256 SGC-CBP30 in 2% DMSO+30% PEG 300+5% Tween 80+ddH2O at 5mg/ml is a clear solution.

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