Semaglutide Sodium

Synonyms: Rybelsus, Ozempic, NN9535, OG217SC, NNC 0113-0217

Semaglutide Sodium (Rybelsus, Ozempic, NN9535, OG217SC, NNC 0113-0217), a long-acting glucagon-like peptide 1 (GLP-1) analogue, is a GLP-1 receptor agonist with the potential for the treatment of type 2 diabetes mellitus (T2DM).

Semaglutide Sodium Chemical Structure

Semaglutide Sodium Chemical Structure

CAS No. 910463-68-2(free base)

Purity & Quality Control

Semaglutide Sodium Related Products

Biological Activity

Description Semaglutide Sodium (Rybelsus, Ozempic, NN9535, OG217SC, NNC 0113-0217), a long-acting glucagon-like peptide 1 (GLP-1) analogue, is a GLP-1 receptor agonist with the potential for the treatment of type 2 diabetes mellitus (T2DM).
Targets
GLP-1 receptor [1]
In vitro
In vitro

Semaglutide is selected as the optimal once weekly candidate. Semaglutide has two amino acid substitutions compared to human GLP-1 (Aib8, Arg34) and is derivatized at lysine 26. The GLP-1R affinity of semaglutide (0.38 ± 0.06 nM) is three-fold decreased compared to liraglutide, whereas the albumin affinity is increased.[2]

Cell Research Cell lines BHK cells
Concentrations 0.01 pM - 0.1 μM
Incubation Time 3 h
Method

Frozen aliquots of BHK cells that express both the hGLP-1R and CRE firefly luciferase (clone FCW467-12A/KZ10-1) are thawed, washed twice in PBS, and suspended in assay buffer. Cells are plated out into 96-well plates at 5000 cells/well in a volume of 50 μL. Compounds to be tested are diluted in assay buffer and a 50 μL aliquot transferred to the plate containing the cells to reach final assay concentrations of 1 × 10−14 − 1 × 10−7 M. The plate is incubated for 3 h at 5% CO2 at 37 °C. The plate was allowed to stand at room temperature for 15 min prior to adding 100 μL of steadylite plus reagent. The plate is covered to protect it from light and shaken at room temperature for 30 min. The plate is read in a TopCount NXT instrument.

In Vivo
In vivo

The plasma half-life is 46.1 h in mini-pigs following i.v. administration, and semaglutide has an MRT of 63.6 h after s.c. dosing to mini-pigs.[2]

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05714683 Not yet recruiting
Diabetes Mellitus Type 2
Novo Nordisk A/S
December 31 2024 --
NCT05689372 Not yet recruiting
Diabetes Mellitus Type 2
Novo Nordisk A/S
June 30 2024 --
NCT06015893 Recruiting
Addiction|Alcohol Use Disorder
National Institute on Drug Abuse (NIDA)|National Institutes of Health Clinical Center (CC)
May 15 2024 Phase 2

Chemical Information & Solubility

Molecular Weight 4113.58(free base) Formula

C187H291N45O59

CAS No. 910463-68-2(free base) SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

4-Methylpyridine : 4 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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