MK-8776 (SCH 900776)

For research use only.

Catalog No.S2735

41 publications

MK-8776 (SCH 900776) Chemical Structure

CAS No. 891494-63-6

MK-8776 (SCH 900776) is a selective Chk1 inhibitor with IC50 of 3 nM in a cell-free assay. It shows 500-fold selectivity against Chk2. Phase 2.

Size Price Stock Quantity  
10mM (1mL in DMSO) RMB 1921.21 In stock
RMB 1733.35 In stock
RMB 3015.42 In stock
RMB 7966.05 In stock
RMB 20229.30 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's MK-8776 (SCH 900776) has been cited by 41 publications

Purity & Quality Control

Choose Selective Chk Inhibitors

Biological Activity

Description MK-8776 (SCH 900776) is a selective Chk1 inhibitor with IC50 of 3 nM in a cell-free assay. It shows 500-fold selectivity against Chk2. Phase 2.
Targets
Chk1 [1]
(Cell-free assay)
CDK2 [1]
(Cell-free assay)
3 nM 0.16 μM
In vitro

SCH 900776 is a less potent inhibitor of Chk2 and CDK2 with IC50 of 1.5 μM and 0.16 μM, respectively. SCH 900776 shows no significant inhibition of cytochrome P450 human liver microsomal isoforms 1A2, 2C9, 2C19, 2D6, and 3A4. SCH 900776 induces a dose-dependent loss of DNA replication capability 24 hours after hydroxyurea exposure. SCH 900776 enhances the γ-H2AX response of hydroxyurea, 5-fluoruracil, and cytarabine. In combination with an antimetabolite, SCH 900776 induces accumulation of γ-H2AX within 2 hours, indicative of replication fork collapse and double stranded DNA breaks. Additionally, SCH 900776 suppresses accumulation of the Chk1 pS296 autophosphorylation in a dose-dependent manner. Exposure of proliferating WS1 cells to SCH 900776 is associated with rapid, dose-dependent accumulation of Chk1 pS345, indicating that cycling populations of normal cells induce Chk1 pS345 following exposure to SCH 900776 as part of a futile cycle, perhaps driven by AT-family kinases and DNA-PK.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U251 MnqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7r[GtqOjByL{KwNFAhdk1? NHH5dHQzPCCq MX\k[YNz\WG|ZYOgeIhmKEmFNUCgc4YhT2WvY3n0ZYJqdmV? M1XLTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W5OVI3Lz5{NEO1PVUzPjxxYU6=
HCT115 M2HZdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY[yNFAwOjByMDDuUS=> Mof6NlQhcA>? NX;mdWY4\GWlcnXhd4V{KHSqZTDJR|UxKG:oIFflcYNqfGGkaX7l M2DOUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W5OVI3Lz5{NEO1PVUzPjxxYU6=
SW620 MlvZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3YNlAxNzJyMECgcm0> MkLMNlQhcA>? MYrk[YNz\WG|ZYOgeIhmKEmFNUCgc4YhT2WvY3n0ZYJqdmV? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OUWyOkc,OjR|NUm1NlY9N2F-
IGROV-1 NHHtRYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\Sc|IxOC9{MECwJI5O MUKyOEBp M{izR4Rm[3KnYYPld{B1cGViSVO1NEBw\iCJZX3jbZRi[mmwZR?= NG\K[3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PVUzPid-MkSzOVk2OjZ:L3G+
HCT116 NWXsWW81T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTTPI9lOjByL{KwNFAhdk1? NVPETmY1OjRiaB?= Mlzp[IVkemWjc3XzJJRp\SCLQ{WwJI9nKEenbXPpeIFjcW6n NWnKRmtCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVk2OjZpPkK0N|U6PTJ4PD;hQi=>
MCF10A NUDmSZpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\4RVhbOjByL{KwNFAhdk1? NGPsVHEzPCCq Mkj3[IVkemWjc3XzJJRp\SCLQ{WwJI9nKEenbXPpeIFjcW6n NVrnUlZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVk2OjZpPkK0N|U6PTJ4PD;hQi=>
MiaPaCa-2 NXPKWI9KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWWyNFAwOjByMDDuUS=> NFfkcWYzPCCq Mmj1[IVkemWjc3XzJJRp\SCLQ{WwJI9nKEenbXPpeIFjcW6n NX\JNVVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVk2OjZpPkK0N|U6PTJ4PD;hQi=>
MDA-MB-231 NEHMZ|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLCNlAxNzJyMECgcm0> MXuyOEBp NGPPUZBl\WO{ZXHz[ZMhfGinIFnDOVAhd2ZiR3XtZ4l1[WKrbnW= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OUWyOkc,OjR|NUm1NlY9N2F-
HCC2998 NXP1R3BJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvYVHg5OjByL{KwNFAhdk1? NFv1c3MzPCCq M2K4WIRm[3KnYYPld{B1cGViSVO1NEBw\iCJZX3jbZRi[mmwZR?= NWG2WVN{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVk2OjZpPkK0N|U6PTJ4PD;hQi=>
U87 NWLBVIZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWmyNFAwOjByMDDuUS=> MVOyOEBp MkXE[IVkemWjc3XzJJRp\SCLQ{WwJI9nKEenbXPpeIFjcW6n NXnn[281RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVk2OjZpPkK0N|U6PTJ4PD;hQi=>
MDA-MB-435 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIT2bWEzODBxMkCwNEBvVQ>? Ml7PNlQhcA>? MlLv[IVkemWjc3XzJJRp\SCLQ{WwJI9nKEenbXPpeIFjcW6n NV\JeY5xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVk2OjZpPkK0N|U6PTJ4PD;hQi=>
SNB19 NWjEUHN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nGbFIxOC9{MECwJI5O NX7LfIhYOjRiaB?= NV\WN49T\GWlcnXhd4V{KHSqZTDJR|UxKG:oIFflcYNqfGGkaX7l M2ribFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W5OVI3Lz5{NEO1PVUzPjxxYU6=
U20S M4HjO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfSXmMzODBxMkCwNEBvVQ>? NIrwcFIzPCCq MVjk[YNz\WG|ZYOgeIhmKEmFNUCgc4YhT2WvY3n0ZYJqdmV? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OUWyOkc,OjR|NUm1NlY9N2F-
A498 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHGyTXkzODBxMkCwNEBvVQ>? NWLXRVJyOjRiaB?= M4nCT4Rm[3KnYYPld{B1cGViSVO1NEBw\iCJZX3jbZRi[mmwZR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN3OUWyOkc,OjR|NUm1NlY9N2F-
TK10 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrsNlAxNzJyMECgcm0> NEPjSmQzPCCq M{XUS4Rm[3KnYYPld{B1cGViSVO1NEBw\iCJZX3jbZRi[mmwZR?= M1TDVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{W5OVI3Lz5{NEO1PVUzPjxxYU6=
AsPC-1 NV\aeo1RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1e4VFIxOC9{MECwJI5O NWrZcYVYOjRiaB?= NF3ZSlNl\WO{ZXHz[ZMhfGinIFnDOVAhd2ZiR3XtZ4l1[WKrbnW= NGP3RVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1PVUzPid-MkSzOVk2OjZ:L3G+
H23 NV;yVFZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHWXo5bPTByIH7N M{POTlI1KGh? MofGSG1UVw>? MY\lcohidmOnczD0bIUh[2inbX;z[Y5{cXSrenH0bY9vKHSxIGDNXC=> NUnGUlI4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVM2PDlpPkK0NVE{PTR7PD;hQi=>
H1437 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ru[lUxOCCwTR?= MmToNlQhcA>? MlTCSG1UVw>? MWnlcohidmOnczD0bIUh[2inbX;z[Y5{cXSrenH0bY9vKHSxIGDNXC=> NHGwToY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGxN|U1QSd-MkSxNVM2PDl:L3G+
H1993 MnyyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPmVI4zPTByIH7N NIDhe2wzPCCq NX3WfG5mTE2VTx?= MVHlcohidmOnczD0bIUh[2inbX;z[Y5{cXSrenH0bY9vKHSxIGDNXC=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzM{W0PUc,OjRzMUO1OFk9N2F-
H1299 NHq0NZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjMOoJLPTByIH7N NYPRSYlpOjRiaB?= M1HIUGROW09? NGDIeo5mdmijbnPld{B1cGViY3jlcY9{\W6|aYTpfoF1cW:wIITvJHBOYA>? MkTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMUO1OFkoRjJ2MUGzOVQ6RC:jPh?=
AsPC-1 NVPaO4JuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\KNVAuOTByMDDuUS=> M1XzS|I1NTR6aB?= NYDZb4dw\W6qYX7j[ZMhfGinIHPo[Y1we2Wwc3n0bZpifGmxbjD0c{Bo\W2laYThZolv\Q>? M4XlblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC0OFIzLz5{M{iwOFQzOjxxYU6=
MiaPaCa-2 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXP0TZB7OTBvMUCwNEBvVQ>? NIPrOYUzPC12OHi= NI\kZlBmdmijbnPld{B1cGViY3jlcY9{\W6|aYTpfoF1cW:wIITvJIdmdWOrdHHibY5m NIG1VpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwOFQzOid-MkO4NFQ1OjJ:L3G+
BxPC-3 NX;Q[ZRYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV3IdYpPOTBvMUCwNEBvVQ>? NUfNbmNOOjRvNEjo M{fTdoVvcGGwY3XzJJRp\SClaHXtc5NmdnOrdHn6ZZRqd25idH:g[4Vu[2m2YXLpcoU> M{TKW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC0OFIzLz5{M{iwOFQzOjxxYU6=
SKOV3 MoHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoP1NE4{KML3TR?= M2rXdlgh\A>? MU\z[Y5{cXSrenXzJJRp\SClZXzsJIxqdmW|IITvJIdmdWOrdHHibY5myqB? NGHt[4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W0PFI3QSd-MkO1OFgzPjl:L3G+
OVCAR-8 NFjUS3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGG3UXcxNjNiwsXN Mof1PEBl NXrGdJlke2Wwc3n0bZpmeyC2aHWgZ4VtdCCuaX7ld{B1dyCpZX3jbZRi[mmwZdMg M1:1N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS4NlY6Lz5{M{W0PFI3QTxxYU6=
MV-4-11 MWjBdI9xfG:|aYOgRZN{[Xl? NWSzeoFGOTByLUewNEBvVQ>? NWDvU5l5PDhiaB?= MVrpcoR2[2W|IHHwc5B1d3OrczDkc5NmKGSncHXu[IVvfGy7 NWPKXFNvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N|Y4OjFpPkKzOVM3PzJzPD;hQi=>
U937 Mo\JRZBweHSxc3nzJGF{e2G7 MlXINVAxNTdyMDDuUS=> MWi0PEBp Mkj6bY5lfWOnczDhdI9xfG:|aYOg[I9{\SCmZYDlcoRmdnSueR?= NF\Ic2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzOlczOSd-MkO1N|Y4OjF:L3G+
MOLM-13  MlvXRZBweHSxc3nzJGF{e2G7 M4LPT|ExOC15MECgcm0> NIHsfJc1QCCq M1izdolv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= M3jvNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUO2O|IyLz5{M{WzOlczOTxxYU6=
A2058  NH;0R2RE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NIXsfJA{Py53LUOwNEBvVQ>? M3HkRlczKGh? M{nLO2ROW09? MUTy[YR2[2W|IITo[UBOUy1zN{e1JGVEPTEEoHL5JFUu\m:uZDD0c{BidiCjdnXyZYdmKG:oIES1JI5O MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF2OE[4OEc,OjNzNEi2PFQ9N2F-
H2009 Mke2R4VtdCCYaXHibYxqfHliQYPzZZk> NXy4e|dpPTByIH7N M2LxN|czKGh? MnHZSG1UVw>? NWfr[VltemW|dXz0d{BqdiCJMT;TMZBp[XOnIHHjZ5VufWyjdHnvckBkd22kaX7l[EB4cXSqIF3LMVE4PzV? Mn6zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNEi2PFQoRjJ|MUS4Olg1RC:jPh?=
Su.86.86 MlH1R4VtdCCYaXHibYxqfHliQYPzZZk> NFezW5c2ODBibl2= MlKxO|IhcA>? NH;PeWhFVVOR MoG5doV{fWy2czDpckBIOS:VLYDoZZNmKGGlY4XteYxifGmxbjDjc41jcW6nZDD3bZRpKE2NLUG3O|U> NXHUcZQyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOFg3QDRpPkKzNVQ5Pjh2PD;hQi=>
HRE MV7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NFK0b4M2ODBibl2= MYm3NkBp MUfEUXNQ NGTkS|Jz\XO3bITzJIlvKEdzL2OtdIhie2ViYXPjeY12dGG2aX;uJINwdWKrbnXkJJdqfGhiTVutNVc4PQ>? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF2OE[4OEc,OjNzNEi2PFQ9N2F-
HMEC MmXkR4VtdCCYaXHibYxqfHliQYPzZZk> NGr6RpU2ODBibl2= MXq3NkBp M1HhN2ROW09? NYXkTVF2emW|dXz0d{BqdiCJMT;TMZBp[XOnIHHjZ5VufWyjdHnvckBkd22kaX7l[EB4cXSqIF3LMVE4PzV? NH[yOWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G0PFY5PCd-MkOxOFg3QDR:L3G+
U2OS  MW\GeY5kfGmxbjDBd5NigQ>? MlnyNkDDvU1? M3rmbFAuOjRiaB?= NXzle|B2cW6mdXPld{BxcG:|cHjvdplt[XSrb36gc4YhS2itMTDheEB{\XKrbnWgN|Q2KGG2IHLveIgh[2:wY3XueJJifGmxboOgZZMh\WG{bImgZZMhOiCqIHHmeIVzKGGmbXnubZN1emG2aX;u NWfUc5ZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5N|cyPDdpPkKyPVM4OTR5PD;hQi=>
U2OS  MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrOUGIxNTFyINM1US=> NVjocWw2OjRxNEigbC=> NITDSpRqdmirYnn0d{Bk\WyuIHfyc5d1cCCmb4PlJIRmeGWwZHXueIx6 M3flblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUO3NVQ4Lz5{MkmzO|E1PzxxYU6=
U937 NIDCNXdHfW6ldHnvckBCe3OjeR?= NYfGRoo5OTByLUWwNEBvVQ>? M2i3PVQhcMLi NVP0W25n\GWlcnXhd4V{KHSqZTDjfZRiemGkaX7lMYlv\HWlZXSgR4hsOSCjdYTvdIhwe3Cqb4L5cIF1cW:wIHH0JHNmejJ7NtMgZY5lKHC{ZY\lcpR{KEOmY{K1RUBld3ewcnXneYxifGmxbh?= NIG2UWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki2PVg3QSd-MkK4Olk5Pjl:L3G+
U937 NYTBTWZNTnWwY4Tpc44hSXO|YYm= NFXQfYUyODBibl2= NXjQcVBWPCCqwrC= NH[zSZRz\X[ncoPld{B1cGViY4n0ZZJi[mmwZT3pcoR2[2WmIHnubIljcXSrb36gc4bDqDOKLYTofY1q\GmwZTDpcoNwenCxcnH0bY9vKGmwdH:gSG5C NHT0ZVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki2PVg3QSd-MkK4Olk5Pjl:L3G+
U937 MYXGeY5kfGmxbjDBd5NigQ>? NIGyRWUyODBvNUCwJI5O NHzCSWo1KGkEoB?= M3;FWolv\HWlZYOgbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBJOkG[ MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh4OUi2PUc,OjJ6Nkm4Olk9N2F-
HL-60 MnjvRZBweHSxc3nzJGF{e2G7 NGjCUYc{OC9zMECvN|AxKG6P M2TzO|I1KGh? M4jsRmROW09? Mlv5[Y5p[W6lZYOgZ5l1[XKjYnnu[U1qdmS3Y3XkJIFxd3C2b4Ppdy=> MkT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6Nkm4OlkoRjJ{OE[5PFY6RC:jPh?=
ML-1 MkLQRZBweHSxc3nzJGF{e2G7 NIjPV3czPS93MD:xNFAhdk1? NVPKW482OjRiaB?= M1Pid2ROW09? NXXIZXQz\W6qYX7j[ZMh[3m2YYLhZolv\S2rbnT1Z4VlKGGyb4D0c5Nqew>? Mn\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6Nkm4OlkoRjJ{OE[5PFY6RC:jPh?=
HCT116 MYXGeY5kfGmxbjDBd5NigQ>? MUCxJOK2VQ>? M{Pwd|I1KGh? NYfuendG[WK{b3fheIV{KG:oIHPlcIwh[3mlbHWgZZJz\XO2wrC= NEL3eW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNFU3OCd-MkK1NVA2PjB:L3G+
U2OS NETtNmdHfW6ldHnvckBCe3OjeR?= NV;Db5k{OSEEtV2= NWPtW2xuOjRiaB?= MVHhZpJw\2G2ZYOgc4Yh[2WubDDjfYNt\SCjcoLld5TDqA>? NF24Tpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWxNFU3OCd-MkK1NVA2PjB:L3G+
Sf9 NE[3[VlHfW6ldHnvckBie3OjeR?= NWPqeFVXUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBETEt{L1P5Z4xqdiCDIHX4dJJme3OnZDDpckBqdnOnY4SgV4Y6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gX|M{WF1vQWTQJIlv[2:{cH;yZZRqd25iaX70c{BjcW:2aX75cIF1\WRiaHnzeI9v\SCKMTDh[pRmeiBzIHjyJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDJR|UxKD1iMD6xOkDPxE1w M{Tj[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEm0OlA4Lz5{MUC5OFYxPzxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-chk1(ser345) / CDC25A ; 

PubMed: 27690219     


Western blots for H1299 and A549 cells treated with MK-8776 for various times and assessed for expression of p-chk1 (ser345) and CDC25A.

Cyclin E / pY15-CDK / γH2AX ; 

PubMed: 26595527     


The indicated cell lines were incubated with 2 μM of each drug for 6 h, then lysed and analyzed by western blotting. The top row reflects cells sensitive to MK-8776. The second row reflects cells that are also sensitive to MK-8776, but which fail to degrade cyclin E. The third row reflects MK-8776-resistant cell lines.

27690219 26595527
In vivo Administered 30 minutes after gemcitabine, 4 mg/kg SCH 900776 is sufficient to induce the γ-H2AX biomarker while 8 mg/kg leads to enhanced tumor pharmacodynamic and regression responses relative to gemcitabine or SCH 900776 alone. Dose escalation of SCH 900776 (16 mg/kg and 32 mg/kg) induces incremental improvements in tumor response. Importantly, doses of SCH 900776 associate with robust biomarker activation and improved tumor response are not associated with enhanced toxicity of gemcitabine on hematological parameters in BALB/c mice. [1]

Protocol

Animal Research:[1]
- Collapse
  • Animal Models: Female nude mice injected subcutaneously with A2780 or MiaPaCa2 cells
  • Dosages: ~50 mg/kg
  • Administration: Administered intraperitoneally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 3 mg/mL (7.97 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% propylene glycol
For best results, use promptly after mixing.
5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 376.25
Formula

C15H18BrN7

CAS No. 891494-63-6
Storage powder
in solvent
Synonyms N/A
Smiles CN1C=C(C=N1)C2=C3N=C(C(=C(N3N=C2)N)Br)C4CCCNC4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00779584 Completed Drug: MK-8776|Drug: Gemcitabine Hodgkin Disease|Lymphoma Non-Hodgkin|Neoplasms Merck Sharp & Dohme Corp. October 17 2008 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I would like to know whether your product S2735 is the optically pure R enantiomer or whether it is a racemic mix.

  • Answer:

    Our S2735 MK-8776 (SCH 900776) is R enantiomer.

Chk Signaling Pathway Map

Chk Inhibitors with Unique Features

Related Chk Products

Tags: buy MK-8776|MK-8776 ic50|MK-8776 price|MK-8776 cost|MK-8776 solubility dmso|MK-8776 purchase|MK-8776 manufacturer|MK-8776 research buy|MK-8776 order|MK-8776 mouse|MK-8776 chemical structure|MK-8776 mw|MK-8776 molecular weight|MK-8776 datasheet|MK-8776 supplier|MK-8776 in vitro|MK-8776 cell line|MK-8776 concentration|MK-8776 nmr|MK-8776 in vivo|MK-8776 clinical trial|MK-8776 inhibitor|MK-8776 Cell Cycle inhibitor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID