AZD7762

For research use only.

Catalog No.S1532

68 publications

AZD7762 Chemical Structure

Molecular Weight(MW): 362.42

AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.

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Selleck's AZD7762 has been cited by 68 publications

Purity & Quality Control

Choose Selective Chk Inhibitors

Biological Activity

Description AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.
Targets
Chk1 [1]
(Cell-free assay)
Chk2 [1]
(Cell-free assay)
5 nM <10 nM
In vitro

AZD7762, a more selective Chk1 inhibitor, inhibits Chk1 phosphorylation of a cdc25C peptide by reversibly binding to the ATP-binding site of Chk1, with IC50 of 5 nM and Ki of 3.6 nM. AZD7762 induces cell arrest with EC50 of 0.620 μM, and significantly abrogates the camptothecin induced G2 arrest with an EC50 of 10 nM, by blocking the chk1 dependent degradation of Cdc25A and activation of Cyclin A. AZD7762 (300 nM) enhances the antitumor efficacy of gemcitabine against SW620 and topotecan against MDA-MB-231 by reducing the GI50 values from 24.1 nM and 2.25 μM to 1.08 nM and 0.15 μM, respectively. [1] AZD7762 shows cytotoxicity against a variety of neuroblastoma cell lines bearing p53 wild type, p53 mutation, Mdm2 amplification or p14 deletion with IC50 values ranging from 82.6-505.9 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KASUMI-1 NIG0UpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvuWWpPUUN3ME2wMlAxODJ|NDFOwG0> NXHJV5BDW0GQR1XS
NKM-1 Mn3wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml6wTWM2OD1yLkCwNFQyPSEQvF2= MXHTRW5ITVJ?
MV-4-11 Mn\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{ex[WlEPTB;MD6wNFA1OjVizszN NWrUVVhIW0GQR1XS
NCI-H1703 M37wTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1izdWlEPTB;MD6wNFY1OyEQvF2= NVjtcnNXW0GQR1XS
A204 Mn7DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojETWM2OD1yLkCwPFU3KM7:TR?= M3eyTHNCVkeHUh?=
CGTH-W-1 NGXtO4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDW[WVWUUN3ME2wMlAyPDJizszN M{jVS3NCVkeHUh?=
MLMA MlHOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTBwMEK4OFkh|ryP MkfaV2FPT0WU
GOTO MnTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLkTWM2OD1yLkCzNVIh|ryP M{fJe3NCVkeHUh?=
KE-37 Mne1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoO5TWM2OD1yLkCzNVQzKM7:TR?= M1nvenNCVkeHUh?=
ES4 NITPZ2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTEc3VtUUN3ME2wMlA{OjVzIN88US=> NX7xNIlkW0GQR1XS
ATN-1 NIXkNW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjtVm55UUN3ME2wMlA{PDF5IN88US=> MoLnV2FPT0WU
MHH-PREB-1 NGXKd4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTBwMEO0OFIh|ryP MVfTRW5ITVJ?
SW756 Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3u1eGlEPTB;MD6wN|U4QSEQvF2= MVTTRW5ITVJ?
CTV-1 M3OwXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\JSJpKSzVyPUCuNFM5QTZizszN NVPB[4N2W0GQR1XS
HCC1806 MkHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLGTWM2OD1yLkC0NFIzKM7:TR?= M37ySHNCVkeHUh?=
NEC8 Mlu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEmwR4hKSzVyPUCuNFQzODNizszN M1j5SnNCVkeHUh?=
P12-ICHIKAWA NITTdYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrBPJBKSzVyPUCuNFQ{OTZizszN MUfTRW5ITVJ?
DEL NWTEdmFvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrOTWM2OD1yLkC0OVkzKM7:TR?= NXLOfWpsW0GQR1XS
KM12 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3e1TGlEPTB;MD6wOVE2QCEQvF2= NEXOOJdUSU6JRWK=
LB1047-RCC NXXJdm9UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVT0b5lmUUN3ME2wMlA3ODJ3IN88US=> NGrJU3BUSU6JRWK=
LB2241-RCC MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPveHdKSzVyPUCuNFYyODJizszN Ml\aV2FPT0WU
NOMO-1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTBwME[xNVUh|ryP MUjTRW5ITVJ?
NB14 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFG2SmtKSzVyPUCuNFYyOzZizszN MVPTRW5ITVJ?
ONS-76 NEKxWYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nGd2lEPTB;MD6wOlE6PiEQvF2= M3;5[nNCVkeHUh?=
HD-MY-Z M3\vOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnoN|NKUUN3ME2wMlA3OjV3IN88US=> NIjucGVUSU6JRWK=
G-402 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;LVYRKSzVyPUCuNFY1PiEQvF2= MYLTRW5ITVJ?
ES1 NXX6co9bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjnV2dKSzVyPUCuNFcyPDZizszN NUTJdYQ5W0GQR1XS
L-363 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Oze2lEPTB;MD6wO|M6KM7:TR?= MXrTRW5ITVJ?
A4-Fuk MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XwNWlEPTB;MD6wO|Q5PiEQvF2= MmDwV2FPT0WU
OC-314 Ml;1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonrTWM2OD1yLkC3PFA4KM7:TR?= NXjYXGlPW0GQR1XS
SK-LU-1 NXu1RYx2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17YbmlEPTB;MD6wPFQzOSEQvF2= MlHyV2FPT0WU
MDA-MB-157 NEDZcGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTBwMEi2OVkh|ryP NV21XYFqW0GQR1XS
HuP-T3 MnixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3WR2tLUUN3ME2wMlA5PzFizszN MVnTRW5ITVJ?
A3-KAW Ml;FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTBwMEi4NFYh|ryP NUfh[Vd2W0GQR1XS
MOLT-16 MmftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;UWIhKSzVyPUCuNFg5ODdizszN MUjTRW5ITVJ?
COLO-679 NXTnbG5oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTBwMEmwPVEh|ryP MXvTRW5ITVJ?
EM-2 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3YPZJEUUN3ME2wMlA6QTB|IN88US=> MmK1V2FPT0WU
ES8 NEnWU|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTBwMEm5NVgh|ryP NVfqdmc3W0GQR1XS
SK-MEL-2 M4Pzdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2qwTGlEPTB;MD6xNFA{OyEQvF2= NYr3NGN7W0GQR1XS
ES3 M4C2W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnk[5JQUUN3ME2wMlExOiEQvF2= M{HOO3NCVkeHUh?=
HSC-4 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnwTndKSzVyPUCuNVA2PTRizszN NUfT[XB6W0GQR1XS
697 M4XGT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXqNJRKSzVyPUCuNVExPjNizszN MonlV2FPT0WU
TGBC11TKB NUXZfoQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmqwTWM2OD1yLkGxNFk{KM7:TR?= M2C1cnNCVkeHUh?=
SK-PN-DW MlPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWezOGNSUUN3ME2wMlEyQDZizszN NEnHRpNUSU6JRWK=
H9 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHZS45LUUN3ME2wMlEzOTZ3IN88US=> MX;TRW5ITVJ?
NCI-H2122 MmfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHFOnNKSzVyPUCuNVIzPSEQvF2= MlTIV2FPT0WU
ML-2 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofTTWM2OD1yLkGyOFg1KM7:TR?= MnHLV2FPT0WU
MC-IXC MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnyOGpKSzVyPUCuNVI5PDNizszN NXTlR5pDW0GQR1XS
KYSE-140 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEP2bohKSzVyPUCuNVMxPDFizszN MmjCV2FPT0WU
DOHH-2 NUL1OpRwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3YblJKSzVyPUCuNVMxPjRizszN NXPoZpV6W0GQR1XS
MEL-JUSO NWK0fYE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTBwMUOxNlgh|ryP Ml7SV2FPT0WU
P30-OHK MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\VTohKSzVyPUCuNVMyPiEQvF2= NW[zfpRXW0GQR1XS
CTB-1 NGT0T3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTBwMUOzNlUh|ryP M3XHdXNCVkeHUh?=
ACHN NUKzU|hFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DL[GlEPTB;MD6xN|M3QSEQvF2= MXzTRW5ITVJ?
ES5 Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3e3RWlEPTB;MD6xN|Q4PiEQvF2= NXXFfoUxW0GQR1XS
SF539 NH;IS29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfwNVlDUUN3ME2wMlE{PjhizszN NVjaVW5OW0GQR1XS
D-283MED MorKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXvTWM2OD1yLkGzOlg4KM7:TR?= MXfTRW5ITVJ?
OVCAR-5 NV;ufnZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfqTWM2OD1yLkGzPFk1KM7:TR?= M2rnVXNCVkeHUh?=
RPMI-8866 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTacYpKSzVyPUCuNVM6OjVizszN MlTFV2FPT0WU
PANC-03-27 NXe1e2lkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnUfIpGUUN3ME2wMlE1Ojh6IN88US=> NXLy[3V4W0GQR1XS
M14 NY[2OWVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7RRVZiUUN3ME2wMlE1PDZ|IN88US=> M{m3fXNCVkeHUh?=
D-566MG Ml\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;4eGlEPTB;MD6xOFY6KM7:TR?= M3HoTXNCVkeHUh?=
Ramos-2G6-4C10 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rnR2lEPTB;MD6xOFg1OiEQvF2= M1;2T3NCVkeHUh?=
NOS-1 M2jJ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDkTWM2OD1yLkG0PVY4KM7:TR?= NWr4cG1HW0GQR1XS
YH-13 NIPCO2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7hWlA5UUN3ME2wMlE2Pjd2IN88US=> NFHQUXNUSU6JRWK=
NB17 NVmy[pFlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHoZY1KUUN3ME2wMlE2Pzh4IN88US=> Ml;sV2FPT0WU
U-2-OS NW\FeWV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfCbnJKSzVyPUCuNVU5PDdizszN NILqZpBUSU6JRWK=
ES7 NILSTWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vsRWlEPTB;MD6xOlAyOSEQvF2= MVjTRW5ITVJ?
LoVo MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwMU[xOlYh|ryP NV;2VIg2W0GQR1XS
QIMR-WIL Ml7aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTBwMU[zNVEh|ryP MYXTRW5ITVJ?
ESS-1 NEPzcYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTBwMU[0NVMh|ryP MUTTRW5ITVJ?
LAMA-84 MkT2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrkTWM2OD1yLkG2OVA{KM7:TR?= NFzFVmtUSU6JRWK=
TGBC24TKB NEnnVlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPEVWlKSzVyPUCuNVY2OzVizszN NGrUR|hUSU6JRWK=
KU812 NIfmRoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTBwMU[4N{DPxE1? NVj1e3liW0GQR1XS
KARPAS-45 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn6xTWM2OD1yLkG2PFQ4KM7:TR?= NUPSfHc1W0GQR1XS
ACN NUXyW5dwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXmTWM2OD1yLkG3NVcyKM7:TR?= MmPvV2FPT0WU
RPMI-8226 M1L3bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTkTWM2OD1yLkG3OFY1KM7:TR?= NHHmPINUSU6JRWK=
GR-ST NY\IWIplT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHjdI9PUUN3ME2wMlE4QDJ5IN88US=> MV\TRW5ITVJ?
SK-UT-1 NWjlSWJ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwMUi1PVkh|ryP MoDYV2FPT0WU
SK-N-AS MmjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmjMTWM2OD1yLkG4PFE2KM7:TR?= NGfwOlVUSU6JRWK=
EW-1 MmG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2ewVGlEPTB;MD6xPFkxPSEQvF2= NF7QephUSU6JRWK=
5637 NFO4RWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4G5emlEPTB;MD6xPVI4QCEQvF2= MmC2V2FPT0WU
GI-1 NFftToRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mon3TWM2OD1yLkG5OVYyKM7:TR?= MXjTRW5ITVJ?
NCI-H1395 NELPdIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnkWGxKSzVyPUCuNVk4PjFizszN NILYW2RUSU6JRWK=
CHP-212 Mkn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XP[WlEPTB;MD6xPVc5OiEQvF2= MoDnV2FPT0WU
A427 NH;PN2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjxc3RKSzVyPUCuNVk5OzJizszN M1rTO3NCVkeHUh?=
BE-13 NVXUcpZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPsVmw5UUN3ME2wMlIxOTF5IN88US=> NYq0T5p6W0GQR1XS
RS4-11 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\OO5dKSzVyPUCuNlA{OThizszN NUT0VJNXW0GQR1XS
TI-73 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33z[2lEPTB;MD6yNFg4OyEQvF2= NGjMcGJUSU6JRWK=
HAL-01 MoLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfjTWM2OD1yLkKwPVch|ryP MUfTRW5ITVJ?
KY821 NEHNRopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrQTWM2OD1yLkKxOFI6KM7:TR?= MYTTRW5ITVJ?
H4 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHiZWNKSzVyPUCuNlIxPjJizszN M3\WZ3NCVkeHUh?=
EPLC-272H NH3uTVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnNbFNKSzVyPUCuNlIxPjhizszN MVfTRW5ITVJ?
COR-L105 NYr4cFFsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwMkKyN|Uh|ryP NFHPV21USU6JRWK=
769-P MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LWWGlEPTB;MD6yNlI6QCEQvF2= NEjvVFJUSU6JRWK=
HEL NXnCZVMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTBwMkK0OVQh|ryP NYDZRoNvW0GQR1XS
VA-ES-BJ NF3rPYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2j6SWlEPTB;MD6yNlU2PyEQvF2= NX7lZY43W0GQR1XS
IST-MEL1 NGXFSG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnzb3RKSzVyPUCuNlI2PyEQvF2= M3iw[XNCVkeHUh?=
KYSE-270 M2TZOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTBwMkK2N|Mh|ryP M4jJXnNCVkeHUh?=
RH-1 Mlf5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVqyfI9YUUN3ME2wMlIzPjZ4IN88US=> Mo\SV2FPT0WU
JVM-3 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwMkOwN|Yh|ryP M2TXUnNCVkeHUh?=
RPMI-7951 NUC5U2hiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\RR|Y4UUN3ME2wMlI{ODV4IN88US=> MmezV2FPT0WU
JAR NXvvO|FiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PZU2lEPTB;MD6yN|I{QCEQvF2= Moi1V2FPT0WU
MOLT-4 MoGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTBwMkO4OUDPxE1? M{XNWXNCVkeHUh?=
ALL-PO MmruS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3aTWM2OD1yLkK0NlQ{KM7:TR?= NGjm[I1USU6JRWK=
SNU-387 M{S4N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEm2NIZKSzVyPUCuNlQ{OzlizszN NHX1b4pUSU6JRWK=
IGROV-1 MmHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1G4SmlEPTB;MD6yOFQxOSEQvF2= NV\KW3lJW0GQR1XS
MPP-89 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;XRm9HUUN3ME2wMlI1PDVzIN88US=> NFSwZYlUSU6JRWK=
T98G Mn7VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljLTWM2OD1yLkK0OVY5KM7:TR?= MWDTRW5ITVJ?
NCI-H510A MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHz4c5pKSzVyPUCuNlQ3OjZizszN M4jxTnNCVkeHUh?=
KOSC-2 NGi0dllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwMkS2O|Eh|ryP NVjIN3lTW0GQR1XS
NCI-H23 NV\kVHFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HxN2lEPTB;MD6yOFc3OSEQvF2= NYnSdG9oW0GQR1XS
MC116 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3:2NmlEPTB;MD6yOVY2PCEQvF2= M1HlW3NCVkeHUh?=
8-MG-BA Mkf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\3TGlEPTB;MD6yOVc3QCEQvF2= MYjTRW5ITVJ?
ABC-1 NVTQdmVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\X[5hKSzVyPUCuNlYxPjRizszN NYHNTnZRW0GQR1XS
J-RT3-T3-5 NGnH[49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIO3OJdKSzVyPUCuNlY3PzhizszN M{TocHNCVkeHUh?=
NCI-SNU-1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnaTWM2OD1yLkK3NFU6KM7:TR?= NX76XWtqW0GQR1XS
HCC1395 M3;pemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1KxOmlEPTB;MD6yO|A5QSEQvF2= M{\QS3NCVkeHUh?=
BB65-RCC MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDqZZhSUUN3ME2wMlI4ODl5IN88US=> NXy5TVNYW0GQR1XS
NCI-H1755 NXfhe5dZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLW[lFKSzVyPUCuNlc{ODRizszN NHvGU|NUSU6JRWK=
OVCAR-3 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTBwMkezPFUh|ryP NHLkbWxUSU6JRWK=
MKN45 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTBwMke5PEDPxE1? MoLiV2FPT0WU
TYK-nu NWr4RoM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrqOmpSUUN3ME2wMlI5ODZizszN NHXCXWVUSU6JRWK=
SNU-C2B NETu[5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnz2TWM2OD1yLkK4NVM1KM7:TR?= NELtTZdUSU6JRWK=
H-EMC-SS MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLiTWM2OD1yLkK4N|U1KM7:TR?= NXvkOIp3W0GQR1XS
CAKI-1 M3;iWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Moj1TWM2OD1yLkK4Olgh|ryP MV3TRW5ITVJ?
EW-24 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmraTWM2OD1yLkK4PVEh|ryP NIHtVXVUSU6JRWK=
RXF393 NGj2cnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWm5TIN{UUN3ME2wMlI6ODB6IN88US=> NUjPcGF6W0GQR1XS
CCRF-CEM NWXHVmFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3izeWlEPTB;MD6yPVE2PCEQvF2= M3\OOXNCVkeHUh?=
HOP-92 NF3vNY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fiSGlEPTB;MD6yPVI4QSEQvF2= NXLvWJR3W0GQR1XS
COLO-684 NEXMdW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1myUmlEPTB;MD6yPVQxPiEQvF2= NYXOOHd3W0GQR1XS
NCI-H28 M3i1d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzpOpNKSzVyPUCuNlk4ODRizszN M4nzTnNCVkeHUh?=
HuO9 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXH[Vl{UUN3ME2wMlI6Pzl|IN88US=> MmPzV2FPT0WU
SF295 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDYPWRKSzVyPUCuNlk5ODJizszN M{nRdXNCVkeHUh?=
COLO-829 NXexOYVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrDTYFrUUN3ME2wMlI6QDR3IN88US=> M1zE[nNCVkeHUh?=
MSTO-211H NUTYSnEzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jLUGlEPTB;MD6yPVg2PSEQvF2= NWH4T3NEW0GQR1XS
MDA-MB-361 NWHKbWN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\RZndKSzVyPUCuNlk5QTZizszN MlLmV2FPT0WU
FTC-133 NYXtfGQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjXTWM2OD1yLkOwNlY2KM7:TR?= Mlz6V2FPT0WU
Mewo MmHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwM{C0N|Eh|ryP NGmyXYJUSU6JRWK=
HCC70 Ml:5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXMTWM2OD1yLkOwOlY2KM7:TR?= NHHsZXBUSU6JRWK=
BB30-HNC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vlbGlEPTB;MD6zNVUzPSEQvF2= M3j6TnNCVkeHUh?=
LCLC-97TM1 NHru[FZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjrTWM2OD1yLkOxOVY3KM7:TR?= Mn70V2FPT0WU
HCC2998 MkLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3UTWM2OD1yLkOxOlUh|ryP NIPoUoVUSU6JRWK=
NBsusSR M3fnVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnH3TWM2OD1yLkOxPFE6KM7:TR?= M2HPTnNCVkeHUh?=
OVCAR-4 MlTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[yTWM2OD1yLkOyNFIyKM7:TR?= M3PQ[3NCVkeHUh?=
NCI-H209 M{Pxbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETDW4xKSzVyPUCuN|I{PTNizszN MnexV2FPT0WU
ChaGo-K-1 NUC0UXh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHYeoV6UUN3ME2wMlMzPDh7IN88US=> Mle0V2FPT0WU
KALS-1 NFrpOYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XLeGlEPTB;MD6zNlU6PCEQvF2= NX\TfVczW0GQR1XS
D-423MG NILo[IlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrRTWM2OD1yLkOzNVQzKM7:TR?= Ml7BV2FPT0WU
Detroit562 NXvKfmluT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2f0WWlEPTB;MD6zN|M1OyEQvF2= NHXLToVUSU6JRWK=
HT-144 NF7WPWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV76WnpnUUN3ME2wMlM{QDFizszN MofUV2FPT0WU
MEG-01 NEDjVndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLXd2RDUUN3ME2wMlM1PTh2IN88US=> MXzTRW5ITVJ?
BV-173 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnC[HpKSzVyPUCuN|Q2QTZizszN MX3TRW5ITVJ?
MZ7-mel MmW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPaTWM2OD1yLkO0Olc4KM7:TR?= MYDTRW5ITVJ?
C32 M4LsR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHmTYhKSzVyPUCuN|Q3QDJizszN NHu0SFlUSU6JRWK=
DU-4475 MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUe3fXB3UUN3ME2wMlM1QDd3IN88US=> NW\EXpczW0GQR1XS
KP-N-YN MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\ZUGlEPTB;MD6zOFg5PyEQvF2= NF;VZWlUSU6JRWK=
MKN1 NFLaTZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmGzTWM2OD1yLkO1NFAzKM7:TR?= MWfTRW5ITVJ?
8505C M1rKVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTBwM{WwNFIh|ryP MlTwV2FPT0WU
OVCAR-8 NEfRUnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYL2c4RqUUN3ME2wMlM2PTh2IN88US=> M3\tUHNCVkeHUh?=
NH-12 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTBwM{W2PVYh|ryP MmDiV2FPT0WU
COR-L88 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjUTWM2OD1yLkO2OFgzKM7:TR?= M1SzNnNCVkeHUh?=
T-24 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTBwM{[5PFUh|ryP MV\TRW5ITVJ?
MIA-PaCa-2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3IPFRrUUN3ME2wMlM4PDl5IN88US=> M2TPO3NCVkeHUh?=
LS-411N MmHSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX6wTFRSUUN3ME2wMlM4PjB4IN88US=> NY\rfJpzW0GQR1XS
TK10 MljZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnlSotKSzVyPUCuN|c6PDhizszN Mlm3V2FPT0WU
SW1990 M4HGb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XWbmlEPTB;MD6zPFM2OyEQvF2= Mly3V2FPT0WU
GAMG NXfXXo9YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2m2Z2lEPTB;MD6zPFU3PyEQvF2= NFrJO|dUSU6JRWK=
G-361 NVXIZoEzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPBTWM2OD1yLkO4O|EzKM7:TR?= Mlf1V2FPT0WU
Ca-Ski MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwM{i3PVIh|ryP MUPTRW5ITVJ?
786-0 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfmTWM2OD1yLkO4PFI{KM7:TR?= M1TFcXNCVkeHUh?=
MHH-NB-11 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwM{i5O{DPxE1? MWfTRW5ITVJ?
OE33 M1nmUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUP0eWhCUUN3ME2wMlM6OTF6IN88US=> MlT5V2FPT0WU
SW954 NGf3NZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTMUlFbUUN3ME2wMlQxOTNzIN88US=> NWrveJVsW0GQR1XS
KYSE-180 MmXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjYdoV7UUN3ME2wMlQxOTVzIN88US=> NYnNXZNrW0GQR1XS
NCI-H720 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3H1NWlEPTB;MD60NFE6OSEQvF2= NV3WNIkyW0GQR1XS
COR-L23 NWSyXHFJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DFNGlEPTB;MD60NFI5PiEQvF2= Ml\FV2FPT0WU
PFSK-1 MkTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXH4ZndjUUN3ME2wMlQyODl{IN88US=> MWDTRW5ITVJ?
FADU NVPI[4E6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1KyXmlEPTB;MD60NVE4OiEQvF2= MWrTRW5ITVJ?
GP5d M1L6dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17qXGlEPTB;MD60NVIzPSEQvF2= M4iwSXNCVkeHUh?=
PC-14 M3TKRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mkj6TWM2OD1yLkSxOlYyKM7:TR?= NUDUUZJpW0GQR1XS
SJSA-1 MoHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvvcHFKSzVyPUCuOFE4OTRizszN Mn3nV2FPT0WU
D-336MG MnjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrtSYNvUUN3ME2wMlQzOTN6IN88US=> M3zPb3NCVkeHUh?=
LOXIMVI NY\YPVJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTBwNEK4PFUh|ryP MkDhV2FPT0WU
EFO-27 M2rkWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTBwNEOwOFkh|ryP NWDsTpRIW0GQR1XS
A2780 MkTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTBwNEOxOlIh|ryP MX;TRW5ITVJ?
CAL-12T MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDTcIpGUUN3ME2wMlQ{Ojh6IN88US=> NHT1[4RUSU6JRWK=
ES6 NHXIfnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvDTWM2OD1yLkS0NVg2KM7:TR?= MVHTRW5ITVJ?
HCE-T M370bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwNESxPFch|ryP M1\QUHNCVkeHUh?=
SK-MES-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVO4XY5IUUN3ME2wMlQ1Ojl|IN88US=> NGrz[IRUSU6JRWK=
Daoy Mn\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XpVmlEPTB;MD60OVExOyEQvF2= MXnTRW5ITVJ?
A2058 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nuWWlEPTB;MD60OVE{PiEQvF2= NFO0NWJUSU6JRWK=
Hs-578-T NFjae4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmC1TWM2OD1yLkS1Nlc5KM7:TR?= M2rTenNCVkeHUh?=
TE-11 NX\rPZBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTBwNEWyPVMh|ryP NUTZUpM{W0GQR1XS
GI-ME-N NYXHW3BtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPzem0yUUN3ME2wMlQ2PDZizszN NELvdmdUSU6JRWK=
D-392MG NH7hTIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LF[WlEPTB;MD60OVYyOiEQvF2= NX;rT4NwW0GQR1XS
MOLT-13 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\0TWM2OD1yLkS2Nlk1KM7:TR?= MoDYV2FPT0WU
SK-NEP-1 MonDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r3T2lEPTB;MD60OlQyQSEQvF2= NYriR4p4W0GQR1XS
KS-1 NHX5VHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrmS49GUUN3ME2wMlQ3PjB|IN88US=> MY\TRW5ITVJ?
HuP-T4 NITtZ5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjRT21QUUN3ME2wMlQ3QDZ6IN88US=> NUfxfJlrW0GQR1XS
NCI-H1563 M135VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTBwNEewN|Uh|ryP MnzTV2FPT0WU
U031 NIXRT5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTBwNEiyN|Qh|ryP MnrmV2FPT0WU
HSC-3 M{LDVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnBTWM2OD1yLkS4OlI3KM7:TR?= M4\Hc3NCVkeHUh?=
HT-3 NIG1S|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{H5TmlEPTB;MD60PVA{KM7:TR?= NUHCdpNRW0GQR1XS
HGC-27 M2f2VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTBwNEmxNVUh|ryP M4jFcnNCVkeHUh?=
HT-1080 NVXRSpIzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7HTWM2OD1yLkS5Nlch|ryP MlG3V2FPT0WU
RT-112 NIiwSYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwNEm5NlEh|ryP MXrTRW5ITVJ?
A375 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTBwNEm5NlIh|ryP MWHTRW5ITVJ?
SW626 NGTT[|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7DZYpEUUN3ME2wMlUxPjN|IN88US=> MYrTRW5ITVJ?
SBC-5 NGjCb3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHJTWM2OD1yLkWxN|Q1KM7:TR?= NFf4R3RUSU6JRWK=
CAL-51 MljES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\HZ2lEPTB;MD61NVQ{PyEQvF2= NVXrT2FbW0GQR1XS
RKO NYDtcolsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rKW2lEPTB;MD61NlAyQSEQvF2= MlvoV2FPT0WU
HC-1 NWLVd3pFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTBwNUKxNVQh|ryP Mo\XV2FPT0WU
CHL-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HBbWlEPTB;MD61NlU2OSEQvF2= MVzTRW5ITVJ?
SK-N-DZ M{XrXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmD4TWM2OD1yLkWzNFc5KM7:TR?= NG\3[2VUSU6JRWK=
SNU-423 NVS1V455T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGf5VWdKSzVyPUCuOVM1PjZizszN M2Xhd3NCVkeHUh?=
NB13 NVTLZlRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlqxTWM2OD1yLkWzOlU4KM7:TR?= MVHTRW5ITVJ?
EW-16 M{HTXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTBwNUO3O|Qh|ryP MYnTRW5ITVJ?
A704 NEHNSHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jPZmlEPTB;MD61N|g5OSEQvF2= NGHz[oJUSU6JRWK=
Ca9-22 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjMSnpKSzVyPUCuOVQxPzdizszN Ml3uV2FPT0WU
NCI-H1299 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTBwNUW1PEDPxE1? NH[5fnhUSU6JRWK=
LB771-HNC NXuyOZpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWG5R2R5UUN3ME2wMlU2QTN|IN88US=> Mn\yV2FPT0WU
EW-11 NF\HSnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;HNGlqUUN3ME2wMlU3OzJ4IN88US=> NWnMdVhCW0GQR1XS
LC-2-ad MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwNU[1NlIh|ryP NWfuVIxwW0GQR1XS
KNS-62 NYXBRmQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1THXGlEPTB;MD61OlU2OyEQvF2= MoTiV2FPT0WU
CAL-62 NEfId2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLrc4xKSzVyPUCuOVcyOjFizszN MXrTRW5ITVJ?
SF126 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIrzTIdKSzVyPUCuOVc1OjFizszN NIH3NW1USU6JRWK=
OMC-1 NYnzTo9pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jQeGlEPTB;MD61O|Q6PyEQvF2= MoLMV2FPT0WU
AGS MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwNUixOlIh|ryP MYTTRW5ITVJ?
EW-22 NEX3ZZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTBwNUizO|Uh|ryP MkO0V2FPT0WU
KP-N-YS MlPKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrWWWVKSzVyPUCuOVg{QDJizszN MYfTRW5ITVJ?
BFTC-909 NW\QZlFFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHS5bGpKSzVyPUCuOVg5OzNizszN NEfpSVhUSU6JRWK=
PSN1 M3m2PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvxWW5GUUN3ME2wMlU6OTF6IN88US=> MX\TRW5ITVJ?
NB10 M3TER2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnSeGpKSzVyPUCuOVkyOjlizszN Ml7yV2FPT0WU
COLO-800 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\oTWM2OD1yLk[wN|I5KM7:TR?= M4jWPXNCVkeHUh?=
647-V NHG0c3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTBwNkG3PFch|ryP M1TE[3NCVkeHUh?=
VM-CUB-1 M3Tydmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nnXGlEPTB;MD62NVg3OSEQvF2= NGrldJlUSU6JRWK=
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NCI-H226 MmPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjONIxKSzVyPUSuOVQ5PzFizszN MX7TRW5ITVJ?
CAL-27 MkXMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHj3OoxKSzVyPUSuO|E3QTRizszN MXjTRW5ITVJ?
G-401 NVzkS2F3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{SxS2lEPTB;ND64N|g1OSEQvF2= NGPpZXZUSU6JRWK=
CAL-120 NEH6VI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nyeWlEPTB;ND64OFk6OSEQvF2= NWHJNYR5W0GQR1XS
DMS-114 NWTycppbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TaUmlEPTB;NT6xOFUxPiEQvF2= M{jWeHNCVkeHUh?=
KYSE-70 M4POZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlfZTWM2OD13LkG0PFU6KM7:TR?= M2TkR3NCVkeHUh?=
NCI-H1648 M1XTSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7jTIE2UUN3ME21MlI6Pzh{IN88US=> NXLybYw4W0GQR1XS
MDA-MB-175-VII NVS4OIpPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\De3JZUUN3ME21MlQ1ODF2IN88US=> Mmf3V2FPT0WU
NCI-H2029 Mm\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILOVIJKSzVyPUWuOFgxOjJizszN NHXUc2FUSU6JRWK=
LS-1034 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\udndlUUN3ME21MlY3QDh7IN88US=> NUPNXG85W0GQR1XS
LB831-BLC MmS3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTVwN{CzOFUh|ryP NX\rXpNIW0GQR1XS
MDA-MB-415 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moj2TWM2OD13LkewPFEh|ryP MkHXV2FPT0WU
TE-6 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\XTWM2OD13Lke0NVgzKM7:TR?= NYPoNYdrW0GQR1XS
SK-MEL-24 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTVwN{S1OlQh|ryP NInzeolUSU6JRWK=
HUTU-80 NVK1[mxIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH34WpBKSzVyPUWuO|U{OzNizszN MV\TRW5ITVJ?
OCUB-M M2jmbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTVwN{e1OlMh|ryP MlfYV2FPT0WU
IGR-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjJTWM2OD13Lki2PFk1KM7:TR?= NVq1S416W0GQR1XS
NCI-H747 NWHGWXJHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4OxNmlEPTB;Nj6yOFU5PSEQvF2= Mk\YV2FPT0WU
COLO-680N MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K1S2lEPTB;Nj6zPVQxOyEQvF2= NXPzVHd3W0GQR1XS
NB12 M3PzR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVThS3N4UUN3ME22Mlc6PzJ{IN88US=> MmC2V2FPT0WU
SNU-449 MonqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTZwOUm1PVEh|ryP Mmm1V2FPT0WU
D-542MG NYLPVlRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2j4OmlEPTB;Nz6xNlI6QSEQvF2= MXfTRW5ITVJ?
COLO-792 NIHacJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LRNGlEPTB;Nz64O|EzPyEQvF2= MYrTRW5ITVJ?
NCI-H810 M1vvR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;BTWM2OD16LkCyPVg1KM7:TR?= NI\EcIpUSU6JRWK=
T47D NUfoenFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRThwMU[wNlQh|ryP NXTtUZpSW0GQR1XS
Calu-3 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjufIhlUUN3ME24MlI5QDh4IN88US=> Mn:zV2FPT0WU
COLO-741 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRThwOUOwPVMh|ryP NYLO[GU4W0GQR1XS
LU-135 NEXZT3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvafnVKSzVyPUmuNFM1PTJizszN NXXQ[5V2W0GQR1XS
COLO-678 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2r3bGlEPTB;OT6yPFg1PCEQvF2= Mm\VV2FPT0WU
NCI-H2126 MlHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnlbItKSzVyPUmuN|M6OTlizszN MX;TRW5ITVJ?
SK-MEL-28 NIjqWGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWH3bZlWUUN3ME25MlU{PTl4IN88US=> NEDUT4JUSU6JRWK=
MFE-280 M{LKW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XGeWlEPTB;MUCuNFIzQSEQvF2= NYr5Npd4W0GQR1XS
DBTRG-05MG NEnjdJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\YTWM2OD1zMD60N|Y6KM7:TR?= NUXNU5ZRW0GQR1XS
RCC10RGB MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnJTWM2OD1zMD61NlU2KM7:TR?= MkPnV2FPT0WU
NTERA-S-cl-D1 NG\oT5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTFyLk[4OVIh|ryP MWXTRW5ITVJ?
C3A NIj3[I9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjHSo9JUUN3ME2xNk4{OTd{IN88US=> MVLTRW5ITVJ?
MHH-ES-1 MkK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3jWYFKSzVyPUGyMlUzQDRizszN NXf0TYNjW0GQR1XS
LU-139 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTF{Lk[zN|Mh|ryP NYXGT3hRW0GQR1XS
KNS-42 M1vSVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LZWmlEPTB;MUKuPFc4OSEQvF2= NEjYUplUSU6JRWK=
MCF7 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljJTWM2OD1zMz6yN|Y2KM7:TR?= NUXzdHNUW0GQR1XS
SW872 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGL2UWVKSzVyPUG0MlU1PjFizszN NHvoOJNUSU6JRWK=
HT-1376 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnxdlFKSzVyPUG0Mlg2QDJizszN MVPTRW5ITVJ?
NCI-SNU-5 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4qzNGlEPTB;MUWuNVc1OSEQvF2= M3y2NXNCVkeHUh?=
NCI-H2405 MkD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjEWnRiUUN3ME2xOU41PDd2IN88US=> M1nTT3NCVkeHUh?=
KYSE-150 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnNTWM2OD1zNT63OlEyKM7:TR?= NVH2UXpWW0GQR1XS
SW837 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PhbGlEPTB;MU[uNFEzPCEQvF2= M4nqWXNCVkeHUh?=
LU-65 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;ETmlEPTB;MUmuO|I3KM7:TR?= M4LMcHNCVkeHUh?=
JEG-3 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYqzfodmUUN3ME2yNE4{PTd5IN88US=> NGGxNWRUSU6JRWK=
SK-MEL-1 NHfrV|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\tTWM2OD1{MD63PFM3KM7:TR?= M2jjRXNCVkeHUh?=
SK-HEP-1 M1T4OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojpTWM2OD1{MT61OVQyKM7:TR?= M1LF[XNCVkeHUh?=
NCI-H1770 M{j0T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWG5XVRPUUN3ME2yN{41ODh4IN88US=> M33pXXNCVkeHUh?=
MG-63 M3fIdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJ|Lke2O|Mh|ryP M13JbnNCVkeHUh?=
ECC10 NH3RRYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHyTWM2OD1{NT61O|Eh|ryP MYnTRW5ITVJ?
SW1463 NGjIWoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7BWmZMUUN3ME2yO{45OzF4IN88US=> M3LEZnNCVkeHUh?=
JVM-2 NHrRbmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXmyXpY2UUN3ME2yPE4{OjR6IN88US=> NEn6dpRUSU6JRWK=
HCT-15 NFi4W3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M373TGlEPTB;MkiuOFE6OiEQvF2= Mn\iV2FPT0WU
CAL-72 M1yy[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTNzLkS5NVIh|ryP NWnvPJZOW0GQR1XS
HCC2218 NF\mVXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvRPFBKSzVyPUOyMlg5PThizszN NXH2SIE6W0GQR1XS
BT-474 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fE[mlEPTB;M{OuNFg1OyEQvF2= Ml3xV2FPT0WU
NCI-H1092 M2n5[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHFe2ZpUUN3ME2zN{42OTR3IN88US=> M3\EbXNCVkeHUh?=
Mo-T MoDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfRRlRKSzVyPUO3MlM2QDVizszN Ml\oV2FPT0WU
DU-145 NXqwU5NOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkm4TWM2OD12MD62Nlk2KM7:TR?= M4rMSXNCVkeHUh?=
NCI-H1666 MnW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrONmxRUUN3ME20Nk41PDh6IN88US=> MXfTRW5ITVJ?
IST-MES1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXoTWM2OD12Nj60NVk3KM7:TR?= M{XvOnNCVkeHUh?=
SW1116 NGjUc4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn64TWM2OD12OD6xO|U3KM7:TR?= MVHTRW5ITVJ?
MZ2-MEL M3fFcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLaTWM2OD12OD61NFI3KM7:TR?= MlfRV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pChk1 / Chk1 / Wee1 / p21 / p53 ; 

PubMed: 29963769     


Immunoblotting analysis of CAL39 and SW954 cells showing increase in CHK1 phosphorylation after exposure to AZD7762 for 24 hours. β‐actin is used as loading control. Representative data from n = 3 experiments.

29963769
Growth inhibition assay
Cell viability; 

PubMed: 23715154     


Lkb1-wt (634, 855, and 857) and Lkb1-null (t2, t4, and t5) cell lines were plated into 96-well plates at 2000 cells/well in 150 ml medium containing the indicated concentrations of AZD7762 or CHIR124 for 3 days. Viable cells were then counted with Dojindo’s Cell Counting Kit-8 assay. The data represent mean ± SD for 3 repeats. GI50 was calculated with GraphPad.

23715154
In vivo AZD7762 alone at 25 mg/kg shows little antitumor activity in the H460-DNp53 xenograft mice and SW620 xenograft mice, but when administrated in combination with gemcitabine (60 mg/kg), AZD7762 shows significant antitumor efficacy in the two xenografts mice with a log cell kill of 0.9 or percent treated/control (%T/C) of 26 even at low dose of 12.5 mg. Dosing of AZD7762 in combination with gemcitabine (10 mg/kg) in the H460-DNp53 xenograft rat inhibits the tumor volume in a dose-dependent manner with the %T/C values of 48 and 32 for 10 and 20 mg/kg AZD7762, respectively. AZD7762 (25 mg/kg) in combination with irinotecan (25 or 50 mg/kg) causes complete tumor regression in the SW620 xenograft mice with the %T/C increasing significantly to -66% and -67%, respectively. [1]

Protocol

Kinase Assay:[1]
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Chk1 Kinase Assay:

Recombinant human Chk1 is expressed as a glutathione S-transferase fusion in insect cells using a baculovirus vector and purified by glutathione affinity chromatography. A synthetic peptide substrate (N-biotinylaminohexanoyl-KKVSRSGLYRSPMPENLNRPR) for Chk1 is synthesized. Final assay concentrations of peptide and ATP (cold + 40 nCi [33P]ATP) are 0.8 and 1 μM, respectively. Different concentrations of AZD7762, buffer containing peptide and chk1 kinase and ATP, are added sequentially to a 384-well assay plate. The plate is incubated for 2 hours, reaction is stopped by the addition of buffer containing EDTA and scintillation proximity assay beads, and plates are read using a TopCount reader. Data analysis is carried out to determinate a dose response (IC50).
Cell Research:[1]
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  • Cell lines: HT29, SW620 and MDA-MB-231 cells
  • Concentrations: Dissolved in DMSO, final concentration ~12.5 μM
  • Incubation Time: 20 or 48 hours
  • Method: For the checkpoint abrogation assay, HT29 cells are treated for 2 hours with camptothecin (topoisomerase I inhibitor; 0.07 μg/mL) to induce the G2 checkpoint. Cells are then treated for 20 hours with a 12-point titration of AZD7762 (12.5 μM to 6 nM) plus nocodazole. Cells are fixed with 3.7% formaldehyde for 1 hour, permeabilized with PBS containing 0.05% Triton X, and incubated with anti-phH3 antibody for 1 hour followed by Alexa Fluor 488 anti-rabbit and Hoechst stain for 1 hour. Mitotic index is determined on the ArrayScan and expressed as the percentage of cells undergoing mitosis. For the potentiation assays, SW620 or MDA-MB-231 cells are dosed for 24 hours with a 9-point titration of gemcitabine ranging from 0.01 to 100 nM with a constant dose of AZD7762 (300 nM). After 24 hours, medium is removed and AZD7762 alone is added back to the wells for an additional 24 hours. Cells are then incubated in AZD7762-free medium for an additional 72 hours. The effect on cell proliferation is determined by MTT.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Male NCr mice implanted s.c. with H460-DNp53 cells or SW620, and male rnu rats implanted s.c. with H460-DNp53 cells
  • Dosages: ~25 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (137.96 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.42
Formula

C17H19FN4O2S

CAS No. 860352-01-8
Storage powder
in solvent
Synonyms N/A
Smiles C1CC(CNC1)NC(=O)C2=C(C=C(S2)C3=CC(=CC=C3)F)NC(=O)N

In vivo Formulation Calculator (Clear solution)

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    If I want to use the inhibitor for i.p. injection, can I use DMSO to dissolve it?

  • Answer:

    Since DMSO has low toxicity with i.p. injection, hydroxyproplyl-β-cyclodextrin is consider a safer co-solvent in compound formulation. In a previous literature report, 11.3% hydroxyproplyl-β-cyclodextrin was used to formulate this compound for i.v. injection.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID