AZD7762

For research use only.

Catalog No.S1532

80 publications

AZD7762 Chemical Structure

CAS No. 860352-01-8

AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.

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Selleck's AZD7762 has been cited by 80 publications

Purity & Quality Control

Choose Selective Chk Inhibitors

Biological Activity

Description AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.
Targets
Chk1 [1]
(Cell-free assay)
Chk2 [1]
(Cell-free assay)
5 nM <10 nM
In vitro

AZD7762, a more selective Chk1 inhibitor, inhibits Chk1 phosphorylation of a cdc25C peptide by reversibly binding to the ATP-binding site of Chk1, with IC50 of 5 nM and Ki of 3.6 nM. AZD7762 induces cell arrest with EC50 of 0.620 μM, and significantly abrogates the camptothecin induced G2 arrest with an EC50 of 10 nM, by blocking the chk1 dependent degradation of Cdc25A and activation of Cyclin A. AZD7762 (300 nM) enhances the antitumor efficacy of gemcitabine against SW620 and topotecan against MDA-MB-231 by reducing the GI50 values from 24.1 nM and 2.25 μM to 1.08 nM and 0.15 μM, respectively. [1] AZD7762 shows cytotoxicity against a variety of neuroblastoma cell lines bearing p53 wild type, p53 mutation, Mdm2 amplification or p14 deletion with IC50 values ranging from 82.6-505.9 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KASUMI-1 M{nJ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TQVmlEPTB;MD6wNFAzOzRizszN NVLoXohmW0GQR1XS
NKM-1 Ml7IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnP4TWM2OD1yLkCwNFQyPSEQvF2= NXGxPFVsW0GQR1XS
MV-4-11 M13kN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjLWHpyUUN3ME2wMlAxODR{NTFOwG0> MnTtV2FPT0WU
NCI-H1703 NVjx[IZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoH2TWM2OD1yLkCwOlQ{KM7:TR?= NU\EZYN2W0GQR1XS
A204 NV7RclRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk[wTWM2OD1yLkCwPFU3KM7:TR?= MnTOV2FPT0WU
CGTH-W-1 M3u4N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrEPVVbUUN3ME2wMlAyPDJizszN NU\LbGF6W0GQR1XS
MLMA MkOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWr1XFRXUUN3ME2wMlAzQDR7IN88US=> MYLTRW5ITVJ?
GOTO M{fOdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlW5TWM2OD1yLkCzNVIh|ryP M2fjSHNCVkeHUh?=
KE-37 M{C5NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrYUJRKSzVyPUCuNFMyPDJizszN MY\TRW5ITVJ?
ES4 M4fTcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTuNXk2UUN3ME2wMlA{OjVzIN88US=> MlTMV2FPT0WU
ATN-1 NFnoepVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknJTWM2OD1yLkCzOFE4KM7:TR?= NF;rWo1USU6JRWK=
MHH-PREB-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHVNolKSzVyPUCuNFM1PDJizszN M2rWVnNCVkeHUh?=
SW756 M4f2dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLvdZNKSzVyPUCuNFM2PzlizszN MnL0V2FPT0WU
CTV-1 NYrVbHpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTBwMEO4PVYh|ryP M3jCS3NCVkeHUh?=
HCC1806 NWDQNGZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTBwMESwNlIh|ryP MkjEV2FPT0WU
NEC8 M3nXV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILjcIZKSzVyPUCuNFQzODNizszN NUf0Rm12W0GQR1XS
P12-ICHIKAWA NXXsUXJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLjWYdKSzVyPUCuNFQ{OTZizszN MYfTRW5ITVJ?
DEL M2jWW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfmUnJKSzVyPUCuNFQ2QTJizszN NGrEN|BUSU6JRWK=
KM12 NUXLO3E{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4X5e2lEPTB;MD6wOVE2QCEQvF2= MYjTRW5ITVJ?
LB1047-RCC MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnETWM2OD1yLkC2NFI2KM7:TR?= MXLTRW5ITVJ?
LB2241-RCC M1vBRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPTcHVmUUN3ME2wMlA3OTB{IN88US=> MljDV2FPT0WU
NOMO-1 NIfWcZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDLTWM2OD1yLkC2NVE2KM7:TR?= NEjwcGJUSU6JRWK=
NB14 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\jb2lEPTB;MD6wOlE{PiEQvF2= NUXhcm9IW0GQR1XS
ONS-76 NHniPItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NET2VVZKSzVyPUCuNFYyQTZizszN NI[4NHhUSU6JRWK=
HD-MY-Z NIL1fXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPBTWM2OD1yLkC2NlU2KM7:TR?= M1HlWnNCVkeHUh?=
G-402 NY\ZUndjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmD4TWM2OD1yLkC2OFYh|ryP MV3TRW5ITVJ?
ES1 NYnnSpRGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDETWM2OD1yLkC3NVQ3KM7:TR?= MYnTRW5ITVJ?
L-363 NWrqPIo1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHG2eY5KSzVyPUCuNFc{QSEQvF2= NX7tWGRsW0GQR1XS
A4-Fuk MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTBwMEe0PFYh|ryP NED5bFlUSU6JRWK=
OC-314 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTBwMEe4NFch|ryP MknSV2FPT0WU
SK-LU-1 MnvoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPDeoRKSzVyPUCuNFg1OjFizszN M3H2c3NCVkeHUh?=
MDA-MB-157 MmTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfLeHNKSzVyPUCuNFg3PTlizszN NX60XGdRW0GQR1XS
HuP-T3 NE\PcXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPweFFEUUN3ME2wMlA5PzFizszN NGDDfW5USU6JRWK=
A3-KAW NYfWPJFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInlTZFKSzVyPUCuNFg5ODZizszN NH\pXnFUSU6JRWK=
MOLT-16 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7KemRKSzVyPUCuNFg5ODdizszN M4\sfnNCVkeHUh?=
COLO-679 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkW3TWM2OD1yLkC5NFkyKM7:TR?= NUXZdVdEW0GQR1XS
EM-2 NUPDO49UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTBwMEm5NFMh|ryP MUnTRW5ITVJ?
ES8 NV;XeI9TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnznTWM2OD1yLkC5PVE5KM7:TR?= M2rYS3NCVkeHUh?=
SK-MEL-2 NX7j[5lIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\TTWM2OD1yLkGwNFM{KM7:TR?= NH[4XolUSU6JRWK=
ES3 NYfaNIVkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Htd2lEPTB;MD6xNFIh|ryP M2DsWnNCVkeHUh?=
HSC-4 MoDqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vWVmlEPTB;MD6xNFU2PCEQvF2= NUj5fGFtW0GQR1XS
697 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTDTWM2OD1yLkGxNFY{KM7:TR?= MX\TRW5ITVJ?
TGBC11TKB MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwMUGwPVMh|ryP MmLaV2FPT0WU
SK-PN-DW MkK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3WTWM2OD1yLkGxPFYh|ryP M{HpcHNCVkeHUh?=
H9 MkPrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\GTWM2OD1yLkGyNVY2KM7:TR?= NGrmVI5USU6JRWK=
NCI-H2122 NIrEdIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPXTWM2OD1yLkGyNlUh|ryP M4HE[3NCVkeHUh?=
ML-2 NG\HZphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTBwMUK0PFQh|ryP MlLsV2FPT0WU
MC-IXC MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXKyVG4xUUN3ME2wMlEzQDR|IN88US=> MnLBV2FPT0WU
KYSE-140 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XRc2lEPTB;MD6xN|A1OSEQvF2= M1Pn[nNCVkeHUh?=
DOHH-2 MoPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXz1XpE3UUN3ME2wMlE{ODZ2IN88US=> NVXoTlJyW0GQR1XS
MEL-JUSO MnnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nCU2lEPTB;MD6xN|EzQCEQvF2= NFuy[VRUSU6JRWK=
P30-OHK MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTHTWM2OD1yLkGzNVYh|ryP M3G0THNCVkeHUh?=
CTB-1 NFnrWI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTBwMUOzNlUh|ryP M4Tx[HNCVkeHUh?=
ACHN NVzOO4RUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTBwMUOzOlkh|ryP MW\TRW5ITVJ?
ES5 MoLVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTBwMUO0O|Yh|ryP NFPje3pUSU6JRWK=
SF539 MnzWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjxemFzUUN3ME2wMlE{PjhizszN Mn\pV2FPT0WU
D-283MED NFjGe4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwMUO2PFch|ryP MWjTRW5ITVJ?
OVCAR-5 NYHWUFZ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvZTWM2OD1yLkGzPFk1KM7:TR?= M{XkPHNCVkeHUh?=
RPMI-8866 NU[0SoRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonkTWM2OD1yLkGzPVI2KM7:TR?= M3XLW3NCVkeHUh?=
PANC-03-27 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnX0TWM2OD1yLkG0Nlg5KM7:TR?= NYLTfFc5W0GQR1XS
M14 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmSwTWM2OD1yLkG0OFY{KM7:TR?= NX3VcldYW0GQR1XS
D-566MG Ml[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jOeWlEPTB;MD6xOFY6KM7:TR?= Mn7NV2FPT0WU
Ramos-2G6-4C10 NYDpW2RnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwMUS4OFIh|ryP NVvseGdZW0GQR1XS
NOS-1 M3v2ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTBwMUS5Olch|ryP NIfidmZUSU6JRWK=
YH-13 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnETIhKSzVyPUCuNVU3PzRizszN MUHTRW5ITVJ?
NB17 NWTNZ3JTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPyUGFCUUN3ME2wMlE2Pzh4IN88US=> NWj1T4R{W0GQR1XS
U-2-OS NWrQ[IhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HUTGlEPTB;MD6xOVg1PyEQvF2= MYXTRW5ITVJ?
ES7 M4fCVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnBbFBKSzVyPUCuNVYxOTFizszN NH7mR5RUSU6JRWK=
LoVo M4LuVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLyOWFLUUN3ME2wMlE3OTZ4IN88US=> NHzMUo9USU6JRWK=
QIMR-WIL NULXOWFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4OzVmlEPTB;MD6xOlMyOSEQvF2= NGfC[3FUSU6JRWK=
ESS-1 MonVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LrT2lEPTB;MD6xOlQyOyEQvF2= NHrGeGFUSU6JRWK=
LAMA-84 NVTSfmNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPROIhsUUN3ME2wMlE3PTB|IN88US=> M{\4NHNCVkeHUh?=
TGBC24TKB MkjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\vTWM2OD1yLkG2OVM2KM7:TR?= NWHRcnlRW0GQR1XS
KU812 Mli2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{eyWGlEPTB;MD6xOlg{KM7:TR?= M1KwRXNCVkeHUh?=
KARPAS-45 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DLfGlEPTB;MD6xOlg1PyEQvF2= M2T0PXNCVkeHUh?=
ACN NFrXV|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1q5TmlEPTB;MD6xO|E4OSEQvF2= MkXhV2FPT0WU
RPMI-8226 NEnyeFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTBwMUe0OlQh|ryP Mn3DV2FPT0WU
GR-ST MnO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LRS2lEPTB;MD6xO|gzPyEQvF2= MVvTRW5ITVJ?
SK-UT-1 MnfwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWr1eWxDUUN3ME2wMlE5PTl7IN88US=> MlXsV2FPT0WU
SK-N-AS MlTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13iO2lEPTB;MD6xPFgyPSEQvF2= NV;YdWpCW0GQR1XS
EW-1 MoDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljvTWM2OD1yLkG4PVA2KM7:TR?= MWPTRW5ITVJ?
5637 NYTK[FIxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nl[2lEPTB;MD6xPVI4QCEQvF2= NFPye|lUSU6JRWK=
GI-1 M{LXOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TvPWlEPTB;MD6xPVU3OSEQvF2= Mm\BV2FPT0WU
NCI-H1395 NFrXSFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPTcpBuUUN3ME2wMlE6PzZzIN88US=> NGrXZ2pUSU6JRWK=
CHP-212 NFHSVWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLNdYNkUUN3ME2wMlE6Pzh{IN88US=> M1Txe3NCVkeHUh?=
A427 M3PBTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;KO21KSzVyPUCuNVk5OzJizszN MUnTRW5ITVJ?
BE-13 NUO2fHQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPlbIpnUUN3ME2wMlIxOTF5IN88US=> NXvacW5bW0GQR1XS
RS4-11 MkD0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\4RllKSzVyPUCuNlA{OThizszN MWrTRW5ITVJ?
TI-73 NU[2NW9uT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DPWmlEPTB;MD6yNFg4OyEQvF2= NGDmepVUSU6JRWK=
HAL-01 M{jz[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvsTWM2OD1yLkKwPVch|ryP M2HPV3NCVkeHUh?=
KY821 M3z3bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX74[phXUUN3ME2wMlIyPDJ7IN88US=> NX31OVduW0GQR1XS
H4 M1XsWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXPTWM2OD1yLkKyNFYzKM7:TR?= MXXTRW5ITVJ?
EPLC-272H M{XocWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NECwXXBKSzVyPUCuNlIxPjhizszN MWTTRW5ITVJ?
COR-L105 M2DwdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzvcHJKSzVyPUCuNlIzOzVizszN Mo\uV2FPT0WU
769-P MnniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkn1TWM2OD1yLkKyNlk5KM7:TR?= NVfvOoZnW0GQR1XS
HEL MoHKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXNPFlKSzVyPUCuNlI1PTRizszN M4\CZ3NCVkeHUh?=
VA-ES-BJ NX;TO3ZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTBwMkK1OVch|ryP Mn3PV2FPT0WU
IST-MEL1 NFTCXHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvITWM2OD1yLkKyOVch|ryP MWrTRW5ITVJ?
KYSE-270 M1LL[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\hTWM2OD1yLkKyOlM{KM7:TR?= MoDxV2FPT0WU
RH-1 M4jPNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwMkK2OlYh|ryP MX\TRW5ITVJ?
JVM-3 NUfh[XBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTBwMkOwN|Yh|ryP MVPTRW5ITVJ?
RPMI-7951 M2G0SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTBwMkOwOVYh|ryP MYDTRW5ITVJ?
JAR M3naeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\ISWlEPTB;MD6yN|I{QCEQvF2= NFrnT4pUSU6JRWK=
MOLT-4 NEn6O21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTBwMkO4OUDPxE1? NF;xd2RUSU6JRWK=
ALL-PO M37hb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTBwMkSyOFMh|ryP MoDJV2FPT0WU
SNU-387 M2nkcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjSNXFKSzVyPUCuNlQ{OzlizszN MVXTRW5ITVJ?
IGROV-1 NIm0Z4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XtRWlEPTB;MD6yOFQxOSEQvF2= M2P4c3NCVkeHUh?=
MPP-89 NF\C[mdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHaTWM2OD1yLkK0OFUyKM7:TR?= M3fMVXNCVkeHUh?=
T98G NUPyeHZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUiy[GVRUUN3ME2wMlI1PTZ6IN88US=> NFrPdXBUSU6JRWK=
NCI-H510A M3rXVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13ZTmlEPTB;MD6yOFYzPiEQvF2= NGnO[GpUSU6JRWK=
KOSC-2 MnqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXJemlKSzVyPUCuNlQ3PzFizszN NXTRRXBIW0GQR1XS
NCI-H23 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMkS3OlEh|ryP NYfYZmpnW0GQR1XS
MC116 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHu1S3VKSzVyPUCuNlU3PTRizszN M1nTZXNCVkeHUh?=
8-MG-BA M1TjSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLkTWM2OD1yLkK1O|Y5KM7:TR?= NV76[VE6W0GQR1XS
ABC-1 MmW1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXNTWM2OD1yLkK2NFY1KM7:TR?= MmroV2FPT0WU
J-RT3-T3-5 Mo[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;IPGxKSzVyPUCuNlY3PzhizszN MYrTRW5ITVJ?
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BB65-RCC NYHTflR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPM[nh6UUN3ME2wMlI4ODl5IN88US=> NInLO3NUSU6JRWK=
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OVCAR-3 MnG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfZWoRKSzVyPUCuNlc{QDVizszN M3fPVXNCVkeHUh?=
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MSTO-211H MoftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPvbWhXUUN3ME2wMlI6QDV3IN88US=> M1TVXnNCVkeHUh?=
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HCC70 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTBwM{C2OlUh|ryP MUDTRW5ITVJ?
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LCLC-97TM1 NWHBWoFIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfVOHd1UUN3ME2wMlMyPTZ4IN88US=> NGK5d3ZUSU6JRWK=
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OVCAR-4 MkTUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGrpWWZKSzVyPUCuN|IxOjFizszN M{\Ke3NCVkeHUh?=
NCI-H209 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjITWM2OD1yLkOyN|U{KM7:TR?= MofIV2FPT0WU
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KALS-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfXTWM2OD1yLkOyOVk1KM7:TR?= MoDiV2FPT0WU
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Detroit562 NGXtbWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfUTWM2OD1yLkOzN|Q{KM7:TR?= NFH6UZRUSU6JRWK=
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MEG-01 Mnv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfGV4xKSzVyPUCuN|Q2QDRizszN NVuzVmZZW0GQR1XS
BV-173 MlOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVy3dIQ2UUN3ME2wMlM1PTl4IN88US=> MY\TRW5ITVJ?
MZ7-mel M2S4b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH:2RlVKSzVyPUCuN|Q3PzdizszN M3zI[3NCVkeHUh?=
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KP-N-YN NGjQSI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPWNFVTUUN3ME2wMlM1QDh5IN88US=> MWPTRW5ITVJ?
MKN1 NH;KdnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTnNHVrUUN3ME2wMlM2ODB{IN88US=> NGnOb|NUSU6JRWK=
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OVCAR-8 Ml3OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTBwM{W1PFQh|ryP NYnBWFljW0GQR1XS
NH-12 MkjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoC2TWM2OD1yLkO1Olk3KM7:TR?= NGfBW3JUSU6JRWK=
COR-L88 NWDqNm9ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1f1O2lEPTB;MD6zOlQ5OiEQvF2= NVn0R3VpW0GQR1XS
T-24 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHoNnBKSzVyPUCuN|Y6QDVizszN MVPTRW5ITVJ?
MIA-PaCa-2 M{O3PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULWRY5xUUN3ME2wMlM4PDl5IN88US=> MYjTRW5ITVJ?
LS-411N M{PvTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Moj1TWM2OD1yLkO3OlA3KM7:TR?= Mm\6V2FPT0WU
TK10 MontS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTBwM{e5OFgh|ryP M13CfXNCVkeHUh?=
SW1990 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrP[XpUUUN3ME2wMlM5OzV|IN88US=> MmTmV2FPT0WU
GAMG NGD0[4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHqTWM2OD1yLkO4OVY4KM7:TR?= NFL0OFZUSU6JRWK=
G-361 M{HsRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37zNmlEPTB;MD6zPFcyOiEQvF2= MVzTRW5ITVJ?
Ca-Ski M4jxNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zCXGlEPTB;MD6zPFc6OiEQvF2= NX7FN|FkW0GQR1XS
786-0 NHjmWFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLNW|FKSzVyPUCuN|g5OjNizszN MW\TRW5ITVJ?
MHH-NB-11 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzmfG5KSzVyPUCuN|g6PyEQvF2= MoTKV2FPT0WU
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SW954 M1LCVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1mybWlEPTB;MD60NFE{OSEQvF2= M1HqT3NCVkeHUh?=
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NCI-H720 NXzXR2lTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{TlU2lEPTB;MD60NFE6OSEQvF2= MoX0V2FPT0WU
COR-L23 NVHTPWtTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml6xTWM2OD1yLkSwNlg3KM7:TR?= MUXTRW5ITVJ?
PFSK-1 M{HZ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvTTWM2OD1yLkSxNFkzKM7:TR?= M3;KVHNCVkeHUh?=
FADU MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXOzfVZKUUN3ME2wMlQyOTd{IN88US=> NHjYdYZUSU6JRWK=
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PC-14 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPGOVhXUUN3ME2wMlQyPjZzIN88US=> NVjVWI1IW0GQR1XS
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D-336MG M13zXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1r5bWlEPTB;MD60NlE{QCEQvF2= MV7TRW5ITVJ?
LOXIMVI M4ryVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnFTWM2OD1yLkSyPFg2KM7:TR?= NFO4c45USU6JRWK=
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A2780 NXj5d3FQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPjO4xKSzVyPUCuOFMyPjJizszN MVLTRW5ITVJ?
CAL-12T M1juSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTBTWM2OD1yLkSzNlg5KM7:TR?= NFLMfI1USU6JRWK=
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A2058 NFPiU|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M165d2lEPTB;MD60OVE{PiEQvF2= MYDTRW5ITVJ?
Hs-578-T MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDWWW5KSzVyPUCuOFUzPzhizszN M33UTHNCVkeHUh?=
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D-392MG NVjVfmdST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGjaTG5KSzVyPUCuOFU3OTJizszN MVnTRW5ITVJ?
MOLT-13 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTBwNE[yPVQh|ryP NX7vdoFUW0GQR1XS
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NCI-H1563 NGX6WolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDKUWhKSzVyPUCuOFcxOzVizszN M3\hO3NCVkeHUh?=
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HT-3 NIPodZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwNEmwN{DPxE1? NEL2e5pUSU6JRWK=
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CAL-51 M1zndGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT6eW5{UUN3ME2wMlUyPDN5IN88US=> MVzTRW5ITVJ?
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CHL-1 MlPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;Q[3JsUUN3ME2wMlUzPTVzIN88US=> MVXTRW5ITVJ?
SK-N-DZ MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzKdJdDUUN3ME2wMlU{ODd6IN88US=> NVfkUJRQW0GQR1XS
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NB13 M4Xuemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;icZdTUUN3ME2wMlU{PjV5IN88US=> NXr3S5o6W0GQR1XS
EW-16 MlXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\FOotNUUN3ME2wMlU{Pzd2IN88US=> NYHaVVRWW0GQR1XS
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CAL-62 NW\oW3N[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnm5TWM2OD1yLkW3NVIyKM7:TR?= NYjkUmw1W0GQR1XS
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EW-22 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIK3O4ZKSzVyPUCuOVg{PzVizszN NHL5ZnlUSU6JRWK=
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BFTC-909 NEfQZmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HpTmlEPTB;MD61PFg{OyEQvF2= MUfTRW5ITVJ?
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NB10 M13kRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\XSGlEPTB;MD61PVEzQSEQvF2= MVHTRW5ITVJ?
COLO-800 M4TxZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfsS25zUUN3ME2wMlYxOzJ6IN88US=> M4nsRnNCVkeHUh?=
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VM-CUB-1 M4TsW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTJ[Ig3UUN3ME2wMlYyQDZzIN88US=> Ml7ZV2FPT0WU
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NCI-H226 MmHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoH1TWM2OD12LkW0PFcyKM7:TR?= MVHTRW5ITVJ?
CAL-27 M2LncGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3eySmlEPTB;ND63NVY6PCEQvF2= MUXTRW5ITVJ?
G-401 NEPTSXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTRwOEO4OFEh|ryP M4\5bnNCVkeHUh?=
CAL-120 M4fJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTRwOES5PVEh|ryP MlnwV2FPT0WU
DMS-114 Mme3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rxWWlEPTB;NT6xOFUxPiEQvF2= NHHDOnZUSU6JRWK=
KYSE-70 NEnvT5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3G0SWlEPTB;NT6xOFg2QSEQvF2= NFzx[GFUSU6JRWK=
NCI-H1648 NFHPbXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnqxTWM2OD13LkK5O|gzKM7:TR?= NFjvb|dUSU6JRWK=
MDA-MB-175-VII NYPZUVB3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2OwSWlEPTB;NT60OFAyPCEQvF2= MUTTRW5ITVJ?
NCI-H2029 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWWyXnQzUUN3ME21MlQ5ODJ{IN88US=> MkC1V2FPT0WU
LS-1034 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTVwNk[4PFkh|ryP M4L3PXNCVkeHUh?=
LB831-BLC MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPWTWM2OD13LkewN|Q2KM7:TR?= NX\3fJJsW0GQR1XS
MDA-MB-415 M4Xte2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVS0XmFiUUN3ME21MlcxQDFizszN NX7UZZhvW0GQR1XS
TE-6 NHLuXm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDjZW5KSzVyPUWuO|QyQDJizszN NXHuVoUyW0GQR1XS
SK-MEL-24 MmT2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLkT4ZtUUN3ME21Mlc1PTZ2IN88US=> MXjTRW5ITVJ?
HUTU-80 MoDDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\teIlKSzVyPUWuO|U{OzNizszN NFLPU3ZUSU6JRWK=
OCUB-M Mk[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1r3VGlEPTB;NT63O|U3OyEQvF2= MYPTRW5ITVJ?
IGR-1 Mn;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTvXYpuUUN3ME21Mlg3QDl2IN88US=> NFzCR4tUSU6JRWK=
NCI-H747 NYfablVMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HhdWlEPTB;Nj6yOFU5PSEQvF2= MV3TRW5ITVJ?
COLO-680N NFf6fFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7JUVJKSzVyPU[uN|k1ODNizszN NIf0TWNUSU6JRWK=
NB12 NGLBU4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{f4R2lEPTB;Nj63PVczOiEQvF2= M3fPfHNCVkeHUh?=
SNU-449 NFrwU3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH70VZRKSzVyPU[uPVk2QTFizszN MY\TRW5ITVJ?
D-542MG M2LLPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\HTWM2OD15LkGyNlk6KM7:TR?= MmHNV2FPT0WU
COLO-792 NVrwe3V7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTdwOEexNlch|ryP MnfXV2FPT0WU
NCI-H810 NV7oRlVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;XVmk2UUN3ME24MlAzQTh2IN88US=> MlLLV2FPT0WU
T47D MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XVO2lEPTB;OD6xOlAzPCEQvF2= Mk[zV2FPT0WU
Calu-3 MmDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRThwMki4PFYh|ryP MkK5V2FPT0WU
COLO-741 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRThwOUOwPVMh|ryP M3e0NXNCVkeHUh?=
LU-135 Mo[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnudJRKSzVyPUmuNFM1PTJizszN NFzXdmtUSU6JRWK=
COLO-678 MnPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnFTWM2OD17LkK4PFQ1KM7:TR?= Mk\mV2FPT0WU
NCI-H2126 NYfJWHNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2G4ZmlEPTB;OT6zN|kyQSEQvF2= M{nDfHNCVkeHUh?=
SK-MEL-28 Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2qyOmlEPTB;OT61N|U6PiEQvF2= MVTTRW5ITVJ?
MFE-280 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLVTWM2OD1zMD6wNlI6KM7:TR?= MVfTRW5ITVJ?
DBTRG-05MG NH;ufHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrEUHlpUUN3ME2xNE41OzZ7IN88US=> NEn4fnFUSU6JRWK=
RCC10RGB M4jQVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfjVlAxUUN3ME2xNE42OjV3IN88US=> NFWwVWhUSU6JRWK=
NTERA-S-cl-D1 NH\lZVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zmSGlEPTB;MUCuOlg2OiEQvF2= NGPyb4JUSU6JRWK=
C3A MlPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTF{LkOxO|Ih|ryP MYHTRW5ITVJ?
MHH-ES-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTF{LkWyPFQh|ryP MmnxV2FPT0WU
LU-139 NVrzc5dtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTF{Lk[zN|Mh|ryP MVHTRW5ITVJ?
KNS-42 NEnw[pVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTF{Lki3O|Eh|ryP MWXTRW5ITVJ?
MCF7 NYPTO3k6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;GTWM2OD1zMz6yN|Y2KM7:TR?= NIHEVHFUSU6JRWK=
SW872 NGLTRmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPJTWM2OD1zND61OFYyKM7:TR?= MVfTRW5ITVJ?
HT-1376 M3r1e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M361NGlEPTB;MUSuPFU5OiEQvF2= M36xVXNCVkeHUh?=
NCI-SNU-5 NIHpUVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Lrc2lEPTB;MUWuNVc1OSEQvF2= NUPVd3dUW0GQR1XS
NCI-H2405 NUL3eFh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFOwXXJKSzVyPUG1MlQ1PzRizszN MkTvV2FPT0WU
KYSE-150 NWfBS3NiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlH2TWM2OD1zNT63OlEyKM7:TR?= MWXTRW5ITVJ?
SW837 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoniTWM2OD1zNj6wNVI1KM7:TR?= MnOyV2FPT0WU
LU-65 M1TUNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zFOmlEPTB;MUmuO|I3KM7:TR?= MWLTRW5ITVJ?
JEG-3 NYfwSFBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzzTWM2OD1{MD6zOVc4KM7:TR?= NXfrTVFwW0GQR1XS
SK-MEL-1 M3vJSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TH[GlEPTB;MkCuO|g{PiEQvF2= NID3WFBUSU6JRWK=
SK-HEP-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nNNGlEPTB;MkGuOVU1OSEQvF2= MUjTRW5ITVJ?
NCI-H1770 NYT5PW9kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTJ|LkSwPFYh|ryP Mn\QV2FPT0WU
MG-63 M2rPSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPaTWM2OD1{Mz63Olc{KM7:TR?= NIr2d|dUSU6JRWK=
ECC10 M3zyVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nHd2lEPTB;MkWuOVcyKM7:TR?= MUXTRW5ITVJ?
SW1463 NF\lUWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPKSY5OUUN3ME2yO{45OzF4IN88US=> NFTLc49USU6JRWK=
JVM-2 MlrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvMZXRKSzVyPUK4MlMzPDhizszN NFnVfHpUSU6JRWK=
HCT-15 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zVSmlEPTB;MkiuOFE6OiEQvF2= MUHTRW5ITVJ?
CAL-72 NH3oXnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHe3cpdKSzVyPUOxMlQ6OTJizszN MX;TRW5ITVJ?
HCC2218 M4PDUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTN{Lki4OVgh|ryP NITLeVZUSU6JRWK=
BT-474 NGfENHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7yTWM2OD1|Mz6wPFQ{KM7:TR?= M1LOXHNCVkeHUh?=
NCI-H1092 NF\qRZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXqTWM2OD1|Mz61NVQ2KM7:TR?= NVjzbHNFW0GQR1XS
Mo-T NGL4RXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M375SmlEPTB;M{euN|U5PSEQvF2= Mn3EV2FPT0WU
DU-145 NHrHOpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTRyLk[yPVUh|ryP MXHTRW5ITVJ?
NCI-H1666 NH\RfpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13ONmlEPTB;NEKuOFQ5QCEQvF2= MX3TRW5ITVJ?
IST-MES1 NGThfZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDtUXdKSzVyPUS2MlQyQTZizszN MU\TRW5ITVJ?
SW1116 NHXWXFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYW3cpI3UUN3ME20PE4yPzV4IN88US=> NUHld45JW0GQR1XS
MZ2-MEL MlrBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlntTWM2OD12OD61NFI3KM7:TR?= MlrXV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pChk1 / Chk1 / Wee1 / p21 / p53 ; 

PubMed: 29963769     


Immunoblotting analysis of CAL39 and SW954 cells showing increase in CHK1 phosphorylation after exposure to AZD7762 for 24 hours. β‐actin is used as loading control. Representative data from n = 3 experiments.

29963769
Growth inhibition assay
Cell viability; 

PubMed: 23715154     


Lkb1-wt (634, 855, and 857) and Lkb1-null (t2, t4, and t5) cell lines were plated into 96-well plates at 2000 cells/well in 150 ml medium containing the indicated concentrations of AZD7762 or CHIR124 for 3 days. Viable cells were then counted with Dojindo’s Cell Counting Kit-8 assay. The data represent mean ± SD for 3 repeats. GI50 was calculated with GraphPad.

23715154
In vivo AZD7762 alone at 25 mg/kg shows little antitumor activity in the H460-DNp53 xenograft mice and SW620 xenograft mice, but when administrated in combination with gemcitabine (60 mg/kg), AZD7762 shows significant antitumor efficacy in the two xenografts mice with a log cell kill of 0.9 or percent treated/control (%T/C) of 26 even at low dose of 12.5 mg. Dosing of AZD7762 in combination with gemcitabine (10 mg/kg) in the H460-DNp53 xenograft rat inhibits the tumor volume in a dose-dependent manner with the %T/C values of 48 and 32 for 10 and 20 mg/kg AZD7762, respectively. AZD7762 (25 mg/kg) in combination with irinotecan (25 or 50 mg/kg) causes complete tumor regression in the SW620 xenograft mice with the %T/C increasing significantly to -66% and -67%, respectively. [1]

Protocol

Kinase Assay:[1]
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Chk1 Kinase Assay:

Recombinant human Chk1 is expressed as a glutathione S-transferase fusion in insect cells using a baculovirus vector and purified by glutathione affinity chromatography. A synthetic peptide substrate (N-biotinylaminohexanoyl-KKVSRSGLYRSPMPENLNRPR) for Chk1 is synthesized. Final assay concentrations of peptide and ATP (cold + 40 nCi [33P]ATP) are 0.8 and 1 μM, respectively. Different concentrations of AZD7762, buffer containing peptide and chk1 kinase and ATP, are added sequentially to a 384-well assay plate. The plate is incubated for 2 hours, reaction is stopped by the addition of buffer containing EDTA and scintillation proximity assay beads, and plates are read using a TopCount reader. Data analysis is carried out to determinate a dose response (IC50).
Cell Research:[1]
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  • Cell lines: HT29, SW620 and MDA-MB-231 cells
  • Concentrations: Dissolved in DMSO, final concentration ~12.5 μM
  • Incubation Time: 20 or 48 hours
  • Method: For the checkpoint abrogation assay, HT29 cells are treated for 2 hours with camptothecin (topoisomerase I inhibitor; 0.07 μg/mL) to induce the G2 checkpoint. Cells are then treated for 20 hours with a 12-point titration of AZD7762 (12.5 μM to 6 nM) plus nocodazole. Cells are fixed with 3.7% formaldehyde for 1 hour, permeabilized with PBS containing 0.05% Triton X, and incubated with anti-phH3 antibody for 1 hour followed by Alexa Fluor 488 anti-rabbit and Hoechst stain for 1 hour. Mitotic index is determined on the ArrayScan and expressed as the percentage of cells undergoing mitosis. For the potentiation assays, SW620 or MDA-MB-231 cells are dosed for 24 hours with a 9-point titration of gemcitabine ranging from 0.01 to 100 nM with a constant dose of AZD7762 (300 nM). After 24 hours, medium is removed and AZD7762 alone is added back to the wells for an additional 24 hours. Cells are then incubated in AZD7762-free medium for an additional 72 hours. The effect on cell proliferation is determined by MTT.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Male NCr mice implanted s.c. with H460-DNp53 cells or SW620, and male rnu rats implanted s.c. with H460-DNp53 cells
  • Dosages: ~25 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (137.96 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.42
Formula

C17H19FN4O2S

CAS No. 860352-01-8
Storage powder
in solvent
Synonyms N/A
Smiles C1CC(CNC1)NC(=O)C2=C(C=C(S2)C3=CC(=CC=C3)F)NC(=O)N

In vivo Formulation Calculator (Clear solution)

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    If I want to use the inhibitor for i.p. injection, can I use DMSO to dissolve it?

  • Answer:

    Since DMSO has low toxicity with i.p. injection, hydroxyproplyl-β-cyclodextrin is consider a safer co-solvent in compound formulation. In a previous literature report, 11.3% hydroxyproplyl-β-cyclodextrin was used to formulate this compound for i.v. injection.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID