AZD7762

Catalog No.S1532

AZD7762 Chemical Structure

Molecular Weight(MW): 362.42

AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.

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In DMSO USD 192 In stock
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Cited by 55 Publications

Purity & Quality Control

Choose Selective Chk Inhibitors

Biological Activity

Description AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.
Targets
Chk1 [1]
(Cell-free assay)
Chk2 [1]
(Cell-free assay)
5 nM <10 nM
In vitro

AZD7762, a more selective Chk1 inhibitor, inhibits Chk1 phosphorylation of a cdc25C peptide by reversibly binding to the ATP-binding site of Chk1, with IC50 of 5 nM and Ki of 3.6 nM. AZD7762 induces cell arrest with EC50 of 0.620 μM, and significantly abrogates the camptothecin induced G2 arrest with an EC50 of 10 nM, by blocking the chk1 dependent degradation of Cdc25A and activation of Cyclin A. AZD7762 (300 nM) enhances the antitumor efficacy of gemcitabine against SW620 and topotecan against MDA-MB-231 by reducing the GI50 values from 24.1 nM and 2.25 μM to 1.08 nM and 0.15 μM, respectively. [1] AZD7762 shows cytotoxicity against a variety of neuroblastoma cell lines bearing p53 wild type, p53 mutation, Mdm2 amplification or p14 deletion with IC50 values ranging from 82.6-505.9 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KASUMI-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfaT4JVUUN3ME2wMlAxODJ|NDFOwG0> NH\1eGZUSU6JRWK=
NKM-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M360NmlEPTB;MD6wNFA1OTVizszN MX7TRW5ITVJ?
MV-4-11 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTBwMECwOFI2KM7:TR?= NEnvUWRUSU6JRWK=
NCI-H1703 NXzOSVBVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTBwMEC2OFMh|ryP NU[0SZFvW0GQR1XS
A204 MnjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLoVnZoUUN3ME2wMlAxQDV4IN88US=> NETRVHVUSU6JRWK=
CGTH-W-1 NVXrfYlyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwMEG0NkDPxE1? M4[5[XNCVkeHUh?=
MLMA MmroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTBwMEK4OFkh|ryP M4HnZXNCVkeHUh?=
GOTO MlTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfkUXVKSzVyPUCuNFMyOiEQvF2= NEPvUIFUSU6JRWK=
KE-37 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\hOJhKSzVyPUCuNFMyPDJizszN MkXXV2FPT0WU
ES4 Mnj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3KTWM2OD1yLkCzNlUyKM7:TR?= MUTTRW5ITVJ?
ATN-1 NG\1WWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHmwNZhKSzVyPUCuNFM1OTdizszN M3[4bXNCVkeHUh?=
MHH-PREB-1 MkC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[w[ZkzUUN3ME2wMlA{PDR{IN88US=> MXnTRW5ITVJ?
SW756 M4T4T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTBwMEO1O|kh|ryP MWPTRW5ITVJ?
CTV-1 MlrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH33PIVKSzVyPUCuNFM5QTZizszN NH\DUVJUSU6JRWK=
HCC1806 NHT6Z5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33COWlEPTB;MD6wOFAzOiEQvF2= NG[0PIFUSU6JRWK=
NEC8 NWrUXXd4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1qzN2lEPTB;MD6wOFIxOyEQvF2= NHPxWGNUSU6JRWK=
P12-ICHIKAWA MoDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjKTWM2OD1yLkC0N|E3KM7:TR?= M3zNc3NCVkeHUh?=
DEL MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXXTWM2OD1yLkC0OVkzKM7:TR?= NYHSdnFWW0GQR1XS
KM12 NHTKSoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NImyc3dKSzVyPUCuNFUyPThizszN NGHrUllUSU6JRWK=
LB1047-RCC M3XtXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2r0O2lEPTB;MD6wOlAzPSEQvF2= Ml;TV2FPT0WU
LB2241-RCC MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTBwME[xNFIh|ryP MWLTRW5ITVJ?
NOMO-1 NWLrd3BQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPGOndKSzVyPUCuNFYyOTVizszN MWXTRW5ITVJ?
NB14 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjJO3JKSzVyPUCuNFYyOzZizszN NFjv[4dUSU6JRWK=
ONS-76 MlPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTBwME[xPVYh|ryP M1LrcHNCVkeHUh?=
HD-MY-Z MoHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTBwME[yOVUh|ryP M{fFVnNCVkeHUh?=
G-402 NIPWNXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlj0TWM2OD1yLkC2OFYh|ryP MX;TRW5ITVJ?
ES1 M3S1[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTBwMEexOFYh|ryP NGrF[3dUSU6JRWK=
L-363 NVTFbGNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHNd3d[UUN3ME2wMlA4OzlizszN MXnTRW5ITVJ?
A4-Fuk MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mln4TWM2OD1yLkC3OFg3KM7:TR?= NXrhd|F3W0GQR1XS
OC-314 Mo\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTBwMEe4NFch|ryP NH\mOXdUSU6JRWK=
SK-LU-1 Mnv3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4W2SWlEPTB;MD6wPFQzOSEQvF2= M3S2T3NCVkeHUh?=
MDA-MB-157 NGLXWlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{G5WWlEPTB;MD6wPFY2QSEQvF2= MoO3V2FPT0WU
HuP-T3 MmW1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwMEi3NUDPxE1? NFXudYpUSU6JRWK=
A3-KAW NEDJTopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vEfGlEPTB;MD6wPFgxPiEQvF2= MVTTRW5ITVJ?
MOLT-16 NIG5UGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIr6OoNKSzVyPUCuNFg5ODdizszN M3f1WHNCVkeHUh?=
COLO-679 M4XTTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLwR4FIUUN3ME2wMlA6ODlzIN88US=> MlvSV2FPT0WU
EM-2 NUDzWHFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfBboFiUUN3ME2wMlA6QTB|IN88US=> NFywZ4hUSU6JRWK=
ES8 NYTrZodpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTBwMEm5NVgh|ryP MkmxV2FPT0WU
SK-MEL-2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTBwMUCwN|Mh|ryP MnS0V2FPT0WU
ES3 NUKxdHpKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGjTRmhKSzVyPUCuNVAzKM7:TR?= MUTTRW5ITVJ?
HSC-4 NUDjSo9HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofUTWM2OD1yLkGwOVU1KM7:TR?= M1X5dXNCVkeHUh?=
697 NH7hZ4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXtTWM2OD1yLkGxNFY{KM7:TR?= MnPsV2FPT0WU
TGBC11TKB NYDCNGdjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\DRlR4UUN3ME2wMlEyODl|IN88US=> M1fDcHNCVkeHUh?=
SK-PN-DW Mor6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXHU45KSzVyPUCuNVE5PiEQvF2= M3XqbnNCVkeHUh?=
H9 MoX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzPTWM2OD1yLkGyNVY2KM7:TR?= NXXYV2t3W0GQR1XS
NCI-H2122 Mlq2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrKTWM2OD1yLkGyNlUh|ryP NID4WGpUSU6JRWK=
ML-2 M3PPOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlf0TWM2OD1yLkGyOFg1KM7:TR?= NH\jSWpUSU6JRWK=
MC-IXC MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\IS4FWUUN3ME2wMlEzQDR|IN88US=> Ml\CV2FPT0WU
KYSE-140 M4Gybmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTBwMUOwOFEh|ryP M1viPXNCVkeHUh?=
DOHH-2 NIXJSZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jZTGlEPTB;MD6xN|A3PCEQvF2= MnXWV2FPT0WU
MEL-JUSO M3PX[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTBwMUOxNlgh|ryP MWHTRW5ITVJ?
P30-OHK M3Lr[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjIe5Z5UUN3ME2wMlE{OTZizszN M3qwUHNCVkeHUh?=
CTB-1 Mn35S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkO0TWM2OD1yLkGzN|I2KM7:TR?= NWTTdZVrW0GQR1XS
ACHN M4PvT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnlTow2UUN3ME2wMlE{OzZ7IN88US=> NVHSV2pHW0GQR1XS
ES5 NULyPYE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jkfmlEPTB;MD6xN|Q4PiEQvF2= MVHTRW5ITVJ?
SF539 NUTvTnRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zmO2lEPTB;MD6xN|Y5KM7:TR?= MorIV2FPT0WU
D-283MED NXLRbXRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnG2TWM2OD1yLkGzOlg4KM7:TR?= NIrJXoFUSU6JRWK=
OVCAR-5 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2C1[GlEPTB;MD6xN|g6PCEQvF2= NFfBW|NUSU6JRWK=
RPMI-8866 MnPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInxR4ZKSzVyPUCuNVM6OjVizszN MYLTRW5ITVJ?
PANC-03-27 M3\Bfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofTTWM2OD1yLkG0Nlg5KM7:TR?= NYKyOlhuW0GQR1XS
M14 NF7iNJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3P5bWlEPTB;MD6xOFQ3OyEQvF2= NVS1TphGW0GQR1XS
D-566MG M3;jb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvmNJl[UUN3ME2wMlE1PjlizszN NIfBVIlUSU6JRWK=
Ramos-2G6-4C10 M3vvW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTBwMUS4OFIh|ryP M4rBS3NCVkeHUh?=
NOS-1 NGTQVpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwMUS5Olch|ryP MlLOV2FPT0WU
YH-13 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\jRoRKSzVyPUCuNVU3PzRizszN M1PDXXNCVkeHUh?=
NB17 NEnvXmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfVTWM2OD1yLkG1O|g3KM7:TR?= NH\PXWFUSU6JRWK=
U-2-OS NWHpOZdTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HBUWlEPTB;MD6xOVg1PyEQvF2= Mn\nV2FPT0WU
ES7 NFP1UlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn6V5B{UUN3ME2wMlE3ODFzIN88US=> M4DPR3NCVkeHUh?=
LoVo M4nwUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7sOIRKSzVyPUCuNVYyPjZizszN M1PoNXNCVkeHUh?=
QIMR-WIL NXnlcHhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1y5V2lEPTB;MD6xOlMyOSEQvF2= MWHTRW5ITVJ?
ESS-1 M2LRZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\OU3NmUUN3ME2wMlE3PDF|IN88US=> Mn\TV2FPT0WU
LAMA-84 MoLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\4bmZKSzVyPUCuNVY2ODNizszN M{DteXNCVkeHUh?=
TGBC24TKB NELDfXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHVbVFKSzVyPUCuNVY2OzVizszN Mnz4V2FPT0WU
KU812 NWXuN2ZLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rF[GlEPTB;MD6xOlg{KM7:TR?= NVjqOI01W0GQR1XS
KARPAS-45 NE[zeoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTBwMU[4OFch|ryP MkfyV2FPT0WU
ACN Moq0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV:1UHJRUUN3ME2wMlE4OTdzIN88US=> MmKxV2FPT0WU
RPMI-8226 MmG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHpVItKSzVyPUCuNVc1PjRizszN M4\RdHNCVkeHUh?=
GR-ST MorGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXSUnRSUUN3ME2wMlE4QDJ5IN88US=> NFvGNo9USU6JRWK=
SK-UT-1 MkDlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XEXWlEPTB;MD6xPFU6QSEQvF2= M2TPcXNCVkeHUh?=
SK-N-AS NVr1bFBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoD1TWM2OD1yLkG4PFE2KM7:TR?= M3\vVnNCVkeHUh?=
EW-1 MlW0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXDcJlFUUN3ME2wMlE5QTB3IN88US=> M2TjUnNCVkeHUh?=
5637 NF35ZW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULFVGpbUUN3ME2wMlE6Ojd6IN88US=> NUDRVJpPW0GQR1XS
GI-1 M{jzfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\LZ3BKSzVyPUCuNVk2PjFizszN NFmyUZpUSU6JRWK=
NCI-H1395 NFTJ[GtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYKxW2JSUUN3ME2wMlE6PzZzIN88US=> NUnPcXhLW0GQR1XS
CHP-212 Moe3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vkZWlEPTB;MD6xPVc5OiEQvF2= NXPvdIkyW0GQR1XS
A427 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPWTXh[UUN3ME2wMlE6QDN{IN88US=> NGrE[FJUSU6JRWK=
BE-13 NHXVXmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7nSnozUUN3ME2wMlIxOTF5IN88US=> MX;TRW5ITVJ?
RS4-11 MlfTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwMkCzNVgh|ryP Mn\3V2FPT0WU
TI-73 M{LVfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnr3TWM2OD1yLkKwPFc{KM7:TR?= NFi4d25USU6JRWK=
HAL-01 NITrfYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXlTWM2OD1yLkKwPVch|ryP MX\TRW5ITVJ?
KY821 NXPhcVF4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojsTWM2OD1yLkKxOFI6KM7:TR?= M37CTnNCVkeHUh?=
H4 NUT3O2FJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fXOmlEPTB;MD6yNlA3OiEQvF2= NGXsfllUSU6JRWK=
EPLC-272H M4XkNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrtcYFKSzVyPUCuNlIxPjhizszN NIPj[pdUSU6JRWK=
COR-L105 NEe3VmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTBwMkKyN|Uh|ryP M1ez[XNCVkeHUh?=
769-P M4\PfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF21e5FKSzVyPUCuNlIzQThizszN NFrDSpJUSU6JRWK=
HEL MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfmdnBKSzVyPUCuNlI1PTRizszN M2fsO3NCVkeHUh?=
VA-ES-BJ NVTNUIJMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXJN|BKSzVyPUCuNlI2PTdizszN MUDTRW5ITVJ?
IST-MEL1 Mn;oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nVRWlEPTB;MD6yNlU4KM7:TR?= NX\GPGw2W0GQR1XS
KYSE-270 M{G4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDze3NKSzVyPUCuNlI3OzNizszN NIjWOI5USU6JRWK=
RH-1 NHHS[ItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3kNXFKSzVyPUCuNlI3PjZizszN MnHIV2FPT0WU
JVM-3 MmO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHoSnhKSzVyPUCuNlMxOzZizszN NXnMfmRmW0GQR1XS
RPMI-7951 NIS5fo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTBwMkOwOVYh|ryP Mm[zV2FPT0WU
JAR MnXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTBwMkOyN|gh|ryP MljHV2FPT0WU
MOLT-4 MknOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTBwMkO4OUDPxE1? NI\rV4pUSU6JRWK=
ALL-PO MlTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPVTWM2OD1yLkK0NlQ{KM7:TR?= M4DpUHNCVkeHUh?=
SNU-387 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXW3PJZzUUN3ME2wMlI1OzN7IN88US=> MoHpV2FPT0WU
IGROV-1 NVXL[pMxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMkS0NFEh|ryP NUO2dllOW0GQR1XS
MPP-89 NGjyVJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHSxfI5KSzVyPUCuNlQ1PTFizszN M{PyO3NCVkeHUh?=
T98G MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHeyeGlKSzVyPUCuNlQ2PjhizszN MnrGV2FPT0WU
NCI-H510A NHKzR2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;HSGlEPTB;MD6yOFYzPiEQvF2= M4DmcnNCVkeHUh?=
KOSC-2 MonHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfVTWM2OD1yLkK0OlcyKM7:TR?= NFHwUo5USU6JRWK=
NCI-H23 MkjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\LcGlEPTB;MD6yOFc3OSEQvF2= M3K3fXNCVkeHUh?=
MC116 M{nXXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HzRmlEPTB;MD6yOVY2PCEQvF2= M1HQc3NCVkeHUh?=
8-MG-BA NHm0NIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonwTWM2OD1yLkK1O|Y5KM7:TR?= M2\MWnNCVkeHUh?=
ABC-1 M3;NSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITVNWdKSzVyPUCuNlYxPjRizszN NEXXUpBUSU6JRWK=
J-RT3-T3-5 NWX0RWxNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTBwMk[2O|gh|ryP MWPTRW5ITVJ?
NCI-SNU-1 M3vYWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTBwMkewOVkh|ryP M1iwSXNCVkeHUh?=
HCC1395 MnvCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPIZ3REUUN3ME2wMlI4ODh7IN88US=> NF;hO4pUSU6JRWK=
BB65-RCC NXLrdmZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHK1V5RKSzVyPUCuNlcxQTdizszN MnXEV2FPT0WU
NCI-H1755 M{HFNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwMkezNFQh|ryP NUHqfGwyW0GQR1XS
OVCAR-3 NIjnb5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXWTWM2OD1yLkK3N|g2KM7:TR?= MorTV2FPT0WU
MKN45 MoLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7JdXFKSzVyPUCuNlc6QCEQvF2= M1fyTnNCVkeHUh?=
TYK-nu MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwMkiwOkDPxE1? NUX2eIhbW0GQR1XS
SNU-C2B NInmWHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1L0SWlEPTB;MD6yPFE{PCEQvF2= M37vTnNCVkeHUh?=
H-EMC-SS M4T6TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHGzO3lKSzVyPUCuNlg{PTRizszN NEfrSZBUSU6JRWK=
CAKI-1 NFnkSYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nIbWlEPTB;MD6yPFY5KM7:TR?= M1XOOXNCVkeHUh?=
EW-24 M17QOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTwc3JKSzVyPUCuNlg6OSEQvF2= NUL6ZY5KW0GQR1XS
RXF393 NYj4VWlYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnWZXRKSzVyPUCuNlkxODhizszN NH7ybnNUSU6JRWK=
CCRF-CEM M3G5O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3Sd4JKSzVyPUCuNlkyPTRizszN M3TZdXNCVkeHUh?=
HOP-92 NFzqWVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHznW3FKSzVyPUCuNlkzPzlizszN NHvOUWVUSU6JRWK=
COLO-684 Mle3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHRb4NJUUN3ME2wMlI6PDB4IN88US=> MWLTRW5ITVJ?
NCI-H28 MkDrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPmSJdxUUN3ME2wMlI6PzB2IN88US=> NUC4TG1FW0GQR1XS
HuO9 Mm\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7nTWM2OD1yLkK5O|k{KM7:TR?= MV\TRW5ITVJ?
SF295 NGLW[nRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mly3TWM2OD1yLkK5PFAzKM7:TR?= Mn;CV2FPT0WU
COLO-829 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\0eGI{UUN3ME2wMlI6QDR3IN88US=> M122O3NCVkeHUh?=
MSTO-211H MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\5ZmlEPTB;MD6yPVg2PSEQvF2= MofXV2FPT0WU
MDA-MB-361 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLoTnBZUUN3ME2wMlI6QDl4IN88US=> NGHjd|RUSU6JRWK=
FTC-133 M2i2[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M174S2lEPTB;MD6zNFI3PSEQvF2= MV3TRW5ITVJ?
Mewo NXqxd3g1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIH4N2lKSzVyPUCuN|A1OzFizszN NV\RUo1nW0GQR1XS
HCC70 Ml3US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzCTWM2OD1yLkOwOlY2KM7:TR?= NED6ZWJUSU6JRWK=
BB30-HNC NIXtco1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTBwM{G1NlUh|ryP MVHTRW5ITVJ?
LCLC-97TM1 NEnxT2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnpcHpIUUN3ME2wMlMyPTZ4IN88US=> NYDZXJZWW0GQR1XS
HCC2998 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\K[4lqUUN3ME2wMlMyPjVizszN MWHTRW5ITVJ?
NBsusSR MljUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fybmlEPTB;MD6zNVgyQSEQvF2= M1T4e3NCVkeHUh?=
OVCAR-4 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrpfWVzUUN3ME2wMlMzODJzIN88US=> NIm0dXVUSU6JRWK=
NCI-H209 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonpTWM2OD1yLkOyN|U{KM7:TR?= NGHqd5FUSU6JRWK=
ChaGo-K-1 NXzHNplYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHlO5dKSzVyPUCuN|I1QDlizszN Ml;nV2FPT0WU
KALS-1 NHPp[2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jlV2lEPTB;MD6zNlU6PCEQvF2= MVPTRW5ITVJ?
D-423MG MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXH6N|JsUUN3ME2wMlM{OTR{IN88US=> NW[0W3pbW0GQR1XS
Detroit562 NF7NbY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfoTWM2OD1yLkOzN|Q{KM7:TR?= NWXaOVhQW0GQR1XS
HT-144 Mlf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PlPWlEPTB;MD6zN|gyKM7:TR?= MVLTRW5ITVJ?
MEG-01 MorOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITS[JpKSzVyPUCuN|Q2QDRizszN MVPTRW5ITVJ?
BV-173 NXPYcmJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{SybGlEPTB;MD6zOFU6PiEQvF2= NH3Qe2RUSU6JRWK=
MZ7-mel MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGCwNGFKSzVyPUCuN|Q3PzdizszN MofoV2FPT0WU
C32 M4\3UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3raPGlEPTB;MD6zOFY5OiEQvF2= MWHTRW5ITVJ?
DU-4475 MnTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;ENWlEPTB;MD6zOFg4PSEQvF2= NIfpZVlUSU6JRWK=
KP-N-YN M{fmO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjUT3JxUUN3ME2wMlM1QDh5IN88US=> M2LSTHNCVkeHUh?=
MKN1 NUnBOGRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDFTWM2OD1yLkO1NFAzKM7:TR?= M3y2U3NCVkeHUh?=
8505C MmDTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmP6TWM2OD1yLkO1NFAzKM7:TR?= MkHBV2FPT0WU
OVCAR-8 MkDKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDTeWhKSzVyPUCuN|U2QDRizszN MVPTRW5ITVJ?
NH-12 NYfIcZJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkD4TWM2OD1yLkO1Olk3KM7:TR?= MWHTRW5ITVJ?
COR-L88 NIrvc2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTBwM{[0PFIh|ryP Mnm1V2FPT0WU
T-24 NV3RdVJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwM{[5PFUh|ryP M1K2N3NCVkeHUh?=
MIA-PaCa-2 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\iTWM2OD1yLkO3OFk4KM7:TR?= NIXKXJFUSU6JRWK=
LS-411N M3TZ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLFV2ZKSzVyPUCuN|c3ODZizszN NXjjW|BVW0GQR1XS
TK10 NFHmc|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrxTWM2OD1yLkO3PVQ5KM7:TR?= Ml;tV2FPT0WU
SW1990 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkO1TWM2OD1yLkO4N|U{KM7:TR?= MkTmV2FPT0WU
GAMG NIf4WpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTBwM{i1Olch|ryP MY\TRW5ITVJ?
G-361 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrtTWM2OD1yLkO4O|EzKM7:TR?= M16yTHNCVkeHUh?=
Ca-Ski MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX33Z3FtUUN3ME2wMlM5Pzl{IN88US=> MkfzV2FPT0WU
786-0 NIDY[2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwM{i4NlMh|ryP M3LGZXNCVkeHUh?=
MHH-NB-11 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33ESGlEPTB;MD6zPFk4KM7:TR?= NYHBS25JW0GQR1XS
OE33 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;yRpR[UUN3ME2wMlM6OTF6IN88US=> NWrBZ4x6W0GQR1XS
SW954 MnLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULXVVI5UUN3ME2wMlQxOTNzIN88US=> MYnTRW5ITVJ?
KYSE-180 NF62TnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTBwNECxOVEh|ryP NUW5SJVoW0GQR1XS
NCI-H720 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrhSWM6UUN3ME2wMlQxOTlzIN88US=> M2D6XXNCVkeHUh?=
COR-L23 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTBwNECyPFYh|ryP M2n4R3NCVkeHUh?=
PFSK-1 NFrtWIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TtdWlEPTB;MD60NVA6OiEQvF2= NI\iRVVUSU6JRWK=
FADU MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXBRWpKSzVyPUCuOFEyPzJizszN NX\OXo04W0GQR1XS
GP5d MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;0NXpJUUN3ME2wMlQyOjJ3IN88US=> M1j1[nNCVkeHUh?=
PC-14 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\4TWM2OD1yLkSxOlYyKM7:TR?= NGPwWY5USU6JRWK=
SJSA-1 M{jGb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTBwNEG3NVQh|ryP NYC5PIF1W0GQR1XS
D-336MG Ml\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nPTGlEPTB;MD60NlE{QCEQvF2= M1q2S3NCVkeHUh?=
LOXIMVI NH\l[pRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfSTWM2OD1yLkSyPFg2KM7:TR?= MYPTRW5ITVJ?
EFO-27 MlvrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWP6RpUyUUN3ME2wMlQ{ODR7IN88US=> MYnTRW5ITVJ?
A2780 MkfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVKzZ3hjUUN3ME2wMlQ{OTZ{IN88US=> NF7JZWVUSU6JRWK=
CAL-12T M4fydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nhSGlEPTB;MD60N|I5QCEQvF2= NYD4OllzW0GQR1XS
ES6 Mn7FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTBwNESxPFUh|ryP NHfMPJdUSU6JRWK=
HCE-T M3myUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPwZVFKSzVyPUCuOFQyQDdizszN NHzuUFdUSU6JRWK=
SK-MES-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjTTWM2OD1yLkS0Nlk{KM7:TR?= Mn6yV2FPT0WU
Daoy NFLCO3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWiyPVZIUUN3ME2wMlQ2OTB|IN88US=> NEixXpFUSU6JRWK=
A2058 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjoSZdKSzVyPUCuOFUyOzZizszN MV;TRW5ITVJ?
Hs-578-T MmXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\WRWlEPTB;MD60OVI4QCEQvF2= NUjjOWpYW0GQR1XS
TE-11 M1W2SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fFSWlEPTB;MD60OVI6OyEQvF2= MmnpV2FPT0WU
GI-ME-N NWLWe4FWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPCNnRKSzVyPUCuOFU1PiEQvF2= MVLTRW5ITVJ?
D-392MG NVPTU|lrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjKTWM2OD1yLkS1OlEzKM7:TR?= NYPDOJd2W0GQR1XS
MOLT-13 MkC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwNE[yPVQh|ryP NX7LRlRIW0GQR1XS
SK-NEP-1 Mni4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjhVYNKSzVyPUCuOFY1OTlizszN M1qycHNCVkeHUh?=
KS-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnDW3JPUUN3ME2wMlQ3PjB|IN88US=> MVjTRW5ITVJ?
HuP-T4 NWD6XGhuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTBwNE[4Olgh|ryP MkTqV2FPT0WU
NCI-H1563 NWXOZmJTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LBT2lEPTB;MD60O|A{PSEQvF2= NGLNdGpUSU6JRWK=
U031 M{e3OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jPSmlEPTB;MD60PFI{PCEQvF2= Mnf0V2FPT0WU
HSC-3 Ml\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLDTWM2OD1yLkS4OlI3KM7:TR?= MWDTRW5ITVJ?
HT-3 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTBwNEmwN{DPxE1? NUGxNmdEW0GQR1XS
HGC-27 MkjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwNEmxNVUh|ryP NEPrOIhUSU6JRWK=
HT-1080 Mn;4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwNEmyO{DPxE1? NEnGeIxUSU6JRWK=
RT-112 MmPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTBwNEm5NlEh|ryP MYTTRW5ITVJ?
A375 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjJTWM2OD1yLkS5PVIzKM7:TR?= MUfTRW5ITVJ?
SW626 NUn0PVh4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;DfXZFUUN3ME2wMlUxPjN|IN88US=> NGT0U4RUSU6JRWK=
SBC-5 NX65UXE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnDS3JjUUN3ME2wMlUyOzR2IN88US=> MWHTRW5ITVJ?
CAL-51 NFrpdYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkT4TWM2OD1yLkWxOFM4KM7:TR?= MUnTRW5ITVJ?
RKO NF;icHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTHb3RKSzVyPUCuOVIxOTlizszN MYnTRW5ITVJ?
HC-1 MoGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXxNnVSUUN3ME2wMlUzOTF2IN88US=> MYTTRW5ITVJ?
CHL-1 NYDz[ZNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHzTWM2OD1yLkWyOVUyKM7:TR?= M1i5V3NCVkeHUh?=
SK-N-DZ M{LqdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7GTJJKSzVyPUCuOVMxPzhizszN M1vzeHNCVkeHUh?=
SNU-423 NEK2[4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVH6[oczUUN3ME2wMlU{PDZ4IN88US=> NY\yTVR2W0GQR1XS
NB13 M4DJOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEOyenlKSzVyPUCuOVM3PTdizszN NHfjfnpUSU6JRWK=
EW-16 MmLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;je3JKSzVyPUCuOVM4PzRizszN MoDnV2FPT0WU
A704 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwNUO4PFEh|ryP MYPTRW5ITVJ?
Ca9-22 NVPvd41sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPhemVEUUN3ME2wMlU1ODd5IN88US=> M3vIW3NCVkeHUh?=
NCI-H1299 M{XrVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTaWHlJUUN3ME2wMlU2PThizszN MkTZV2FPT0WU
LB771-HNC NELlc3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIH3SFBKSzVyPUCuOVU6OzNizszN M1uwT3NCVkeHUh?=
EW-11 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnX[4tKSzVyPUCuOVY{OjZizszN NYXkU|lPW0GQR1XS
LC-2-ad NX3TSFc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPnU2h{UUN3ME2wMlU3PTJ{IN88US=> MULTRW5ITVJ?
KNS-62 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjZTWM2OD1yLkW2OVU{KM7:TR?= MWDTRW5ITVJ?
CAL-62 NIDpO45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvZTWM2OD1yLkW3NVIyKM7:TR?= NVzQNFZ3W0GQR1XS
SF126 NI\lUVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXf5RWpVUUN3ME2wMlU4PDJzIN88US=> NFy4V4dUSU6JRWK=
OMC-1 MkDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwNUe0PVch|ryP NWnrbJJOW0GQR1XS
AGS NHq4bFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTBwNUixOlIh|ryP MUfTRW5ITVJ?
EW-22 NELr[VhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoraTWM2OD1yLkW4N|c2KM7:TR?= M2[wOnNCVkeHUh?=
KP-N-YS MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jteWlEPTB;MD61PFM5OiEQvF2= NVryOYJ5W0GQR1XS
BFTC-909 M1LPUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTEVmFKSzVyPUCuOVg5OzNizszN M2TsNXNCVkeHUh?=
PSN1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPmSZhFUUN3ME2wMlU6OTF6IN88US=> MmHIV2FPT0WU
NB10 NIfv[VhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTrTWM2OD1yLkW5NVI6KM7:TR?= NYH6dZR5W0GQR1XS
COLO-800 NFfmdHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTPTWM2OD1yLk[wN|I5KM7:TR?= Mn;VV2FPT0WU
647-V MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzLNlF3UUN3ME2wMlYyPzh5IN88US=> NXTQflR7W0GQR1XS
VM-CUB-1 MkfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTBwNkG4OlEh|ryP NXm1SVVYW0GQR1XS
OS-RC-2 NULFVI1nT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVy3fYR6UUN3ME2wMlYzODFizszN NIPvNWZUSU6JRWK=
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NCI-H226 M4XhN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;aWmlEPTB;ND61OFg4OSEQvF2= MmnoV2FPT0WU
CAL-27 Mn:1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2OxbmlEPTB;ND63NVY6PCEQvF2= MnzYV2FPT0WU
G-401 NIHRelFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvWTWM2OD12LkizPFQyKM7:TR?= Mn:wV2FPT0WU
CAL-120 M{n1[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\kUpdtUUN3ME20Mlg1QTlzIN88US=> NXP5bppTW0GQR1XS
DMS-114 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTVwMUS1NFYh|ryP Mn3GV2FPT0WU
KYSE-70 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\xTWM2OD13LkG0PFU6KM7:TR?= M{HHRnNCVkeHUh?=
NCI-H1648 NG\m[2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzu[VlKSzVyPUWuNlk4QDJizszN NXvvOJFoW0GQR1XS
MDA-MB-175-VII MmPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTVwNESwNVQh|ryP NIT3TXlUSU6JRWK=
NCI-H2029 NGHKTpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTVwNEiwNlIh|ryP M3n4ZnNCVkeHUh?=
LS-1034 NH36[nFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfxTWM2OD13Lk[2PFg6KM7:TR?= NVq2[mo3W0GQR1XS
LB831-BLC MmHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PR[WlEPTB;NT63NFM1PSEQvF2= MYDTRW5ITVJ?
MDA-MB-415 M3K5U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2SwfGlEPTB;NT63NFgyKM7:TR?= MV7TRW5ITVJ?
TE-6 MnvSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTVwN{SxPFIh|ryP M37abHNCVkeHUh?=
SK-MEL-24 MlHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfzU45KSzVyPUWuO|Q2PjRizszN MnLPV2FPT0WU
HUTU-80 MkWxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXTUG9UUUN3ME21Mlc2OzN|IN88US=> MWjTRW5ITVJ?
OCUB-M M2LGPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTVwN{e1OlMh|ryP M4fUU3NCVkeHUh?=
IGR-1 NF3QXnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTlcIZFUUN3ME21Mlg3QDl2IN88US=> M1TOUnNCVkeHUh?=
NCI-H747 NUjYSotLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;WTWM2OD14LkK0OVg2KM7:TR?= NYmxUWVEW0GQR1XS
COLO-680N NXq5[FBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTZwM{m0NFMh|ryP MVTTRW5ITVJ?
NB12 NVzzNVlNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M16xUWlEPTB;Nj63PVczOiEQvF2= NULt[XVWW0GQR1XS
SNU-449 M1jsR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTZwOUm1PVEh|ryP MUDTRW5ITVJ?
D-542MG MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIj0SmtKSzVyPUeuNVIzQTlizszN NWnldnprW0GQR1XS
COLO-792 NWfOUXhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX20dnd4UUN3ME23Mlg4OTJ5IN88US=> NVXD[4pKW0GQR1XS
NCI-H810 NWfZeGNCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml:1TWM2OD16LkCyPVg1KM7:TR?= MWPTRW5ITVJ?
T47D MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfp[VhKSzVyPUiuNVYxOjRizszN M3PnUHNCVkeHUh?=
Calu-3 NV65SFJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPveIdKSzVyPUiuNlg5QDZizszN NGP2NYxUSU6JRWK=
COLO-741 MnnCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLUTWM2OD16LkmzNFk{KM7:TR?= M2nT[3NCVkeHUh?=
LU-135 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH76e3NKSzVyPUmuNFM1PTJizszN NY\aXZN7W0GQR1XS
COLO-678 NXHTNnV2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHQV4JKSzVyPUmuNlg5PDRizszN NUn3Vm85W0GQR1XS
NCI-H2126 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvLWpFKSzVyPUmuN|M6OTlizszN NXLROFNCW0GQR1XS
SK-MEL-28 Ml7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2H1VWlEPTB;OT61N|U6PiEQvF2= MlXsV2FPT0WU
MFE-280 NFrNVYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTFyLkCyNlkh|ryP MkOyV2FPT0WU
DBTRG-05MG M{nhd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjFVmJKSzVyPUGwMlQ{PjlizszN MYHTRW5ITVJ?
RCC10RGB MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX32NFc3UUN3ME2xNE42OjV3IN88US=> NYXPRY86W0GQR1XS
NTERA-S-cl-D1 M{XDWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzCRnVKSzVyPUGwMlY5PTJizszN NWqzTo5OW0GQR1XS
C3A MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Hrb2lEPTB;MUKuN|E4OiEQvF2= NVTKfFJWW0GQR1XS
MHH-ES-1 M3W4c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3K5ZmlEPTB;MUKuOVI5PCEQvF2= MY\TRW5ITVJ?
LU-139 NWr5WHpCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHKTWM2OD1zMj62N|M{KM7:TR?= NVHReYVFW0GQR1XS
KNS-42 M3\pdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTF{Lki3O|Eh|ryP NEXrRW9USU6JRWK=
MCF7 M1j1NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXufYFwUUN3ME2xN{4zOzZ3IN88US=> MoPzV2FPT0WU
SW872 M4XnRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIm4coNKSzVyPUG0MlU1PjFizszN MkXTV2FPT0WU
HT-1376 NX\WdFF[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDnXZBKSzVyPUG0Mlg2QDJizszN M3TObnNCVkeHUh?=
NCI-SNU-5 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DyTGlEPTB;MUWuNVc1OSEQvF2= MULTRW5ITVJ?
NCI-H2405 NIrIR4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTF3LkS0O|Qh|ryP MkP1V2FPT0WU
KYSE-150 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTF3Lke2NVEh|ryP MWnTRW5ITVJ?
SW837 NWHBWYsxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjtXGZ7UUN3ME2xOk4xOTJ2IN88US=> MmHwV2FPT0WU
LU-65 M1y4bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;GXXo3UUN3ME2xPU44OjZizszN MmjnV2FPT0WU
JEG-3 NWexTY1HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPTbodKSzVyPUKwMlM2PzdizszN MWTTRW5ITVJ?
SK-MEL-1 NVHHWpBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTJyLke4N|Yh|ryP M3jafXNCVkeHUh?=
SK-HEP-1 NELlTG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTJzLkW1OFEh|ryP M3TFU3NCVkeHUh?=
NCI-H1770 NIfyV5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTuTWM2OD1{Mz60NFg3KM7:TR?= Mn3NV2FPT0WU
MG-63 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DBOGlEPTB;MkOuO|Y4OyEQvF2= NF;vSlhUSU6JRWK=
ECC10 M3fkbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUOyd2s2UUN3ME2yOU42PzFizszN MWfTRW5ITVJ?
SW1463 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPTeI42UUN3ME2yO{45OzF4IN88US=> MmjNV2FPT0WU
JVM-2 MnvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zrXmlEPTB;MkiuN|I1QCEQvF2= MnPWV2FPT0WU
HCT-15 NImyfYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPnfVBKSzVyPUK4MlQyQTJizszN MnTlV2FPT0WU
CAL-72 NWXSbVd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fZZWlEPTB;M{GuOFkyOiEQvF2= NGfVVGpUSU6JRWK=
HCC2218 MnrOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknGTWM2OD1|Mj64PFU5KM7:TR?= NHjXO4pUSU6JRWK=
BT-474 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTN|LkC4OFMh|ryP NV\2fJJ3W0GQR1XS
NCI-H1092 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTN|LkWxOFUh|ryP MVXTRW5ITVJ?
Mo-T M2S3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjMTWM2OD1|Nz6zOVg2KM7:TR?= M1u3RnNCVkeHUh?=
DU-145 M2fKWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{T2S2lEPTB;NECuOlI6PSEQvF2= NHrLfZNUSU6JRWK=
NCI-H1666 M4PNUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTR{LkS0PFgh|ryP NGDTTWNUSU6JRWK=
IST-MES1 MnHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTR4LkSxPVYh|ryP M{jwcHNCVkeHUh?=
SW1116 M3;5T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4W4e2lEPTB;NEiuNVc2PiEQvF2= NWHnSGQxW0GQR1XS
MZ2-MEL NFy2VIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3[yUWlEPTB;NEiuOVAzPiEQvF2= NHy2[VZUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pChk1 / Chk1 / Wee1 / p21 / p53 ; 

PubMed: 29963769     


Immunoblotting analysis of CAL39 and SW954 cells showing increase in CHK1 phosphorylation after exposure to AZD7762 for 24 hours. β‐actin is used as loading control. Representative data from n = 3 experiments.

29963769
Growth inhibition assay
Cell viability; 

PubMed: 23715154     


Lkb1-wt (634, 855, and 857) and Lkb1-null (t2, t4, and t5) cell lines were plated into 96-well plates at 2000 cells/well in 150 ml medium containing the indicated concentrations of AZD7762 or CHIR124 for 3 days. Viable cells were then counted with Dojindo’s Cell Counting Kit-8 assay. The data represent mean ± SD for 3 repeats. GI50 was calculated with GraphPad.

23715154
In vivo AZD7762 alone at 25 mg/kg shows little antitumor activity in the H460-DNp53 xenograft mice and SW620 xenograft mice, but when administrated in combination with gemcitabine (60 mg/kg), AZD7762 shows significant antitumor efficacy in the two xenografts mice with a log cell kill of 0.9 or percent treated/control (%T/C) of 26 even at low dose of 12.5 mg. Dosing of AZD7762 in combination with gemcitabine (10 mg/kg) in the H460-DNp53 xenograft rat inhibits the tumor volume in a dose-dependent manner with the %T/C values of 48 and 32 for 10 and 20 mg/kg AZD7762, respectively. AZD7762 (25 mg/kg) in combination with irinotecan (25 or 50 mg/kg) causes complete tumor regression in the SW620 xenograft mice with the %T/C increasing significantly to -66% and -67%, respectively. [1]

Protocol

Kinase Assay:[1]
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Chk1 Kinase Assay:

Recombinant human Chk1 is expressed as a glutathione S-transferase fusion in insect cells using a baculovirus vector and purified by glutathione affinity chromatography. A synthetic peptide substrate (N-biotinylaminohexanoyl-KKVSRSGLYRSPMPENLNRPR) for Chk1 is synthesized. Final assay concentrations of peptide and ATP (cold + 40 nCi [33P]ATP) are 0.8 and 1 μM, respectively. Different concentrations of AZD7762, buffer containing peptide and chk1 kinase and ATP, are added sequentially to a 384-well assay plate. The plate is incubated for 2 hours, reaction is stopped by the addition of buffer containing EDTA and scintillation proximity assay beads, and plates are read using a TopCount reader. Data analysis is carried out to determinate a dose response (IC50).
Cell Research:[1]
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  • Cell lines: HT29, SW620 and MDA-MB-231 cells
  • Concentrations: Dissolved in DMSO, final concentration ~12.5 μM
  • Incubation Time: 20 or 48 hours
  • Method: For the checkpoint abrogation assay, HT29 cells are treated for 2 hours with camptothecin (topoisomerase I inhibitor; 0.07 μg/mL) to induce the G2 checkpoint. Cells are then treated for 20 hours with a 12-point titration of AZD7762 (12.5 μM to 6 nM) plus nocodazole. Cells are fixed with 3.7% formaldehyde for 1 hour, permeabilized with PBS containing 0.05% Triton X, and incubated with anti-phH3 antibody for 1 hour followed by Alexa Fluor 488 anti-rabbit and Hoechst stain for 1 hour. Mitotic index is determined on the ArrayScan and expressed as the percentage of cells undergoing mitosis. For the potentiation assays, SW620 or MDA-MB-231 cells are dosed for 24 hours with a 9-point titration of gemcitabine ranging from 0.01 to 100 nM with a constant dose of AZD7762 (300 nM). After 24 hours, medium is removed and AZD7762 alone is added back to the wells for an additional 24 hours. Cells are then incubated in AZD7762-free medium for an additional 72 hours. The effect on cell proliferation is determined by MTT.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Male NCr mice implanted s.c. with H460-DNp53 cells or SW620, and male rnu rats implanted s.c. with H460-DNp53 cells
  • Formulation: Formulated in 11.3% hydroxyproplyl-β-cyclodextrin
  • Dosages: ~25 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (137.96 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.42
Formula

C17H19FN4O2S

CAS No. 860352-01-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    If I want to use the inhibitor for i.p. injection, can I use DMSO to dissolve it?

  • Answer:

    Since DMSO has low toxicity with i.p. injection, hydroxyproplyl-β-cyclodextrin is consider a safer co-solvent in compound formulation. In a previous literature report, 11.3% hydroxyproplyl-β-cyclodextrin was used to formulate this compound for i.v. injection.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID