AZD7762

Catalog No.S1532

AZD7762 Chemical Structure

Molecular Weight(MW): 362.42

AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.

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Cited by 32 Publications

Purity & Quality Control

Choose Selective Chk Inhibitors

Biological Activity

Description AZD7762 is a potent and selective inhibitor of Chk1 with IC50 of 5 nM in a cell-free assay. It is equally potent against Chk2 and less potent against CAM, Yes, Fyn, Lyn, Hck and Lck. Phase 1.
Targets
Chk1 [1]
(Cell-free assay)
Chk2 [1]
(Cell-free assay)
5 nM <10 nM
In vitro

AZD7762, a more selective Chk1 inhibitor, inhibits Chk1 phosphorylation of a cdc25C peptide by reversibly binding to the ATP-binding site of Chk1, with IC50 of 5 nM and Ki of 3.6 nM. AZD7762 induces cell arrest with EC50 of 0.620 μM, and significantly abrogates the camptothecin induced G2 arrest with an EC50 of 10 nM, by blocking the chk1 dependent degradation of Cdc25A and activation of Cyclin A. AZD7762 (300 nM) enhances the antitumor efficacy of gemcitabine against SW620 and topotecan against MDA-MB-231 by reducing the GI50 values from 24.1 nM and 2.25 μM to 1.08 nM and 0.15 μM, respectively. [1] AZD7762 shows cytotoxicity against a variety of neuroblastoma cell lines bearing p53 wild type, p53 mutation, Mdm2 amplification or p14 deletion with IC50 values ranging from 82.6-505.9 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KASUMI-1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTBwMECwNlM1KM7:TR?= NG\zUoNUSU6JRWK=
NKM-1 NIP3boJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7xU|ZKSzVyPUCuNFAxPDF3IN88US=> M{DqcXNCVkeHUh?=
MV-4-11 M13aWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHBUINKSzVyPUCuNFAxPDJ3IN88US=> MV;TRW5ITVJ?
NCI-H1703 NHTzdXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnRcm1KSzVyPUCuNFA3PDNizszN M2\oenNCVkeHUh?=
A204 M13XWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIL0TIlKSzVyPUCuNFA5PTZizszN M{DyVXNCVkeHUh?=
CGTH-W-1 NYH4Uoo5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXoTWM2OD1yLkCxOFIh|ryP M3q4ZnNCVkeHUh?=
MLMA M1S3b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;GNlNKSzVyPUCuNFI5PDlizszN NWrkcYtGW0GQR1XS
GOTO NULObJkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlzETWM2OD1yLkCzNVIh|ryP NF33R|ZUSU6JRWK=
KE-37 MnnwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXXTWM2OD1yLkCzNVQzKM7:TR?= NG\xSWFUSU6JRWK=
ES4 NVXyRo5PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWP2TldLUUN3ME2wMlA{OjVzIN88US=> NV7pO3ppW0GQR1XS
ATN-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVv1eGJbUUN3ME2wMlA{PDF5IN88US=> MofaV2FPT0WU
MHH-PREB-1 NFz2[49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTBwMEO0OFIh|ryP MWrTRW5ITVJ?
SW756 NWK2TIpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTBwMEO1O|kh|ryP M{m5V3NCVkeHUh?=
CTV-1 NU[1N2o{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHGT2lKSzVyPUCuNFM5QTZizszN NIi0OndUSU6JRWK=
HCC1806 M2\Q[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfsTWM2OD1yLkC0NFIzKM7:TR?= NUPDN2FNW0GQR1XS
NEC8 M3;HVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjCTWM2OD1yLkC0NlA{KM7:TR?= M{DtW3NCVkeHUh?=
P12-ICHIKAWA M37SUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjXTWM2OD1yLkC0N|E3KM7:TR?= NEPYN4NUSU6JRWK=
DEL NIHqV5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrhUpZoUUN3ME2wMlA1PTl{IN88US=> M{fZOnNCVkeHUh?=
KM12 M2i2cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTRdmFMUUN3ME2wMlA2OTV6IN88US=> MU\TRW5ITVJ?
LB1047-RCC MkLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1j6Z2lEPTB;MD6wOlAzPSEQvF2= M3TXXnNCVkeHUh?=
LB2241-RCC NYHBZnZmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\LeohOUUN3ME2wMlA3OTB{IN88US=> NVvz[5RSW0GQR1XS
NOMO-1 M{TJVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{f4NWlEPTB;MD6wOlEyPSEQvF2= MmPwV2FPT0WU
NB14 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTBwME[xN|Yh|ryP NWTxWGR{W0GQR1XS
ONS-76 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzFZnlKSzVyPUCuNFYyQTZizszN M4j6S3NCVkeHUh?=
HD-MY-Z M3\xN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDxfmVKSzVyPUCuNFYzPTVizszN MnThV2FPT0WU
G-402 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjFV45KSzVyPUCuNFY1PiEQvF2= M3zOdHNCVkeHUh?=
ES1 NHLBblhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfhbYdKSzVyPUCuNFcyPDZizszN NIH3UHlUSU6JRWK=
L-363 Mn[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\ZTWM2OD1yLkC3N|kh|ryP NYLCdJV4W0GQR1XS
A4-Fuk NV;YeItoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3n4XWlEPTB;MD6wO|Q5PiEQvF2= MU\TRW5ITVJ?
OC-314 M2THT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLLbJp{UUN3ME2wMlA4QDB5IN88US=> Ml;uV2FPT0WU
SK-LU-1 M2jKVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfreWlKSzVyPUCuNFg1OjFizszN NX\TbW5XW0GQR1XS
MDA-MB-157 MmO2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWO0SJFuUUN3ME2wMlA5PjV7IN88US=> NFPsfWhUSU6JRWK=
HuP-T3 NVXtTG5CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4T6cmlEPTB;MD6wPFcyKM7:TR?= MWDTRW5ITVJ?
A3-KAW NEj6S2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rJSGlEPTB;MD6wPFgxPiEQvF2= MX7TRW5ITVJ?
MOLT-16 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\VW2lEPTB;MD6wPFgxPyEQvF2= MXrTRW5ITVJ?
COLO-679 MnTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3hTWM2OD1yLkC5NFkyKM7:TR?= NXLMTndiW0GQR1XS
EM-2 NV3HfHVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTBwMEm5NFMh|ryP NFHtV4lUSU6JRWK=
ES8 MnHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XROmlEPTB;MD6wPVkyQCEQvF2= NF\QWZRUSU6JRWK=
SK-MEL-2 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1L3dmlEPTB;MD6xNFA{OyEQvF2= NHLjc4VUSU6JRWK=
ES3 NIDGdI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVz0bY9HUUN3ME2wMlExOiEQvF2= NH3lSm5USU6JRWK=
HSC-4 MonxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zOV2lEPTB;MD6xNFU2PCEQvF2= Mlv6V2FPT0WU
697 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL1PXpJUUN3ME2wMlEyODZ|IN88US=> M2TpOXNCVkeHUh?=
TGBC11TKB NXrXNmJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13G[2lEPTB;MD6xNVA6OyEQvF2= M3fvS3NCVkeHUh?=
SK-PN-DW MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLqTWM2OD1yLkGxPFYh|ryP NX;DVZEzW0GQR1XS
H9 MnPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwMUKxOlUh|ryP NWjvPGNRW0GQR1XS
NCI-H2122 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnqNIE6UUN3ME2wMlEzOjVizszN MYXTRW5ITVJ?
ML-2 M2DDV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDvUYZKSzVyPUCuNVI1QDRizszN Ml;mV2FPT0WU
MC-IXC M13nd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3HdoRKSzVyPUCuNVI5PDNizszN NFnQWoVUSU6JRWK=
KYSE-140 MlnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTBwMUOwOFEh|ryP MoDPV2FPT0WU
DOHH-2 NVG5T2dCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzYcFhKUUN3ME2wMlE{ODZ2IN88US=> MXvTRW5ITVJ?
MEL-JUSO NYG0VYl4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrqWVdKSzVyPUCuNVMyOjhizszN NFzDRW1USU6JRWK=
P30-OHK NXr5ZnJWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLPbXhKSzVyPUCuNVMyPiEQvF2= M3vlVXNCVkeHUh?=
CTB-1 M2eyV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTBwMUOzNlUh|ryP MmTGV2FPT0WU
ACHN Ml;HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHTTWM2OD1yLkGzN|Y6KM7:TR?= MVPTRW5ITVJ?
ES5 NFfz[HJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LtZ2lEPTB;MD6xN|Q4PiEQvF2= NIHk[HFUSU6JRWK=
SF539 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGmwVIVKSzVyPUCuNVM3QCEQvF2= NGr5OVJUSU6JRWK=
D-283MED NXPHNGxGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LWO2lEPTB;MD6xN|Y5PyEQvF2= MXjTRW5ITVJ?
OVCAR-5 NXy1PHo5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTBwMUO4PVQh|ryP MX\TRW5ITVJ?
RPMI-8866 MlLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnrsTWM2OD1yLkGzPVI2KM7:TR?= NGfrRnBUSU6JRWK=
PANC-03-27 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4C1[mlEPTB;MD6xOFI5QCEQvF2= MkHYV2FPT0WU
M14 M3\MXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvpTJZKSzVyPUCuNVQ1PjNizszN MonaV2FPT0WU
D-566MG MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ToNGlEPTB;MD6xOFY6KM7:TR?= NVjzSGRYW0GQR1XS
Ramos-2G6-4C10 NXXhTYJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVG4U|BGUUN3ME2wMlE1QDR{IN88US=> NF3ETI1USU6JRWK=
NOS-1 NIXndnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nzWmlEPTB;MD6xOFk3PyEQvF2= MWrTRW5ITVJ?
YH-13 NHLs[lZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV25OpM6UUN3ME2wMlE2Pjd2IN88US=> NYrv[Y1{W0GQR1XS
NB17 M1LNbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;jZmlEPTB;MD6xOVc5PiEQvF2= NWq1O|hWW0GQR1XS
U-2-OS M3[2emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPCTWM2OD1yLkG1PFQ4KM7:TR?= MnrlV2FPT0WU
ES7 NFPldYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETIVVdKSzVyPUCuNVYxOTFizszN NUTQW4RvW0GQR1XS
LoVo M2m4Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTBwMU[xOlYh|ryP MkPtV2FPT0WU
QIMR-WIL NULQToN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33rZmlEPTB;MD6xOlMyOSEQvF2= NXrTfnRiW0GQR1XS
ESS-1 MoHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTxTWM2OD1yLkG2OFE{KM7:TR?= Ml;QV2FPT0WU
LAMA-84 NH\UXlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTBwMU[1NFMh|ryP M2PRPXNCVkeHUh?=
TGBC24TKB NIniUlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nJ[2lEPTB;MD6xOlU{PSEQvF2= MlvmV2FPT0WU
KU812 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTBwMU[4N{DPxE1? NGrHdoJUSU6JRWK=
KARPAS-45 MlzlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnzTWM2OD1yLkG2PFQ4KM7:TR?= NWjqSlNZW0GQR1XS
ACN MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPLWFZKSzVyPUCuNVcyPzFizszN M1qzdHNCVkeHUh?=
RPMI-8226 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mln4TWM2OD1yLkG3OFY1KM7:TR?= M4XCVXNCVkeHUh?=
GR-ST M{DhUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTBwMUe4Nlch|ryP NXm1VJlrW0GQR1XS
SK-UT-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwMUi1PVkh|ryP M3zGVXNCVkeHUh?=
SK-N-AS MkHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\5TWM2OD1yLkG4PFE2KM7:TR?= MlTvV2FPT0WU
EW-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17aNGlEPTB;MD6xPFkxPSEQvF2= MmOxV2FPT0WU
5637 NHzwXnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DYeGlEPTB;MD6xPVI4QCEQvF2= M4XyUHNCVkeHUh?=
GI-1 M4jhOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LYSGlEPTB;MD6xPVU3OSEQvF2= NHvjfFdUSU6JRWK=
NCI-H1395 NH7tT2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTBwMUm3OlEh|ryP MYDTRW5ITVJ?
CHP-212 NHfRNnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwMUm3PFIh|ryP MlzLV2FPT0WU
A427 M{jwcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofZTWM2OD1yLkG5PFMzKM7:TR?= MWDTRW5ITVJ?
BE-13 NGXLO|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DIO2lEPTB;MD6yNFEyPyEQvF2= MYHTRW5ITVJ?
RS4-11 NX73dVR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTBwMkCzNVgh|ryP NVXUcYtFW0GQR1XS
TI-73 NIe0fZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTBwMkC4O|Mh|ryP MVfTRW5ITVJ?
HAL-01 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTBwMkC5O{DPxE1? MlXIV2FPT0WU
KY821 NVLXTo1sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV74NW5UUUN3ME2wMlIyPDJ7IN88US=> MYfTRW5ITVJ?
H4 MlPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2q4PGlEPTB;MD6yNlA3OiEQvF2= MljBV2FPT0WU
EPLC-272H NVjubGpOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jvemlEPTB;MD6yNlA3QCEQvF2= MnHIV2FPT0WU
COR-L105 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPUc2pKSzVyPUCuNlIzOzVizszN MWrTRW5ITVJ?
769-P MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTBwMkKyPVgh|ryP MYnTRW5ITVJ?
HEL NFHDTodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTBwMkK0OVQh|ryP M2jJfnNCVkeHUh?=
VA-ES-BJ NX\OdIt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwMkK1OVch|ryP MnrRV2FPT0WU
IST-MEL1 NVXKS3dqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjPTIZzUUN3ME2wMlIzPTdizszN MnLxV2FPT0WU
KYSE-270 MkL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fHSGlEPTB;MD6yNlY{OyEQvF2= MVXTRW5ITVJ?
RH-1 NGfNTJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjzenpnUUN3ME2wMlIzPjZ4IN88US=> MXrTRW5ITVJ?
JVM-3 MmCyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPXTWM2OD1yLkKzNFM3KM7:TR?= MkHDV2FPT0WU
RPMI-7951 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTBwMkOwOVYh|ryP NF\3WnVUSU6JRWK=
JAR MkO2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTBwMkOyN|gh|ryP M4rGR3NCVkeHUh?=
MOLT-4 NIDUdnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTBwMkO4OUDPxE1? NHTpdINUSU6JRWK=
ALL-PO Mm\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M12wO2lEPTB;MD6yOFI1OyEQvF2= NFr4fpRUSU6JRWK=
SNU-387 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTBwMkSzN|kh|ryP M3S3PXNCVkeHUh?=
IGROV-1 MkjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlL1TWM2OD1yLkK0OFAyKM7:TR?= M{KyUXNCVkeHUh?=
MPP-89 NFrBfJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTBwMkS0OVEh|ryP NWXOS25GW0GQR1XS
T98G NWPzbpc6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDYTWM2OD1yLkK0OVY5KM7:TR?= NHXXcmFUSU6JRWK=
NCI-H510A NFeyVYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTBwMkS2NlYh|ryP MXfTRW5ITVJ?
KOSC-2 NXnyb4dyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUe3VotEUUN3ME2wMlI1PjdzIN88US=> MUPTRW5ITVJ?
NCI-H23 NFPkRW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXvTWM2OD1yLkK0O|YyKM7:TR?= M363UnNCVkeHUh?=
MC116 NYjTNHRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;uSWlEPTB;MD6yOVY2PCEQvF2= NX;0c2hHW0GQR1XS
8-MG-BA MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XDZmlEPTB;MD6yOVc3QCEQvF2= MU\TRW5ITVJ?
ABC-1 Mn7kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjaPHlKSzVyPUCuNlYxPjRizszN NYOyWW14W0GQR1XS
J-RT3-T3-5 MoLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;kNmlEPTB;MD6yOlY4QCEQvF2= MkezV2FPT0WU
NCI-SNU-1 MknFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUm2V444UUN3ME2wMlI4ODV7IN88US=> NVzsUmJzW0GQR1XS
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BB65-RCC MmPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTLSmJ7UUN3ME2wMlI4ODl5IN88US=> NY\RUI1qW0GQR1XS
NCI-H1755 NH:xNnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHwT|dmUUN3ME2wMlI4OzB2IN88US=> MUfTRW5ITVJ?
OVCAR-3 MnvpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYT0Xmo4UUN3ME2wMlI4Ozh3IN88US=> NF7BS4hUSU6JRWK=
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NCI-H209 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTBwM{KzOVMh|ryP M33NUHNCVkeHUh?=
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OVCAR-8 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3ToemlEPTB;MD6zOVU5PCEQvF2= NF7ncldUSU6JRWK=
NH-12 NH;iSWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwM{W2PVYh|ryP NIfGTmdUSU6JRWK=
COR-L88 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXH4OYlQUUN3ME2wMlM3PDh{IN88US=> NXXlTpR7W0GQR1XS
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MIA-PaCa-2 Moj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TUeWlEPTB;MD6zO|Q6PyEQvF2= NETSeYFUSU6JRWK=
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MHH-NB-11 MlLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTBwM{i5O{DPxE1? NWHZ[nVZW0GQR1XS
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KYSE-180 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLoTWM2OD1yLkSwNVUyKM7:TR?= M1nhTnNCVkeHUh?=
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PC-14 NF;UcJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDxNWJ1UUN3ME2wMlQyPjZzIN88US=> NGnoZmtUSU6JRWK=
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NCI-H661 NXPjU3RTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\WV5p4UUN3ME2wMlY{PzF{IN88US=> NYD5[5FLW0GQR1XS
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SW1710 M3z4e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTBwNkSwNlMh|ryP MnTuV2FPT0WU
GCT NY\6Rox5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW[0S3ZlUUN3ME2wMlY1QTN3IN88US=> MnPpV2FPT0WU
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LU-134-A MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwNkW2OUDPxE1? NG\Tb2tUSU6JRWK=
Becker M2LIe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXqTWM2OD1yLk[1PFA2KM7:TR?= NEjUN2RUSU6JRWK=
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SW620 NYHiXGF5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTBwNk[yNlMh|ryP MoDVV2FPT0WU
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LXF-289 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTBwNke2NlUh|ryP MmjRV2FPT0WU
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NCI-H1355 M33lSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\abHJKSzVyPUCuOlc5PTJizszN NYrJTpRSW0GQR1XS
23132-87 NU[2VVF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{j4SGlEPTB;MD62PFMxPiEQvF2= M4D6S3NCVkeHUh?=
BHY NEjY[JRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTBwNki4NFQh|ryP MW\TRW5ITVJ?
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A172 M1\HcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwNkmyNVch|ryP NGnXOmNUSU6JRWK=
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NB69 MonHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwN{C1OFkh|ryP NXvxVlF5W0GQR1XS
HCC1937 NVTH[5l{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwN{C2N|Eh|ryP M4fub3NCVkeHUh?=
HuCCT1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvTTWM2OD1yLkewO|Y2KM7:TR?= NWPsTWZ3W0GQR1XS
HH Mof5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnRdYJGUUN3ME2wMlcxPzhzIN88US=> NXX1W5N3W0GQR1XS
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CFPAC-1 MmLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjL[FJRUUN3ME2wMlczOjV5IN88US=> M1fOb3NCVkeHUh?=
HOP-62 NVvzTJRQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofPTWM2OD1yLkeyOlIh|ryP MWLTRW5ITVJ?
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SW1417 Mn:2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTBwN{O1PVMh|ryP NYHP[IVbW0GQR1XS
NCI-H1155 MlHiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDofXlKSzVyPUCuO|M5PjRizszN NIHBeVBUSU6JRWK=
CAL-54 M17CUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHiTWM2OD1yLke0NFMh|ryP MVfTRW5ITVJ?
Saos-2 NVjnUXl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTBwN{S1PFQh|ryP MkHxV2FPT0WU
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DMS-273 MkntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37xW2lEPTB;MD63OVQ2PiEQvF2= NIHwfmVUSU6JRWK=
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NB5 NHPqOXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\SU295UUN3ME2wMlc6PjVzIN88US=> MWTTRW5ITVJ?
J82 M1\TXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTLTWM2OD1yLkiwOFg4KM7:TR?= MUjTRW5ITVJ?
HDLM-2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHKzNmdKSzVyPUCuPFE{PDlizszN NF;CXlVUSU6JRWK=
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AU565 NILjUVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\0TWM2OD1yLkiyOFQh|ryP NV;yZW1kW0GQR1XS
KYSE-520 NGfNfYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrxTWM2OD1yLkiyOlM3KM7:TR?= M2jXcnNCVkeHUh?=
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KG-1 MoDLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTBwOES0PFQh|ryP NI\wWlRUSU6JRWK=
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NCI-H292 NVXxNFA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUThUI0yUUN3ME2wMlg6Pzd5IN88US=> NEfHfW5USU6JRWK=
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VMRC-RCZ M4mzT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUj1VJBvUUN3ME2wMlkxPTd6IN88US=> Mn3RV2FPT0WU
D-263MG NIe3OIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jJd2lEPTB;MD65NFk1OyEQvF2= MUHTRW5ITVJ?
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SW1573 Ml34S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTBwOUSyNlch|ryP NUfyfZNDW0GQR1XS
NCI-H1792 M2HLOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTBwOUSyN|ch|ryP NVfDSpk{W0GQR1XS
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HPAF-II MlTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwOUewNlgh|ryP MnjaV2FPT0WU
MEL-HO NHS4TmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn:yTWM2OD1yLkm3PVQ2KM7:TR?= MofhV2FPT0WU
LCLC-103H MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXxR|dKUUN3ME2wMlk5ODJ5IN88US=> MY\TRW5ITVJ?
CAL-33 MmTuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2[2UmlEPTB;MD65PFM4QCEQvF2= MVvTRW5ITVJ?
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NMC-G1 NHjw[VRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHxVGpwUUN3ME2wMlk5PTl{IN88US=> Mnz4V2FPT0WU
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COLO-320-HSR M3r3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\Jc|lKSzVyPUGuNFA1OTJizszN MXHTRW5ITVJ?
NCI-H2030 NHr1NoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jBPWlEPTB;MT6wNVgyOyEQvF2= Mnr5V2FPT0WU
SCC-4 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTFwMEG4OVkh|ryP NY\PbWN2W0GQR1XS
KYSE-410 Mki5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;Md3Q2UUN3ME2xMlA{PTJizszN NVq5eow2W0GQR1XS
NCI-H1650 NUTONHJJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nWPWlEPTB;MT6wN|k4OSEQvF2= MljpV2FPT0WU
S-117 M2jaRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrzfpRzUUN3ME2xMlA1KM7:TR?= NHf6TVVUSU6JRWK=
GAK NV31XIE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTFwMEWwO|Uh|ryP MnvCV2FPT0WU
KYSE-450 NH\2VnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLmUHJxUUN3ME2xMlA2OzN4IN88US=> MoT6V2FPT0WU
M059J MnPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYn4dZFmUUN3ME2xMlA3QDJ6IN88US=> MlzjV2FPT0WU
LAN-6 NF3XXYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1:4dGlEPTB;MT6wO|c4OyEQvF2= NVu3R3N6W0GQR1XS
NCI-H1651 MnzXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWex[INMUUN3ME2xMlA6OzV{IN88US=> MULTRW5ITVJ?
KMOE-2 NYXKeIxjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTFwMUC5O|Mh|ryP M4TkUnNCVkeHUh?=
SN12C MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTFwMUG3PFUh|ryP M3S5THNCVkeHUh?=
SW900 NVrrXpRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTFwMUKyOVEh|ryP MWLTRW5ITVJ?
TE-8 M4r3W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVqyNWh2UUN3ME2xMlEzOjlzIN88US=> NIr6cWtUSU6JRWK=
HO-1-N-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzVXHFqUUN3ME2xMlE1OzV|IN88US=> NYrxXII{W0GQR1XS
BHT-101 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1m3Z2lEPTB;MT6xOFcyPCEQvF2= NY\qSVhjW0GQR1XS
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SW780 M1zM[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\ISnA2UUN3ME2xMlE6PTF3IN88US=> M4jBZXNCVkeHUh?=
SNB75 NF7oXXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTFwMUm4NVkh|ryP M4DUVHNCVkeHUh?=
U-87-MG MkPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPwdJFKSzVyPUGuNlE{OzRizszN MlP0V2FPT0WU
TE-1 NHjHcWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7yTWM2OD1zLkKxN|Y4KM7:TR?= NIHDRlNUSU6JRWK=
CAS-1 NVnhN5RXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4SxU2lEPTB;MT6yNVQyQCEQvF2= M3jTO3NCVkeHUh?=
MKN7 NYPGbmw3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTNPWNMUUN3ME2xMlIyPDd6IN88US=> MXTTRW5ITVJ?
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NCI-H1693 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LtZWlEPTB;MT6yNlc2QCEQvF2= Moi2V2FPT0WU
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NCI-H2347 M2foZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnToTWM2OD1zLkKzOVI2KM7:TR?= MWrTRW5ITVJ?
MFM-223 M{jiRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTGbYgzUUN3ME2xMlI1OzR|IN88US=> NIjLcHNUSU6JRWK=
OCI-AML2 MlfBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2e2dWlEPTB;MT6yOlY3PiEQvF2= MWnTRW5ITVJ?
SAS MnK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTFwMk[2O|Ih|ryP NV3mcoJCW0GQR1XS
BT-549 MlvHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3znU2lEPTB;MT6yOlc5OiEQvF2= NInSOYpUSU6JRWK=
NCI-H2228 M37KbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjRTWM2OD1zLkK4OlQh|ryP Mlj4V2FPT0WU
BxPC-3 NHLlWI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTFwM{C2O|Ih|ryP MVPTRW5ITVJ?
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CAL-27 NWnzNIp{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTRwN{G2PVQh|ryP MnrxV2FPT0WU
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CAL-120 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PNU2lEPTB;ND64OFk6OSEQvF2= M{PieHNCVkeHUh?=
DMS-114 M2\5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTVwMUS1NFYh|ryP NFO0eJNUSU6JRWK=
KYSE-70 NWH1ZYVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml6wTWM2OD13LkG0PFU6KM7:TR?= NUTQe3NSW0GQR1XS
NCI-H1648 MlOyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTVwMkm3PFIh|ryP NXqzV5ltW0GQR1XS
MDA-MB-175-VII MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkG2TWM2OD13LkS0NFE1KM7:TR?= M2f0dXNCVkeHUh?=
NCI-H2029 MnnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;mTWM2OD13LkS4NFIzKM7:TR?= M4K2VXNCVkeHUh?=
LS-1034 NHvZUYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mni2TWM2OD13Lk[2PFg6KM7:TR?= M3fmbXNCVkeHUh?=
LB831-BLC MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTVwN{CzOFUh|ryP NGnZW3BUSU6JRWK=
MDA-MB-415 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2mxc2lEPTB;NT63NFgyKM7:TR?= NI\5RZFUSU6JRWK=
TE-6 NGjCOohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2raOGlEPTB;NT63OFE5OiEQvF2= NXrkNZllW0GQR1XS
SK-MEL-24 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTVwN{S1OlQh|ryP MkDBV2FPT0WU
HUTU-80 MoDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkj0TWM2OD13Lke1N|M{KM7:TR?= MkXzV2FPT0WU
OCUB-M NIHxcopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTVwN{e1OlMh|ryP Ml3UV2FPT0WU
IGR-1 M{\Uemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnwTXRRUUN3ME21Mlg3QDl2IN88US=> NW\0TXZqW0GQR1XS
NCI-H747 M1mzNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfL[5c2UUN3ME22MlI1PTh3IN88US=> MXnTRW5ITVJ?
COLO-680N M3PVXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjjTZRKSzVyPU[uN|k1ODNizszN NVv2O5JDW0GQR1XS
NB12 NEXY[4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;TZ4dKSzVyPU[uO|k4OjJizszN M325VXNCVkeHUh?=
SNU-449 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDOTWM2OD14Lkm5OVkyKM7:TR?= M1LzXnNCVkeHUh?=
D-542MG M3\hVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTdwMUKyPVkh|ryP Ml3CV2FPT0WU
COLO-792 NWXBdHFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfoZ|BKSzVyPUeuPFcyOjdizszN NF\aVldUSU6JRWK=
NCI-H810 MoPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfNWYFKSzVyPUiuNFI6QDRizszN NYft[pJzW0GQR1XS
T47D M3Ky[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRThwMU[wNlQh|ryP M1n3SXNCVkeHUh?=
Calu-3 M2S3OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjqfI5nUUN3ME24MlI5QDh4IN88US=> NG\4NXlUSU6JRWK=
COLO-741 M336[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnZc5Y1UUN3ME24Mlk{ODl|IN88US=> MX7TRW5ITVJ?
LU-135 NX3x[2g2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn30TWM2OD17LkCzOFUzKM7:TR?= MUnTRW5ITVJ?
COLO-678 M4S0T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rxTGlEPTB;OT6yPFg1PCEQvF2= MoDpV2FPT0WU
NCI-H2126 Ml7jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXKwO5k1UUN3ME25MlM{QTF7IN88US=> MYjTRW5ITVJ?
SK-MEL-28 M2fMcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37B[2lEPTB;OT61N|U6PiEQvF2= M2HKTnNCVkeHUh?=
MFE-280 MoPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfzclVKSzVyPUGwMlAzOjlizszN M3\GVHNCVkeHUh?=
DBTRG-05MG MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYn2fJJmUUN3ME2xNE41OzZ7IN88US=> M3[0WnNCVkeHUh?=
RCC10RGB NELpSHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2H6cGlEPTB;MUCuOVI2PSEQvF2= MUTTRW5ITVJ?
NTERA-S-cl-D1 MkjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTFyLk[4OVIh|ryP NH7DWYdUSU6JRWK=
C3A M{WwRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTF{LkOxO|Ih|ryP NUXhW|F4W0GQR1XS
MHH-ES-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvMTWM2OD1zMj61Nlg1KM7:TR?= NWL0VnFPW0GQR1XS
LU-139 NIDCTXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDKe2NKSzVyPUGyMlY{OzNizszN MlHtV2FPT0WU
KNS-42 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XCNWlEPTB;MUKuPFc4OSEQvF2= MlfDV2FPT0WU
MCF7 NYH6d4FwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPITWM2OD1zMz6yN|Y2KM7:TR?= MmfVV2FPT0WU
SW872 NXflO2tFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH:1cpRKSzVyPUG0MlU1PjFizszN MoHhV2FPT0WU
HT-1376 NYHGNZIxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTF2Lki1PFIh|ryP NETLT21USU6JRWK=
NCI-SNU-5 NXzXVZZJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPUTWM2OD1zNT6xO|QyKM7:TR?= MV7TRW5ITVJ?
NCI-H2405 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTF3LkS0O|Qh|ryP MYTTRW5ITVJ?
KYSE-150 MnTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;qdnRYUUN3ME2xOU44PjFzIN88US=> NELqV41USU6JRWK=
SW837 M2\Fdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLyc4FrUUN3ME2xOk4xOTJ2IN88US=> MnWyV2FPT0WU
LU-65 M17Pcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmq2TWM2OD1zOT63NlYh|ryP M4LHdHNCVkeHUh?=
JEG-3 NXX4OWZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7UTWM2OD1{MD6zOVc4KM7:TR?= NXLOdWxUW0GQR1XS
SK-MEL-1 NHvCWXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjLco9KSzVyPUKwMlc5OzZizszN NVTmcZpyW0GQR1XS
SK-HEP-1 Mni0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTvV29KSzVyPUKxMlU2PDFizszN MnThV2FPT0WU
NCI-H1770 NY\rRZZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\3TWlEPTB;MkOuOFA5PiEQvF2= MlPtV2FPT0WU
MG-63 MnrIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHGcppUUUN3ME2yN{44Pjd|IN88US=> NY\Lcm5PW0GQR1XS
ECC10 NHHhZ2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTJ3LkW3NUDPxE1? NYnRZ25RW0GQR1XS
SW1463 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTJ5LkizNVYh|ryP Mn7BV2FPT0WU
JVM-2 NVXFbWlYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjycndKSzVyPUK4MlMzPDhizszN MlfNV2FPT0WU
HCT-15 NW\IZm1mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTJ6LkSxPVIh|ryP NIPQXIdUSU6JRWK=
CAL-72 MlmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTNzLkS5NVIh|ryP NVrYWG9ZW0GQR1XS
HCC2218 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmL5TWM2OD1|Mj64PFU5KM7:TR?= NWnOcY9tW0GQR1XS
BT-474 MojHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETTWVFKSzVyPUOzMlA5PDNizszN NVL2e5d[W0GQR1XS
NCI-H1092 NHjvZ2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPOTWM2OD1|Mz61NVQ2KM7:TR?= M2jXdHNCVkeHUh?=
Mo-T M{HCdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYH0XG1YUUN3ME2zO{4{PTh3IN88US=> MnnoV2FPT0WU
DU-145 M1HR[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrBZldKSzVyPUSwMlYzQTVizszN MXzTRW5ITVJ?
NCI-H1666 NYe3XlVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYj4ZoQ5UUN3ME20Nk41PDh6IN88US=> NI\LTFBUSU6JRWK=
IST-MES1 MlzaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfjTWM2OD12Nj60NVk3KM7:TR?= M3P2VnNCVkeHUh?=
SW1116 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTR6LkG3OVYh|ryP Mk\3V2FPT0WU
MZ2-MEL NYDjSo46T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjENlM1UUN3ME20PE42ODJ4IN88US=> MoOyV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pChk1 / Chk1 / Wee1 / p21 / p53 ; 

PubMed: 29963769     


Immunoblotting analysis of CAL39 and SW954 cells showing increase in CHK1 phosphorylation after exposure to AZD7762 for 24 hours. β‐actin is used as loading control. Representative data from n = 3 experiments.

29963769
Growth inhibition assay
Cell viability; 

PubMed: 23715154     


Lkb1-wt (634, 855, and 857) and Lkb1-null (t2, t4, and t5) cell lines were plated into 96-well plates at 2000 cells/well in 150 ml medium containing the indicated concentrations of AZD7762 or CHIR124 for 3 days. Viable cells were then counted with Dojindo’s Cell Counting Kit-8 assay. The data represent mean ± SD for 3 repeats. GI50 was calculated with GraphPad.

23715154
In vivo AZD7762 alone at 25 mg/kg shows little antitumor activity in the H460-DNp53 xenograft mice and SW620 xenograft mice, but when administrated in combination with gemcitabine (60 mg/kg), AZD7762 shows significant antitumor efficacy in the two xenografts mice with a log cell kill of 0.9 or percent treated/control (%T/C) of 26 even at low dose of 12.5 mg. Dosing of AZD7762 in combination with gemcitabine (10 mg/kg) in the H460-DNp53 xenograft rat inhibits the tumor volume in a dose-dependent manner with the %T/C values of 48 and 32 for 10 and 20 mg/kg AZD7762, respectively. AZD7762 (25 mg/kg) in combination with irinotecan (25 or 50 mg/kg) causes complete tumor regression in the SW620 xenograft mice with the %T/C increasing significantly to -66% and -67%, respectively. [1]

Protocol

Kinase Assay:[1]
+ Expand

Chk1 Kinase Assay:

Recombinant human Chk1 is expressed as a glutathione S-transferase fusion in insect cells using a baculovirus vector and purified by glutathione affinity chromatography. A synthetic peptide substrate (N-biotinylaminohexanoyl-KKVSRSGLYRSPMPENLNRPR) for Chk1 is synthesized. Final assay concentrations of peptide and ATP (cold + 40 nCi [33P]ATP) are 0.8 and 1 μM, respectively. Different concentrations of AZD7762, buffer containing peptide and chk1 kinase and ATP, are added sequentially to a 384-well assay plate. The plate is incubated for 2 hours, reaction is stopped by the addition of buffer containing EDTA and scintillation proximity assay beads, and plates are read using a TopCount reader. Data analysis is carried out to determinate a dose response (IC50).
Cell Research:[1]
+ Expand
  • Cell lines: HT29, SW620 and MDA-MB-231 cells
  • Concentrations: Dissolved in DMSO, final concentration ~12.5 μM
  • Incubation Time: 20 or 48 hours
  • Method: For the checkpoint abrogation assay, HT29 cells are treated for 2 hours with camptothecin (topoisomerase I inhibitor; 0.07 μg/mL) to induce the G2 checkpoint. Cells are then treated for 20 hours with a 12-point titration of AZD7762 (12.5 μM to 6 nM) plus nocodazole. Cells are fixed with 3.7% formaldehyde for 1 hour, permeabilized with PBS containing 0.05% Triton X, and incubated with anti-phH3 antibody for 1 hour followed by Alexa Fluor 488 anti-rabbit and Hoechst stain for 1 hour. Mitotic index is determined on the ArrayScan and expressed as the percentage of cells undergoing mitosis. For the potentiation assays, SW620 or MDA-MB-231 cells are dosed for 24 hours with a 9-point titration of gemcitabine ranging from 0.01 to 100 nM with a constant dose of AZD7762 (300 nM). After 24 hours, medium is removed and AZD7762 alone is added back to the wells for an additional 24 hours. Cells are then incubated in AZD7762-free medium for an additional 72 hours. The effect on cell proliferation is determined by MTT.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Male NCr mice implanted s.c. with H460-DNp53 cells or SW620, and male rnu rats implanted s.c. with H460-DNp53 cells
  • Formulation: Formulated in 11.3% hydroxyproplyl-β-cyclodextrin
  • Dosages: ~25 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (137.96 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.42
Formula

C17H19FN4O2S

CAS No. 860352-01-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00937664 Terminated Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca July 2009 Phase 1
NCT00937664 Terminated Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca July 2009 Phase 1
NCT00473616 Terminated Advanced Solid Tumors|Cancer|Advanced Solid Malignancies AstraZeneca May 2007 Phase 1
NCT00473616 Terminated Advanced Solid Tumors|Cancer|Advanced Solid Malignancies AstraZeneca May 2007 Phase 1
NCT00413686 Completed Solid Tumors AstraZeneca December 2006 Phase 1
NCT00413686 Completed Solid Tumors AstraZeneca December 2006 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    If I want to use the inhibitor for i.p. injection, can I use DMSO to dissolve it?

  • Answer:

    Since DMSO has low toxicity with i.p. injection, hydroxyproplyl-β-cyclodextrin is consider a safer co-solvent in compound formulation. In a previous literature report, 11.3% hydroxyproplyl-β-cyclodextrin was used to formulate this compound for i.v. injection.

Chk Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID