Name: Oil Red O
Brand: Selleck Chemicals
SKU: S4498
Rating: 5 (1 reviews)

Oil red O (ORO) is a commonly used dye for the tissue staining, which recognizes nitroglycerin and cholesterol esters. Oil red O can be fluorescently lighted up upon meeting dsDNA with emission peak localized at 600 nm, although it almost does not fluoresce by itself.

Oil Red O Chemical Structure

CAS: 1320-06-5

Purity & Quality Control

Batch: S449801 DMSO] 82 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99%

99

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Biological Activity

Description	Oil red O (ORO) is a commonly used dye for the tissue staining, which recognizes nitroglycerin and cholesterol esters. Oil red O can be fluorescently lighted up upon meeting dsDNA with emission peak localized at 600 nm, although it almost does not fluoresce by itself.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05601570	Not yet recruiting	ENT Tumor	Centre Hospitalier Universitaire de Besancon	February 10 2023	--
NCT05637944	Recruiting	Trauma Chest	Azienda Usl di Bologna\|Azienda ospedaliera universitaria Careggi\|Ospedale di Circolo e Fondazione Macchi Varese\|Trauma Center Pisa\|Ospedale Santa Maria della Scaletta di Imola\|Azienda Ospedaliera di Perugia\|Policlinico San Martino Genova\|Azienda Ospedaliero-Universitaria Maggiore della Carità di Novara\|Ospedale S. Giuseppe (Empoli)\|Pronto Soccorso Ospedale Misericordia Grosseto\|Ospedale Bufalini - Cesena\|Azienda Ospedaliero-Universitaria di Padova\|ASST Papà Giovanni XXIII Bergamo\|Pronto Soccorso e Medicina d''Urgenza Latisana\|IRCCS Humanitas - Rozzano (MI)	October 1 2022	--
NCT05593718	Not yet recruiting	ENT Tumor	Centre Hospitalier Universitaire de Besancon	October 20 2022	--

References

[1] Yang Liu, Zhijun Chen. Talanta. 2019 Nov 1;204:337-343.

Chemical Information & Solubility

Molecular Weight	408.49	Formula	C₂₆H₂₄N₄O
CAS No.	1320-06-5	SDF	--
Smiles	CC1=CC(=C(C)C=C1)N=NC2=C(C)C=C(N=NC3=C(O)C=CC4=CC=CC=C34)C(=C2)C
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 82 mg/mL ( (200.73 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

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