ODQ

Catalog No.S9796 Batch:S979601

Technical Data

Formula	C₉H₅N₃O₂
Molecular Weight	187.15	CAS No.	41443-28-1
Solubility (25°C)*	In vitro	DMSO	37 mg/mL (197.7 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

ODQ (1H-ODQ) is a potent and selective inhibitor of soluble guanylyl cyclase (sGC, nitric oxide-activated enzyme) that enhances the pro-apoptotic effects of cisplatin in human mesothelioma cells.

Targets

sGC ^[1]

In vitro

In vitro, administration of H2O2 (for 4 hrs) to rat cardiomyoblasts decreases mitochondrial respiration attributable to generation of hydroxyl radicals. Pretreatment of cells with ODQ does not abolish this cell injury. In addition, ODQ does not scavenge superoxide anions.^[1]

In vivo

Pretreatment of rats with ODQ attenuates the renal dysfunction, lung injury, and hepatocellular injury caused by lipoteichoic acid/peptidoglycan or lipopolysaccharide.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines H9c2 cells Concentrations 0.1 μM to 1 mM Incubation Time 2 hrs Method H2O2 is a useful tool for studying the effects of reactive oxygen species on different organs. It can readily diffuse across cell membranes and can be converted into the toxic hydroxyl radical at intracellular sites via the Fenton reaction in the presence of transition metals. Cells were preincubated (2 hrs, 37°C) with ODQ (0.1 mM to 1 mM), saline, or DMSO (media containing 10% DMSO). The cells then are exposed to media or H2O2 (1 mM for 4 hrs at 37°C) after which time cell injury is assessed. All experiments are carried out in duplicate of n = 4 observations.
Animal Study:^{^[1]}	Animal Models male Wistar rats Dosages 2 mg/kg Administration IP

Cell Assay:^{^[1]}

Cell lines
H9c2 cells
Concentrations
0.1 μM to 1 mM
Incubation Time
2 hrs
Method
H2O2 is a useful tool for studying the effects of reactive oxygen species on different organs. It can readily diffuse across cell membranes and can be converted into the toxic hydroxyl radical at intracellular sites via the Fenton reaction in the presence of transition metals. Cells were preincubated (2 hrs, 37°C) with ODQ (0.1 mM to 1 mM), saline, or DMSO (media containing 10% DMSO). The cells then are exposed to media or H2O2 (1 mM for 4 hrs at 37°C) after which time cell injury is assessed. All experiments are carried out in duplicate of n = 4 observations.

Animal Study:^{^[1]}

Animal Models
male Wistar rats
Dosages
2 mg/kg
Administration
IP

References

[1] K Zacharowski, et al. Crit Care Med. 2001 Aug;29(8):1599-608.

Selleck's ODQ has been cited by 1 publication

Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC -soluble guanylate cyclase - cGMP -cyclic guanosine monophosphate - PKG -protein kinase G pathway [ J Ethnopharmacol, 2023, 300:115705]	PubMed: 36099983

Zhenwu decoction ameliorates cardiac hypertrophy through activating sGC -soluble guanylate cyclase - cGMP -cyclic guanosine monophosphate - PKG -protein kinase G pathway [ J Ethnopharmacol, 2023, 300:115705]

PubMed: 36099983

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.