research use only

Nor-NOHA acetate Arginase inhibitor

Name: Nor-NOHA acetate
Brand: Selleck Chemicals
SKU: S9737
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S9737

Nor-NOHA acetate (Nω-Hydroxy-nor-L-Arginine acetate, N-OMega-hydroxy-L-norarginine acetate, N-Hydroxy-nor-L-arginine acetate) is a reversible, competitive inhibitor of arginase. Nor-NOHA inhibits arginase activity in aorta with an IC50 of less than 1 μM, that is, similar to its effects on liver arginase (IC50 = 0.5 μM).

Chemical Structure

Molecular Weight: 296.28

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: >97%

Related Targets	Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor TNF-alpha ROS Parasite PD-1/PD-L1 TLR Prostaglandin Receptor IFN CXCR Nrf2 NOS CCR NLRP3 eIF STING AhR IL Receptor NADPH-oxidase Complement System IRAK β-lactamase IDO/TDO PGES FKBP
Related Products	BEC HCl DL-Norvaline Arginase inhibitor 1 Arginase-1 Antibody [N1K20] Arginase-2 Antibody [L5B23] Arginase 1 Antibody [B11F1]

Chemical Information, Storage & Stability

Molecular Weight	296.28	Formula	C₉H₂₀N₄O₇	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1140844-63-8	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Nω-Hydroxy-nor-L-Arginine acetate salt, N-OMega-hydroxy-L-norarginine acetate, N-Hydroxy-nor-L-arginine acetate			Smiles	CC(O)=O.CC(O)=O.NC(CCN/C(N)=N/O)C(O)=O

Solubility

In vitro
Batch:

Water : 14 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.830mg/ml (2.80mM)	Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.830mg/ml (2.80mM)	Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	liver arginase ^[1] (Cell-free assay) 0.5 μM
In vitro	Nor-NOHA is a very potent competitive inhibitor of rat liver arginase in vitro with the Ki of 0.5 μM.^[2]
In vivo	Nor-NOHA significantly prevents the increase in IL-6 and VEGF levels and improves vascular function in Adjuvant-induced arthritis (AIA) rats.^[1]
References	[1] https://pubmed.ncbi.nlm.nih.gov/22647483/ [2] https://pubs.acs.org/doi/10.1021/ja970285o

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):