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Nitrotetrazolium Blue chloride Dyes chemical

Name: Nitrotetrazolium Blue chloride
Brand: Selleck Chemicals
SKU: E4025
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.E4025

Nitrotetrazolium Blue chloride (Nitro blue tetrazolium chloride, NBT) is a chromogenic substrate used for detecting oxygen metabolites in cells, staining histological tissue sections, and labeling probe-target interactions. Nitrotetrazolium blue chloride is paired with 5-bromo-4-chloro-3-inolyl phosphate (PCIB) to detect alkaline phosphatase activity.

Chemical Structure

Molecular Weight: 817.64

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: E402501 Ethanol]5 mg/mL]false]DMSO]4 mg/mL]false]Water]Insoluble]false Purity: 99.11%

99.11

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Chemical Information, Storage & Stability

Molecular Weight	817.64	Formula	C₄₀H₃₀N₁₀O₆.2Cl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	298-83-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

Ethanol : 5 mg/mL

DMSO : 4 mg/mL (4.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.200mg/ml (0.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.200mg/ml (0.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

References	[1] https://pubmed.ncbi.nlm.nih.gov/17612300/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05549453	Enrolling by invitation	Usability of the Graphical User Interface of the Device for Its Intended Use	Nexstim Ltd	September 26 2022	Not Applicable
NCT04459962	Completed	Covid-19 Infection	Ancon Technologies Ltd	June 29 2020	Not Applicable
NCT04824040	Enrolling by invitation	Dysferlinopathy\|Miyoshi Myopathy\|LGMDR2\|DMAT	Human Stem Cell Institute Russia\|Genotarget	January 15 2020	--
NCT03113747	Unknown status	Second- or Third-degree Burns	A.A. Partners LLC	March 25 2015	Phase 1\|Phase 2
NCT02211560	Terminated	Mild Cognitive Impairment	Enzymotec	September 2014	Not Applicable

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