Name: Nile Red
Brand: Selleck Chemicals
SKU: S6818
Rating: 5 (2 reviews)

Nile Red (Nile Blue A oxazone, Phenoxazone 9) is an excellent vital fluorescent stain for the detection of intracellular lipid droplets in the presence of a hydrophobic environment. Nile Red is applied for staining intracellular lipids, hydrophobic domains of proteins and lysosomal phospholipid inclusions.

Nile Red Chemical Structure

CAS: 7385-67-3

Selleck's Nile Red has been cited by 2 publications

Purity & Quality Control

Batch: S681801 DMSO] 40 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.01%

99.01

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Biological Activity

Description

Nile Red (Nile Blue A oxazone, Phenoxazone 9) is an excellent vital fluorescent stain for the detection of intracellular lipid droplets in the presence of a hydrophobic environment. Nile Red is applied for staining intracellular lipids, hydrophobic domains of proteins and lysosomal phospholipid inclusions.

Targets

lipid droplet ^[1]

In vitro
In vitro	1. Preparation of Phalloidin-TRITC working solution 1.1Preparation of the stock solution Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Phalloidin-TRITC working solution Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution. Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes. c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d.Wash twice with PBS, 5 minutes each time. e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a.Culture adherent cells on sterile coverslips. b.Remove the coverslip from the medium and aspirate excess medium. c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes. d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT01420328	Unknown status	Inflammation	Kaleida Health	May 2011	Phase 3

References

[1] Greenspan P, et al. J Cell Biol. 1985 Mar;100(3):965-73.

Chemical Information & Solubility

Molecular Weight	318.37	Formula	C₂₀H₁₈N₂O₂
CAS No.	7385-67-3	SDF	--
Smiles	CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2
Storage (From the date of receipt)	3 years -20°C(in the dark) powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C(in the dark) powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 40 mg/mL ( (125.63 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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