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MYLS22

MYLS22 is a first-in-class and selective inhibitor of optic atrophy 1 (OPA1) with anti-angiogenesis and anti-cancer activity.

MYLS22 Chemical Structure

MYLS22 Chemical Structure

CAS: 306959-01-3

Selleck's MYLS22 has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.91%
99.91

MYLS22 Related Products

Biological Activity

Description MYLS22 is a first-in-class and selective inhibitor of optic atrophy 1 (OPA1) with anti-angiogenesis and anti-cancer activity.
Targets
OPA1 [1]
In vitro
In vitro

Opa1 inhibitor MYLS22 can result in higher maximal and residual cytoplasmic calcium levels, and alter T cell receptor (TCR) signaling in thymocytes with higher susceptibility to apoptosis.<sup><a class="sref" href="#s_ref">[2]</a></sup>
Cell Research Cell lines Jurkat cells
Concentrations 50 μM
Incubation Time 2 h
Method

Jurkat cells are cultured in 1640 media supplemented with 10% FCS, 4 mM L-glutamine, 1 penicillin/streptomycin, and 55 μM β-mercaptoethanol under 5% CO2, atmospheric oxygen, at 37 °C in a humidified incubator. For MYLS22 treatment, cells are ished and resuspended in RPMI supplemented with 2% FCS and 50 μM MYLS22 for 2 h before the experiment is started.The treated cells are used for Calcium measurements, Flow cytometry, Electron microscopy, and Western blotting.
In Vivo
In vivo

MYLS22, a small molecule first-in-kind Opa1-specific inhibitor, can affect angiogenesis, tumor growth and metastasis by impinging on NF-κB activity and on angiogenic gene expression.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Animal Research Animal Models C57BL6/J mice(Opa1+/ΔEC, Opa1iΔEC, Opa1+/iΔEC, Opa1+/ΔEC, Mfn1iΔEC)
Dosages 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 443.52 Formula

C24H21N5O2S
CAS No. 306959-01-3 SDF --
Smiles CN1N(C(=O)C(=C1C)NC(=O)C2=CC3=C(S2)[N](N=C3C)C4=CC=CC=C4)C5=CC=CC=C5
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 89 mg/mL ( (200.66 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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