ML-7 HCl

For research use only.

Catalog No.S8388

10 publications

ML-7 HCl Chemical Structure

CAS No. 110448-33-4

ML-7 is an inhibitor of smooth muscle myosin light chain kinase (MLCK) with a Ki value of 0.3 µM and displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. ML-7 also inhibits PKA and PKC with Ki of 21 μM and 42 μM, respectively.

Selleck's ML-7 HCl has been cited by 10 publications

3 Customer Reviews

  • Platelets (3 × 108/mL) were treated with ML-7 (5 µM) for 20 minutes, followed by stimulation with oxLDL (50 µg/mL) for 15 seconds. Samples were then lysed, separated by SDS-PAGE, and immunoblotted for phospho-MLCSer19 followed by reprobing for β-tubulin. (Ai) Representative blots. (Aii) Densitometric analysis of 5 independent experiments. *P < .05.

    Blood, 2013, 122(4):580-9. ML-7 HCl purchased from Selleck.

    Immunofluorescent microscopy of apical F-actin (green) and ZO-1 (red) in control and KRIT1 knockdown monolayers. Scale bar, 10 mm. b-gal, b-galactosidase.

    FASEB J, 2018, doi:10.1096/fj.201800343R. ML-7 HCl purchased from Selleck.

  • Representative Hoechst 33342 (blue) and phosphorylated MLC (red) stained images of SK-LMS-1 cells. Scale bar, 200 μm.

    Biomed Pharmacother, 2017, 92:810-818. ML-7 HCl purchased from Selleck.

Purity & Quality Control

Choose Selective MLCK Inhibitors

Biological Activity

Description ML-7 is an inhibitor of smooth muscle myosin light chain kinase (MLCK) with a Ki value of 0.3 µM and displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. ML-7 also inhibits PKA and PKC with Ki of 21 μM and 42 μM, respectively.
Targets
MLCK [3]
(Cell-free assay)
PKA [4]
(in Ehrlich cells)
PKC [4]
(in Ehrlich cells)
0.3 μM(Ki) 21 μM 42 μM
In vitro

Inhibition of MLCK by ML-7 induces activation of caspase-3 in adherent MCF-10A and MCF-10A Ras-transformed cells[2]. Its treatment results in a dose-dependent decrease in MLC20 phosphorylation and a corresponding increase in cell death of SMC(smooth muscle cells). The inhibitory effect of ML-7 on MLCK is highly selective. The Ki of ML-7 for MLCK is 0.3 μM, while its Ki for protein kinase A is 21 μM and for protein kinase C is 42 μM. Overexpressing Bcl-2 can protect cells against apoptosis induced by ML-7[4].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Vero cells NXW1Wlh2SW62aY\pdoFtKGG|c3H5 M{TjV|Mh\GG7cx?= MXrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDE[Y5ofWVidnnyeZMhcW6oZXP0[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bi\G2rbnnzeIVz\WRiYX\0[ZIhfmm{YXygZ4hidGynbnflJIFnfGW{IEOg[IF6eyCkeTD2bZJidCCybHHxeYUh[XO|YYm= M1z1VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5M{[wOlc3Lz5zN{O2NFY4PjxxYU6=
neural precursor cells M1LvNWZ2dmO2aX;uJIF{e2G7 MmfNTY5pcWKrdHnvckBw\iCwZYXyc5NxcGW{ZTDwdo9tcW[ncnH0bY9vKG:oIH3veZNmKG6ndYLhcEBxemWldYLzc5Ih[2WubIOgZpkhVVSWIHHzd4F6 M2rrOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG3OlMyLz5zN{SxO|Y{OTxxYU6=

... Click to View More Cell Line Experimental Data

In vivo ML7 is able to improve Vascular endothelial dysfunction(VED) and atherosclerosis(AS) by regulating the expression of the tight junction (TJ) proteins zona occludens (ZO)-1 and occludin via mechanisms involving MLCK and MLC phosphorylation in high-fat diet-fed rabbits. ML7 decreases the expression of MLCK and MLC phosphorylation in the arterial wall of rabbits fed a high-fat diet and reduces lipid deposition lesions in AS rabbits[1].

Protocol

Cell Research:[2]
- Collapse
  • Cell lines: MCF-10A cells, MDCK cells
  • Concentrations: 40 μM
  • Incubation Time: 16 h
  • Method: Cells are treated with vehicle alone, Blebbistatin, ML-7, ML-9, latrunculin A or Y-27632 in media without serum at varying concentrations and time points before being fixed or prepared for FACS analysis. Untreated cells are also serum starved for the duration of the inhibitor treatment. Stock solutions of each agent are made in DMSO (50 μM Blebbistatin, 5 mM LA), 50% ethanol (10 mM ML-7), 70% ethanol (20 mM ML-9) or water (20 mM Y-27632) and are maintained at -20°C.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 90 mg/mL (198.78 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 452.74
Formula

C15H17IN2O2S.HCl

CAS No. 110448-33-4
Storage powder
in solvent
Synonyms N/A
Smiles C1CNCCN(C1)S(=O)(=O)C2=CC=CC3=C2C=CC=C3I.Cl

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00190242 Completed Drug: group1|Drug: group2 HIV Infection Assistance Publique - Hôpitaux de Paris|Ensemble contre le SIDA|GlaxoSmithKline June 2003 Phase 3

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID