Home Cytoskeletal Signaling MLCK inhibitor 2,3-Butanedione-2-monoxime

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2,3-Butanedione-2-monoxime MLCK inhibitor

Cat.No.S3699

2,3-Butanedione monoxime (BDM, Diacetyl monoxime, Diacetylmonoxime) is the well-characterized, low-affinity, non-competitive inhibitor of skeletal muscle myosin-II and inhibits skeletal and cardiac muscle contraction.
2,3-Butanedione-2-monoxime MLCK inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 101.10

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Quality Control

Batch: S369901 DMSO]20 mg/mL]false]Water]20 mg/mL]false]Ethanol]20 mg/mL]false Purity: 99.16%
99.16

Solubility

In vitro
Batch:

DMSO : 20 mg/mL (197.82 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 20 mg/mL

Ethanol : 20 mg/mL

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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 101.10 Formula

C4H7NO2

 Storage (From the date of receipt)
CAS No. 57-71-6 Download SDF Storage of Stock Solutions

Synonyms Diacetyl monoxime, Diacetylmonoxime Smiles CC(=NO)C(=O)C

Mechanism of Action

Targets/IC50/Ki
skeletal muscle myosin-II
In vitro
BDM inhibits skeletal muscle myosin-II, but it does not inhibit the ATPase activity of the other myosins. BDM has a broad effect on many non-myosin proteins (many uncharacterized), and thus should not be used in whole-cell experiments as a myosin inhibitor. BDM inhibits the L-type Ca2+-channel and reduces the opening probability, thereby increasing the closed time of this particular channel. BDM drives nonspecific protein dephosphorylation of many cellular proteins, including ion channels.
In vivo
BDM significant lowers the blood pressure in normo- and hypertensive rats. BDM has been shown to improve right atrial function in a porcine model of allogenic heart transplantation.
References

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05391776 Unknown status
Osteoporosis
HJB (Hangzhou) Co. Ltd.
 April 28 2022 Phase 1
NCT01721187 Completed
Fasting|Fed
Duke-NUS Graduate Medical School|Singapore Institute for Clinical Sciences
 October 2012 --

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