2,3-Butanedione-2-monoxime

Catalog No.S3699 Synonyms: Diacetyl monoxime, Diacetylmonoxime

For research use only.

2,3-Butanedione monoxime (BDM, Diacetyl monoxime, Diacetylmonoxime) is the well-characterized, low-affinity, non-competitive inhibitor of skeletal muscle myosin-II and inhibits skeletal and cardiac muscle contraction.

2,3-Butanedione-2-monoxime Chemical Structure

CAS No. 57-71-6

Purity & Quality Control

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Biological Activity

Description 2,3-Butanedione monoxime (BDM, Diacetyl monoxime, Diacetylmonoxime) is the well-characterized, low-affinity, non-competitive inhibitor of skeletal muscle myosin-II and inhibits skeletal and cardiac muscle contraction.
Targets
skeletal muscle myosin-II [1]
()
In vitro

BDM inhibits skeletal muscle myosin-II, but it does not inhibit the ATPase activity of the other myosins. BDM has a broad effect on many non-myosin proteins (many uncharacterized), and thus should not be used in whole-cell experiments as a myosin inhibitor[1]. BDM inhibits the L-type Ca2+-channel and reduces the opening probability, thereby increasing the closed time of this particular channel. BDM drives nonspecific protein dephosphorylation of many cellular proteins, including ion channels[2].

In vivo BDM significant lowers the blood pressure in normo- and hypertensive rats. BDM has been shown to improve right atrial function in a porcine model of allogenic heart transplantation[2].

Protocol (from reference)

Animal Research:[2]
  • Animal Models: Male SD rats
  • Dosages: 250 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 20 mg/mL
(197.82 mM)
Water 20 mg/mL
(197.82 mM)
Ethanol 20 mg/mL
(197.82 mM)

Chemical Information

Molecular Weight 101.10
Formula

C4H7NO2

CAS No. 57-71-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=NO)C(=O)C

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01721187 Completed -- Fasting|Fed Duke-NUS Graduate Medical School|Singapore Institute for Clinical Sciences October 2012 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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