JC-1

For research use only.

Catalog No.S9784 Synonyms: CBIC2, NK 1420

JC-1 Chemical Structure

CAS No. 3520-43-2

JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.

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Biological Activity

Description JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.
In vitro

JC-1, the mitochondrial potential (ΔΨm) marker, and high-resolution imaging are used to analyze mitochondria in cultured rat hippocampal astrocytes. Ratiometric detection of JC-1 fluorescence identifies mitochondria with high and low ΔΨm. Mitochondrial density is highest in the perinuclear region, whereas ΔΨm tends to be higher in peripheral mitochondria.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: hippocampal neurons and glial cells
  • Concentrations: 1 μg/ml
  • Incubation Time: 15 min
  • Method:

    JC-1 is dissolved in dimethylsulfoxide (DMSO) as a 2 mg/ml stock. JC-1 is excited at 490 nm and an optical image splitter device is attached to the microscope to separate spectrally the green and red components of JC-1 fluorescence.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 33 mg/mL (58.84 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 560.77
Formula

C25H27Cl5N4

CAS No. 3520-43-2
Storage powder
in solvent
Synonyms CBIC2, NK 1420

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03161561 Completed Other: capacity of primary phagocytes COPD Sheffield Teaching Hospitals NHS Foundation Trust November 16 2011 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID