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JC-1 Dyes chemical

Name: JC-1
Brand: Selleck Chemicals
SKU: S9784
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S9784

JC-1 (CBIC2, NK 1420), a fluorescent lipophilic carbocyanine dye, is a mitochondrial potential (ΔΨ(m)) marker. This compound's fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers.

Chemical Structure

Molecular Weight: 560.77

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S978401 DMSO]33 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.34%

99.34

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Chemical Information, Storage & Stability

Molecular Weight	560.77	Formula	C₂₅H₂₇Cl₅N₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	3520-43-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

DMSO : 33 mg/mL (58.84 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro

1. Take the 6-well plate as an example for cell planking, and the density is 5×10^5/mL. Incubate overnight in 5% CO2 incubator at 37℃.
Note: it is suggested that the cell density during apoptosis induction should not exceed 1×10^6/ml, which can also be cultured to the appropriate density according to your own cell type.
2. Take 0.5 mL suspension into sterile centrifuge tube; 400 g centrifugation for 3-5 min; Discard the supernatant.
3. The cells were resuspended with 1ml of this compound working solution and incubated in 5% CO2 incubator at 37℃for 15-30 min.
4. Centrifugation at room temperature for 5 min at 400 g; Suck of the supernatant.
5. The cells were resuspended with 2 mL cell culture medium or buffer, and then centrifuged at room temperature for 5 min at 400 g; Discard the supernatant and repeat twice.
6. Resuspend the cells with 1mL of fresh culture medium or buffer, and immediately conduct subsequent flow cytometry or fluorescence microscope observation.
7. Data analysis (flow cytometry) : mitochondria of healthy cells containing red aggregates of this compound were detected by FL2 channel; Apoptotic or unhealthy cells containing green monomer of this compound were detected by FL1 (FITC) channel.
8. If used for enzyme labeling instrument, use 300 μL buffer resuspended cells; Then 100 per hole μ Transfer the stained cells to a light tight 96 well plate with the amount of L, and then conduct fluorescent enzyme label plate analysis.

References

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03161561	Completed	COPD	Sheffield Teaching Hospitals NHS Foundation Trust	November 16 2011	Not Applicable

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